The wildlife biobanking of germ cells for in situ and ex situ conservation in Japan

Theriogenology Wild, Journal Year: 2024, Volume and Issue: 4, P. 100086 - 100086

Published: Jan. 1, 2024

Japan arcs from north to south and is abundant in biodiversity with different climates each region. has many endemic species/populations, such as the Tsushima leopard cat (Prionailurus bengalensis euptilurus) Okinawa rail (Hypotaenidia okinawae), which threaten become endangered. Furthermore, one of countries highest number zoos aquariums world keeps wildlife species, are endangered, captive environments. Effective management genetic diversity acceleration reproduction essential for conservation endangered both inside outside their natural habitats. Germ cell preservation assisted reproductive technologies have great potential rescue resources contribute species. The long-term germ cells reviewed here context maintenance animals that died environments or habitats Japan. Here, we present a review our cross-disciplinary strategy conserve species using resource genomic endocrine research.

Language: Английский

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Salpingo-peritonitis in captive African Houbara bustards ( Chlamydotis undulata )

Avian Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 29

Published: April 28, 2025

Captive breeding of endangered species, such as the African Houbara bustard (Chalmydotis undulata) is an essential part conservation programs. However, several factors may negatively impact health and reproductive performance captive animals, including genital infections. Between 2010 2023, a total 439 cases salpingo-peritonitis were diagnosed in flock breeders belonging to Moroccan project, relying exclusively on artificial insemination incubation eggs (annual prevalence from 0,07%, 1.64%). To improve overall knowledge pathology this avian representative sample 14 registered 2022 characterized, combining gross pathology, histopathology, microbiology molecular biology. Overall, 4/14 bustards died spontaneously exhibited septicemic changes, while 10/14 euthanized birds presented signs consistent with chronic oviductal impaction. The latter was further confirmed at histopathology and, 8 bustards, associated mucosal changes resembling cystic endometrial hyperplasia, condition commonly reported mammals potentially predisposing secondary bacterial Escherichia coli isolated tract 12/14 birds. Molecular characterization nine selected isolates, revealed presence virulence pathogenic E. coli. variety virulence, well antimicrobial sensitivity profiles identified, combined pathological presentation, suggested involvement multiple strains. addition specific genes, management host-related should also be consider important determinants potential Houbaras.

Language: Английский

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Sperm Cryopreservation in Canaries to Protect Endangered Songbird Species: Comparison of Different Cryoprotectants

Veterinary Medicine and Science, Journal Year: 2024, Volume and Issue: 10(6)

Published: Oct. 30, 2024

ABSTRACT Sperm cryopreservation is a rather complex process that needs to be adapted wild and domestic bird species ensure adequate efficiency. This study aimed determine the usability of different cryoprotectants in Gloster canary sperm. For this purpose, sperm samples were collected from 12 2‐year‐old male canaries three times week using cloacal massage for 4 weeks. After individual evaluation, combined. Mixed divided into two groups study. Overall, 8% dimethyl sulfoxide (DMSO) ethylene glycol (EG) used as cryoprotectants. drawn straws after adding Dulbecco's Modified Eagle Medium (DMEM) extender with high glucose ratio 1:1 frozen −80°C liquid nitrogen vapour then stored at −196°C. Frozen‐thawed semen evaluated regarding motility, vitality, plasma membrane integrity (hypoosmotic swelling test [HOST]), density abnormal spermatozoa rate. The highest motility value freezing thawing was determined EG group 31.667% ± 4.773%. In addition, normal morphology statistically significantly higher EG‐frozen group, whereas head tail abnormality low ( p < 0.05). DMEM containing more advantageous than DMSO spermatological parameters could long‐term storage

Language: Английский

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Inseminação artificial em aves não-domésticas: Uma revisão

Research Society and Development, Journal Year: 2023, Volume and Issue: 12(7), P. e10712742588 - e10712742588

Published: July 21, 2023

Atualmente existem no Brasil 257 espécies de aves, conhecidas classificadas como “ameaçadas extinção”. Na tentativa tentar solucionar esse problema, vários programas criação e propagação aves em cativeiro, o do mutum Alagoas (Pauxi mitu), a ararinha-azul (Cyanopsitta spixii), entre outros vem sendo realizados nas últimas décadas, com intuito restabelecer manutenção da diversidade genética restaurar populações selvagens dessas ex situ para posteriormente devolvê-las seu ambiente natural. No entanto, reprodução possui uma série entraves inerentes à manejo pequenas fechadas ameaçadas, incluindo lidar instabilidade demográfica, física deficiências comportamentais, incompatibilidade sexual, falta sincronia necessidade manter genética. Diante dessa situação, biotecnia inseminação artificial (IA) tem potencial contribuir ajudar parte desses problemas. Neste contexto, esta revisão objetivo apresentar discutir uso IA não-domésticas, auxiliar na conservação ameaçadas divulgar criadores, técnicos profissionais que atuam aves.

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