Animals, Journal Year: 2022, Volume and Issue: 12(13), P. 1632 - 1632
Published: June 25, 2022
The aim of this study was to identify the differentially expressed genes (DEG) from skeletal muscle and liver samples animal models for metabolic diseases in humans. To perform study, fatty acid (FA) profile RNA sequencing (RNA-Seq) data 35 tissue (SOY1.5,
Language: Английский
Biomolecules, Journal Year: 2025, Volume and Issue: 15(1), P. 134 - 134
Published: Jan. 15, 2025
Excessive abdominal fat deposition in chickens disadvantages feed conversion, meat production, and reproductive performance. Intramuscular contributes to texture, tenderness, flavor, serving as a vital indicator of overall quality. Therefore, comprehensive analysis the regulatory mechanisms governing differential versus intramuscular is essential breeding higher-quality with ideal distribution. This review systematically summarizes underlying traits at chromatin, genomic, transcriptional, post-transcriptional, translational, epigenetic-modification scales. Additionally, we summarize role non-coding RNAs protein-coding genes deposition. These insights provide valuable theoretical foundation for genetic engineering high-quality high-yielding chicken breeds.
Language: Английский
Citations
1
Animals, Journal Year: 2025, Volume and Issue: 15(3), P. 422 - 422
Published: Feb. 3, 2025
Traditional pig breeding has improved production traits but faces limitations in genetic diversity, disease resistance, and environmental adaptation. Gene editing technologies, such as CRISPR/Cas9, base editing, prime enable precise modifications, overcoming these expanding applications to biomedical research. Here, we reviewed the advancements gene technologies pigs explored pathways toward optimized swine genetics for a resilient adaptive livestock industry. This review synthesizes recent research on tools applied pigs, focusing CRISPR/Cas9 its derivatives. It examines their impact critical role human models. Significant have been made targeting genes those conferring immunity porcine reproductive respiratory syndrome viruses. Additionally, gene-edited are increasingly used models diseases, demonstrating technology’s broader applications. However, challenges off-target effects, ethical concerns, varying regulatory frameworks remain. holds substantial potential sustainable productive by enhancing key supporting Addressing technical through integrated approaches will be essential realize full potential, ensuring resilient, ethical, sector future generations
Language: Английский
Citations
1
Poultry Science, Journal Year: 2024, Volume and Issue: 103(4), P. 103524 - 103524
Published: Feb. 1, 2024
The objective of this study was to investigate the effects sex on meat quality and composition amino fatty acids in breast muscles White King pigeon squabs. Untargeted metabolomics also conducted distinguish metabolic plasma different sexes. Compared with male squabs, female squabs had greater intramuscular fat (IMF) deposition lower myofiber diameter hydroxyproline content, leading a shear force. Female higher monounsaturated acid n-6 n-3 polyunsaturated proportions muscle, lipogenesis capacity via upregulation PPARγ, FAS LPL gene expression. Moreover, inosine 5′-monophosphate, essential, free sweet-tasting contents. Furthermore, Spearman's correlations between differential metabolites key parameters were assessed, putrescine, N-acetylglutamic acid, phophatidylcholine (18:0/P-16:0) trimethylamine N-oxide found contribute quality. In summary, may have better nutritional value than that females, but it inferior terms sensory properties, which can be attributed IMF content force value. Our findings enhance our understanding variation squab quality, providing basis for future research breeding.
Language: Английский
Citations
6
Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11
Published: Feb. 16, 2024
Exogenous supplementation of guanidinoacetic acid can mechanistically regulate the energy distribution in muscle cells. This study aimed to investigate effects on liver and breast fat deposition, lipid levels, metabolism-related gene expression ducks. We randomly divided 480 42 days-old female Jiaji ducks into four groups with six replicates 20 for each replicate. The control group was fed basal diet, experimental were diet 400, 600, 800 mg/kg (GA400, GA600, GA800) acid, respectively. Compared group, (1) total cholesterol ( p = 0.0262), triglycerides 0.0357), malondialdehyde 0.0452) contents lower GA400, GA600 GA800 liver; (2) 0.0365), 0.0459), 0.0326) decreased GA800; (3) high density lipoprotein 0.0356) apolipoprotein-A1 0.0125) increased (4) 0.0489) higher (5) lipase 0.0325) (6) malate dehydrogenase 0.0269) (7) insulin induced 1 0.0326), fatty transport protein 0.0412), 0.0235) relative (8) 0.0269), 0.0234), 0.0425) muscle. In this study, optimum dosage 600 improved
Language: Английский
Citations
5
Food Science of Animal Resources, Journal Year: 2024, Volume and Issue: 44(4), P. 832 - 848
Published: March 12, 2024
This study was carried out to assess the quality properties, components associated with taste and aroma of beef as a function breed. For this purpose, steers from four Korean native cattle breeds: Hanwoo (n=10), Chikso black (n=12, BHW) Jeju JBC) were used. The all raised under identical conditions finished at similar age around 30-months old. Following 24 h slaughter, longissimus lumborum (LL) muscles collected used for analysis meat quality, fatty acids, flavor-related (metabolic compounds, free amino volatiles). presented significantly higher intramuscular fat content (IMF, 22.85%) than BHW (11.78%), (9.25%), JBC (9.14%) (p<0.05). breed showed lighter redder color, lower shear force value "healthier" acid profiles it had total unsaturated acids With regard components, also contents metabolites umami sweet tastes, fat-derived volatile compounds (aldehydes, alcohols, ketones) aroma. It may be concluded that there considerable difference in properties among breeds. variations IMF main factors contributing typical flavors
Language: Английский
Citations
5
Journal of Food Composition and Analysis, Journal Year: 2024, Volume and Issue: 134, P. 106535 - 106535
Published: Oct. 1, 2024
Language: Английский
Citations
5
Veterinary and Animal Science, Journal Year: 2022, Volume and Issue: 17, P. 100262 - 100262
Published: July 6, 2022
Noncommunicable diseases such as cardiovascular disease, obesity, diabetes, and cancer now outnumber all other health ailments in humans globally due to abrupt changes lifestyle following the industrial revolution. The revolution has also intensified livestock farming, resulting an increased demand for productivity stressed animals. industry faces significant challenges from a projected sharp increase global food high animal protein demand. Nutrition genomics holds great promise future its advances have opened up whole new world of disease understanding prevention. Nutrigenomics is study interactions between genes diet. It investigates molecular relationships nutrients identify how even minor modifications could potentially alter human health/performance by using techniques like proteomics, transcriptomics, metabolomics, lipidomics. Dietary mostly studied focus mainly on production traits through protein, fat, mineral, vitamin supplementation changes. meticulously selects fine-tuning expression that match animal/human genotypes better health, productivity, environment. As step forward, nutrigenomics integrates nutrition, biology, genomics, bioinformatics, medicine, epidemiology understand role epigenetic factor occurrence these diseases. This review aims provide comprehensive overview fundamental concepts, latest advances, studies field nutrigenomics, emphasizing interaction diet with gene expression, it relates along human-animal interphase.
Language: Английский
Citations
18
Metabolites, Journal Year: 2025, Volume and Issue: 15(4), P. 231 - 231
Published: March 28, 2025
Ectopic fat deposition refers to lipid accumulation that affects metabolic function and tissue characteristics. Japanese Black cattle are distinguished by their high intramuscular content, which contributes distinctive character. However, the genetic mechanisms underlying these traits remain unclear. This study compared gene expression patterns in different muscle regions identify genes associated with accumulation. First, we conducted RNA sequencing analyze differences profiles among sternocleidomastoid, pectoralis minor, major muscles. In addition, single-cell nuclear was investigate cellular composition of tissues. Distinct were observed pectoralis, contains a proportion fat, adipocyte-related such as FABP4, SCD, ADIPOQ highly expressed. lipases PNPLA2, LPL, MGLL, LIPE predominantly expressed whereas PLA2G12A, PLD3, ALOX15 specifically myofibers. Moreover, subclass fibro-adipogenic progenitor cells differentiate into adipocytes found express related microenvironment formation, including ICAM1, TGFBRs, members COL4A family. provides novel insight regulation It improves our understanding molecular meat
Language: Английский
Citations
0
Research in Veterinary Science, Journal Year: 2025, Volume and Issue: 189, P. 105646 - 105646
Published: April 6, 2025
The brown seaweed Laminaria digitata, known for its prebiotic qualities, and alginate lyase supplementation, may improve the growth development of piglets during critical post-weaning phase. purpose this study was to ascertain effects 10 % L. digitata 0.01 on proteome metabolome longissimus lumborum muscle in weaned piglets. Findings suggest that enzyme supplement has a marginal effect compared diet alone when control. increased prevalence proteins related contraction structure (such as ACTBL2), while it decreased presence glycolytic (like GPI ALDOC). It also abundance negative regulation insulin receptor pathways, such RABGAP1 TSC2. Conversely, associated with fatty acid oxidation (ALOXE3) calcium balance (WFS1), reflecting impacts dietary n-3 polyunsaturated acids lower diet. As metabolome, remained mostly unchanged by treatments, except mannitol threonine, which were enriched consequence inclusion.
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4440 - 4440
Published: May 7, 2025
The Slovenian autochthonous breed, Krškopolje pig, is known for high fatness and better adaptability to different environmental conditions feed resources. However, the metabolic processes underlying these adaptations, especially in response diets, have not yet been studied. A deeper understanding of mechanisms could provide valuable insights into breed’s conditions. Therefore, main objective this study was evaluate effect a low-protein (LP) diet on adipose tissue pigs reared either organic outdoor (n = 2 × 12) or conventional indoor 14) systems. In system, LP had no adipocyte size compared control (high-protein) diet, while it increased lipogenic enzyme activities monounsaturated fatty acid content, decreased polyunsaturated content (p < 0.05). RNA sequencing revealed upregulation 28 genes downregulation 37 genes. upregulated were mainly involved lipid metabolism (ACLY, FASN, ACACA, MOGAT2), oxidative stress, mitochondrial function. smaller adipocytes 0.05), whereas differences detected composition > 0.10). 30 downregulated heat shock proteins (HSP70.2, HSPA6) innate immune system (MSR1, TREM2, CSF3R) downregulated. To conclude, present showed that affected gene expression pigs, with transcriptomic responses observed rearing
Language: Английский
Citations
0