The Impact of NO2 on Epithelial Barrier Integrity of a Primary Cell‐Based Air–Liquid Interface Model of the Nasal Respiratory Epithelium DOI Creative Commons
Helena Moratin,

Josephine Lang,

Mellissa Picker

et al.

Journal of Applied Toxicology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

ABSTRACT Nitrogen dioxide (NO 2 ) is a pervasive gaseous air pollutant with well‐documented hazardous effects on health, necessitating precise toxicological characterization. While prior research has primarily focused lower airway structures, the upper airways, serving as first line of defense against airborne substances, remain understudied. This study aimed to investigate functional NO exposure alone or in combination hypoxia secondary stimulus nasal epithelium and elucidate its molecular mechanisms because considered pathophysiological factor onset persistence chronic rhinosinusitis, disease airways. Air–liquid interface cell cultures derived from primary mucosa cells were utilized an vitro model, offering high vitro–in vivo correlation. Our findings demonstrate that induces malfunction epithelial barrier, evidenced by decreased transepithelial electrical resistance increased fluorescein isothiocyanate (FITC)‐dextran permeability. mRNA expression analysis revealed significant increase IL‐6 IL‐8 expressions following . Reduced tight junction component occludin was identified structural correlate damaged barrier. Notably, hypoxic conditions did not alter barrier integrity. These provide information harmful human epithelium, including compromised integrity induction inflammatory responses. Overall, this contributes our understanding underlying also respiratory diseases associated pollution emphasizes importance mitigating emissions safeguard health.

Language: Английский

Neutrophil-Derived Proteases in Lung Inflammation: Old Players and New Prospects DOI Open Access

Coby J. Cheetham,

Michael C. McKelvey, Daniel F. McAuley

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(10), P. 5492 - 5492

Published: May 17, 2024

Neutrophil-derived proteases are critical to the pathology of many inflammatory lung diseases, both chronic and acute. These abundant enzymes play roles in key neutrophil functions, such as extracellular trap formation reactive oxygen species release. They may also be released, inducing tissue damage loss function. Historically, serine (NSPs) have been main subject protease research. Despite highly promising cell-based animal model work, clinical trials involving inhibition NSPs shown mixed results disease patients. As such, cutting edge neutrophil-derived research has shifted that had little-to-no neutrophils date. include cysteine cathepsins, metzincins calpains, among others. This review aims outline previous work carried out on NSPs, including shortcomings some inhibitor-orientated trials. Our growing understanding other involved function neutrophilic inflammation will then discussed. Additionally, potential targeting these more obscure highlighted, they represent new targets for inhibitor-based treatments neutrophil-mediated inflammation.

Language: Английский

Citations

14
Sentinel supervised lung-on-a-chip: A new environmental toxicology platform for nanoplastic-induced lung injury DOI
Sheng Yang, Tianyi Zhang, Yiling Ge

et al.

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 458, P. 131962 - 131962

Published: June 29, 2023

Language: Английский

Citations

18
Mechanism of circRNA_SMG6 mediating lung macrophage ECM degradation via miR-570-3p in microplastics-induced emphysema DOI Creative Commons

Xiaoxue Sun,

Tian Xiao, Junjie Qin

et al.

Environment International, Journal Year: 2024, Volume and Issue: 187, P. 108701 - 108701

Published: April 25, 2024

Microplastics (MPs) are plastic particles < 5 mm in diameter, of which polystyrene microplastics (PS-MPs) representative type. The extracellular matrix (ECM) degradation macrophages is associated with the development emphysema. Additionally, circular RNAs (circRNAs) have a regulatory role epigenetic mechanisms related to lung disease. However, ECM and circRNAs MPs-induced emphysema still unclear. In our study, Sprague-Dawley (SD) rats were treated 0, 0.5, 1.0 2.0 mg/m

Language: Английский

Citations

5
Cathepsin S: A key drug target and signalling hub in immune system diseases DOI

D. Evangelin Geetha,

Tom Skaria

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 155, P. 114622 - 114622

Published: April 14, 2025

Language: Английский

Citations

0
A549 cells exposed to a marijuana smoke extract affect mono-layer integrity related to oxidative stress state DOI

Elvira Gómez-Guerrero,

Yazmín Debray-García,

Octavio Gamaliel Aztatzi-Aguilar

et al.

Toxicology in Vitro, Journal Year: 2025, Volume and Issue: unknown, P. 106072 - 106072

Published: April 1, 2025

Language: Английский

Citations

0
Urolithin-A supplementation alleviates sepsis-induced acute lung injury by reducing mitochondrial dysfunction and modulating macrophage polarization DOI

Mohd Mohsin,

Almaz Zaki,

Gulnaz Tabassum

et al.

Mitochondrion, Journal Year: 2025, Volume and Issue: unknown, P. 102047 - 102047

Published: May 1, 2025

Language: Английский

Citations

0
Efficacy and safety of Cordyceps sinensis (Hirsutella sinensis, Cs-C-Q80) in chronic bronchitis DOI Creative Commons

Xinyang Shu,

Dongfeng Xu,

Yu-Min Qu

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 13, 2024

Background: Cordyceps sinensis is a traditional Chinese medicine that has shown promise for the management of chronic bronchitis (CB). We aim to assess efficacy and safety preparation C named Bailing capsule ( Hirsutella , Cs-C-Q80) compared with placebo in patients CB. Methods: This randomized, double-blind, placebo-controlled, parallel-group clinical trial (Chinese Clinical Trial Registry; registration number: ChiCTR1900025707) recruited CB from eight hospitals China between May 2019 December 2020. Patients were randomized 2:1 receive or orally 48 weeks (2.0 g, three times day). Results: Among 240 who 238 (Bailing capsule: 159, placebo: 79) included primary analysis. significantly reduced frequency acute exacerbation (AECB) during treatment (0.43 ± 0.82 vs. 1.56 1.34; P &lt; 0.001) follow-up (0.21 0.64 0.45 0.93; = 0.026). improved severity expectoration 0.046) wheezing 0.010) AECB follow-up. The after was group 0.035), particularly terms 0.012) 0.003). risk adverse events, mainly including infectious invasive diseases gastrointestinal symptoms, did not differ two groups (29.6% 30.4%). Conclusion: In CB, reduces ameliorates symptoms. Trail Registration: https://www.chictr.org.cn identifer ChiCTR1900025707.

Language: Английский

Citations

2
Short-term exposure to cigarette smoke upregulates cathepsin S and alters expression of tight junction ZO-1 DOI

Florent Estur,

Émilie Murigneux,

Alexis David

et al.

Biochimie, Journal Year: 2024, Volume and Issue: 226, P. 29 - 34

Published: March 15, 2024

Language: Английский

Citations

1
Inhalation exposure-induced toxicity and disease mediated via mTOR dysregulation DOI Creative Commons

Akshada Shinde,

Jonathan H. Shannahan

Experimental Biology and Medicine, Journal Year: 2024, Volume and Issue: 249

Published: April 22, 2024

Environmental air pollution is a global health concern, associated with multiple respiratory and systemic diseases. Epidemiological supports continued urbanization industrialization increasing the prevalence of inhalation exposures. Exposure to these inhaled pollutants induces toxicity via activation numerous cellular mechanisms including oxidative stress, autophagy, disrupted metabolism, inflammation, tumorigenesis, others contributing disease development. The mechanistic target rapamycin (mTOR) key regulator involved in various processes related modulation metabolism maintenance homeostasis. Dysregulation mTOR occurs following exposures has also been implicated many diseases such as cancer, obesity, cardiovascular disease, diabetes, asthma, neurodegeneration. Moreover, plays fundamental role protein transcription translation inflammatory autoimmune It necessary understand exposure-induced dysregulation since it which may contribute processes. This mini review evaluates available literature regarding several types exposure their impacts on signaling. Particularly we focus signaling pathway outcomes lipid inflammation. Furthermore, will examine implications dysregulated Throughout this review, current gaps be identified enable targeting for development therapeutics.

Language: Английский

Citations

1
The Impact of NO2 on Epithelial Barrier Integrity of a Primary Cell‐Based Air–Liquid Interface Model of the Nasal Respiratory Epithelium DOI Creative Commons
Helena Moratin,

Josephine Lang,

Mellissa Picker

et al.

Journal of Applied Toxicology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

ABSTRACT Nitrogen dioxide (NO 2 ) is a pervasive gaseous air pollutant with well‐documented hazardous effects on health, necessitating precise toxicological characterization. While prior research has primarily focused lower airway structures, the upper airways, serving as first line of defense against airborne substances, remain understudied. This study aimed to investigate functional NO exposure alone or in combination hypoxia secondary stimulus nasal epithelium and elucidate its molecular mechanisms because considered pathophysiological factor onset persistence chronic rhinosinusitis, disease airways. Air–liquid interface cell cultures derived from primary mucosa cells were utilized an vitro model, offering high vitro–in vivo correlation. Our findings demonstrate that induces malfunction epithelial barrier, evidenced by decreased transepithelial electrical resistance increased fluorescein isothiocyanate (FITC)‐dextran permeability. mRNA expression analysis revealed significant increase IL‐6 IL‐8 expressions following . Reduced tight junction component occludin was identified structural correlate damaged barrier. Notably, hypoxic conditions did not alter barrier integrity. These provide information harmful human epithelium, including compromised integrity induction inflammatory responses. Overall, this contributes our understanding underlying also respiratory diseases associated pollution emphasizes importance mitigating emissions safeguard health.

Language: Английский

Citations

1