Transcriptomic Modulation Reveals the Specific Cellular Response in Chinese Sea Bass (Lateolabrax maculatus) Gills under Salinity Change and Alkalinity Stress

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(6), P. 5877 - 5877

Published: March 20, 2023

Salinity and alkalinity are among the important factors affecting distribution, survival, growth physiology of aquatic animals. Chinese sea bass (Lateolabrax maculatus) is an aquaculture fish species in China that can widely adapt to diverse salinities from freshwater (FW) seawater (SW) but moderately highly alkaline water (AW). In this study, juvenile L. maculatus were exposed salinity change (SW FW) stress (FW AW). Coordinated transcriptomic responses gills investigated based on weighted gene co-expression network analysis (WGCNA), 8 11 stress-responsive modules (SRMs) identified for stress, respectively, which revealed a cascade cellular oxidative osmotic gills. Specifically, four upregulated SRMs enriched with induced differentially expressed genes (DEGs) mainly corresponding functions "extracellular matrix" "anatomical structure", indicating strong response water. Both "antioxidative activity" "immune response" downregulated SRMs, comprised inhibited specific DEGs, revealing severely disrupted immune antioxidative under stress. These alkaline-specific not groups only osmoregulation Therefore, results correlated regulation process saline-alkaline water, may have arisen through functional divergence adaptive recruitment will provide vital insights development cultivation

Language: Английский

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Effects of Cold Stress on the Hemolymph of the Pacific White Shrimp Penaeus vannamei

Fishes, Journal Year: 2024, Volume and Issue: 9(1), P. 36 - 36

Published: Jan. 16, 2024

Temperature is an important factor in the physiological processes of aquatic organisms and can seriously affect several chemical biological their bodies, including respiratory metabolism, antioxidant capacity, immune signal transduction. In this study, physiological, transcriptomic, metabolomic analyses were used to investigate response Penaeus vannamei cold stress. The results indicated that stress disrupted nucleotide metabolism inhibited gluconeogenesis. However, shrimp exhibited mechanisms order enhance tolerance, regulating changes key genes metabolites amino acid lipid increase energy supply protect cell membrane stability. addition, included Ca2+ pumps ion channels maintain intracellular homeostasis osmotic balance. When temperature dropped further, oxidative damage occurred due overwhelming defense system, function was inhibited. This research provides some references regarding molecular involved responding potential strategies improve tolerance P. vannamei; these are for studying shrimp.

Language: Английский

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Strain-Specific Benefits of Bacillus on Growth, Intestinal Health, Immune Modulation, and Ammonia-Nitrogen Stress Resilience in Hybrid Grouper

Antioxidants, Journal Year: 2024, Volume and Issue: 13(3), P. 317 - 317

Published: March 6, 2024

In the dynamic field of intensive aquaculture, strategic application probiotics has become increasingly crucial, particularly for enhancing resistance to environmental stressors such as ammonia-nitrogen. Over a 42-day period, this study investigated effects different probiotic strains—Bacillus subtilis (BS, 6-3-1, and HAINUP40)—on health resilience hybrid groupers. Each strain, distinct in its origin, was assessed influence on growth performance, antioxidant capacity, immune gene expressions, ammonia-nitrogen stress response grouper. The experimental design included control group three groups, each supplemented with 1 × 108 CFU/g respective strains, respectively. Our results demonstrated notable differences parameters, including final body weight (FBW) feed conversion ratio (FCR). 6-3-1 originating from grouper, exhibited significant improvements growth, oxidative intestinal health. Conversely, BS strain achieved highest survival rates under stress, indicating superior ability regulate inflammatory responses despite less pronounced growth-promoting effects. HAINUP40 distinguished enhancement health, though it also showed activation genes decreased after extended feeding. uniqueness lies detailed examination strain-specific fish context challenge contemporary aquaculture. research revealed that host-derived probiotics, provided more comprehensive benefits performance resilience. contrast, strains varying efficiencies, excelling promoting gut conclusion, underscores complex roles contributing understanding applications presenting new approaches address challenges farming.

Language: Английский

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Transcriptome Analysis to Study the Molecular Response in the Gill and Hepatopancreas Tissues of Macrobrachium nipponense to Salinity Acclimation

Frontiers in Physiology, Journal Year: 2022, Volume and Issue: 13

Published: May 25, 2022

Macrobrachium nipponense is an economically important prawn species and common in Chinese inland capture fisheries. During aquaculture, M. can survive under freshwater low salinity conditions. The molecular mechanism underlying the response to acclimation remains unclear this species; thus, study, we used Illumina RNA sequencing platform for transcriptome analyses of gill hepatopancreas tissues exposed stress [0.4‰ (S0, control group), 6‰ (S6, 12‰ (S12, high group)]. Differentially expressed genes were identified, several adaptation-related terms signaling pathways found be enriched, such as “ion transport,” “oxidative phosphorylation,” “glycometabolism.” Quantitative real-time PCR demonstrated participation 12 key osmotic pressure regulation acute stress. Further, role carbonic anhydrase was investigated by subjecting situ hybridization. Collectively, results reported herein enhance our understanding mechanisms via which adapts changes salinity.

Language: Английский

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The effects of dietary curcumin on growth, antioxidant, transcriptome, and metabolomics in Macrobrachium rosenbergii fed a high plant-protein diet

Aquaculture Reports, Journal Year: 2025, Volume and Issue: 42, P. 102782 - 102782

Published: April 8, 2025

Language: Английский

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Integrative transcriptomic and metabolomic analyses reveal preliminary molecular mechanisms of gills response to salinity stress in Micropterus salmoides

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742600 - 742600

Published: April 1, 2025

Language: Английский

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Transcriptome analysis reveals the oxidative damage and immune-suppression of leech (Whitmania pigra Whitman) intestine induced by high-temperature stress

Aquaculture Reports, Journal Year: 2024, Volume and Issue: 35, P. 102026 - 102026

Published: March 12, 2024

Temperature is a key component in regulating the survival and growth of aquatic animals. However, mechanism high-temperature stress Whitmania pigra Whitman has received little attention. Therefore, aim this study was to investigate possible mechanisms W. using transcriptome analysis detection antioxidant markers. Samples were collected at 1 d, 3 5 7 d under two experimental groups: control group 27 °C 35 °C. High-temperature caused oxidative damage leech intestine, as indicated by decreased enzyme activities (superoxide dismutase total capacity) increased lipid hydroperoxide malondialdehyde. Transcriptome revealed 425 DEGs intestine during stress, with 275 up-regulated 150 down-regulated genes. According KEGG enrichment analysis, involved immune system induced abnormal expression disease-related Furthermore, when leeches exposed B-cell lymphoma-2, heat shock protein 70, 90, nuclear factor erythroid 2-related 2 mRNAs dramatically upregulated, whereas activating transcription factor, inhibitor Nuclear kappa-B, sequestosome 1, toll-like receptor 4 significantly downregulated. In conclusion, impaired intestinal function decreasing capacity, causing damage, suppressing activation capacity. This contributes future research on detailed leeches.

Language: Английский

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Effects of high fat in the diet on growth, antioxidant, immunity and fat deposition of Macrobrachium rosenbergii post-larvae

Fish & Shellfish Immunology, Journal Year: 2022, Volume and Issue: 129, P. 13 - 21

Published: Aug. 19, 2022

Language: Английский

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The role of the renin-angiotensin system (RAS) in salinity adaptation in Pacific white shrimp (Litopenaeus vannamei)

Frontiers in Endocrinology, Journal Year: 2022, Volume and Issue: 13

Published: Dec. 15, 2022

The renin-angiotensin system (RAS) is a hormonal that plays an important role in the regulation of blood pressure and cardiovascular homeostasis mammals. In fishes, RAS pathway participates osmoregulation salinity adaptation. However, invertebrates, particularly crustaceans, remains unknown. this study, four key genes (LV-ACE, LV-APN, LV-AT1R, LV-RR) were cloned, characterized, their expression levels detected eyestalk, hepatopancreas, muscle Litopenaeus vannamei during long-term short-term low stress. results showed LV-ACE, LV-RR encode 666, 936, 175, 323 amino acids, respectively. Low stress downregulated L. vannamei, indicating was suppressed under salinity. Moreover, these play roles drinking rate, controlling urine output, glucose, pressure, downregulation probably affected shrimps. These findings provide novel insights into mechanism adaptation vannamei.

Language: Английский

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Multi-Effects of Acute Salinity Stress on Osmoregulation, Physiological Metabolism, Antioxidant Capacity, Immunity, and Apoptosis in Macrobrachium rosenbergii

Antioxidants, Journal Year: 2023, Volume and Issue: 12(10), P. 1836 - 1836

Published: Oct. 7, 2023

Salinity stress can trigger a series of physiological changes. However, the mechanism underlying response to acute salinity in Macrobrachium rosenbergii remains poorly understood. In this study, osmoregulation, metabolism, antioxidant capacity, and apoptosis were examined over 96 h stress. Hemolymph osmolality increased with increasing salinity. After 48 exposure, glucose, triglycerides, total protein, cholesterol contents two groups (13 26‰ salinity) significantly lower than those 0‰ group. The highest levels these parameters detected at 6 h; however, superoxide dismutase (SOD), capacity (T-AOC), malondialdehyde (MDA) lowest 13‰ activity immunity-related enzyme alkaline phosphatase (AKP) showed decreasing trend remained low level group throughout experiment. No significant differences observed aspartate aminotransferase (AST), alanine (ALT), or lysozyme (LZM) among three treatments h. treatments, gill filament diameter decreased, more pronounced terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive signal was compared that Expression apoptosis-related genes, including Cysteine-aspartic acid protease 3 (Caspase 3), 8 8), Cytochrome c (Cyt-c), tumor suppressor gene (P53), Nuclear factor kappa-B (NF-κB), B cell lymphoma 2 ovarian killer (Bok) higher other 24 h, but Cyt-c P53 exhibited positive relationship MDA, AST, LZM during group, Bok expression correlated SOD, T-AOC, AKP, phosphatase, activity, whereas AST content positively Caspase 8, Cyt-c, expression. A negative between catalase (CAT) activity. These findings provide insight into mechanisms will contribute improving M. aquaculture management practices.

Language: Английский

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