Developmental programming of reproduction in the female animal

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 263, P. 107456 - 107456

Published: March 16, 2024

Language: Английский

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Sequencing and culture-based characterization of the vaginal and uterine microbiota in beef cattle that became pregnant or remained open following artificial insemination

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(6)

Published: Nov. 3, 2023

Emerging evidence suggests that microbiome-targeted approaches may provide a novel opportunity to reduce the incidence of reproductive failures in cattle. To develop such microbiome-based strategies, one first logical steps is identify microbiome features related fertility and isolate fertility-associated microbial species for developing future bacterial consortium could be administered before breeding enhance pregnancy outcomes. Here, we characterized vaginal uterine microbiota beef cattle became pregnant or remained open via artificial insemination identified associated with fertility. We compared similarities between heifers cows. Using culturing, provided new insights into culturable fraction their antimicrobial resistance. Overall, our findings will serve as an important basis research aimed at harnessing improved

Language: Английский

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Temporal changes in ewe vaginal microbiota throughout gestation

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 15, 2024

Introduction Numerous factors are known to influence reproductive efficiency in ewes, but few studies have investigated the potential role of vaginal microbiota sheep success. The objective this study was thoroughly characterize ewe throughout course pregnancy. Methods Vaginal samples were collected from 31 pregnant Hampshire and X Suffolk crossbred ewes on a weekly basis pre-breeding pregnancy testing then biweekly until just after lambing. To microbial communities, DNA extracted 16S rRNA gene Illumina MiSeq amplicon sequencing performed. Results Discussion Alpha diversity metrics indicated an increase species richness, evenness, overall gestation. Distinct shifts bacterial communities observed during gestation segregated into three periods: early gestation, transitional period mid/late During Actinobacillus , Histophilus, unclassified Leptotrichiaceae found greater relative abundance. period, population shift occurred characterized by increasing abundance Streptococcus Staphylococcus . Streptococcus, Ureaplasma had greatest These ewe’s likely related hormonal changes triggered growing conceptus, specifically blood concentration progesterone. potentially aligns with placental take-over progesterone production corpus luteum at approximately day 50 conception (gestational week 7). Understanding variability will allow for future comparison that did not become or abnormal pregnancies, which could lead discovery biomarkers outcome; understanding also development probiotics improve

Language: Английский

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Identification of microbial communities and multi-species biofilms contamination in seafood processing environments with different hygiene conditions

Food Microbiology, Journal Year: 2024, Volume and Issue: 122, P. 104553 - 104553

Published: April 25, 2024

Language: Английский

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Ammonia and hydrogen sulfide - new insights into gut microbiota and male infertility through meta-analysis

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 14

Published: Jan. 6, 2025

Ammonia (NH3) and hydrogen sulfide (H2S) are produced during digestion in the human gut, yet impact of these internally generated gases on male reproduction have received limited attention scientific research. We systematically reviewed 935 publications, spanning from 1947 to 2023, focusing external or internal NH3 and/or H2S, infertility, gut microbiota. Meta-analysis was conducted evaluate summary relative risk (RR) 95% confidence intervals (CIs) combined studies. Our findings revealed that H2S were negatively related Lactobacillus, which is beneficial fertility, whereas positively Bacteroides, showed negative effects fertility. The meta-analysis comparing Lactobacillus Bacteroides levels with statistically significant results (p<0.001). first confirm facts explored potential existence a microbiota-inner gases-male fertility axis gut.

Language: Английский

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Combined analysis of 16S rRNA gene sequencing data reveals core vaginal bacteria across livestock species

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 10, 2025

Investigating the livestock vaginal microbiota is of increasing interest due to its relationship with animal reproductive performance. Recent publications have uncovered a high degree variability microbiota, making it difficult focus functional research on individual microorganisms. To address this variability, we conducted combined analysis publicly available 16S rRNA gene amplicon sequencing datasets reveal core in cattle, sheep, and pigs. The goal was identify bacterial genera that were shared despite diverse overall sample population. A total 2,911 samples (715 964 1,232 pigs) from 29 different used analysis. Beta diversity revealed structural differences between species. Compositionally, most abundant phyla Bacillota , Pseudomonadota Bacteroidota. At genus level, an unclassified Pasteurellaceae genus, Ureaplasma Streptococcus abundant. Across species, compositional observed. cattle sheep contained higher abundance Histophilus whereas pig more Fusobacterium Parvimonas than other samples. Among 120 OTUs 82 present 70% same threshold, had 40 63 genera, while 22 50 genera. There 19 overlapping across three largely species-specific, although there eight OTUs. These included (OTU 21), Clostridium sensu stricto 1 18), Corynebacterium 6), which also some members microbiota. better understanding required for future studies aimed at elucidation significance microbes respect efficiency. identified will help guide mechanisms/pathways through organisms enhance or impede

Language: Английский

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Connecting microbial ecology to human fertility and reproduction: perspectives from the reproductive microbiomes of animals

F&S Reports, Journal Year: 2025, Volume and Issue: 6, P. 45 - 49

Published: April 1, 2025

Language: Английский

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Microbiome: Mammalian milk microbiomes: sources of diversity, potential functions, and future research directions

Reproduction and Fertility, Journal Year: 2024, Volume and Issue: 5(2)

Published: March 18, 2024

Graphical abstract Abstract Milk is an ancient, fundamental mammalian adaptation that provides nutrition and biochemical communication to offspring. Microbiomes have been detected in milk of all species studied date. In this review, we discuss: (a) routes by which microbes may enter milk; (b) evidence for proposed microbiome adaptive functions; (c) variation microbiomes across mammals; (d) future research directions, including suggestions how address outstanding questions on the viability functionality microbiomes. be sourced from maternal gastrointestinal tract, oral, skin, mammary gland neonatal oral skin Given variety microbial sources, stochastic processes strongly influence assembly, but appear influenced evolutionary history, diet, environment, nutrients. colonize intestinal tract produce gene metabolic products physiology, metabolism, immune system development. Limited epidemiological data indicate early-life exposure can result positive, long-term health outcomes. modified dietary changes providing mother with probiotics prebiotics. replacers (i.e. infant formula) benefit supplementation prebiotics, are lacking probiotics’ usefulness, should based. Overall, literature outside human model systems scarce. We highlight need mechanistic studies paired comparative mammals further our understanding evolution. A broader study has potential inform animal care relevance ex situ endangered species. Lay summary ancient supports growth development neonates infants. Beyond its nutritional function, influences aspects development, especially function. All kinds milks so far contained a microbiome. focus what known about collection bacterial members found include they nutrients, as well random chance. Once neonate begins nursing, their gut byproducts Empirical humans sparse. Greater will expand evolution improve animals under care.

Language: Английский

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Vaginal and Uterine Microbiota of Healthy Maiden Mares during Estrus

Veterinary Sciences, Journal Year: 2024, Volume and Issue: 11(7), P. 323 - 323

Published: July 18, 2024

This descriptive cross-sectional study compared the microbiota of uterus, vagina, clitoral fossa (CF), and perineal skin in healthy maiden mares during estrus. Twelve synchronized, (3–4 years old) from one single recipient mare herd were included. Microbial communities characterized by amplifying V3–V4 region 16S rRNA gene using Illumina MiSeq platform. The uterine vaginal had significantly lower richness (Chao-1) than (p < 0.05). vagina bacterial composition was similar presence abundance could be differentiated that CF skin. microbial (Jaccard Bray–Curtis distances) differed across body-site locations 0.05), which explained approximately 14% 19% variation for Jaccard distances, respectively. Firmicutes, Actinobacteria, Proteobacteria, Bacteroidetes dominant taxa uterus with higher proportions Proteobacteria samples to samples. Streptococcaceae Staphylococcaceae present high samples, while Lactobacillaceae not (<10%). We demonstrate estrus is but both distinct

Language: Английский

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Mammals’ sperm microbiome: current knowledge, challenges, and perspectives on metagenomics of seminal samples

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: April 17, 2023

Bacterial growth is highly detrimental to sperm quality and functionality. However, during the last few years, using sequencing techniques with a metagenomic approach, it has been possible deepen study of bacteria-sperm relationships describe non-culturable species synergistic antagonistic between different in mammalian animals. We compile recent metagenomics studies performed on semen samples provide updated evidence understand importance microbial communities results functionality males, looking for future perspectives how these technologies can collaborate development andrological knowledge.

Language: Английский

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