Synergistic effects of Pandanus fascicularis extracts and azithromycin: in vitro and in silico antimicrobial investigation against MDR clinical strains DOI Creative Commons
Hajera Khatun, Md Rashedul Islam,

Shikha Khatun

et al.

Frontiers in Antibiotics, Journal Year: 2025, Volume and Issue: 4

Published: May 29, 2025

Background Antibiotic-resistant bacteria are becoming a significant global concern. To combat the spread of resistance or reverse multidrug resistance, developing novel antimicrobials and/or modulators is essential. This study aimed to evaluate synergistic effects methanolic extract Pandanus fascicularis fruits (MEPFF) in combination with azithromycin against multidrug-resistant bacteria. Methods Phytochemical analysis along determination total phenolic content (TPC), flavonoid (TFC), and antioxidant capacity (TAC) MEPFF, was performed using standard procedure. The extract's DPPH free radical scavenging activity assessed its potential activity. minimal inhibitory concentration (MIC) bactericidal (MBC) MEPFF Staphylococcus aureus , Bacillus cereus Escherichia coli Pseudomonas aeruginosa were determined, followed by an effect azithromycin, as well alone. Subsequently, drug-likeness, antibacterial activity, toxicological properties analyzed silico tools. Results quantitative investigation found terpenoids, flavonoids, tannins, phenolics, saponins, cardiac glycosides, alkaloids MEPFF. TFC, TPC, TAC at 183 ± 9.54 mg QE, 248.33 11.06 GAE, 95.33 8.33 AAE/gm extract. showed experiment, IC50 value 12.13±0.53 µg/ml. Azithromycin together have far greater action all four bacterial strains. 3.67 1.15 5.83 0.76 mg/mL, while minimum 4.33 1.26 7.33 1.04 mg/mL. In studies revealed that pandamarilactone-1, nonpandamarilactone-B, thiamine had best docking energy (−9.9, −8.9, −8.5 kcal/mol), suggesting most active compounds MPh-II protein. Conclusion enhances antibiotic therapy suggests aforesaid drug-herb combinations may treat MDR infections.

Language: Английский

Evaluation of Larrea tridentata Extracts and Their Antimicrobial Effects on Strains of Clinical Interest DOI Open Access

Renata Morales-Márquez,

Lucía Delgadillo-Ruíz, Alfredo Esparza-Orozco

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1032 - 1032

Published: Jan. 25, 2025

The use of medicinal plants represents an alternative method for bacterial control due to their chemical compositions. This study’s objective was determine the inhibitory capacity Larrea tridentata extracts against microbial strains clinical interest. Four were prepared, phytochemical profiles determined, and antioxidant capacities quantified. Additionally, minimum concentrations hemolysis determined using human blood erythrocytes. For extracts’ growth capacity, six two fungal evaluated disk diffusion test. Commercial medications specific each strain used as controls. ethanolic registered greatest diversity metabolites related antibacterial activity. activities extract Cedax® similar Enterococcus faecalis. A principal component analysis performed with X2 ANOVA tests identify relationships effects on inhibition, obtaining p > 0.05 a confidence level 95%. research highlights potential L. treatment mitigate growing problem resistance traditional antibiotics.

Language: Английский

Citations

2
Current strategies against multidrug-resistant Staphylococcus aureus and advances toward future therapy DOI Creative Commons
Saurabh Kumar, Richa Prasad Mahato,

Sanjay Ch

et al.

The Microbe, Journal Year: 2025, Volume and Issue: unknown, P. 100281 - 100281

Published: Feb. 1, 2025

Language: Английский

Citations

2
Fungal Biomolecules for Food and Pharmaceutical Application DOI Creative Commons
Giancarlo Angeles Flores, Gaia Cusumano, Gökhan Zengin

et al.

eFood, Journal Year: 2025, Volume and Issue: 6(1)

Published: Jan. 20, 2025

ABSTRACT Mushrooms have long been an integral part of human culture and health are valued for their nutritional therapeutic properties. Often referred to as a superfood, mushrooms rich in bioactive compounds that provide range benefits including antioxidant, antimicrobial, anticancer, prebiotic, antidiabetic, anti‐inflammatory effects. This review focussed on the diversity mushrooms, emphasizing medicinal significance, explores secondary metabolites with potential applications nutraceuticals pharmaceuticals. It underscores role vital agents developing value‐added products. An overview compounds, such polysaccharides, peptides, proteins, terpenoids, phenolic identified numerous mushroom species. Additionally, addresses advancements fungal biotechnology, particularly use fungi efficient industrial cell factories, influence food quality sustainability. By critically analyzing recent studies, summarizes diverse roles biomolecules pharmaceutical industries highlights significant contribution modern medicine health‐related fields.

Language: Английский

Citations

1
Frontiers in superbug management: innovating approaches to combat antimicrobial resistance DOI
Priyanka Chambial, Neelam Thakur, Prudhvi Lal Bhukya

et al.

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(3)

Published: Feb. 14, 2025

Language: Английский

Citations

1
LC-MS analysis and antioxidant, antibacterial, and antidiabetic activity of Jumli Marshi rice from Nepal: An in vitro and in silico investigation to validate their potential as a functional food DOI Creative Commons
Ram Kishor Yadav,

Rekha Bhandari,

Hellión-Ibarrola M.C.

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(3), P. e0319338 - e0319338

Published: March 10, 2025

Jumli Marshi (J. Marshi) , a native rice cultivar in Nepal, is gaining popularity owing to its health benefits for obesity, hypertension, and diabetes. However, scientific evidence verifying therapeutic potential lacking until November 2024. Therefore, we aimed characterize the phytoconstituents evaluate antioxidant, antibacterial, antidiabetic properties of J. Marshi, along with ADME toxicity profile, using both vitro silico approaches. Liquid chromatography-mass spectrometry analysis 70% methanol extract from identified ten plant-based compounds, including phenolic acids, flavonoids, γ-oryzanol. The exhibited significant antioxidant properties, neutralizing DPPH free radicals fifty-percentage inhibitory concentration (IC 50 ) 42.65 ± 3.9 µg/mL, compared ascorbic acid’s IC 4.12 0.7 µg/mL. It also showed antibacterial activity against Staphylococcus aureus zone inhibition (ZOI) ranging 7 11 mm minimum (MIC) 1.56 mg/mL, standard antibiotics meropenem (ZOI: 24 1.6 mm; MIC: mg/mL). enzymatic assay demonstrated that inhibits fifty percent enzyme at (EC > 1000 µg/mL α -amylase 250 2.5 -glucosidase, contrast acarbose, exhibiting an EC 35.5 1.5 189.5 1.9 -glucosidase. In docking studies revealed strong interactions target protein catalytic residues, particularly gamma-oryzanol (−10.0 kcal/mol) chlorogenic acid -glucosidase (−7.7 kcal/mol), acarbose (−6.9 −7.1 kcal/mol). suggested tricin had best drug-likeness safety profiles. To our knowledge, this first study reveal presence bioactive acids flavonoids. Furthermore, it offers supporting -glucosidase-inhibitory confirming applications as functional food used management

Language: Английский

Citations

1
Essential oils and plant-derived bioactive compounds: a comprehensive review of their therapeutic potential, mechanisms of action, and advances in extraction technologies DOI
Sameh S. Ali,

Rania Al-Tohamy,

Majid Al-Zahrani

et al.

Phytochemistry Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: May 14, 2025

Language: Английский

Citations

1
Antimicrobial Peptides: The Game-Changer in the Epic Battle Against Multidrug-Resistant Bacteria DOI Creative Commons
‏Helal F. Hetta,

Nizar Sirag,

Shumukh M. Alsharif

et al.

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(11), P. 1555 - 1555

Published: Nov. 20, 2024

The rapid progress of antibiotic resistance among bacteria has prompted serious medical concerns regarding how to manage multidrug-resistant (MDR) bacterial infections. One emerging strategy combat is the use antimicrobial peptides (AMPs), which are amino acid chains that act as broad-spectrum molecules and essential parts innate immune system in mammals, fungi, plants. AMPs have unique antibacterial mechanisms offer benefits over conventional antibiotics combating drug-resistant Currently, scientists conducted multiple studies on for infections found a promising alternative antibiotics. On other hand, can develop several tactics resist bypass effect AMPs. Therefore, it like battle between community AMPs, but who will win? This review provides thorough insights into development well detailed information about terms their history classification. Furthermore, addresses action these mechanisms, ensure win this battle. Finally, updated FDA-approved those were still clinical trials. vital researchers therapeutic application novel

Language: Английский

Citations

6
Synthesis, biological evaluation, molecular docking analyses, and ADMET study of azo derivatives containing 1-naphthol against MβL-producing s. Maltophilia DOI Creative Commons

Zahraa Falah Azeez,

Luay Ali Khaleel, Hussein Ali Kadhim Kyhoiesh

et al.

Results in Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 101864 - 101864

Published: Oct. 1, 2024

Language: Английский

Citations

5
Clinical Trial Findings and Drug Development Challenges for Curcumin in Infectious Disease Prevention and Treatment DOI Creative Commons
Mohamed El Oirdi, Mohd Farhan

Life, Journal Year: 2024, Volume and Issue: 14(9), P. 1138 - 1138

Published: Sept. 9, 2024

Since ancient times, turmeric, scientifically known as Curcuma longa, has been renowned for its therapeutic properties. Recently, extensive documentation highlighted the prevalence of microbial diseases without effective treatments, increased expense certain antimicrobial medications, and growing occurrence drug resistance. Experts predict that resistance will emerge a significant global cause death by middle this century, thereby necessitating intervention. Curcumin, major curcuminoid molecule, shown action. Improving altering use natural agents is most approach to addressing issues targeted specificity in chemically synthesized medicines. Further research required explore efficacy curcumin other substances combating infections. The solubility bioavailability impede capability. To enhance curcumin’s effectiveness, researchers have recently employed several methods, including development curcumin-based nanoformulations. This review seeks compile latest available literature assess advantages agent (particularly antiviral antibacterial) strategies medical efficacy. future application help alleviate infections, promoting sustainability world’s population.

Language: Английский

Citations

4
Almond Hull Extract Valorization: From Waste to Food Recovery to Counteract Staphylococcus aureus and Escherichia coli in Formation and Mature Biofilm DOI Creative Commons
Sara D’Arcangelo, Debora Santonocito, Luciano Messina

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(23), P. 3834 - 3834

Published: Nov. 28, 2024

The increase in food waste accumulation needs innovative valorization strategies that not only reduce environmental impacts but also provide functional applications. This study investigates the potential of almond hulls, an abundant agricultural by-product, as a source bioactive compounds. For first time, hull extract (AHE), was evaluated terms anti-adhesive and anti-biofilm activity against Staphylococcus aureus ATCC 29213 Escherichia coli 9637. obtained by optimized eco-friendly green technique using ultrasound-assisted extraction (UAE), it characterized for its main compounds high-performance liquid chromatography–mass spectrometry (HPLC-MS) nuclear magnetic resonance (NMR) analysis. Antimicrobial on planktonic cells minimum inhibitory/bactericidal concentration (MIC/MBC) 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays. Afterward, AHE bacterial sessile phase, both in-formation mature biofilm. Finally, toxicity tested normal human adult (HDFa) MTT test. principal active present belong to polyphenol group, particular, phenolic acid (Hydroxycinnammic sub-class) and, more significantly, flavonoid class. results showed has relevant antimicrobial strains. Moreover, significantly inhibited adhesion promoted biofilm removal, highlighting sustainable agent. test fibroblasts is toxic cells. research highlights how could have high field.

Language: Английский

Citations

3