The Clinical Use of Myo-Inositol in IVF-ET: A Position Statement from the Experts Group on Inositol in Basic and Clinical Research and on PCOS (EGOI-PCOS), the Polish Society of Andrology, and the International Scientific Association for the Support and Development of Medical Technologies

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(2), P. 558 - 558

Published: Jan. 16, 2025

Background: Myo-inositol plays a vital role in human health, functioning as second messenger of FSH and facilitating the transport glucose into cell. Consequently, myo-inositol is regularly utilized treatment polycystic ovary syndrome (PCOS), wherein it acts upon metabolic factors, improving insulin sensitivity reducing total androgen levels. Patients with PCOS frequently suffer from infertility; thus, use has been explored assistive reproductive technique (ART) procedures. This by no means limited to patients PCOS, inositol found applications non-PCOS patient groups addition male factor infertility. joint statement Experts Group on Inositol Basic Clinical Research (EGOI-PCOS), Polish Society Andrology, International Scientific Association for Support Development Medical Technologies discusses latest evidence this topic, aim interrogating whether could be implemented everyday ART care. Methods: The authors conducted narrative review performed via an independent literature search between July August 2024, using platforms PubMed, Web Science, Google Scholar. Results: In both populations seeking IVF care, MI supplementation prior ovarian stimulation may positively affect gonadotropin duration, oocyte embryo quality, fertilization, clinical pregnancy rates. Conclusions: position recommends that considered potential pretreatment strategy hyperstimulation gonadotropins.

Language: Английский

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BGP-15 improves quality of goat sperm by mitigating oxidative stress during cryopreservation

Cryobiology, Journal Year: 2025, Volume and Issue: 119, P. 105232 - 105232

Published: March 14, 2025

Language: Английский

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SIRT5 Alleviates oxidative Stress of Boar Sperm Induced by Cryopreservation Through IDH2 and SOD2 Pathway

Theriogenology, Journal Year: 2025, Volume and Issue: 241, P. 117424 - 117424

Published: April 7, 2025

Language: Английский

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Boar semen cryopreservation: State of the art, and international trade vision

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 269, P. 107496 - 107496

Published: May 11, 2024

Language: Английский

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Effect of Micronutrients and L-Carnitine as Antioxidant on Sperm Parameters, Genome Integrity, and ICSI Outcomes: Randomized, Double-Blind, and Placebo-Controlled Clinical Trial

Antioxidants, Journal Year: 2023, Volume and Issue: 12(11), P. 1937 - 1937

Published: Oct. 31, 2023

The evaluation of sperm DNA integrity is recommended in the sixth edition 2021 World Health Organization guidelines. Oxidative stress has been identified as a crucial factor leading to genome decay, lipid peroxidation, and nucleoprotein oxidation. This double-blind, placebo-controlled clinical trial aimed assess effect oral antioxidant treatment (Fertilis), which contains L-carnitine some micronutrients, improvement conventional parameters, vitro fertilization/intracytoplasmic injection (IVF/ICSI) outcomes. A total 263 participants were enrolled randomly divided into two groups: 131 received treatment, while 132 placebo. male partners both groups underwent or placebo for duration three months. For each participant, we performed hormonal test, an infectious spermogram, TUNEL assay fragmentation, toluidine blue staining decondensation, IVF/ICSI procedure. Sperm characteristics analysis (volume, count, motility, vitality), decondensation assessed compared results preceding treatment. study outcome revealed significant decrease fragmentation index increase motility after 3 months (p = 0.01 p 0.02, respectively). Additionally, pregnancy rate 0.01) life birth 0.031) was observed. No changes observed parameters vitality) (SDI). Antioxidant therapy beneficial impact on achieving pregnancy, whether through spontaneous conception assisted reproductive procedures (ART).

Language: Английский

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Diverse Biological Functions of Myo-inositol: A Neuro-Metabolite, Osmoprotectant, and Diagnostic Marker

Published: Jan. 1, 2024

Language: Английский

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Towards the Development of Novel, Point-of-Care Assays for Monitoring Different Forms of Antioxidant Activity: The RoXstaTM System

Antioxidants, Journal Year: 2024, Volume and Issue: 13(11), P. 1379 - 1379

Published: Nov. 11, 2024

(1) Background: This study set out to develop a series of simple, novel, rapid methods for assessing different forms antioxidant activity. (2) Methods: An ABTS platform was used engineer: (i) an electrochemical post-activation assay assess free radical scavenging activity; (ii) pre-activation strategy assesses the suppression formation; (iii) horseradish peroxidase-mediated oxidation system monitor hydrogen peroxide activity and (iv) cumene peroxide-hematin determine ability samples scavenge mixture organic peroxides peroxyl alkoxyl radicals generated in presence these reagents. Each assessed against panel candidate compounds their relative activities specificities. In addition, human semen were analyzed how results assays correlated with quality. (3) Results: All 4 revealed dose-dependent on part vitamin C, N-acetyl cysteine, hypotaurine, BSA, melatonin, glutathione, resveratrol epigallocatechin gallate. The other tested either completely lacked or only active one assays. Using unfractionated as exemplar biological fluids rich antioxidants, outputs from individual found reflect aspects When data all combined, accurate predictions reflecting importance oxidative stress defining quality reflected by sperm count, seminal lipid aldehyde content, DNA damage generation mitochondria. (4) Conclusions: methodologies described this paper constitute basis rapid, point-of-care assessments stress.

Language: Английский

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Post-Thaw Storage Temperature Influenced Boar Sperm Quality and Lifespan through Apoptosis and Lipid Peroxidation

Animals, Journal Year: 2023, Volume and Issue: 14(1), P. 87 - 87

Published: Dec. 26, 2023

Cryopreservation deteriorates boar sperm quality and lifespan, which restricts the use of artificial insemination with frozen-thawed semen in field conditions. The objective this study was to test effects post-thaw storage time temperature on survival. Semen ejaculates from five Landrace boars (one ejaculate per boar) were collected frozen following a 0.5 mL-straw protocol. Straws thawed diluted 1:1 (v:v) BTS. samples aliquoted into three parts respectively stored at 5 °C, 17 37 °C for up 6 h. At 0.5, 2, h storage, motility, viability, mitochondrial membrane potential, intracellular reactive oxygen species (ROS) levels apoptotic changes measured. Antioxidant oxidant tested (SPZ) their surrounding environment (SN) each timepoint. results showed significant an impact (total progressive VCL, acrosome integrity), early late changes, MDA SPZ SN. Compared displayed better quality, less levels, lower SN MDA. Notably, extended lifespan without obvious reduction quality. In conclusion, preserves h, facilitates cryopreserved insemination.

Language: Английский

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Positive In Vitro Effect of ROCK Pathway Inhibitor Y-27632 on Qualitative Characteristics of Goat Sperm Stored at Low Temperatures

Animals, Journal Year: 2024, Volume and Issue: 14(10), P. 1441 - 1441

Published: May 12, 2024

Y-27632, as a cytoskeleton protector, is commonly used for low-temperature preservation of cells. Goat sperm are prone to damage the under conditions, leading loss vitality. However, Y-27632 small molecule has not yet been in research on goat semen. This study aims address issue low temperature-induced motility goats by using and explore regulation metabolism. At temperature 4 °C, different concentrations were added diluent. The quality temperature-preserved semen was evaluated detecting motility, antioxidant capacity, mitochondrial activity, cholesterol levels, metabolomics analysis. results indicated that 20 µM significantly increased plasma membrane integrity (

Language: Английский

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Effects of MnTBAP on Porcine Semen Cryopreservation and Capacitation

Antioxidants, Journal Year: 2024, Volume and Issue: 13(6), P. 672 - 672

Published: May 30, 2024

Antioxidants protect cellular function and structure by neutralizing the oxidative stress caused increased reactive oxygen species (ROS) during sperm freezing. Studies on cryopreservation using various antioxidants have demonstrated encouraging results. Many studies used to increase efficiency of freezing improve success rate artificial insemination pregnancy. Manganese (III) tetrakis (4-benzoic acid) porphyrin chloride (MnTBAP) is a newly synthesized antioxidant with positive effects morphology capacitation in humans, rams, stallions. In this study, porcine semen was treated 0, 50, 100, 150 μM MnTBAP based Tris–egg-yolk extender frozen determine whether can assist status cryopreservation. First, motility assessed computer-assisted analysis (CASA) system, 100 treatment group showing highest motile (66.8%) compared that other groups (control, 51.1%; 50 μM, 59.6%); therefore, remaining analyses were conducted comparing two (control vs. group; p < 0.01). Second, fluorescence staining applied examine control microscopy. The viability (41.7% 62.4%) acrosome integrity (77.9% 86.4%) differed significantly (p 0.05). addition, mitochondrial membrane potential (MMP) 46.5% 51.9%; fragmentation rate, estimated Sperm-sus-Halomax kit, 63.4% 57.4%; detected caspase activity 30.1% 22.9%. These tended be higher but did not differ significantly. Third, measurements FACSLyric revealed exhibited state elevated normal lipid arrangement within plasma diminished levels apoptosis ROS We expression genes relevant effectiveness real-time RT-qPCR. Our findings indicated significant alterations mRNA species, exception NOX5 Finally, straws dissolved treat matured denuded oocytes investigate effect fertilization embryo development vitro. cleavage (77.6% 84.1%), blastocyst 9.7% 11.4% conclusion, these results suggest positively affected freeze–thawing, improving capacity, leading development.

Language: Английский

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