Theriogenology, Journal Year: 2023, Volume and Issue: 215, P. 234 - 240
Published: Dec. 9, 2023
Language: Английский
Animals, Journal Year: 2025, Volume and Issue: 15(7), P. 962 - 962
Published: March 27, 2025
Cryopreservation is a widely used technique for long-term semen storage; however, it can induce oxidative stress, compromising sperm quality. This study examines the effect of Kaempferia parviflora (KP) supplementation in extenders on post-thaw quality Thai native bulls. Fresh was collected and evaluated motility, viability, concentration prior to cryopreservation. Semen samples were allocated four treatment groups: 0 mg/mL (control), 0.5 mg/mL, 1 1.5 KP. Subsequently, frozen, then thawed, analyzed membrane integrity, stress. The results showed that with 1.0 KP significantly improved motility (46.29 ± 2.66%) viability (43.42 2.15%) compared control (40.77 2.76% 38.63 2.66%, respectively). Membrane integrity also enhanced (47.64 1.18% vs. 41.85 1.98% control), while stress levels reduced (MDA concentration: 2.33 0.29 µM/mL 2.73 0.33 control). However, highest (1.5 KP) negatively affected quality, (36.97 3.32%), (30.88 3.02%), (35.64 1.61%). These findings suggest optimal improving cryopreservation protocols
Language: Английский
Citations
0
Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12
Published: April 16, 2025
Reproductive health is a critical determinant of livestock productivity and economic sustainability. However, it often compromised by infectious diseases, environmental stressors, nutritional deficits. Phytogenic extracts-bioactive compounds derived from medicinal plants-have emerged as sustainable alternatives to synthetic antibiotics hormones, exhibiting antimicrobial, antioxidant, immunomodulatory properties. These extracts influence key reproductive processes such follicular development, oocyte maturation, endometrial while mitigating the detrimental effects oxidative stress pathogenic infections. Recent findings suggest that phytogenic extract can enhance performance, improve quality, support pregnancy outcomes. Despite growing body evidence, optimal application strategies full breadth their biological remain insufficiently explored. This review focuses on molecular mechanisms modulated extracts, particularly in context hormone regulation, immune modulation, mitigation. We also identify knowledge gaps propose future research directions optimize use approach enhancing health.
Language: Английский
Citations
0
Animal Reproduction, Journal Year: 2025, Volume and Issue: 22(2)
Published: Jan. 1, 2025
It remains a problem to efficiently improve the boar sperm quality of liquid storage due reactive oxygen species (ROS) accumulation. To reduce effects ROS on sperm, in this study, 1 μg/mL zinc oxide nanoparticles (ZnO NPs) was added into extender semen during at 4°C and 17°C for 7 days. The finding revealed that motility, viability, plasma membrane integrity (PMI) acrosome significantly increased when compared with control group (P ˂ 0.05) Additionally, ZnO NPs levels adenosine triphosphate (ATP), mitochondrial potential (MMP), antioxidation abilities sperm. Moreover, could protect from oxidative stress (OS) by inhibiting ROS-induced decrease phosphorylation PKA substrates (P-PKAs). Together, current results suggest be used as novel antioxidant agent preservation, which is helpful improving application assisted reproductive technology domestic animals.
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 4129 - 4129
Published: April 8, 2024
Kaempferol (KAE) is a natural flavonoid with powerful reactive oxygen species (ROS) scavenging properties and beneficial effects on ex vivo sperm functionality. In this paper, we studied the ability of KAE to prevent or ameliorate structural, functional oxidative damage frozen–thawed bovine spermatozoa. The analysis focused conventional quality characteristics prior following thermoresistance tests, namely profile semen alongside capacitation patterns, levels key proteins involved in signaling. Semen samples obtained from 30 stud bulls were frozen presence 12.5, 25 50 μM compared native ejaculates (negative control—CtrlN) as well cryopreserved absence (positive control—CtrlC). A significant post-thermoresistance test maintenance motility (p < 0.001), membrane 0.001) acrosome integrity mitochondrial activity DNA was observed supplementation all doses comparison CtrlC. Experimental groups supplemented presented significantly lower proportion prematurely capacitated spermatozoa when decrease superoxide radical recorded administration 12.5 0.05) 0.01). At same time, cryopreservation medium led stabilization Mg2+-ATPase Na+/K+-ATPase 0.0001) Western blot revealed that prevented loss protein kinase (PKA) C (PKC), which are intricately process activation. conclusion, may speculate particularly efficient protection metabolism during through its promote energy synthesis while quenching excessive ROS protect enzymes hyperactivation. These provide supplementary undergoing freeze–thaw process.
Language: Английский
Citations
3
Open Life Sciences, Journal Year: 2022, Volume and Issue: 17(1), P. 1001 - 1029
Published: Jan. 1, 2022
Abstract Bacterial colonization of male reproductive tissues, cells, and fluids, the subsequent impact bacteria on sperm architecture, activity, fertilizing potential, has recently gained increased attention from medical scientific community. Current evidence strongly emphasizes fact that presence in semen may have dire consequences resulting fertility. Nevertheless, molecular basis underlying bacteriospermia-associated suboptimal quality is sophisticated, multifactorial, still needs further understanding. adhesion agglutination immobilization represent most direct pathway sperm–bacterial interactions. Furthermore, release bacterial toxins leukocytic infiltration, associated with a massive outburst reactive oxygen species, been repeatedly dysfunction bacteria-infested semen. This review serves as summary present knowledge subfertility. we strived to outline currently available methods for assessing profiles promising strategies prevention and/or management bacteriospermia practice.
Language: Английский
Citations
16
Reproduction Fertility and Development, Journal Year: 2022, Volume and Issue: 35(3), P. 265 - 281
Published: Dec. 15, 2022
The cryopreservation of spermatozoa is an important reproductive technology for the preservation fertility in man and animals. Since serendipitous discovery glycerol as effective cryoprotectant 1947, sperm has undergone many changes terms freezing methods employed, rates at which samples are frozen thawed, media used to preserve functionality DNA integrity. An extensive literature survey been conducted addressing cryoprotectants employed both animal human semen protocols utilised. results indicate that remains dominant cryoprotective agent, usually incorporated into a balanced salt solution containing energy substrates, buffers, osmolytes protein form serum albumin (human) or skimmed milk (animal). Realisation some damage observed cryostored cells involves generation reactive oxygen species during thawing process, prompted studies assess relative merits incorporating antioxidants media. However, absence systematic comparisons, there currently no consensus antioxidant combination might be most effective. Utilising our fundamental understanding cryodamage optimise each will future.
Language: Английский
Citations
15
Veterinary Research Communications, Journal Year: 2023, Volume and Issue: 47(3), P. 1629 - 1640
Published: March 28, 2023
Language: Английский
Citations
9
Animals, Journal Year: 2022, Volume and Issue: 12(9), P. 1130 - 1130
Published: April 28, 2022
Cryopreservation and storage of semen for artificial insemination (AI) result in excessive accumulation reactive oxygen species (ROS). This leads to a shortened life span reduced motility spermatozoa post-thawing, with consequent impairment their function. However, certain levels ROS are essential facilitate the capacitation required successful fertilisation. Tannins, as well-known antioxidant compounds, may act binders/acceptors/scavengers inhibit damaging effects ROS. review comprises an analysis cryopreservation protocol health functions tannins, well on fresh cryopreserved semen’s longevity Additionally, we surveyed available evidence tannin extract feed supplementation male fertility. We furthermore interrogated existing theories use potential additive extenders, its relationship quality, what degree have been investigated develop testable new hypotheses. Emphasis was placed tannins ROS, involvement regulating sperm structure function during cryopreservation, post-thaw motility, capacitation, fertilising ability. The diverse reproductive system metal ion chelation, protein precipitation, biological abilities identified. current data first support further investigation incorporation tannin-rich plant extracts into extenders enhance survival, ability spermatozoa.
Language: Английский
Citations
13
Natural Product Research, Journal Year: 2022, Volume and Issue: 37(16), P. 2725 - 2731
Published: Sept. 15, 2022
This study investigated the phenolic compounds and antioxidant capacity of fruit extracts Opuntia dillenii (Ker Gawl.) Haw.(OD) ficus-indica (L.) Mill.(OFI), yellow (F1) red (F2) varieties. In order to evaluate activity these on human sperm quality after thawing, semen parameters (vitality, motility, acrosome reaction, oxidative stress, DNA fragmentation) were analysed 1 h exposure. The results showed that OD has higher content power than OFI, they are in F2 F1. Furthermore, regarding thawed sperm, a significant increase motility samples treated with OFI F1 extracts, while an improvement vitality reaction reduction fragmentation observed all exposed compared control. Finally, stress was
Language: Английский
Citations
13
Reproduction in Domestic Animals, Journal Year: 2023, Volume and Issue: 58(5), P. 637 - 645
Published: Feb. 20, 2023
The aim of this study was to investigate the effect Spirulina platensis (SP) and Salvia verbenaca (SV) extracts added skimmed milk (SM) extender on ram sperm quality fertility. Semen collected using an artificial vagina, extended in SM reach a final concentration 0.8 × 109 spermatozoa/mL, stored at 4°C evaluated 0, 5 H 24 H. experiment has been performed three steps. Firstly, from four (methanol: MeOH, acetone: Ac, ethyl acetate: EtOAc hexane: Hex) SP SV, only acetonic hexanoic methanolic SV showed highest vitro antioxidant activities were then selected for following step. Thereafter, effects concentrations (1.25, 3.75, 6.25, 8.75 μg/mL) each extract motility evaluated. output trial led select best having beneficial parameters (viability, abnormalities, membrane integrity, lipid peroxidation) fertility after insemination. results that same (1.25 both Ac-SP Hex-SP, as well 3.75 μg/mL Ac-SV 6.25 MeOH-SV, maintain all during storage. Besides, no difference found between control. In conclusion, shown improve rate insemination similar or competitive many previous studies published field.
Language: Английский
Citations
7