Silibinin inhibits PM2.5-induced liver triglyceride accumulation through enhancing the function of mitochondrial Complexes I and II DOI Creative Commons
Dexin Li,

Jingxin Zhang,

Yuxin Jin

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 16, 2024

Background The standardized extract of milk thistle seeds, known as silibinin, has been utilized in herbal medicine for over two centuries, with the aim safeguarding liver against deleterious effects various toxic substances. However, role silibinin Particulate Matter (PM2.5)-induced intrahepatic triglyceride accumulation remains unclear. This study seeks to investigate impact on PM2.5-induced and elucidate potential underlying mechanisms. Methods A model was established male C57BL/6J mice through intratracheal instillation PM2.5, followed by assessment weight, body index, measurements triglycerides cholesterol after treatment capsules. Hep G2 cells were exposed PM2.5 suspension create an intracellular model, cell viability, cholesterol, fluorescence staining Nile Red (lipid droplets), DCFH-DA (Reactive Oxygen Species, ROS), well proteomics, real-time PCR, mitochondrial function assays, performed mechanisms involved reducing triglycerides. Results exposure leads accumulation, increased ROS production, elevated expression inflammatory factors, decreased antioxidant downstream genes aryl hydrocarbon receptor. Silibinin can partially or fully reverse these thereby protecting animal livers from damage. In vitro studies show that exerts its protective preserving oxidative phosphorylation complexes I II, particularly significantly enhancing complex II. Succinate dehydrogenase (mitochondrial II) is a direct target but B exhibit different affinities subunits Conclusion improved induced this was, at least part, explained enhancement Complexes

Language: Английский

Glycerol 3-phosphate dehydrogenases (1 and 2) in cancer and other diseases DOI Creative Commons
Sehyun Oh,

Xuan Linh,

Jiwoo Kim

et al.

Experimental & Molecular Medicine, Journal Year: 2024, Volume and Issue: 56(5), P. 1066 - 1079

Published: May 1, 2024

The glycerol 3-phosphate shuttle (GPS) is composed of two different enzymes: cytosolic NAD

Language: Английский

Citations

20
Hydrogen Sulfide: An Emerging Regulator of Oxidative Stress and Cellular Homeostasis—A Comprehensive One-Year Review DOI Creative Commons
Constantin Munteanu, Marius Turnea, Mariana Rotariu

et al.

Antioxidants, Journal Year: 2023, Volume and Issue: 12(9), P. 1737 - 1737

Published: Sept. 7, 2023

Hydrogen sulfide (H2S), traditionally recognized as a toxic gas, has emerged critical regulator in many biological processes, including oxidative stress and cellular homeostasis. This review presents an exhaustive overview of the current understanding H2S its multifaceted role mammalian functioning management. We delve into sources function H2S, mechanisms underlying homeostasis, intricate relationships between these processes. explore evidence from recent experimental clinical studies, unraveling biochemical molecular dictating H2S’s roles modulating responses maintaining The implications therapeutic potential conditions characterized by dysregulation disrupted homeostasis are discussed, highlighting emerging significance health disease. Finally, this underscores challenges, controversies, future directions field, emphasizing need for further research to harness agent diseases associated with homeostatic imbalance. Through review, we aim emphasize pivotal function, encouraging exploration burgeoning area research.

Language: Английский

Citations

35
Novel Multi-Antioxidant Approach for Ischemic Stroke Therapy Targeting the Role of Oxidative Stress DOI Creative Commons
Camilo Briones-Valdivieso,

Felipe Briones,

Sofía Orellana-Urzúa

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(3), P. 501 - 501

Published: Feb. 23, 2024

Stroke is a major contributor to global mortality and disability. While reperfusion essential for preventing neuronal death in the penumbra, it also triggers cerebral ischemia-reperfusion injury, paradoxical injury primarily caused by oxidative stress, inflammation, blood–brain barrier disruption. An burst inflicts marked cellular damage, ranging from alterations mitochondrial function lipid peroxidation activation of intricate signalling pathways that can even lead cell death. Thus, given pivotal role stress mechanisms reinforcement antioxidant defence system has been proposed as protective approach. Although this strategy proven be successful experimental models, its translation into clinical practice yielded inconsistent results. However, should considered availability numerous molecules with wide range chemical properties affect extent injury; several groups molecules, including polyphenols, carotenoids, vitamins, among other compounds, mitigate damage intervening multiple at various stages. Multiple trials have previously conducted evaluate these using melatonin, acetyl-L-carnitine, chrysanthemum extract, edaravone dexborneol, saffron, coenzyme Q10, oleoylethanolamide, treatments. Therefore, multi-antioxidant therapy emerges promising novel therapeutic option due potential synergistic effect provided simultaneous roles individual compounds.

Language: Английский

Citations

14
Comprehensive Management of Stroke: From Mechanisms to Therapeutic Approaches DOI Open Access

Ana Arnalich-Montiel,

Alba Burgos-Santamaría,

Laia Pazó-Sayós

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(10), P. 5252 - 5252

Published: May 11, 2024

Acute ischemic stroke (AIS) is a challenging disease, which needs urgent comprehensive management. Endovascular thrombectomy (EVT), alone or combined with iv thrombolysis, currently the most effective therapy for patients acute (AIS). However, only limited number of are eligible this time-sensitive treatment. Even though there still significant room improvement in management group patients, up until now have been no alternative therapies approved use clinical practice. hope, as research novel emerging generating promising results. These drugs happen to stop palliate some underlying molecular mechanisms involved cerebral ischemia and secondary brain damage. The aim review provide deep understanding these pathogenesis AIS. Later, we will discuss potential that already demonstrated, preclinical studies, improve outcomes

Language: Английский

Citations

7
Association of sensitivity to thyroid hormones with all-cause mortality in euthyroid US adults: a nationwide cohort study DOI Creative Commons

Genfeng Yu,

Si‐Yang Maggie Liu,

Cheng Song

et al.

European Thyroid Journal, Journal Year: 2024, Volume and Issue: 13(1)

Published: Jan. 8, 2024

Background This study aimed to examine the associations of thyroid hormone sensitivity indices, including free triiodothyronine-to-free thyroxine (FT3/FT4) ratio, feedback quantile-based index by FT4 (TFQI ), thyroid-stimulating (TSHI), and thyrotrophic resistance (TT4RI) with all-cause mortality in euthyroid adults. Methods The included 6243 adults from National Health Nutrition Examination Survey (NHANES) 2007–2012. FT3/FT4 TFQI , TSHI, TT4RI were calculated. multivariable Cox proportional hazard regression, restricted cubic spline (RCS), subgroup analysis conducted. Results Individuals fourth quartile (Q4) had lower than those first (Q1) ratio (hazard (HR): 0.70, 95% CI: 0.51, 0.94). Regarding individuals Q4 a 43% higher Q1 (HR: 1.43, 1.05, 1.96) ( P < 0.05, all). Compared participants Q1, no TSHI found. was linearly positively associated mortality. However, showed U-shaped association Conclusions Increased risk for suggesting that increased decreased central hormones. Furthermore, mortality, an inflection point at 0.5. more cohort studies are needed validate conclusions.

Language: Английский

Citations

6
An activated near-infrared mitochondrion-targetable fluorescent probe for rapid detection of NADH DOI

Yaxin Sun,

Yanyun Mao,

Tianwen Bai

et al.

Chemical Communications, Journal Year: 2024, Volume and Issue: 60(46), P. 5932 - 5935

Published: Jan. 1, 2024

A novel NIR fluorescent probe based on quinoline-conjugated benzo[

Language: Английский

Citations

5
Engineered Mitochondrial Transplantation as An Anti-Aging Therapy DOI Creative Commons
Rui Zhao, Chen Dong, Qian Liang

et al.

Aging and Disease, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Aging is an inevitable and complex biological process involving multi-factorial mechanisms. Mitochondrial dysfunction a critical factor in the aging process, characterized by decline mitochondrial quality activity, leading to aging-related diseases. Therefore, mitochondria have become attractive target anti-aging therapies. Several senolytic drugs targeting antioxidant agents been used research past few years. However, these strategies may cause adverse effects with long-term medication. In this extensive review, we propose "mitochondrial transplantation," which transfers healthy from donor cells recipient replace damaged or dysfunctional mitochondria, as new alternative strategy for treating aging-associated introduce contemporary landscape of transplantation, then discuss intensely successful applications transplantation therapy diseases such neurodegenerative diseases, cardiovascular aging, reproductive highlighting its translational potential. Finally, summarize prospect challenges opportunities faces therapy.

Language: Английский

Citations

4
Ionic and Molecular Regulation of Mitochondrial Bioenergetics DOI
David F. Stowe

Published: Jan. 1, 2025

Language: Английский

Citations

0
Remote Organ Damage Induced by Stroke: Molecular Mechanisms and Comprehensive Interventions DOI
Jie Wang, Sen Gao, Yue Cui

et al.

Antioxidants and Redox Signaling, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Significance: Damage after stroke is not only limited to the brain but also often occurs in remote organs, including heart, lung, liver, kidney, digestive tract, and spleen, which are frequently affected by complex pathophysiological changes. The organs human body closely connected, signals transmitted through various molecular substances could regulate changes of organs. Recent Advances: latest studies have shown that inflammatory response plays an important role organ damage stroke, can aggravate activating oxidative stress, sympathetic axis, hypothalamic disturbing immunological homeostasis. Remote cause brain, aggravating damage. Critical Issues: Therefore, in-depth exploration mechanisms adopting corresponding comprehensive intervention strategies become necessary reduce promote protection. Future Directions: strategy involves multifaceted treatment methods such as inflammation regulation, antioxidants, neural stem cell differentiation. It provides a promising alternative for recovery patients inspiration future research treatment. interconnected at level. Only levels we save protect greatest extent. Antioxid. Redox Signal. 00, 000-000.

Language: Английский

Citations

0
Comprehensive transcriptomic analysis integrating bulk and single-cell RNA-seq with machine learning to identify and validate mitochondrial unfolded protein response biomarkers in patients with ischemic stroke DOI Creative Commons
Lu Zhang,

Lei Yue,

Peng Jia

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: April 17, 2025

Ischemic stroke (IS) represents a significant contributor to morbidity and mortality globally. The relationship between IS mitochondrial unfolded protein response (UPRmt) was presently uncertain. This study endeavors explore the fundamental mechanism of UPRmt in by utilizing bioinformatics methods. In GSE58294, differentially expressed genes (DEGs) were obtained, which overlapped with key module -related gene ( -RGs) for producing candidate genes. biomarkers identified from through machine learning, expression validation, receiver operating characteristic (ROC) curves. order verify biomarkers, reverse transcription-quantitative PCR (RT-qPCR) experiments performed on human peripheral blood. Subsequently, predictive nomogram created estimate likelihood developing IS. Next, mechanisms functions related explored enrichment analysis immune infiltration. addition, cells enriched identified, biological processes involved these analyzed intercellular communication virtual knockout experiments. MCEMP1, CACNA1E, CLEC4D as subsequently validated RT-qPCR. RT-qPCR revealed that is most sensitive biomarker. possess strong diagnostic value. Immune infiltration indicated all three are strongly correlated neutrophils. Additionally, single-cell transcriptome data, predominantly Compared sham group, middle cerebral artery occlusion (MCAO) group exhibited enhanced immune-inflammatory responses. Virtual provide preliminary evidence regulatory molecule neutrophil-mediated inflammation, rather than serving merely passive marker. biomarker -RGs, offering new reference diagnosis treatment.

Language: Английский

Citations

0