Epidemiology and Molecular Characterisation of Multidrug-Resistant Escherichia coli Isolated from Cow Milk DOI Creative Commons

Zarin Tasnim Mim,

Chandan Nath, Abdullah Al Sattar

et al.

Veterinary Sciences, Journal Year: 2024, Volume and Issue: 11(12), P. 609 - 609

Published: Nov. 29, 2024

Antimicrobial resistance (AMR) is a growing global concern and poses significant threat to public health. The emergence of multidrug-resistant organisms, including Escherichia coli, also presents risk transmission humans through the food chain, milk. This study aimed investigate prevalence E. coli in raw milk Chattogram metropolitan area (CMA) Bangladesh their phenotypic genotypic antimicrobial patterns. A total 450 cow samples were collected from 18 farms within CMA. isolation identification performed following standard bacteriological methods. susceptibility testing (AST) was conducted using Kirby–Bauer disc diffusion method. Molecular detection genes Polymerase Chain Reaction (PCR). found 134 (29.77%) that tested positive for coli. revealed highest rates (69.40%) be ampicillin, amoxicillin–clavulanic acid, cephalothin, cephalexin, with lowest (21.64%) being norfloxacin. correlation (r = 1) observed between ciprofloxacin ceftazidime among antimicrobials tested. All isolates classified as (MDR), resistant three or more classes, multiple index >0.2. PCR amplification showed blaTEM gene had (74.19%) ESBL In contrast, blaCMY-1 lower (6.45%) genes, while tetD (2.9%) Positive correlations presence these genes. emphasises high MDR its potential impact on It underscores urgent need strategic interventions effectively manage mitigate AMR Bangladeshi dairy sector, focusing prudent use implementing enhanced surveillance.

Language: Английский

Molecular detection of Shiga toxin and extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates from sheep and goats DOI Creative Commons
Tsepo Ramatla,

Mpho Tutubala,

Tshepang Motlhaping

et al.

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: Jan. 2, 2024

Abstract Background The Shiga toxin (Stx)-producing Escherichia coli (STEC) have become important global public health concerns. This study investigated the prevalence, antimicrobial resistance profile, and extended-spectrum beta-lactamase-producing E. in sheep goat faeces. Methods results A total of 53 isolates were confirmed by PCR targeting uidA [ β -D glucuronidase] gene. genes stx 1 2, as well bfp A, vir , eae lt aaf II virulence genes, detected this study. Of to be STEC, 100% positive for stx2 47.2% stx1 . Three possessed a combination + 2 while four harboured genes. displayed phenotypic against erythromycin (66.04%), colistin sulphate (43.4%), chloramphenicol (9.4%) ciprofloxacin (1.9%). 28.8% strains phenotypically considered ESBL producers contained beta-lactamase bla CTX-M-9 CTX-M-25 gene groups. larger proportion (86.8%) antibiotic sulphonamide resistant ( sul II) gene, 62.3%, 52.8%, 43.4%, 41.5%, 20.8%, 18.9%, 11.3%, 9.4%, 9.4% 5.7% mcr-4, floR, mcr-1, tet (A), sulI, (O), (W), parC, mcr-2, ampC 5, qnrS ermB respectively. Thirteen ESBL-producing multi-drug (MDR). One ) two groups) present 16 isolates. In conclusion, from small stock large array high profiles. Conclusions Our findings highlight importance goats sources MDR From veterinary medicine perspective, characterization originating livestock (sheep goats) is crucial due their close contact with humans.

Language: Английский

Citations

8
Genetic Characteristics of Antimicrobial Resistance in Escherichia coli Isolated from Farm Animals, Slaughterhouses, and Associated Environments DOI Creative Commons
Debby Fadhilah Pazra,

Dinda Iryawati

IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Antimicrobial resistance (AMR) in Escherichia coli from farm animals, slaughterhouses, and associated environments poses a critical threat to public health food safety. Several studies have reported that antibiotic genes (ARGs) are found with high prevalence, especially blaCTX-M, mcr-1, tetA tetB, aac(6′)-Ib, qnr. These were frequently mobile genetic elements (MGEs) such as IncF IncI plasmids, class 1 integrons, insertion sequences (e.g., IS26), transposons, which facilitate horizontal gene transfer adaptation selective pressures. Comparative analysis indicated animals slaughterhouse act reservoirs convergence points for resistant E. strains diverse sources, amplifying the spread of genes. Environmental samples, including wastewater demonstrated overlap isolates suggesting inadequate waste management contributes AMR propagation has potential zoonotic transmission. This chapter will explain characteristics antibiotic-resistant focus on dominant ARGs MGEs drive dissemination resistance. information is needed monitoring evolution so appropriate policies interventions can be implemented prevent control coli.

Language: Английский

Citations

0
Fecal carriage of ESBL-, carbapenemase- and AmpC- producing Escherichia coli in cattle and sheep in Algeria: Emergence of NDM and OXA-181 DOI

Hassina KIRAT,

Hamza Rahab, Zohra Chekroud

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Abstract Introduction: The spread of third-generation cephalosporin (3GC)-resistant Escherichia coli in food-producing animals poses a significant threat to public health, with limited data from cattle and sheep Algeria. This study investigated the prevalence 3GC-resistant E. coli in Guelma, northeast Methodology: 285 fecal samples were collected (n=145) (n=140) on 28 farms. Samples screened for coli. Antibiotic susceptibility was tested, ESBL carbapenemase production evaluated using double disc EDTA tests. PCR identified resistance integron genes. Results: Twenty-seven cefotaxime-resistant isolates detected 17% bovine 1% ovine samples, spanning 43% Multidrug observed 85% isolates, high β-lactams, tetracyclines, fluoroquinolones, trimethoprim-sulfamethoxazole. following beta-lactamase genes detected: blaCTX-M (74%), blaCMY (44%), blaNDM-1 (37%), blaOXA-181 (4%) identified. Class 1 integrons also ten isolates. Conclusions: These findings emphasize presence ESBL-, AmpC-, carbapenemase-producing among Algerian livestock, highlighting need comprehensive monitoring control manage these resistant bacteria.

Language: Английский

Citations

0
Multidrug-resistance and extended-spectrum beta-lactamase-producing lactose-fermenting enterobacteriaceae in the human-dairy interface in northwest Ethiopia DOI Creative Commons
Achenef Melaku Beyene,

Mucheye Gizachew,

Ahmed E. Yousef

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(5), P. e0303872 - e0303872

Published: May 21, 2024

Background Antimicrobial resistance (AMR) is among the top public health concerns in globe. Estimating prevalence of multidrug (MDR), MDR index (MDR-I) and extended-spectrum beta-lactamase (ESBL)-producing lactose fermenting Enterobacteriaceae (LFE) important designing strategies to combat AMR. Thus, this study was designed determine status MDR, MDR-I ESBL-producing LFE isolated from human-dairy interface northwestern part Ethiopia, where such information lacking. Methodology A cross-sectional conducted June 2022 August 2023 by analyzing 362 samples consisting raw pooled milk (58), container swabs milker’s hand farm sewage (57), stool (47), cow’s feces (84). The were analyzed using standard bacteriological methods. antimicrobial susceptibility patterns ESBL production ability isolates screened Kirby-Bauer disk diffusion method, candidate passing screening criteria phenotypically confirmed cefotaxime (30 μg) /clavulanic acid μg/10 combined-disk test. further characterized genotypically multiplex polymerase chain reaction targeting three ESBL-encoding- genes namely bla TEM , SHV CTX-M . Results total 375 bacterial identified proportion 70.7 21.3%, respectively. varied 0.0 0.81 with an average 0.30. detected all sample types. Genotypically, majority (97.5%), which positive on phenotypic test, carrying one or more genes. Conclusion high MDR; had for production. findings provide evidence that reservoirs AMR traits. Therefore, implementation mitigation highly needed area.

Language: Английский

Citations

3
The Global Rise of ESBL-Producing Escherichia coli in the Livestock Sector: A Five-Year Overview DOI Creative Commons

Antonio Mandujano-Hernández,

Ana Verónica Martínez-Vázquez, Alma D. Paz-González

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(17), P. 2490 - 2490

Published: Aug. 27, 2024

β-lactam antibiotics are a key element in the treatment of bacterial infections. However, excessive use these has contributed to emergence β-lactam-resistant enterobacteria, including

Language: Английский

Citations

3
Prevalence and Molecular Characterization of ESBL/pAmpC Producing Faecal Escherichia coli Strains with Widespread Detection of CTX-M-15 Isolated from Healthy Poultry Flocks in Eastern Algeria DOI

Hafsa Akkari,

Nouzha Heleili, Osman Birol Özgümüş

et al.

Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: unknown, P. 106973 - 106973

Published: Sept. 1, 2024

Language: Английский

Citations

1
Prevalence and molecular characterization of ESBL-producing Escherichia coli isolated from broiler chicken and their respective farms environment in Malaysia DOI Creative Commons

Mulu Lemlem,

Erkihun Aklilu, Maizan Mohamed

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Nov. 26, 2024

Extended spectrum beta-lactamase-producing Escherichia coli (ESBL-EC) is an increasing public health threat. This study aimed to determine the prevalence and characterization of ESBL-producing (E. coli) isolated from broiler chicken their farm environment, in Kelantan Malaysia. was 453 collected samples, including 210 cloacal swabs 243 environmental samples. The antimicrobial susceptibility profile E. isolates assessed for sixteen antibiotics using disc diffusion method. were evaluated phenotypic ESBL production modified double synergy. After extraction genomic DNA, resistance genes, phylogenetic group, virulence genes detected by PCR appropriate primers. further confirmed sequencing. molecular typing strains determined Multilocus Sequence Typing (MLST). A total 93.8% (425/453) Out 334 screened, 14.7% (49/334) phenotypically producers. All ESBL-EC resistant tetracycline, ciprofloxacin, ampicillin. Thus, 100% multidrug resistant. Of 81.6% positive at least one encoding gene. most prevalent gene blaTEM (77.6%; 38/49) followed blaCTX−M (32.7%; 16/49) blaSHV (18.4%; 9/49). majority belonged phylogenic groups B1 accounting 44.9% 12.2%, respectively. frequently identified sequence types ST10 (n = 3) ST206 3). astA (33.3%; 22/66) iss (15.2%; 10/66). Our results show both respective farms environment reservoirs multi-drug genes.

Language: Английский

Citations

1
Molecular characterization of multidrug-resistant (MDR) Escherichia coli in the Greater Accra Region, Ghana: a ‘One Health’ approach. DOI Creative Commons
Michael Olu-Taiwo, Beverly Egyir,

Christian Owusu-Nyantakyi

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 25, 2024

Abstract Background: ‘One health’, a concept that highlights the need to bring on board multiple players and actors together addressing major health problems, have been proposed be effective in data gathering mitigate menace of antimicrobial drug resistance (AMR). Data MDR extended-spectrum-beta-lactamase-producingEscherichia coli(ESBL-EC) across humans, animals environment are limited low-and-middle-income-countries (LMICs) including Ghana.Objective: This study used one approach determine prevalence, antibiogram AMR genes ESBL-ECfrom diverse sources.Methodology: A cross-sectional conducted Accra Tema metropolis, Ghana. We randomly collected 1500 non-duplicated specimens from healthy human, cattle, pigs, lettuce, spring onions, pork, beef soil samples.Escherichia coli(E. coli)was isolated confirmed by MALDI-TOF MS.E. coliisolates were screened for their susceptibility against 13 antibiotic agents ESBL-production. ESBL-ECisolates whole-genome sequenced (WGS) andin silicoanalysis was genes, sequence types (STs) plasmid replicon types.Result: Overall,E. coliwas recovered 140 (9.3%) processed. About one-third theseE. 50 (35.7%) resistant three or more antibiotics, 30 (21.5%) ESBL-EC. The proportion ESBL-ECidentified pig, 14 (20.0%), 9 (22.5%), 3 (15.0%), 1 (50.0%) (37.5%), respectively. NoE. onions pork. Overall, ESBL-ECexhibited high levels ampicillin (100%), cefuroxime ciprofloxacin (53.6%), tetracycline (58.2%). However, all sensitive meropenem. prevalent detected wereblaTEM-1B (32.0%; n=8),tetA (48.0%; n=12) andsul2(32.0%; n=8). dominant STs ST10 (12%; n=3), ST 9312 206 n=3) 4151 n=3). IncFIB(Apoo1918) (40.0%; n=10) IncFII(pCoo) (36.0%; n=9) replicons commonly detected.Conclusion: Within metropolis surveyed, we identified ESBL-ECharbouring various with diverseE. coliSTs environment. finding ofblaCTX-M-15 agricultural isolate is worrisome, emphasizing one-health combating AMR.

Language: Английский

Citations

0
Phenotypic and Molecular Characterization of Escherichia coli Isolated from Food Animals for Antimicrobial Resistance DOI Open Access
Manish Ranjan,

Rahul Kolhe,

C. D. Bhong

et al.

Asian Journal of Dairy and Food Research, Journal Year: 2024, Volume and Issue: Of

Published: Aug. 6, 2024

Background: The use of antimicrobials in food animals significantly contributes to the development antimicrobial resistance nature. This study aimed determine prevalence ESBL-producing E. coli isolated from cattle, buffalo, sheep and goat fecal samples. Methods: In this out 2025 healthy 10 different farms, samples were randomly collected by rectal swabs 200 (50 each goat). was further studied for AMR ESBL production phenotypic assays PCR targeting genes. Result: overall 81.50% with highest isolation rate cattle Out 163 isolates, 45.39% ESBL-producers. Genes encoding beta-lactam viz. blaSHV, blaTEM, blaCTX-M blaOXA detected 44.59% ESBL-positive isolates. blaSHV present only while all animals. genes found as (2.70%), blaTEM (12.16%), (22.97%) (6.75%). A high degree multiple observed. Maximum susceptibility observed trimethoprim, imipenem chloramphenicol. demonstrated that could be source multidrug-resistant coli. findings are significant context monitoring India.

Language: Английский

Citations

0
Epidemiology and Molecular Characterisation of Multidrug-Resistant Escherichia coli Isolated from Cow Milk DOI Creative Commons

Zarin Tasnim Mim,

Chandan Nath, Abdullah Al Sattar

et al.

Veterinary Sciences, Journal Year: 2024, Volume and Issue: 11(12), P. 609 - 609

Published: Nov. 29, 2024

Antimicrobial resistance (AMR) is a growing global concern and poses significant threat to public health. The emergence of multidrug-resistant organisms, including Escherichia coli, also presents risk transmission humans through the food chain, milk. This study aimed investigate prevalence E. coli in raw milk Chattogram metropolitan area (CMA) Bangladesh their phenotypic genotypic antimicrobial patterns. A total 450 cow samples were collected from 18 farms within CMA. isolation identification performed following standard bacteriological methods. susceptibility testing (AST) was conducted using Kirby–Bauer disc diffusion method. Molecular detection genes Polymerase Chain Reaction (PCR). found 134 (29.77%) that tested positive for coli. revealed highest rates (69.40%) be ampicillin, amoxicillin–clavulanic acid, cephalothin, cephalexin, with lowest (21.64%) being norfloxacin. correlation (r = 1) observed between ciprofloxacin ceftazidime among antimicrobials tested. All isolates classified as (MDR), resistant three or more classes, multiple index >0.2. PCR amplification showed blaTEM gene had (74.19%) ESBL In contrast, blaCMY-1 lower (6.45%) genes, while tetD (2.9%) Positive correlations presence these genes. emphasises high MDR its potential impact on It underscores urgent need strategic interventions effectively manage mitigate AMR Bangladeshi dairy sector, focusing prudent use implementing enhanced surveillance.

Language: Английский

Citations

0