Deep Eutectic Solvent-Assisted Carbon Quantum Dots from Biomass Triticum aestivum: A Fluorescent Sensor for Nanomolar Detection of Dual Analytes Mercury (Ⅱ) and Glutathione

Heliyon, Journal Year: 2025, Volume and Issue: 11(2), P. e41853 - e41853

Published: Jan. 1, 2025

Deep eutectic solvents (DESs) have attracted significant attention in recent years due to its environment friendly characteristics and participation the multi-heteroatom doping of carbon quantum dots (CQDs). In this work, we present a simple, fast, environment-friendly microwave synthesis approach for DES-assisted nitrogen chloride co-doped CQDs (N,Cl-CQDs) using choline chloride-urea based DES. A biomass-based precursor, i.e., wheatgrass (Triticum aestivum), has been used as source. Transmission electron microscopy (TEM) showed spherical shape with average 1.75 nm particle diameter prepared CQDs. The surface functionality chemical composition N,Cl-CQDs were determined by X-ray photoelectron spectroscopy (XPS) Fourier transform infrared (FTIR) spectroscopic techniques. obtained high yield (QY), 36 %, compared undoped CQDs, which synthesized an aqueous medium (QY = 15 %). properties such excellent photostability, favorable water solubility, optical stability. sensing platform detection Hg2+ ions GSH LOD value 39 nM 43 nM, respectively. fluorescence quenching mechanism was confirmed several photophysical parameters, lifetime values, radiative rate constant (k r ), non-radiative (knr) others. Furthermore, current system's viability is also tested river sample Hg2+. study exhibited reduced limit broad linear range easy, environmentally friendly, rapid method detecting ions.

Language: Английский

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Emodin, a Potent Anthraquinone Mitigates MPTP-Induced Parkinsons’ Disease Pathology by Regulating Nrf2 and Its Downstream Targets: In Silico and In Vivo Approach

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

Language: Английский

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Green Tea Polyphenol (-)-Epicatechin Pretreatment Mitigates Hepatic Steatosis in an In Vitro MASLD Model

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(8), P. 8981 - 8994

Published: Aug. 16, 2024

Abstract: Metabolic dysfunction-associated steatotic liver disease (MASLD), previously known as non-alcoholic fatty (NAFLD), is becoming more prominent globally due to an increase in the prevalence of obesity, dyslipidemia, and type 2 diabetes. A great deal studies have proposed potential treatments for MASLD, with few them demonstrating promising results. The aim this study was investigate effects (-)-epicatechin (EPI) on development MASLD vitro model using HepG2 cell line by determining metabolic viability cells levels PPARα, PPARγ, GSH. were pretreated 10, 30, 50, 100 μM EPI 4 h assess lipid metabolism. culture established hepatocytes which exposed 1.5 mM oleic acid (OA) 24 h. Moreover, colorimetric MTS assay used order determine cells, PPARα PPARγ protein determined enzyme-linked immunosorbent (ELISA), accumulation visualized Oil Red O Staining method. Also, intracellular glutathione (GSH) measured level oxidative stress. shown treated OA. after incubation OA, significantly decreased, a 71%, compared EPI, where 74–86% respect concentration experiment. Furthermore, GSH exhibited decrease response increasing concentrations. Pretreatment has demonstrated effect vitro. Therefore, considering that mediates metabolism it should be considered hepatoprotective agent future research.

Language: Английский

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Enhanced stability, antioxidant capacity and in vivo anti-inflammatory efficacy of glutathione and quercetin via nanoemulsion formulation

Journal of the Taiwan Institute of Chemical Engineers, Journal Year: 2025, Volume and Issue: 168, P. 105943 - 105943

Published: Jan. 5, 2025

Language: Английский

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Exploring in vitro anticancer potential of ferula gummosa gum on PC-3 cells: insights from cellular and molecular studies

Egyptian Journal of Basic and Applied Sciences, Journal Year: 2025, Volume and Issue: 12(1), P. 86 - 93

Published: Jan. 5, 2025

Ferula gummosa Boiss is a well-known Iranian endemic plant that used to treat inflammation, pain, and infections of the gastrointestinal system. In previous study, we discovered treating PC-3 cells with gum leads significant cytotoxicity induces apoptosis. The purpose this study was examine effects on lactate dehydrogenase activity, reactive oxygen species levels, mitochondrial membrane potential, gene expression patterns. We treated sublethal concentrations IC50 value F. gummosa. This resulted in damage release dehydrogenase. Alongside this, observed decrease intracellular ROS levels weakening potential. These were dose-dependent correlated reduction cellular rhodamine 123 uptake. Our analysis revealed led downregulation BCl-2, CTNNB1 genes, while TP53 BAX upregulated. findings suggest may inhibit cancer through diverse mechanisms. Further research necessary understand precise molecular mechanisms underlying these fully.

Language: Английский

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Protective effects of Pyrogallol and Caffeic acid against Fe2+ -Ascorbate-induced oxidative stress in the wistar rats liver: an in vitro study

Heliyon, Journal Year: 2025, Volume and Issue: 11(3), P. e42518 - e42518

Published: Feb. 1, 2025

Regarding the role of oxidative damage to various tissues in diseases, using antioxidant compounds that protect from is proposed as an important strategy against these diseases. Liver homogenates are frequently employed vitro models for investigating stress owing liver's high metabolic activity and susceptibility damage. In this study, we assessed protective effects two phenolic on Fe2+-ascorbate-induced liver by analyzing markers such lipid peroxidation, protein carbonyl oxidation (PCO), reduced glutathione (GSH), ROS levels. Catechin was used a reference compare with results. The DPPH radical scavenging also evaluated. Our findings demonstrated co-incubation Fe2+-ascorbate system at concentrations (10, 25, 50, 100 μg/mL) led dose-dependent reduction PCO levels, production, GSH depletion. Furthermore, IC50 values free radicals pyrogallol caffeic acid were determined be 76.26 μg/mL 106.31 μg/mL, respectively. Notably, exhibited higher all assays than catechin. most (DPPH, Lipid PCO) (50 μg/mL), antioxidative acid. conclusion, study's results suggest can potentially ameliorate diseases associated stress.

Language: Английский

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Oxalis pes-caprae L. (Oxalidaceae): From Invasive Concern to Promising Bioresource for Health and Sustainable Applications

Plants, Journal Year: 2025, Volume and Issue: 14(4), P. 578 - 578

Published: Feb. 14, 2025

Oxalis pes-caprae L., an invasive plant from South Africa, has developed into a severe ecological threat in many Mediterranean and temperate areas by replacing native flora modifying ecosystems. Although this species detrimental effects on the ecosystem, it unrealized potential as significant bioresource. Current research secondary metabolites found O. pes-caprae, such phenolic acids, oxalates, flavonoids, is summarized review, along with analysis of their biological pharmacological properties, which include antibacterial, antidiabetic, antioxidant activities. could be converted troublesome intruder financially ecologically advantageous bioresource natural products for pharmaceutical, nutraceutical, cosmetic, agricultural applications rethinking resource phytochemical extraction. This would offer novel approach to managing while promoting advancement green technologies sustainable practices.

Language: Английский

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The Potential of Superoxide Dismutase-Rich Tetraselmis chuii as a Promoter of Cellular Health

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1693 - 1693

Published: Feb. 16, 2025

Tetraselmis chuii (T. chuii) is a green, marine, eukaryotic, microalgae that was authorized in the European Union (EU) as novel food for human consumption 2014, and supplement 2017. This narrative review will provide an overview of preclinical clinical trials assessing efficacy T. chuii-derived ingredient, characterized by high superoxide dismutase (SOD) activity (SOD-rich chuii), to improve various aspects cellular health. Collectively, results from vitro, more importantly vivo research, support SOD-rich potential promoter Principally, ingredient appears function indirect antioxidant boosting intracellular systems. Moreover, it can positively modulate inflammatory status up-regulating anti-inflammatory down-regulating pro-inflammatory cytokines factors. In addition, promote health though protecting DNA damage, immune function, strengthening cell structure integrity, modulating signaling pathways. There also some evidence suggest may mitochondrial through up-regulation genes linked biogenesis ATP synthesis. From conducted date, transcriptional activation nuclear factor erythroid 2-related 2 (NRF2) sirtuin 1 (SIRT1) appear be important mediating effects on These exciting preliminary observations represent natural blue with enhance

Language: Английский

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A magnetic nanoreactor with microenvironment regulation capability for targeted and enhanced tumor chemodynamic therapy

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 161356 - 161356

Published: March 1, 2025

Language: Английский

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Targeted Nanoclusters for Intratracheal Delivery in Intraoperative Imaging of Lung Adenocarcinoma

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 3575 - 3594

Published: March 1, 2025

Computed tomography (CT) is widely used all over the world, and detection rate of early lung adenocarcinoma increasing. Minimally invasive thoracic surgery (MITS) has emerged as preferred surgical approach for adenocarcinoma, but identifying small adenocarcinomas difficult. Therefore, there a need non-invasive, convenient efficient way to localize adenocarcinomas. A targeted gold nanocluster intraoperative fluorescence imaging by intratracheal delivery been designed. Au-GSH-anti Napsin nanoclusters (NapA-AuNCs) were synthesized, their physicochemical properties optical characterized. Target effect, cytotoxicity time curve NapA-AuNCs, tested in vivo vitro, was also carried. NapA-AuNCs targeting with red good mucus penetration which had property A549 tissue, very low toxicity normal epithelial cells organs. Intracheal involves faster less accumulation other organs than intravenous administration. successfully conjugated, safe, effective localization.

Language: Английский

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