Standardisation of Dog Sperm Morphology Classification

Reproduction in Domestic Animals, Journal Year: 2025, Volume and Issue: 60(2)

Published: Feb. 1, 2025

ABSTRACT Sperm morphology is an important component of dog fertility analysis. Visual examination the gold standard technique for evaluation, but large variations within and across evaluators have been documented. Databases sperm images are valuable practical aids training performance testing can improve classification standardisation; however, such databases only useful when material expertly classified to serve as a reference. The objective this study was build annotated database generate illustrated guide with examples different presentations promote standardisation in classification. Semen samples ( n = 42) were obtained either from dogs presented evaluation or semen doses used artificial insemination. For image acquisition, wet preparations prepared evaluated using phase‐contrast optics under 1000× magnification. Selected cropped so that one included each file, 8817 created. by three veterinarians, specialists animal reproduction (Diplomates American College Theriogenologists) according criteria. Agreement among criterion create reconciled Complete agreement on defect category morphological observed 70.2% images, at least two veterinarians agreed another 27.4% images; therefore, based consensus 97.5% images. percentage morphologically normal 57%, primary involving head, midpiece, tail 18%, 20%, 5% cases, respectively. Among defective sperm, 39% had single 4% multiple defects. When total prevalence specific defects computed, contained 18% head abnormalities, 22% midpiece 7% abnormalities. Comprehensive illustrations variation abnormal built expert provided

Language: Английский

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Collection, preservation, and assessment of canine epididymal semen – current knowledge and future challenges

Annals of Animal Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 15, 2025

Abstract Epididymal spermatozoa have great potential as an alternative source of canine germ cells for the preservation unique features both endangered canid species and purebred dogs. semen collected from exceptional individuals is usually cryopreserved constitutes final reserve desired biological material. Cryopreservation recovered epididymis poses difficulties related to obtaining samples at sufficient concentrations, in addition its lower cryotolerance than ejaculated semen. Meeting challenges associated with conservation epididymal sperm necessitates delving deeper into available methods acquisition preservation, applying appropriate evaluation methods. The quality can be influenced by a number factors, collection technique applied insemination process utilized. Furthermore, our review provides comparison effectiveness selected extenders their supplements highlights aspects that require improvements or further investigation. analyzed literature data show squeezing retrograde flushing minimizes tissue erythrocytes contamination. In order ensure best after thawing, storage 4-5°C should avoided longer than: 24 h 0.9% saline 5 days chilling extenders. We concluded egg yolk based Bovimix® are most efficient cryopreservation sperm, however use supplementation such antioxidants requires

Language: Английский

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The Distribution of Boars Spermatozoa in Morphometrically Distinct Subpopulations after In Vitro Exposure to Radiofrequency Electromagnetic Radiation at 2500 MHz and Their Motility

Animals, Journal Year: 2024, Volume and Issue: 14(6), P. 828 - 828

Published: March 7, 2024

Anthropogenic radiofrequency electromagnetic radiation (RF-EMR) from wireless technologies has increased dramatically. The boar semen used for artificial insemination is essential in sustaining the pig industry, and additionally it also exposed to effects of RF-EMR technologies. Furthermore, there are no data on quality, this first analysis sperm’s morphometric parameters assessing effect spermatozoa subpopulations boars. This study investigated vitro breeding motility proportions according their head tail parameters. samples 12 boars were divided into control experimental groups. group a gigahertz transverse chamber at frequency 2500 MHz (the band 5G technology) an electric field strength 10 Vm−1 two hours. After exposure, was evaluated both A smears performed using SFORM software (Version 1.0; VAMS, Zagreb, Croatia). progressive significantly reduced (74.7% vs. 85.7%). PC cluster revealed subpopulations: S1, with more regular shape smaller midpiece outline, S2, elongated larger outline. had greater proportion S1 subpopulation (68.2% 64.4%). resulted decreased lower higher fertilizing potential.

Language: Английский

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Modified diff-quick staining for canine sperm morphology

Clinical Theriogenology, Journal Year: 2024, Volume and Issue: 16

Published: Feb. 16, 2024

Sperm morphology assessment requires specialized microscopes or stains. Diff-quick (DQ) is considered a universal stain that cost-effective; however, morphological evaluation of sperm using DQ staining poor and not encouraging. Therefore, this study investigated modifications to the protocol improve identification defects dog compared modified techniques with eosin-nigrosin, Karras, differential interference contrast microscopy [DIC]). One ejaculate from each 9 dogs was used. To perform proposed (DQ1 DQ2), dried semen smears were fixed by immersing for 10 seconds in solution 1 5 minutes solutions II III kit. After third solution, slides DQ1 rinsed water whereas DQ2 but vertically supported facilitate drainage. Results suggested standard overestimated normal detached heads underestimated abnormal total DIC, DQ2. Acrosome abnormalities only detectable In conclusion, prolonging exposure enhanced sensitivity evaluation, avoiding rinse as final step improved visualization certain acrosome sperm. can serve viable alternative clinical practice.

Language: Английский

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Morphometry of Boar Spermatozoa in Semen Stored at 17 °C—The Influence of the Staining Technique

Animals, Journal Year: 2022, Volume and Issue: 12(15), P. 1888 - 1888

Published: July 25, 2022

The aim of the study was to assess morphometry sperm during storage liquid boar semen at 17 °C. An attempt also made evaluate suitability three staining methods for assessment morphometry. carried out on 20 Landrace boars. Semen collected from boars every 5 days by manual method. Four ejaculates each were analysed (80 in total). Analyses performed five times: 1 h, 24 48 96 and 168 h after collection. Blisters with insemination doses opened immediately before analyses. From dose, smears prepared morphometric evaluation sperm, which stained (eosin-nigrosin-EN, eosin-gentian-EG, SpermBlue-SB). Morphometric measurements 15 randomly selected normal morphology slide. included following parameters: head length, width, area, perimeter; tail length; total length. results used calculate shape index. dimensions shown change extent these changes, however, depended method used, as result different case both tail. In slides eosin-nigrosin method, smaller time than SpermBlue eosin-gentian methods.

Language: Английский

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Diagnostic tests in canine andrology - What do they really tell us about fertility?

Theriogenology, Journal Year: 2022, Volume and Issue: 196, P. 150 - 156

Published: Nov. 12, 2022

Dog breeders often require breeding soundness evaluations which include andrological examinations of the genital organs, hormone measurements, and semen analyses. During past decades, a considerable number research results have been published, allowing diagnoses specific conditions fertility assessment. For examinations, however, no standard procedures defined for some parameters different reference ranges published. Therefore, examination from facilities are difficult to compare profound conclusions regarding health male dog not always possible. Conventional examination, is still useful in identifying deviations or normality, especially if confounding factors taken into account exam repeated case inconclusive findings. A standardization would help harmonize exchange interpretation

Language: Английский

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Kinematic and Morphometric Assessment of Fresh Semen, before, during and after Mating Period in Brahman Bulls

Animals, Journal Year: 2023, Volume and Issue: 14(1), P. 132 - 132

Published: Dec. 29, 2023

The objective of the present study was to determine effects that reproductive season has on motility, kinematics, morphology, and sperm morphometry Brahman bulls evaluated with a commercial CASA system. experiment carried out at Costa Rica Institute Technology from March August 2021. A total eight were used. 28 ejaculates collected in pre-mating period (PMP), during it (DMP), after (AMP) using an electroejaculator. concentration measured Accuread photometer. motility Spermtrack® counting chamber. analyses performed CASA-Mot ISAS®v1 morphology analyzed microscope negative phase contrast objective. Morphometry CASA-Morph. did not differences between PMP AMP; however, significantly higher than DMP (p > 0.05). Regarding progressiveness variables, linearity forward progression (LIN), straightness (STR), wobble (WOB) < 0.05) DMP. kinematic principal component analysis grouped all variables into three factors effect found parameters head middle part sperm, such as width perimeter, which greater PMP. length show differences; both larger AMP. insertion distance piece Finally, contained cells angle These findings are important understand implications status quality consider them andrological evaluations.

Language: Английский

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Epididymal sperm characteristics in Brown-throated Sloth (Bradypus variegatus Schinz, 1825)

Theriogenology Wild, Journal Year: 2024, Volume and Issue: 5, P. 100113 - 100113

Published: Jan. 1, 2024

Language: Английский

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Short Communication: Characteristics of the endangered Javan banteng (Bos javanicus) spermatozoa

Biodiversitas Journal of Biological Diversity, Journal Year: 2023, Volume and Issue: 24(2)

Published: Feb. 16, 2023

Abstract. Yoelinda VT, Arifiantini RI, Solihin DD, Agil M, Setiadi DR, Hastuti YT, Manansang J, Sajuthi D. 2023. Short Communication: Characteristics of the endangered Javan banteng (Bos javanicus) spermatozoa. Biodiversitas 24: 759-766. Morphology and morphometry spermatozoa become essential fertility parameters in domestic wild animals. Such morphological abnormalities can arise from genetic or environmental origins cause deprivation. Semen collection using transrectal massage has been used several animals is known to be simpler, safer, less painful than artificial vaginas electro-ejaculation. This study aimed evaluate morphometrical features head. The objective this was also, use as a semen protocol. conducted Taman Safari Indonesia, Cisarua, Bogor, West Java, involving one bull. samples were collected weekly, smeared, stained with modified Williams' staining method. found ten types head abnormality, comprising major defects, origin. There also nine tail teratoid form abnormalities. total normal proportion 54.30±1.74%. Morphometric dimensions length, width, area 9.88±0.01 µm, 4.91 38.12±0.05 µm2, respectively, length 60.48±0.03 ?m. Although our result limited by number available for study, first report on bull's sperm morphology still need considered. possibility protocol demonstrated may helpful support conservation approach terms breeding program strategy.

Language: Английский

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Feline sperm head morphometry in relation to male pedigree and fertility

Theriogenology, Journal Year: 2023, Volume and Issue: 208, P. 119 - 125

Published: June 7, 2023

Computer-assisted sperm morphometry analysis is an advanced tool which allows to precise measure head parameters like length, width, area, and perimeter. On the basis of these calculated parameters, morphometric subpopulations spermatozoa can be distinguished. In many species, distribution subpopulation within ejaculate related male fertility. There no information about such a relation for domestic cats; therefore, aim this study was evaluate whether from non-pedigree purebred cats differ in parameters. The second check if there relationship between Urethral semen collected 27 tomcats, divided into three groups: unknown fertility, infertile fertile cats. assessment performed by CASMA, followed principal component clustering. results revealed huge intra- inter-individual variation sperm-head were identified feline semen. Neither mean values nor fertility We hypothesize that other factors, especially abnormalities midpiece tail, overall worse quality males could have masked effect subtle changes morphometry.

Language: Английский

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