Transcriptome Profiles Reveal Key Regulatory Networks during Single and Multifactorial Stresses Coupled with Melatonin Treatment in Pitaya (Selenicereus undatus L.) DOI Open Access

Aamir Ali Khokhar,

Hui Liu,

Darya Khan

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8901 - 8901

Published: Aug. 15, 2024

In response to evolving climatic conditions, plants frequently confront multiple abiotic stresses, necessitating robust adaptive mechanisms. This study focuses on the responses of Selenicereus undatus L. both individual stresses (cadmium; Cd, salt; S, and drought; D) their combined applications, with an emphasis evaluating mitigating effects (M) melatonin. Through transcriptome analysis, this identifies significant gene expression changes regulatory network activations. The results show that stress decreases pitaya growth rates by 30%, reduces stem cladode development 40%, increases Cd uptake under single 50% 70%, respectively. Under enhanced activities H2O2, POD, CAT, APX, SOD elevated proline content indicate strong antioxidant defenses. We identified 141 common DEGs related tolerance, most which were AtCBP, ALA, CBP pathways. Interestingly, production genes signal transduction hormones, including abscisic acid auxin, was also significantly induced. Several calcium-dependent protein kinase regulated during M treatments. Functional enrichment analysis showed enriched metabolism, MAPK signaling, photosynthesis. addition, weighted co-expression (WGCNA) critical transcription factors (WRKYs, MYBs, bZIPs, bHLHs, NACs) associated activities, particularly within salmon module. provides morpho-physiological insights into pitaya’s suggests molecular breeding techniques enhance plant resistance.

Language: Английский

Nutritional compounds and risk assessment of mycotoxins in ecological and conventional nuts DOI Creative Commons
Bożena Łozowicka, Piotr Kaczyński, Piotr Iwaniuk

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 458, P. 140222 - 140222

Published: June 25, 2024

This comprehensive study aimed to determine the level of nutritional compounds (20 amino acids, 11 phenolic and 8 vitamins) hazard (14 mycotoxins) in ten types conventional ecological nuts from 25 countries. Moreover, chronic acute toxicological risk assessment mycotoxins was performed. Examined constituents were determined using LC-MS/MS. Ecological pine showed highest acids (233.87 g kg

Language: Английский

Citations

15

Melatonin-Mediated Molecular Responses in Plants: Enhancing Stress Tolerance and Mitigating Environmental Challenges in Cereal Crop Production DOI Open Access
Ihsan Muhammad, Shakeel Ahmad, Weijun Shen

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(8), P. 4551 - 4551

Published: April 21, 2024

Cereal crops are crucial for global food security; however, they susceptible to various environmental stresses that significantly hamper their productivity. In response, melatonin has emerged as a promising regulator, offering potential benefits stress tolerance and crop growth. This review explores the effects of on maize, sorghum, millet, rice, barley, wheat, aiming enhance resilience stress. The application shown outcomes, improving water use efficiency reducing transpiration rates in millet under drought conditions. Furthermore, it enhances salinity heavy metal by regulating activity stress-responsive genes. Similarly, sorghum its resistance high temperatures, low humidity, nutrient deficiency, potentially involving modulation antioxidant defense aspects related photosynthetic Melatonin also exerts protective against drought, salinity, metal, extreme waterlogging barley decreasing reactive oxygen species (ROS) production through system. molecular reactions upregulated photosynthesis, mechanisms, metabolic pathway, genes downregulated susceptibility conclusion, serves versatile tool cereal crops, bolstering promoting sustainable development. Further investigations warranted elucidate underlying mechanisms refine techniques fully harness role systems.

Language: Английский

Citations

11

Physiological and Biochemical Mechanisms of Wood Vinegar-Induced Stress Response against Tomato Fusarium Wilt Disease DOI Creative Commons

Hongyin Zhou,

Kejian Fu,

Yan Shen

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(2), P. 157 - 157

Published: Jan. 6, 2024

Wood vinegar, a by-product of charcoal biomass pyrolysis, has been used as biofungicide in plant disease management because its antimicrobial properties. However, the physiological and biochemical mechanisms through which wood vinegar alleviates biotic stress are poorly understood. In this study, pot experiments were conducted to investigate resistance regulation mechanism prepared from different raw materials (ZM) single material (SM) controlling tomato (

Language: Английский

Citations

8

Pesticide residues in common and herbal teas combined with risk assessment and transfer to the infusion DOI
Piotr Kaczyński, Piotr Iwaniuk, Magdalena Jankowska

et al.

Chemosphere, Journal Year: 2024, Volume and Issue: unknown, P. 143550 - 143550

Published: Oct. 1, 2024

Language: Английский

Citations

7

Metabolomics and Physiological Methods Revealed the Effects of Drought Stress on the Quality of Broomcorn Millet during the Flowering Stage DOI Creative Commons

Jiangling Ren,

Yuhan Liu,

Jiao Mao

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(2), P. 236 - 236

Published: Jan. 23, 2024

The flowering stage is a critical period for water sensitivity and quality formation of broomcorn millets. However, the effects mechanisms drought stress on millets are not clear. We used drought-resistant variety Hequ red millet (H) drought-sensitive Yanshu No. 10 (Y) were as materials treatment during stage, metabolomics physiological methods to study differences in protein, starch, amino acids, medium medium-long chain fatty their response characteristics millet. results showed that different genotypes exhibited face stress. In millet, significantly increased contents amylopectin (2.57%), pyridoxine (31.89%), anthocyanin, decreased water-soluble protein (5.82%), glutelin (10.07%), thiamine (14.95%) nicotinamide (23.01%). 10, amylose by 6.05%, riboflavin 21.11% 32.59%. Correlation analysis total starch highly positively correlated with methyl palmitate; negatively amylopectin, vitamins, proteins, free acids; was riboflavin, pyridoxine. Water-soluble most nicotinamide. Thiamine significant positive correlation negative Riboflavin nicotinamide, pyridoxine, protein. transforms into consuming glutelin, improves resistance accumulating changes its physicochemical properties decreasing content elevating amylopectin. resisted catabolizing lipids produce acids conversion other metabolites. present helps understand nutritional at provides theoretical basis selection breeding superior varieties research.

Language: Английский

Citations

5

The effect of the multi-stage process of wheat beer brewing on the behavior of pesticides according to their physicochemical properties DOI
Piotr Kaczyński, Piotr Iwaniuk, Izabela Hrynko

et al.

Food Control, Journal Year: 2024, Volume and Issue: 160, P. 110356 - 110356

Published: Feb. 2, 2024

Language: Английский

Citations

5

Effect of Plant Growth-Promoting Bacteria on Antioxidant Status, Acetolactate Synthase Activity, and Growth of Common Wheat and Canola Exposed to Metsulfuron-Methyl DOI Creative Commons
М. Д. Бакаева, С. П. Четвериков,

Sergey N. Starikov

et al.

Journal of Xenobiotics, Journal Year: 2024, Volume and Issue: 14(1), P. 79 - 95

Published: Jan. 2, 2024

Metsulfuron-methyl, a widely used herbicide, could cause damage to the sensitive plants in crop-rotation systems at extremely low levels soil. The potential of plant growth-promoting bacteria (PGPB) for enhancing resistance against herbicide stress has been discovered recently. Therefore, it is poorly understood how physiological processes occur plants, while PGPB reduce phytotoxicity herbicides agricultural crops. In greenhouse studies, effect strains Pseudomonas protegens DA1.2 and chlororaphis 4CH on oxidative damage, acetolactate synthase (ALS), enzymatic non-enzymatic antioxidants canola (Brassica napus L.), wheat (Triticum aestivum L.) were investigated under two (0.05 0.25 mg∙kg-1) metsulfuron-methyl using spectrophotometric assays. inoculation herbicide-exposed with significantly increased shoots fresh weight (24-28%), amount glutathione GSH (60-73%), flavonoids (5-14%), as well activity ascorbate peroxidase (129-140%), superoxide dismutase SOD (35-49%), ALS (50-57%). Bacterial treatment stimulated (37-94%), (65-73%), reductase (19-20%), accumulation (61-261%), (17-22%), (27-33%) canola. Simultaneous prevented lipid peroxidation induced by plants. Based findings, possible that protective role bacterial metsulfuron-metil linked antioxidant system activation.

Language: Английский

Citations

4

Mitigating secondary salinization in grapes: long-term benefits of biochar and cow dung DOI Creative Commons

Hongyin Zhou,

Zhong Yu,

Shiying Zhang

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 28, 2025

Secondary salinization of soil seriously hinders the healthy cultivation facility grapes. Biochar has been shown to mitigate negative effects saline stress on plants. However, long-term response mechanism between soil’s key physicochemical properties, ion concentration, and enzyme activity physiological resistance grape plants biochar combined with cow dung application alleviate secondary remains unclear. In this study, a field experiment was set up once in September 2021 five different treatments, including no amendments. which used as blank control (CK), (10 t·ha -1 , T1), (30 T2), mixed (5 +15 T3), +30 T4), respectively. The results showed that compared CK treatment, treatments significantly reduced total salt(TS) content electrical conductivity(EC) value; increased water-stable aggregates nutrient content; stimulated an increase urease (S-UE), sucrose (S-SC) phosphatase(S-ALP)activities; changed exchangeable calcium magnesium concentrations. Among T4 treatment TS EC by 73.03% 61.11%, chlorophyll malondialdehyde (MDA), activities superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) leaves. decreased MDA, SOD, POD, CAT 54.59%, 40.14%, 44.28%, 70.17% Correlation analysis balance ions stability aggregate structure were factors alleviating stress. conclusion, can oxidative improve quality grapes improving aggregates, coordinating concentration ions, stimulating activity.

Language: Английский

Citations

0

An extensive pesticide residue study in minor Polish vegetables based on critical consumer diets DOI
Ewa Rutkowska, Piotr Kaczyński, Piotr Iwaniuk

et al.

Food Control, Journal Year: 2025, Volume and Issue: unknown, P. 111383 - 111383

Published: April 1, 2025

Language: Английский

Citations

0

Genome-Wide Identification and Expression Profiling of Potato (Solanum tuberosum L.) Universal Stress Proteins Reveal Essential Roles in Mechanical Damage and Deoxynivalenol Stress DOI Open Access
Tianshuai Qi,

Fumeng He,

Xinqi Zhang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 1341 - 1341

Published: Jan. 22, 2024

Universal stress proteins (USPs) play an important regulatory role in responses to abiotic stress. Most of the research related USPs so far has been conducted on plant models such as Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa L.), and cotton (Gossypium hirsutum L.). The potato (Solanum tuberosum L.) is one four major food crops world. susceptible mechanical damage infection by pathogenic fungi during transport storage. Deoxynivalenol (DON) released Fusarium can seriously degrade quality potatoes. As a result, it great significance study expression pattern StUSP gene family under conditions. In this study, total 108 USP genes were identified from genome Atlantic potato, divided into subgroups. Based their genetic structure, physical chemical properties other aspects biological characteristics are comprehensively analyzed. Collinear analysis showed that homologous StUSPs representative species lycopersicum, Arabidopsis, Oryza L., Nicotiana attenuata) highly conserved. cis-regulatory elements promoter involved hormones, environmental stress, damage, light response. RNA-seq there differences patterns members each subgroup different stresses. A Weighted Gene Coexpression Network Analysis (WGCNA) central differential coexpression mainly plant–pathogen response process, hormone signal transduction, biosynthesis process secondary metabolites. Through qRT-PCR analysis, was confirmed StUSP13, StUSP14, StUSP15, StUSP41 may be candidate adversity results provide basis for further functional potatoes

Language: Английский

Citations

3