Green Synthesis of Silver Nanoparticles Using Marine Fungus Cladosporium herbarum: An Assessment of their Antimicrobial Activity DOI Open Access

Manar A. Basheer et al.

Egyptian Journal of Aquatic Biology and Fisheries, Journal Year: 2023, Volume and Issue: 27(5), P. 703 - 718

Published: Sept. 1, 2023

Green, eco-friendly, cost-effective, safe, and reliable processes for synthesizing nanoparticles using microorganisms as bio-nanofactories have garnered significant interest due to their ability transform metals into nanoscale particles use in various fields.In this study, silver nitrate supernatants from Cladosporium herbarum, isolated water samples of Qarun Lake Egypt, were utilized the extracellular biosynthesis (AgNPs), effectiveness against pathogenic was assessed.Silver synthesized four marine fungi, with optimization reaction parameters, resultant characterized a range analytical techniques including Transmission Electron Microscopy (TEM), Fourier infrared (FT-IR), X-ray analysis.The antimicrobial activity biosynthesized evaluated variety pathogens, both Gram-positive Gram-negative bacteria, yeast, fungi.The results demonstrated that by herbarum exhibited potent different pathogens under optimal conditions, substrate concentration 5 mM, an incubation temperature 37°C, pH value 6, 24-hour period.TEM analysis revealed formation spherical, well-dispersed sizes ranging 4 23.1nm, while FT-IR spectroscopy indicated prominent absorption bands at 556, 762, 1349, 1451, 1562, 1620, 3352 cm -1 , corresponding stretching vibrations alkyl halides, C-H groups, phenolic O-H amide C-N NH 2 N-H hydroxyl (OH) respectively.The mediated holds promise potential agent microbes.

Language: Английский

Light-mediated biosynthesis of size-tuned silver nanoparticles using Saccharomyces cerevisiae extract DOI Creative Commons
Lucia Colleselli, Mira Mutschlechner, Martin Spruck

et al.

Bioprocess and Biosystems Engineering, Journal Year: 2024, Volume and Issue: 47(10), P. 1669 - 1682

Published: July 14, 2024

Bio-based production of silver nanoparticles represents a sustainable alternative to commercially applied physicochemical manufacturing approaches and provides qualitatively highly valuable nanomaterials due their narrow size dispersity, high stability biocompatibility with broad application potentials. The intrinsic features depend on shape, whereby the controlled synthesis is challenging necessity. In present study, biosynthesis size-tuned based cell-free extracts Saccharomyces cerevisiae DSM 1333 was investigated. Single parameter optimization strategies in phases cultivation, extraction, were performed modify nanoparticle scale yield. Visible light exploited as tool production. influence white determined by using novel LED systems exposition varying irradiation intensities simultaneous performance control experiments dark. Characterization resulting spectrophotometric analysis, dynamic scattering, scanning electron microscopy, energy dispersive X-ray spectroscopy, revealed spherical controlled, light-mediated shifts markedly increased quantities. Matching irradiated non-irradiated reaction mixtures mirrored enormous functionality photon input sensitivity process. yields more than 90% at

Language: Английский

Citations

1
Ericaria amentacea Algae Extracts: A Sustainable Approach for the Green Synthesis of Silver Oxide Nanoparticles and Their Effectiveness against Leishmaniasis DOI Open Access
Fatouma Mohamed Abdoul‐Latif, Ayoub Ainane,

Ibrahim Houmed Aboubaker

et al.

Processes, Journal Year: 2023, Volume and Issue: 11(11), P. 3227 - 3227

Published: Nov. 15, 2023

In this study, anti-leishmanial activities were performed on silver oxide nanoparticles green synthesized from hexane, ethereal, chloroform, and methanolic extracts of the Ericaria amentacea seaweed. The obtained using a soxhlet extraction system, through simple environmentally friendly method. Physicochemical characterizations, including UV spectrophotometry, transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal gravimetry analysis (TGA), Fourier-transform infrared spectroscopy (FTIR), zeta potential (ZPA), conducted to confirm formation particles. activity was evaluated in vitro MTT assay against Leishmania infantum, tropica, major strains. Additionally, brine shrimp cytotoxicity test Artemia salina larvae assess toxicity products. results showed that significant, with inhibitory concentration values ranging 27.16 μg/mL 38.18 μg/mL. lethal doses higher than 17.08 μg/mL, indicating low toxicity. These findings suggest derived seaweed have applications treatment leishmaniasis. Further research is needed elucidate mechanisms action vivo efficacy these nanoparticles. Moreover, comprehensive studies are necessary before considering their clinical use leishmaniasis treatment.

Language: Английский

Citations

2
Blue Laser-Activated Silver Nanoparticles from Grape Seed Extract for Photodynamic Antimicrobial Therapy Against Escherichia coli and Staphylococcus aureus DOI Open Access
Ahmad Khalil Yaqubi, Suryani Dyah Astuti, Andi Hamim Zaidan

et al.

Journal of lasers in medical sciences, Journal Year: 2023, Volume and Issue: 14, P. e69 - e69

Published: Dec. 29, 2023

Introduction: Living organisms, particularly humans, frequently encounter microorganisms like bacteria, fungi, and viruses in their surroundings. Silver nanoparticles are widely used biomedical devices due to antibacterial, antifungal, antiviral properties. The study evaluates the efficacy of blue laser silver from grape seed extract (AgNPs-GSE) reducing gram-negative Escherichia coli gram-positive Staphylococcus aureus bacteria causing infections. Methods: sample consisted four groups: a control without irradiation (T0), E. samples (A1 A2) irradiated with 405 nm diode at different times concentrations nanoparticles, S. (A3 A4) concentrations. Bacteria groups A2 A4 were treated photosensitizer (PS) made extracts, incubated for 10 minutes, then 90, 120, 150, 180 seconds. cultured on Tryptic Soy Agar (TSA) media, 37 °C, counted by using Quebec colony counter, analyzed ANOVA Tukey tests significance level P<0.05. Results: found that µl AgNPs-GSE, when combined exposure dose 3.44 J/cm², can effectively photoinactivate bacteria. addition AgNPs-GSE led significant reduction viability, 73.93%, 80.96%, 83.80%, respectively. Similarly, seconds adding 1 mM, 1.5 2 mM bacterial viability was reduced 70.87%, 78.04%, 87.01%, Conclusion: findings present indicate an energy density it possible inactivate 83.80% 87.01%.

Language: Английский

Citations

2
Silver/Graphene Oxide Nanostructured Coatings for Modulating the Microbial Susceptibility of Fixation Devices Used in Knee Surgery DOI Open Access

Sorin Constantinescu,

Adelina-Gabriela Niculescu, Ariana Hudiță

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 25(1), P. 246 - 246

Published: Dec. 23, 2023

Exploring silver-based and carbon-based nanomaterials’ excellent intrinsic antipathogenic effects represents an attractive alternative for fabricating anti-infective formulations. Using chemical synthesis protocols, stearate-conjugated silver (Ag@C18) nanoparticles graphene oxide nanosheets (nGOs) were herein obtained investigated in terms of composition microstructure. Scanning electron microscopy (SEM) transmission (TEM) characterizations revealed the formation nanomaterials with desirable physical properties, while X-ray diffraction (XRD) analyses confirmed high purity synthesized nanomaterials. Further, laser-processed Ag@C18-nGO coatings developed, optimized, evaluated biological microbiological outcomes. The highly biocompatible nanostructured proved suitable candidates local modulation biofilm-associated periprosthetic infections.

Language: Английский

Citations

2
Green synthesized silver nanoparticles using Sempervivum tectorum extract have an antibacterial effect against Staphylococcus pseudintermedius isolated from a dog's otitis externa DOI Creative Commons

D. M. Dégi,

Katalin Lányi, F. Beteg

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 20, 2024

Abstract Despite developing a broad spectrum of antibiotics, pathogenic bacteria remain the primary health concern. The emergence drug-resistant strains increased therapeutic failure and mortality. researchers are currently focusing on nanotechnology-based medicines to combat resistance. Our study focuses antimicrobial characteristics plant-mediated silver nanoparticles (Ag NPs). AgNPs were green synthesized (using thermal treatment) reduce nitrate solution (AgNO3) using Sempervivum tectorum L. plant ethanolic aqueous extract. They found be broad-spectrum agents against two Staphylococcus pseudintermedius strains. We investigated AgNPs' antibacterial activity with time-kill curves, MIC, MBC, well diffusion. tests also showed high particles pseudintermedius, minimum inhibitory concentrations ranging between 18.6–90.13 µg/ml. prepared then characterized scanning UV-visible spectroscopy, X-ray diffraction, transmission electronic microscopy (TEM) their morphology crystallinity. Two distinctive absorption peaks AgNP samples observed at 474 nm 487 for leaf extract samples, spherical shape face-centered cubic (FCC) crystalline structure diameter range 10–60 nm, respectively. impact was significantly greater when NPs less than 30 in size. revealed that an may effectively stabilize (AgNPs) various sizes, depending solvent concentration. have shown efficacy both suppressing eradicating microorganisms. facilitated by dual approach involving several processes nanoparticles, such as formation reactive oxygen species presence phytocompounds phenolics proanthocyanidins bound nanoparticle surface. Nanotechnology-based therapies play crucial role treating microbial illnesses veterinary medicine. Significant areas research (NPs) could include studying synergistic interactions Ag doing toxicity profiling, examining stability, investigating bioavailability.

Language: Английский

Citations

0
Production Sericin/Poly(Vinyl Alcohol) Blend Membranes Containing One‐Step Synthesized Silver Nanoparticles by UV Radiation for Obtaining Versatile Antibacterial Materials DOI
Aleyna Temel, Hatice Doğan,

Emine Yıldız

et al.

ChemistrySelect, Journal Year: 2024, Volume and Issue: 9(34)

Published: Sept. 5, 2024

Abstract In this study, silver nanoparticles (AgNPs) were in‐situ synthesized in silk sericin/poly(vinyl alcohol) (SS/PVA) mixtures by an easy one‐step synthesis method with the presence of ultraviolet (UV) radiation. Then, SS/PVA composite membranes containing AgNPs obtained solvent casting method. The chemical structures investigated fourier‐transform infrared spectroscopy (FTIR) and results showed that a mixture sericin PVA. morphological features field emission scanning electron microscopy (FE‐SEM) it was seen have heterogeneous structures. Energy dispersive (EDS) analysis revealed contain carbon, oxygen, nitrogen, sulfur silver. X‐ray diffractometer (XRD) thermogravimetric (TGA) analyses also proved structure membranes. antibacterial activities on Staphylococcus aureus Escherichia coli bacteria disk diffusion test liquid culture medium incubation. It found 5 10 mM quite high activity both types bacteria. functional capacity to be used biomedical applications such as wound care materials.

Language: Английский

Citations

0
Beneficial impact of AgNPs on fucoxanthin production and evaluation of phytochemical variations in Isochrysis sp. DOI

Vijayakumar Maduraimuthu,

Jayappriyan Kothilmozhian Ranishree,

Rathinam Raja

et al.

Algal Research, Journal Year: 2024, Volume and Issue: unknown, P. 103737 - 103737

Published: Oct. 1, 2024

Language: Английский

Citations

0
Phytofabrication, characterization and investigation of biological properties of Punica granatum flower-derived silver nanoparticles DOI
Hamed Barabadi, Faraz Mojab, Salimeh Amidi

et al.

Inorganic Chemistry Communications, Journal Year: 2024, Volume and Issue: 170, P. 113515 - 113515

Published: Nov. 9, 2024

Language: Английский

Citations

0
Photocatalytic performance of sulfur-doped zinc oxide: green synthesis via matoa leaf extract mediation DOI
Ari Sulistyo Rini,

Mohamad Ahmad Syahrul,

Yolanda Rati

et al.

Advances in Natural Sciences Nanoscience and Nanotechnology, Journal Year: 2024, Volume and Issue: 16(1), P. 015008 - 015008

Published: Dec. 31, 2024

Abstract The rapidly expanding industrial sector frequently generates hazardous waste for the environment. Semiconductor materials can be employed to remove contaminants in photocatalyst process. In this paper, sulfur-doped ZnO (at 1%–5% S) has been prepared using matoa leaf extract as a biostabilizer. synthesized sample was characterized XRD, FESEM, EDX, UV–vis, and spectroscopy study its crystallinity, morphology, elemental composition, absorption, functional group. XRD analysis confirmed wurtzite hexagonal-shaped crystal with high purity of without other phases. Dopant S does not significantly affect size crystal. Nanoflakes have changed nanospheres dopant increases. dopants reveal broadening absorption peaks narrow bandgap energy from 2.82–3.08 eV. Sulfur-doped successfully degrades 4-nitrophenol by 98.79% after illumination under UV light 120 min. Therefore, S-doped green synthesis great opportunity applied degrade pollutants found aquatic

Language: Английский

Citations

0
Green Synthesis of Silver Nanoparticles Using Marine Fungus Cladosporium herbarum: An Assessment of their Antimicrobial Activity DOI Open Access

Manar A. Basheer et al.

Egyptian Journal of Aquatic Biology and Fisheries, Journal Year: 2023, Volume and Issue: 27(5), P. 703 - 718

Published: Sept. 1, 2023

Green, eco-friendly, cost-effective, safe, and reliable processes for synthesizing nanoparticles using microorganisms as bio-nanofactories have garnered significant interest due to their ability transform metals into nanoscale particles use in various fields.In this study, silver nitrate supernatants from Cladosporium herbarum, isolated water samples of Qarun Lake Egypt, were utilized the extracellular biosynthesis (AgNPs), effectiveness against pathogenic was assessed.Silver synthesized four marine fungi, with optimization reaction parameters, resultant characterized a range analytical techniques including Transmission Electron Microscopy (TEM), Fourier infrared (FT-IR), X-ray analysis.The antimicrobial activity biosynthesized evaluated variety pathogens, both Gram-positive Gram-negative bacteria, yeast, fungi.The results demonstrated that by herbarum exhibited potent different pathogens under optimal conditions, substrate concentration 5 mM, an incubation temperature 37°C, pH value 6, 24-hour period.TEM analysis revealed formation spherical, well-dispersed sizes ranging 4 23.1nm, while FT-IR spectroscopy indicated prominent absorption bands at 556, 762, 1349, 1451, 1562, 1620, 3352 cm -1 , corresponding stretching vibrations alkyl halides, C-H groups, phenolic O-H amide C-N NH 2 N-H hydroxyl (OH) respectively.The mediated holds promise potential agent microbes.

Language: Английский

Citations

0