Efeito de diferentes posições de cabeça e pescoço nas variáveis cinemáticas do Mangalarga Marchador DOI Open Access

Natália Almeida Martins

Published: Feb. 25, 2022

O Mangalarga Marchador é uma raça de cavalo conhecida pelo conforto seu andamento natural, que apesar muito apreciado, ainda faltam dados científicos para melhor compreender os fatores interferem na marcha. Neste estudo, objetiva- se avaliar as alterações cinemática marcha do em função diferentes posições cabeça e pescoço durante a Marcha batida, especificamente; porcentagem dissociação, o comprimento passada trajetória linear cada eixo compõe movimento tridimensional. Foram usadas dezenove câmeras da OptiTrack Prime 17W (360 Hz), sistema captura tridimensional, distribuídas um galpão, permitindo dos marcadores cascos fossem capturados simultaneamente velocidade média 9,39 km/h nas “Ideal”, “Invertida”, “Livre” “Vertical”. Após coleta tridimensionais ruído branco foi eliminado numéricos foram obtidos subsequentemente analisados no Motive MTV- BDY2, OptiTrack. A posição interfere sendo “Invertida” tem maior dissociação dentre avaliadas, “Ideal” “Vertical” menores menor dissociação. Comprimento apresentou enquanto demais obtiveram médias iguais. Os gestos variaram formato das curvas vertical. Todas variáveis cinemáticas variações entre avaliadas neste estudo. Palavras-chave: Biomecânica. Marcha. Cinemática.

A New Approach of Sperm Motility Subpopulation Structure in Donkey and Horse DOI Creative Commons
Sabrina Gacem, Anthony Valverde, Jaime Catalán

et al.

Frontiers in Veterinary Science, Journal Year: 2021, Volume and Issue: 8

Published: May 25, 2021

This study aimed to characterize the sperm kinematic values with high frames per second, define subpopulation structure of a horse and donkey compare them. A total 57 fresh semen ejaculates (26 Spanish 16 Arabian breeds 10 donkeys) were collected subsequently analyzed for parameters using Computer-aided motility analysis ISAS®v1.2 system Spermtrack® 10-μm depth counting chamber. Sequences recorded at 250 eight automatically evaluated. All showed significant differences between breeds. evaluated excellent characteristics, significantly higher all donkeys compared horses except beat-cross frequency. Donkey was faster linear than horse. Regarding differences, had average path velocity, curvilinear frequency rapid, but Arab more linear. The principal component three subpopulations in ejaculate different characteristic dominant both straight, beat (38.2 41.7%, respectively), whereas lowest slowest non-linear sperms. This, plus slight distribution these horses, found. In conclusion, permitted have new interpretation motile species breed differences. More so, future works on spermatozoa should take into account that may interfere alter real performed.

Language: Английский

Citations

14
Advances in Donkey and Mule Research DOI Creative Commons
Ana Martins‐Bessa, Amy McLean

Animals, Journal Year: 2024, Volume and Issue: 14(15), P. 2238 - 2238

Published: Aug. 1, 2024

Donkeys (

Language: Английский

Citations

1
Addition of Reduced Glutathione (GSH) to Freezing Medium Reduces Intracellular ROS Levels in Donkey Sperm DOI Creative Commons
Iván Yánez-Ortiz, Jaime Catalán, Ariadna Delgado‐Bermúdez

et al.

Veterinary Sciences, Journal Year: 2021, Volume and Issue: 8(12), P. 302 - 302

Published: Dec. 2, 2021

In donkeys, the use of frozen-thawed sperm for artificial insemination (AI) leads to low fertility rates. Furthermore, donkey produce a large amount reactive oxygen species (ROS), and post-AI inflammation induces formation neutrophil extracellular traps (NETosis), which further generates many more ROS. These high ROS levels may induce lipid peroxidation in plasma membrane, thus affecting its integrity. Enzymatic non-enzymatic antioxidants, mainly found seminal (SP), are responsible maintaining redox balance. However, this fluid is removed prior cryopreservation, thereby exposing cells oxidative stress. The exogenous addition antioxidants freezing medium can reduce detrimental effects caused by generation. Therefore, aim study was evaluate how different reduced glutathione (GSH) concentrations (control, 2 mM, 4 6 8 10 mM) fresh affect their cryotolerance. Total progressive motility, kinematic parameters motile subpopulations were significantly (p < 0.05) from control treatments containing mM GSH, but not at lower concentrations. Plasma acrosome membrane integrity, mitochondrial potential (MMP) intracellular superoxide (O2-) affected > any GSH concentration. Interestingly, however, or percentages viable with overall compared control. conclusion, able tolerate concentrations, differs what has been observed other species. This antioxidant capacity suggests that could be important during impact using like improve resilience freeze-thawing limited

Language: Английский

Citations

9
Evaluation of Testicular Volume and Correlation with Sperm Production in Martina Franca Donkeys: A Parameter to Consider When Approving Breeding Jacks DOI Creative Commons
Roberta Bucci,

Ippolito De Amicis,

Salvatore Parrillo

et al.

Animals, Journal Year: 2023, Volume and Issue: 13(23), P. 3619 - 3619

Published: Nov. 23, 2023

Good sperm production is a notable requirement for subjects intended reproduction, particularly in endangered species, and it has been demonstrated that horse stallions, this correlated to testicular volume. The present study, which involved Martina Franca jacks, aimed determine whether, also breed, there correlation between the total number (TSN) Testes were measured with both ultrasound caliper. Testicular volume was calculated using two different formulas: one representing of an ellipsoid developed describe round-shaped testicles. average concentration 380.14 ± 254.58 × 106/mL, while TSN 16.34 7.76 109. Our findings evidenced significant (r > 0.75; p < 0.05) only formula V (cm3) = 33.57 H − 56.57 testes. Moreover, significance data obtained (VTs-us 315.03 25.83 cm3) but not In conclusion, can be suggestive good fertility jacks; thus, parameter could considered when selecting breeding animals.

Language: Английский

Citations

3
Optimization of human semen analysis using CASA-Mot technology DOI

Almudena García‐Molina,

Nuria Navarro,

Anthony Valverde

et al.

Systems Biology in Reproductive Medicine, Journal Year: 2023, Volume and Issue: 69(2), P. 166 - 174

Published: Feb. 20, 2023

The purpose of this study is to investigate the optimal framerate (FR) and use different counting chambers for improving CASA-Mot technology in Andrology. Images were captured at 500 fps, then segmented analyzed several ranges FRs (from 25 250) define asymptotic point that as an FR. This work was replicated using based capillarity (disposable) or drop displacement (reusable) their effects on motility results kinematic values samples under experimental conditions. α value (asymptote corresponding FRo) exponential curve 150.23 a VCL 130.58 mm/s, far from 98.89 mm/s 50 fps (the highest FR used by most current systems). Our have shown that, when reusable chambers, type depth influence. In addition, obtained depending area image inside chamber types. To reliable human sperm studies, almost 150 should be capturing analyzing differences between considered sampling areas, obtain representative whole sample.

Language: Английский

Citations

1
Role of Seminal Plasma in the Equine Reproduction DOI
Jordi Miró, Jaime Catalán

Published: Jan. 1, 2024

Language: Английский

Citations

0
Condiciones óptimas del análisis CASA-Mot del semen de verraco: efecto de la tasa de fotogramas para diferentes cámaras y campos de recuento espermático DOI Open Access
Vinicio Barquero, Francisco Sevilla, Josué Calderón‐Calderón

et al.

Revista de Investigaciones Veterinarias del Perú, Journal Year: 2021, Volume and Issue: 32(5), P. e19832 - e19832

Published: Oct. 27, 2021

El objetivo del estudio fue determinar el efecto de la tasa fotogramas sobre diferentes profundidades cámara recuento espermático en semen verraco y analizar distribución los espermatozoides campos longitudinales conteo. Se colectó una muestra a 10 verracos Pietrain 2-3 años. determinaron ocho parámetros cinética espermática mediante sistema CASA (Computer-assisted analysis)-Mot (ISAS®v1). utilizaron cámaras ISAS®D4C con 10, 16 20 μm frecuencia captura imagen 25, 40 60 por segundo (fps). La profundidad afectó las variables espermática, obteniéndose valores más altos µm. (FR) presentó relación positiva fps, excepto para amplitud desplazamiento lateral cabeza (ALH) que un comportamiento inverso. variable sensible al cambio FR velocidad curvilínea (VCL), donde mejor nivel se observó 80 fps. disminución progresiva patrones cinemáticos conforme avanzaban cámaras. concluye uso incremento número total fps genera cambios significativos estimación verraco.

Citations

3
Efeito de diferentes posições de cabeça e pescoço nas variáveis cinemáticas do Mangalarga Marchador DOI Open Access

Natália Almeida Martins

Published: Feb. 25, 2022

O Mangalarga Marchador é uma raça de cavalo conhecida pelo conforto seu andamento natural, que apesar muito apreciado, ainda faltam dados científicos para melhor compreender os fatores interferem na marcha. Neste estudo, objetiva- se avaliar as alterações cinemática marcha do em função diferentes posições cabeça e pescoço durante a Marcha batida, especificamente; porcentagem dissociação, o comprimento passada trajetória linear cada eixo compõe movimento tridimensional. Foram usadas dezenove câmeras da OptiTrack Prime 17W (360 Hz), sistema captura tridimensional, distribuídas um galpão, permitindo dos marcadores cascos fossem capturados simultaneamente velocidade média 9,39 km/h nas “Ideal”, “Invertida”, “Livre” “Vertical”. Após coleta tridimensionais ruído branco foi eliminado numéricos foram obtidos subsequentemente analisados no Motive MTV- BDY2, OptiTrack. A posição interfere sendo “Invertida” tem maior dissociação dentre avaliadas, “Ideal” “Vertical” menores menor dissociação. Comprimento apresentou enquanto demais obtiveram médias iguais. Os gestos variaram formato das curvas vertical. Todas variáveis cinemáticas variações entre avaliadas neste estudo. Palavras-chave: Biomecânica. Marcha. Cinemática.

Citations

0