Effects of Phytase and Microalgae Supplementation on the Utilization of Aquafeeds for European Seabass (Dicentrarchus labrax) Formulated with a High Inclusion Level of Plant Protein

Aquaculture Research, Journal Year: 2024, Volume and Issue: 2024, P. 1 - 17

Published: May 6, 2024

Numerous studies have reported the disadvantages of using plant protein in aquafeeds owing to presence antinutritional factors. Particularly, phytate can be overcome by dietary supplementation with exogenous phytase. On other hand, use microalgae has been proven a valuable strategy for enhancing digestive functionality fish fed diets high content. It was hypothesized that simultaneous inclusion phytase useful improve growth performance and content protein. In this study, effects feeds supplemented different levels (FTU) 2.5% blend Arthrospira platensis Nannochloropsis gaditana were evaluated on performance, metabolism, gut European seabass (Dicentrarchus labrax) juveniles. Fish ad libitum 83 days five containing enzyme ratios (FTU), triplicate: (i) control (0 FTU); (ii) 500 FTU; (iii) 1,000 (iv) 2,000 (v) 10,000 FTU. At end feeding period, zootechnical indexes estimated, biological samples withdrawn physiological determinations. The results obtained showed an overall improvement productive efficiency, general intestinal highest (2,000 FTU). Final weight (75.51 ± 1.01 g vs. 66.76 1.26 g, p=0.005 ), SGR (1.15 0.02 1.00 0.02, p=0.006 ). feed efficiency (0.80 0.02% day−1 0.67 0.01% day−1, p=0.012 ) higher chemical composition muscle plasma metabolites did not vary among treatments, except glucose low glycogen liver FTU group. Focusing functionality, activities tended microalgae, significant differences found trypsin, chymotrypsin, alkaline phosphatase 1,000, 2,000, FTU, leucine aminopeptidase ( p<0.0001 Analysis morphology revealed combined increased villi height decreased diameter enterocyte height. at level seems lack influence parameters whereas only impacted growth, nutrient utilization, functionality. addition, faecal stripping final portion intestines, from which phosphorus nitrogen quantified. Apparent digestibility coefficient higher, 74.3% 77.8%, respectively, compared (52.4%). decrease amount P shed excreta animals phytase, fact evidenced absorption element. Overall, pointed out used 10,000, even combination as approach reducing costs boost fish, while minimizing environmental impacts juvenile farms.

Language: Английский

---

Local aquatic microflora as a potential source of probionts in biofloc technology for whiteleg shrimp, <i>Penaeus vannamei</i>

Environmental and Experimental Biology, Journal Year: 2025, Volume and Issue: 23(1), P. 1 - 8

Published: April 3, 2025

The Philippine shrimp aquaculture industry, a key supplier of Penaeus vannamei to domestic and international markets, faces significant disease challenges, particularly from pathogenic Vibrio species. Current management strategies often employ terrestrially-sourced probiotics, whose efficacy can be limited by environmental incompatibility with the aquatic host. This study aimed address this limitation isolating characterizing putative probiotic microorganisms local environment P. vannamei. Water samples, collected farm outlet pond in Negros Occidental, Philippines, were enriched brown sugar promote biofloc formation. Bacterial colonies isolated on Nutrient Agar supplemented 1% NaCl. In vitro antagonistic activity against pathogen harveyi was assessed via spot-on-lawn cross-streak assays. assay revealed 45% inhibition rate V. harveyi. Subsequent assays confirmed inhibitory effects five nine isolates. These isolates underwent morphological, biochemical, molecular characterization using 16S rRNA gene sequencing. Three identified as alginolyticus, while remaining two closely matched Pseudoalteromonas Given established use non-pathogenic species probiotics aquaculture, these findings highlight potential microflora source probionts for biofloc-based culture. approach may reduce reliance external sources, contributing enhanced industry sustainability.

Language: Английский

---

Effects of feeding European seabass (Dicentrarchus labrax) juveniles with crude, hydrolysed and fermented biomass of the invasive macroalga Rugulopteryx okamurae (Ochrophyta)

Aquaculture Reports, Journal Year: 2023, Volume and Issue: 34, P. 101877 - 101877

Published: Dec. 18, 2023

The increasing expansion of the invasive Asian alga Rugulopteryx okamurae along Andalusian coasts is a major challenge to marine biodiversity, and urgent coordinated measures are required for its removal or elimination. Among different actions, biotechnological valorisation could be strategy management alien biomass within frame Blue Economy. This work evaluates potential R. biomass, either raw processed by treatments based on enzymatic hydrolysis fermentation processes, used as dietary ingredient in juvenile seabass (Dicentrarchus labrax). These treatments, intended primarily improve nutrient availability, might also decrease content undesirable potentially harmful algal metabolites, such diterpenoid dilkamural. To this end, 90-day feeding trial was carried out using four experimental diets containing 5% (w/w) crude biomass. results obtained revealed that inclusion diet caused adverse effects fish growth, yielding lower values than those control group (p < 0.05), although detrimental effect negligible when algae prior feeds. In terms muscle composition, supplemented with induced an increase protein content, especially fed pre-treated Qualitative differences 0.05) were found fatty acid profile, significant ARA DHA contents algae-supplemented diets. A reduction 0.05)in liver lipid oxidation evidenced algae-enriched diets, treated Finally, negative gut functionality, these not observed previously processed. conclusion, functional sea bass, provided pre-treated, corroborated efficacy low-cost treatment method

Language: Английский

---

Potential Effects of Microalgae-Supplemented Diets on the Growth, Blood Parameters, and the Activity of the Intestinal Microbiota in Sparus aurata and Mugil cephalus

Fishes, Journal Year: 2023, Volume and Issue: 8(8), P. 409 - 409

Published: Aug. 9, 2023

This work aimed to assess the suitability of a microalgal blend as dietary ingredient for feeding juveniles marine carnivorous and herbivorous teleost, is case Sparus aurata Mugil cephalus, respectively, isolate microorganisms from different media characterize them on base their enzymatic activities antagonism against important fish pathogens. Thirty each species (70 ± 3.2 g S. mean weight 47 2.8 M. cephalus weight) were distributed in four tanks (15 individuals each) corresponding independent treatments (control microalgae diets designed species). Fish fed ad libitum 108 days. At end trial, weighed, plasma, liver, perivisceral fat, entire intestines obtained evaluation growth performance metabolic assessment. Furthermore, 117 bacterial strains isolated culture gastrointestinal tract further characterized potential use probiotics aquaculture. microalgae-supplemented diet (25% inclusion) showed significant increase gain, specific rate, feed efficiency, hepatosomatic, intestine length indices. However, somatic indices not affected by experimental diets. Plasma samples revealed higher levels glucose triglycerides decrease cortisol levels. No differences found any biochemical parameters among cephalus. In conclusion, both demonstrated favorable adaptation nutritional formulation employed this study, UMA-169 UMA-216 (both identified Bacillus pumilus) could be considered aquaculture they might benefit host health improving digestion absorption energy sources minimizing colonization pathogenic species.

Language: Английский

---

Affecting mechanism of Chlorella sorokiniana meal replacing fish meal on growth and immunity of Litopenaeus vannamei based on transcriptome analysis

Aquaculture Reports, Journal Year: 2023, Volume and Issue: 31, P. 101645 - 101645

Published: July 3, 2023

The purpose of this study was to analyze the affecting mechanism Chlorella sorokiniana meal replacing fish on growth performance and immune function Pacific white shrimp, Litopenaeus vannamei by transcriptomics analysis. In a control diet containing 560 g % /kg meal, chlorella used replace 0 %, 20 40 60 80 100 dietary form six iso-nitrogenous iso-lipidic diets (C0, C20, C40, C60, C80, C100). were fed shrimp (1.37 ± 0.02 g) for 8 weeks. Compared group, final body weight (FBW), specific rate (SGR) feed efficiency (FE) C20 group significantly improved (P < 0.05). There no significant difference in between C40 C0 > When replacement level ≥ FBW, SGR FE decreased analysis hepatopancreatic transcriptome, amount differentially expressed genes (DEGs) C80 C100 groups 322 (176 up 146 down), 482 (246 236 804 (487 317 1080 (684 396 down) 1262 (827 435 respectively, when compared group. GO Term KEGG pathway enrichment DEGs showed that enriched chitin binding inositol metabolism processes. Several immune-related signaling pathways also enriched, including C-type lectin receptor pathway, complement coagulation cascades, B-cell most these up-regulated lysosomal, protein digestion, absorption pathways. addition, groups, lipid catabolism process. high with affected utilization nutrients regulating related digestion absorption, aggravated body, thereby shrimp. might affect homeostasis cells reduce immunity through lysosomal pathway. summary, low (20 %) vannamei, which be realized activating regulate but substitution (≥ reduced

Language: Английский

---

Host development promotes the early microbiota succession of teleost surpassing dietary management

Aquaculture, Journal Year: 2024, Volume and Issue: 593, P. 741309 - 741309

Published: June 30, 2024

Language: Английский

---

Effects of water temperature on growth, health and digestive processes of the thicklip grey mullet Chelon labrosus

Aquaculture, Journal Year: 2024, Volume and Issue: 595, P. 741537 - 741537

Published: Aug. 30, 2024

This study aimed to identify the effects of water temperature on rearing omnivorous teleost Chelon labrosus and determine optimal for culture this interesting candidate diversification European aquaculture. The fish (initial weight 26.81 ± 6.2 g) were held at four temperatures (18, 22, 26 30 °C) in triplicate 90 days 100 L recirculating tanks ( n = 14 / tank). said assessed growth, intestinal health digestive processes fish. At 18 °C, energy consumption was low, leading a high accumulation reserves slow typical overwintering strategy. best growth results obtained 22 followed closely by which resulted low reserves. fact that °C grew less than ones while having similar consumption, suggested close end range, but no signs stress detected. However, had lower significantly less, can be considered indicative stress. Health impairment confirmed epithelial lesions found group. availability different groups is discussed light activities α-amylase, pepsin alkaline proteases. As an integration all these results, quadratic regression model resulting from values (SGR) relation performed, it allowed predicting actual 22.8 C. labrosus. first assessment , will highly valuable development its intensive culture. • Optimal °C. Slow reserve Some started proven impair animal welfare. No significant alterations flesh quality detected tested temperatures.

Language: Английский

---

Combined Dietary Administration of Chlorella fusca and Ethanol-Inactivated Vibrio proteolyticus Modulates Intestinal Microbiota and Gene Expression in Chelon labrosus

Animals, Journal Year: 2023, Volume and Issue: 13(21), P. 3325 - 3325

Published: Oct. 26, 2023

The use of functional feeds in aquaculture is currently increasing. This study aimed to assess the combined impact dietary green microalgae Chlorella fusca and ethanol-inactivated Vibrio proteolyticus DCF12.2 (CVP diet) on thick-lipped grey mullet (Chelon labrosus) juvenile fish. effects intestinal microbiota transcription genes related metabolism, stress, immune system were investigated after 90 days feeding. Additionally, fish challenged with Aeromonas hydrophila polyinosinic-polycytidylic acid (poly I:C) evaluate response. Microbiota analysis revealed no significant differences alpha beta diversity between anterior posterior sections fed control (CT) CVP diets. dominant genera varied groups; Pseudomonas Brevinema most abundant group, whereas Brevinema, Cetobacterium, predominant CT group. However, microbial functionality remained unaltered. Gene expression indicated notable changes hif3α, mhcII, abcb1, mx, tnfα different organs diet. In head kidney, gene variations observed following challenges A. or poly I:C, higher peak values seen injected I:C. Moreover, c3 mRNA levels significantly up-regulated group 72 h post-A. challenge. To conclude, incorporating C. V. labrosus diet affected species composition intestine while preserving its functionality. terms expression, effectively regulated stress immune-related genes, suggesting potential enhancement resistance against infections.

Language: Английский

---