Human Supplementation with AM3, Spermidine, and Hesperidin Enhances Immune Function, Decreases Biological Age, and Improves Oxidative–Inflammatory State: A Randomized Controlled Trial DOI Creative Commons
Judith Félix, Estefanía Díaz‐Del Cerro, Adriana Baca

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(11), P. 1391 - 1391

Published: Nov. 14, 2024

The positive effect of AM3, spermidine, and hesperidin, which have antioxidant anti-inflammatory properties, on immunity is known, but their the rate aging, known as biological age (BA), unclear. This work aims to test if intake a blend AM3 (150 mg), spermidine (0.6 hesperidin (50 mg) for 2 months could decrease BA improve immunity, redox, inflammatory states. For this, 41 participants (30-63 years) were randomly divided into placebo supplement groups. group took two capsules daily with months, while containing only calcium phosphate talcum powder. Before after treatment, peripheral blood was collected. Immune function assessed in leukocytes, redox state whole-blood cells, erythrocytes, plasma, cytokine concentration both mononuclear cell cultures plasma. Finally, Immunity Clock model applied determine BA. results show that this improves immune functions constitute Clock, decreasing by 11 years reducing oxidative-inflammatory participants. Therefore, can be proposed strategy rejuvenate achieve healthy aging.

Language: Английский

Nebulized Glutathione as a Key Antioxidant for the Treatment of Oxidative Stress in Neurodegenerative Conditions DOI Open Access
João Vitor Lana,

Alexandre Rios,

Renata Takeyama

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(15), P. 2476 - 2476

Published: July 31, 2024

Glutathione (GSH), a tripeptide synthesized intracellularly, serves as pivotal antioxidant, neutralizing reactive oxygen species (ROS) and nitrogen (RNS) while maintaining redox homeostasis detoxifying xenobiotics. Its potent antioxidant properties, particularly attributed to the sulfhydryl group (-SH) in cysteine, are crucial for cellular health across various organelles. The glutathione-glutathione disulfide (GSH-GSSG) cycle is facilitated by enzymes like glutathione peroxidase (GPx) reductase (GR), thus aiding detoxification processes mitigating oxidative damage inflammation. Mitochondria, being primary sources of species, benefit significantly from GSH, which regulates metal supports autophagy, apoptosis, ferroptosis, playing fundamental role neuroprotection. vulnerability brain stress underscores importance GSH neurological disorders regenerative medicine. Nebulization presents novel promising approach delivering this directly central nervous system (CNS), potentially enhancing its bioavailability therapeutic efficacy. This method may offer significant advantages neurodegeneration nuclear factor erythroid 2-related 2 (NRF2) pathway signaling mitochondrial function, thereby providing direct By addressing detrimental effects on neuronal health, nebulized could play managing ameliorating conditions such Parkinson's Disease (PD) Alzheimer's (AD). Further clinical research warranted elucidate potential preserving CNS combating neurodegenerative conditions, aiming improve outcomes individuals affected diseases characterized neuroinflammation.

Language: Английский

Citations

23
Piezoelectric BaTiO3 nanoparticles as oxidase mimics breaking pH limitation for colorimetric detection of glutathione reductase DOI
Jijun Liu, Dan Yang,

Weiling Hu

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 481, P. 148609 - 148609

Published: Jan. 6, 2024

Language: Английский

Citations

11
CAP1: a novel extracellular vesicle marker linked to endothelial senescence in atherosclerosis DOI Creative Commons
Ignacio Hernández, Laura Botana, Javier Díez-Mata

et al.

Biology Direct, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 6, 2025

Endothelial senescence (ES) contributes to aging-related disorders and triggers a senescence-associated secretory-pattern (SASP), releasing Extracellular Vesicles (EVs), potentially impacting atherosclerosis. We used EVs from young (8 weeks) aged (24 months) ApoE-knockout mice detect ES in human aortic (HAEC) coronary (CAEC) endothelial cells. Age-related atherosclerosis was confirmed by increased atheroma plaque formation compared fed high-fat diet, the contribution of on evidenced replicative assay cultured HAEC CAEC, starting with promotion ES. A proteomic analysis depicted recently PCSK9-associated CAP1 protein as cargo component animals highly expressed mouse endarterectomy plaques. Gene silencing inhibited CAEC while overexpressing these cells restored senescent-phenotype. The vivo assessed injecting CAP1-containing isolated into wild-type (WT) either regular or diet. Compared mice, led pronounced along intraluminal Similarly, developed thickened calcified plaques, ß-Gal-positive staining when injected like plaques observed animals. In conclusion, early molecular targets may contribute better management atherosclerosis, which here we unveiled new target.

Language: Английский

Citations

0
Oxidant-Based Cytotoxic Agents During Aging: From Disturbed Energy Metabolism to Chronic Inflammation and Disease Progression DOI Creative Commons

Jürgen Arnhold

Biomolecules, Journal Year: 2025, Volume and Issue: 15(4), P. 547 - 547

Published: April 9, 2025

In humans, aging is an inevitable consequence of diminished growth processes after reaching maturity. The high order biomolecules in cells and tissues continuously disturbed by numerous physical chemical destructive impacts. Host-derived oxidant-based cytotoxic agents (reactive species, transition free metal ions, heme) contribute considerably to this damage. These are under the control immediately acting antagonizing principles, which important ensure cell tissue homeostasis. review, I apply concept host-derived their interplay with principles process. During aging, energy metabolism supply dioxygen nutrients increasingly disturbed. addition, a chronic inflammatory state develops, condition known as inflammaging. balance between protective mechanisms analyzed depending on age-based physiological alterations ATP production. Disturbances associated development age-related diseases comorbidities. An enhanced production reactive species from dysfunctional mitochondria, cellular redox homeostasis, adaptations hypoxia highlighted. Examples how disturbances pathogenesis persons advanced age given.

Language: Английский

Citations

0
Replicative Endothelial Cell Senescence May Lead to Endothelial Dysfunction by Increasing the BH2/BH4 Ratio Induced by Oxidative Stress, Reducing BH4 Availability, and Decreasing the Expression of eNOS DOI Open Access
Ignacio Hernández, Laura Botana, Javier Díez-Mata

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 9890 - 9890

Published: Sept. 13, 2024

Vascular aging is associated with the development of cardiovascular complications, in which endothelial cell senescence (ES) may play a critical role. Nitric oxide (NO) prevents human ES through inhibition oxidative stress, and inflammatory signaling by mechanisms yet to be elucidated. Endothelial cells undergo an irreversible growth arrest alter their functional state after finite number divisions, phenomenon called replicative senescence. We assessed contribution NO during aortic (HAEC) coronary (CAEC) cells, accumulation marker SA-β-Gal was quantified β-galactosidase staining on cultured cells. found negative correlation passaged cultures from P0 P12, between reduction production increased formation reactive oxygen (ROS) nitrogen (ONOO

Language: Английский

Citations

2
Cannabidiol, a Strategy in Aging to Improve Redox State and Immunity in Male Rats DOI Open Access

Mónica De la Fuente,

Noelia Joyera,

Judith Félix

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12288 - 12288

Published: Nov. 15, 2024

Aging is characterized by oxidative stress and immune function impairment, associated with increased morbidity. Cannabidiol (CBD) has anti-oxidant properties, but its role in aging been scarcely studied. This work aims to test the effect of CBD on redox state immunity during rats. In this study, 15-month-old male Long Evans rats received 10 mg/kg b.w/day their diet for weeks were compared same-age control 2-month-old serving as a young group, both following standard diet. After treatment, they sacrificed, spleen, thymus, total blood cells collected. Redox parameters such glutathione reductase peroxidase activities, reduced (GSH) oxidized (GSSG) concentration, GSSG/GSH ratio, lipid peroxidation evaluated. Moreover, functions (chemotaxis, natural killer activity, lymphoproliferation) analyzed spleen. Results show that exhibited immunosenescence However, CBD-treated animals showed higher defenses, lower oxidants cells, better spleen than corresponding age-matched controls. Therefore, administration neutralizes improves immunity, suggesting it strategy achieving healthy aging.

Language: Английский

Citations

2
Redox Homeostasis and Antioxidant Strategies in the Pathophysiology DOI Creative Commons

Alessia Remigante,

Marika Cordaro, Rossana Morabito

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(3), P. 281 - 281

Published: Feb. 26, 2024

The molecular mechanisms underlying oxidative stress, and pathophysiological consequences in cell tissue function, are frequently described as the imbalance between production of reactive species ability to defend through sophisticated antioxidant machinery (Contributions 1–3) [...]

Language: Английский

Citations

2
Expression of a stress-inducible heme oxygenase-1 in NK cells is maintained in the process of human aging DOI Creative Commons
Lucyna Kaszubowska, Jan Jacek Kaczor, Mateusz Jakub Karnia

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: July 19, 2024

Heme oxygenase-1 (HO-1) is a stress-inducible heat shock protein (HSP32) that exerts cytoprotective effects against oxidative stress and inflammation, involved in the maintenance of cellular homeostasis. This study aimed to evaluate expression HO-1 natural killer (NK) cells from individuals different age groups after stimulation with various factors, analyze relationships between concentration this parameters corresponding is, NOD-like receptor 3 (NLRP3), glutathione (GSH), GSH disulfide (GSSG), interleukin 6 (IL-6).

Language: Английский

Citations

1
Consumption of a Probiotic Blend with Vitamin D Improves Immunity, Redox, and Inflammatory State, Decreasing the Rate of Aging—A Pilot Study DOI Creative Commons
Judith Félix, Adriana Baca,

L Uz Taboada

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(11), P. 1360 - 1360

Published: Oct. 25, 2024

There is evidence of the effect probiotic intake on immune system. However, probiotics may have rate aging unknown. The aim this study to determine a blend immunity, redox state, inflammation, and or biological age. A group 10 men 14 women took, daily for 2 months, sachet with three (

Language: Английский

Citations

1
Human Supplementation with AM3, Spermidine, and Hesperidin Enhances Immune Function, Decreases Biological Age, and Improves Oxidative–Inflammatory State: A Randomized Controlled Trial DOI Creative Commons
Judith Félix, Estefanía Díaz‐Del Cerro, Adriana Baca

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(11), P. 1391 - 1391

Published: Nov. 14, 2024

The positive effect of AM3, spermidine, and hesperidin, which have antioxidant anti-inflammatory properties, on immunity is known, but their the rate aging, known as biological age (BA), unclear. This work aims to test if intake a blend AM3 (150 mg), spermidine (0.6 hesperidin (50 mg) for 2 months could decrease BA improve immunity, redox, inflammatory states. For this, 41 participants (30-63 years) were randomly divided into placebo supplement groups. group took two capsules daily with months, while containing only calcium phosphate talcum powder. Before after treatment, peripheral blood was collected. Immune function assessed in leukocytes, redox state whole-blood cells, erythrocytes, plasma, cytokine concentration both mononuclear cell cultures plasma. Finally, Immunity Clock model applied determine BA. results show that this improves immune functions constitute Clock, decreasing by 11 years reducing oxidative-inflammatory participants. Therefore, can be proposed strategy rejuvenate achieve healthy aging.

Language: Английский

Citations

0