The Effects of Mixed Inoculum Storage Time on In Vitro Rumen Fermentation Characteristics, Microbial Diversity, and Community Composition DOI Creative Commons
Chang Liu,

Jing Ge,

Jiaqi Dai

et al.

Animals, Journal Year: 2024, Volume and Issue: 15(1), P. 5 - 5

Published: Dec. 24, 2024

This study aimed to investigate the effects of different storage times mixed inoculum on in vitro rumen fermentation characteristics, microbial diversity, and community composition. The experiment was divided into five groups, with composed fresh fluid culture medium being stored at 39 °C for 0 h (H0), 12 (H12), 24 (H24), 36 (H36), 48 (H48). After fermentation, collected assess characteristics H24 group showed higher total gas production, ammoniacal nitrogen levels, volatile fatty acids, as well concentrations individual acids except propionate, compared H0 H48 groups (p < 0.05). Shannon Simpson evenness indices were significantly H0, H12, than A nine phyla sixteen genera involved starch fiber degradation found be more abundant or Moreover, predicted metabolic pathways observed enriched either Both principal coordinates analysis (PCoA) non-metric multidimensional scaling (NMDS) revealed distinct clustering patterns among H24, H36, similarities (ANOSIM) confirmed these significant differences (R = 1.00, p demonstrates that time influences composition a time-dependent manner. It is recommended use has been within an anaerobic environment tests. offers valuable insights strategies inoculum, thereby improving methodologies variable control techniques.

Language: Английский

Dynamic Changes in Rumen Microbial Diversity and Community Composition Within Rumen Fluid in Response to Various Storage Temperatures and Preservation Times DOI Creative Commons
Chang Liu, Jin Cheng,

Y.F. Xie

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(3), P. 234 - 234

Published: March 3, 2025

The aim of this study was to investigate the effects storage temperature and preservation time on microbial diversity community composition rumen fluid. Rumen fluid samples were collected from six Hu sheep fed a high-forage diet stored at −80 °C −20 for intervals 0, 7, 14, 30, 60, 120, 240 days. DNA extracted each point 16S rRNA gene sequencing evaluate composition. results showed that affected only relative abundance Proteobacteria, with no substantial impact alpha-diversity or other groups (p > 0.05), significant interaction observed between 0.05). Alpha-diversity indices such as Chao1, species, PD whole tree dynamic changes after 7 days storage, while abundances Verrucomicrobiota Christensenellaceae R-7 group, well energy metabolism metabolic pathway, exhibited alterations 14 < Notably, Patescibacteria, Rikenellaceae RC9 gut Veillonellaceae UCG-001 demonstrated Both principal coordinates analysis (PCoA) non-metric multidimensional scaling (NMDS) distinct overlaps. This suggests storing does not influence composition, whereas significantly impacts these factors, most differences emerging preservation. Consequently, it is advised in be conducted within post-collection.

Language: Английский

Citations

0
The rumen microbiome and its metabolome together with the host metabolome regulate the growth performance of crossbred cattle DOI Creative Commons
Wei Zhao,

Li-na Ma,

Xue Lin

et al.

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 21, 2025

Although it has been demonstrated that gastrointestinal microorganisms greatly influence livestock performance, the effect of on growth performance crossbred cattle remains unclear. Due to their superior production characteristics, understanding impact beef is significant importance for improving farming efficiency. In this study, healthy Simmental with similar birth date and weight were selected as dams, (Combination I), Belgian Blue II) Red Angus III) used parents crossbreeding. The progeny three combination crosses measured under identical conditions from rearing 18 months age (n = 30). Rumen fluid plasma collected macro-genomic non-targeted metabolomic analysis 8). results showed Combination II was I III in body (BW) height (BH) (P < 0.05). Mycoplasma, Succinivibrio, Anaerostipes, Methanosphaera, Aspergillus, Acidomyces significantly increased rumen 0.05), whereas differentially expressed metabolites (DEMs) 9,10,13-Trihome (11), 9,12,13-Trihome 9(10)-Epome, 9(S)-Hpode reduced abundance. addition, DEM PC (14:0/P-18:1(11Z)), (16:0/0:0), (17:0/0:0) down-regulated II. Correlation revealed Succinivibrio associated 9(S)-Hpode, 9,10,13-Trihome, (17:0/0:0), (16:0/0:0). Growth traits positively correlated dominant genera, while negatively key Our study reveals role its host metabolism regulation cattle, which will contribute development modern breeding.

Language: Английский

Citations

0
Effects of Grape Pomace Complete Pellet Feed on Growth Performance, Fatty Acid Composition, and Rumen Fungal Composition in Beef Cattle DOI Creative Commons

Meiling Teng,

Yuanqiu Li,

Jiangjiao Qi

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(7), P. 930 - 930

Published: March 24, 2025

Grape pomace, a winemaking byproduct, is nutrient- and polyphenol-rich, but research on its use in beef cattle limited. This study explored the impact of grape pomace-based complete pellet feed growth, serum biochemistry, fatty acid profile, rumen microbiota cattle. Fifteen healthy Simmental were randomly divided into three groups (G0, G15, G20) fed pelleted ration containing 0%, 15%, 20% respectively, for 60 days. The results showed that addition pomace to markedly increased average daily intake weight gain In terms levels total protein (TP) albumin (ALB) G20 group higher than G0 (p > 0.05). oleic acid, linoleic behenic group. had no significant effect fungal diversity volatile acids (TVFAs) pH ammonia nitrogen content G15 significantly indicates can be used as raw material

Language: Английский

Citations

0
‘Geophagy’ and Clay Minerals: Influencing Ruminal Microbial Fermentation for Methane Mitigation DOI Creative Commons
Zubaer Hosen, Md. Rashidul Islam, Ravi Naidu

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 866 - 866

Published: April 10, 2025

Methane is a greenhouse gas with high warming potential, and ruminants like cattle sheep are major source of its emission. In the rumen, first stomach compartment, diverse microorganisms fauna live, including archaea, bacteria, protozoa, nematodes, fungi. They participate in complex fermentation processes. During rumen fermentation, various gases produced, dominantly hydrogen carbon dioxide. methanogenesis, methanogens utilize these two to produce methane as byproduct, which burps out into atmosphere. Therefore, interfering this methanogenesis promising way reducing methane. Supplementing feed containing clay minerals could be one method do so naturally consume them they graze, often called “geophagy”. This review discusses role enteric abatement, emphasizing clay–microbial interaction rumen. interactions, also serve carrier for other chemicals influence microbial attachment. Elemental dissolution cations from mineral their buffering capacity can further dynamics fluids. By combining insights microbiology, soil science, animal nutrition, provides an interdisciplinary view interactions. Findings help develop low-cost safe supplement reduce livestock emissions.

Language: Английский

Citations

0
Rumen Microbiota Transplantation Alleviates Gossypol Diet-Induced Reproductive, Liver, and Intestinal Damage in Male Mice DOI Creative Commons
Chen Zhang,

Wenguang Lu,

Huiru Liu

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(15), P. 2206 - 2206

Published: July 30, 2024

Ruminants exhibit stronger tolerance to gossypol, an anti-nutritional factor, compared monogastric animals. We transplanted Hu sheep rumen microbiota into male mice investigate the role of in animal gossypol tolerance. Thirty specific-pathogen-free (SPF) C57BL/6 were randomly divided three groups: normal diet (CK group), (FG and transplantation (FMT group, diet). The pathological changes liver small intestine mice, organ coefficient, sperm parameters analyzed. Alanine aminotransferase (ALT) aspartate (AST) levels blood lactate dihydrogen-X (LDH-X) testicular tissue also measured. results showed that body weight, feed intake, concentration, motility, LDH-X FMT group increased (

Language: Английский

Citations

2
The Effects of Mixed Inoculum Storage Time on In Vitro Rumen Fermentation Characteristics, Microbial Diversity, and Community Composition DOI Creative Commons
Chang Liu,

Jing Ge,

Jiaqi Dai

et al.

Animals, Journal Year: 2024, Volume and Issue: 15(1), P. 5 - 5

Published: Dec. 24, 2024

This study aimed to investigate the effects of different storage times mixed inoculum on in vitro rumen fermentation characteristics, microbial diversity, and community composition. The experiment was divided into five groups, with composed fresh fluid culture medium being stored at 39 °C for 0 h (H0), 12 (H12), 24 (H24), 36 (H36), 48 (H48). After fermentation, collected assess characteristics H24 group showed higher total gas production, ammoniacal nitrogen levels, volatile fatty acids, as well concentrations individual acids except propionate, compared H0 H48 groups (p < 0.05). Shannon Simpson evenness indices were significantly H0, H12, than A nine phyla sixteen genera involved starch fiber degradation found be more abundant or Moreover, predicted metabolic pathways observed enriched either Both principal coordinates analysis (PCoA) non-metric multidimensional scaling (NMDS) revealed distinct clustering patterns among H24, H36, similarities (ANOSIM) confirmed these significant differences (R = 1.00, p demonstrates that time influences composition a time-dependent manner. It is recommended use has been within an anaerobic environment tests. offers valuable insights strategies inoculum, thereby improving methodologies variable control techniques.

Language: Английский

Citations

1