Kombucha Versus Vegetal Cellulose for Affordable Mucoadhesive (nano)Formulations

Gels, Journal Year: 2025, Volume and Issue: 11(1), P. 37 - 37

Published: Jan. 4, 2025

Cellulose nanofibers gained increasing interest in the production of medical devices such as mucoadhesive nanohydrogels due to their ability retain moisture (high hydrophilicity), flexibility, superior porosity and durability, biodegradability, non-toxicity, biocompatibility. In this work, we aimed compare suitability selected bacterial vegetal nanocellulose form hydrogels for biomedical applications. The cellulose were synthesized from brewer's spent grains (BSG) kombucha membranes, respectively. Two prepared, one based on other (VNC BNC, respectively). VNC was less opaque more fluid than BNC. cytocompatibility vitro antioxidant activity nanocellulose-based investigated using human gingival fibroblasts (HGF-1, ATCC CRL-2014). investigation hydrogel-mucin interaction revealed that BNC hydrogel had an approx. 2× higher mucin binding efficiency at a hydrogel/mucin ratio (mg/mg) = 4. exhibited highest potential increase number metabolically active viable cells (107.60 ± 0.98% cytotoxicity negative control) among all culture conditions. reduced amount reactive oxygen species (ROS) by about 23% (105.5 2.2% C-) comparison with positive control, whereas ROS level slightly (120.2 3.9% following treatment. Neither two showed antibacterial when assessed diffusion method. data suggest fermentation could be better candidate cytocompatible nanoformulations grains. both VNC, respectively, should improved.

Language: Английский

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The Clothes Matter—Exploiting Agronomical Functions of Trichogenic Selenium Nanoparticles Sharing Activities with Biological Systems Wherein (Were) Formed

Agronomy, Journal Year: 2024, Volume and Issue: 14(1), P. 190 - 190

Published: Jan. 15, 2024

The formation of biogenic selenium nanoparticles (SeNPs) through microbial activities is a promising technique that can contribute to the development reliable, non-toxic and environmentally friendly synthesis methods. Among these, under optimal conditions, myconanotechnology confers particular characteristics due generation bioactive fungal metabolites with various bioactivities. formed SeNPs are known be stabilized by biomolecules microorganism, forming so-called bio-corona or capping structure. composition this greatly impacts activity, but investigations have been limited date. produced Trichoderma sp. potential applications in crops environmental management, as both benefit cultivated plants phytoremediation. This review summarizes biosynthesis contextualizes possible correlations between Trichoderma; it also provides missing analysis could help understand optimize process. Biosynthesis methods probable mechanisms briefly discussed well role trichogenic plant protectants, biostimulants, safe biofortifying agents. knowledge gaps related biosynthesis, control desired for specific agricultural function, technology scale-up connection needed future research directions.

Language: Английский

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Biogenic Selenium Nanoparticles Synthesized Using Alginate Oligosaccharides Attenuate Heat Stress-Induced Impairment of Breast Meat Quality via Regulating Oxidative Stress, Metabolome and Ferroptosis in Broilers

Antioxidants, Journal Year: 2023, Volume and Issue: 12(12), P. 2032 - 2032

Published: Nov. 22, 2023

Selenium (Se) is an indispensable trace element with versatile functions in antioxidant defense poultry. In our previous study, we synthesized a novel type of biogenic selenium nanoparticle based on alginate oligosaccharides (SeNPs-AOS), and found that the particles are sized around 80 nm 8% Se content, dietary addition 5 mg/kg SeNPs-AOS could effectively alleviate deleterious effects heat stress (HS) broilers, but it still unclear whether can improve meat quality. Therefore, aim this study was to evaluate protective breast quality heat-stressed explore relevant mechanisms. Birds at age 21 days were randomly divided into four groups six replicates per group (eight broilers replicate) according 2 × experimental design, using HS (33 ± °C, 10 h/day vs. thermoneutral, TN, under 23 1.5 °C) (5 feed no inclusion) as variables. The results showed decreased cooking loss (p < 0.05), freezing 0.001), shear force 0.01) muscle broilers. non-targeted metabolomics analysis identified 78 differential metabolites between + groups, mainly enriched arginine proline metabolism, β-alanine D-arginine D-ornithine pantothenate, CoA biosynthesis pathways 0.05). Meanwhile, supplementation increased levels total capacity (T-AOC), activities catalase (CAT) glutathione peroxidase (GSH-Px), content malondialdehyde (MDA) 0.05) exposure. Additionally, upregulated mRNA expression CAT, GPX1, GPX3, heme oxygenase-1 (HO-1), masculoaponeurotic fibrosarcoma G (MafG), MafK, selenoprotein W (SELENOW), SELENOK, ferritin heavy polypeptide-1 (FTH1), Ferroportin 1 (Fpn1), nuclear factor erythroid 2-related (Nrf2) while downregulated Kelch-like ECH-associated pro-36 tein (Keap1) prostaglandin-endoperoxide Synthase (PTGS2) HS. These findings demonstrated mitigated HS-induced oxidative damage metabolite changes which may be related regulation Nrf2 signaling pathway synthesis. addition, gene anti-ferroptosis-related molecules HS, suggesting used supplements against

Language: Английский

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Preparation, physicochemical characterization and functional properties of selenium nanoparticles stabilized by polysaccharides from the seeds of Plantago asiatica L

Journal of the Science of Food and Agriculture, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 16, 2025

Selenium nanoparticles (SeNPs), comprising a novel selenium element with higher bioactivity, easily aggregate into large black monomeric particles. In the present study, polysaccharides from seeds of Plantago asiatica L. (PLP) was used as template and morphology/particle size modifier to synthesize nanoparticles. The preparation process SeNPs stabilized by PLP optimized, its antioxidant immunological activities were investigated. optimal conditions PLP-SeNPs reaction temperature 60°C, time 1.5 h, concentration 0.04 mg · mL-1 Na2SeO3/Vc molar ratio 1:5. Stable spherical particle 78.39 ± 2.15 nm prepared through this process. complex at 32 μg demonstrated scavenging against 1,1-diphenyl-2-picrylhydrazyl radicals, hydroxyl radicals 2,2'-azinobis-(3-ethyl-benzothiazolin-6-sulfonic acid) diammonium salt up 49.49 2.58%, 60.99 2.49% 42.07 1.76%, respectively. significantly increased activation RAW264.7 cells improving phagocytosis, reactive oxygen species levels, secretion tumor necrosis factor-α interleukin-10. study lays theoretical foundation for development food-borne exploration their application in functional foods, which will help promote high-value utilization P. L., also has an important guiding significance healthy selenium-enriched food industry. © 2025 Society Chemical Industry.

Language: Английский

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Synthesis, characterizations, properties, modifications and applications of zero-dimensional monoelemental selenium nanomaterials

Coordination Chemistry Reviews, Journal Year: 2025, Volume and Issue: 535, P. 216626 - 216626

Published: March 22, 2025

Language: Английский

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Dual-Stabilized Selenium Nanoparticles with Chitosan and SS31 Peptide: Resolving instability for enhancing ROS elimination, suppressing inflammation, and combating bacterial infections

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: 253, P. 114749 - 114749

Published: May 1, 2025

Language: Английский

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Cytocompatibility, Antimicrobial and Antioxidant Activity of a Mucoadhesive Biopolymeric Hydrogel Embedding Selenium Nanoparticles Phytosynthesized by Sea Buckthorn Leaf Extract

Pharmaceuticals, Journal Year: 2023, Volume and Issue: 17(1), P. 23 - 23

Published: Dec. 22, 2023

Phytosynthesized selenium nanoparticles (SeNPs) are less toxic than the inorganic salts of and show high antioxidant antibacterial activity. Chitosan prevents microbial biofilm formation can also determine dispersal. Never-dried bacterial nanocellulose (NDBNC) is an efficient carrier bioactive compounds a flexible nanofibrillar hydrophilic biopolymer. This study aimed to develop selenium-enriched hydrogel nanoformulation (Se-HNF) based on NDBNC from kombucha fermentation fungal chitosan with embedded biogenic SeNPs phytosynthesized by aqueous extract sea buckthorn leaves (SbLEx)-SeNPsSb-in order both disperse gingival dysbiotic prevent its development. We determined total phenolic content activity SbLEx. Liquid chromatography-mass spectrometry (LC-MS) high-performance liquid chromatography (HPLC) were used for identification polyphenols SeNPsSb characterized transmission electron microscopy-energy-dispersive X-ray spectroscopy (TEM-EDX), dynamic light scattering (DLS), zeta potential, Fourier transform infrared (FTIR) diffraction (XRD) in small- wide-angle (SAXS WAXS). The was SEM, FTIR, XRD, rheology, mucin binding efficiency, contact angle interfacial tension measurements. assessed vitro biocompatibility, antimicrobial antibiofilm potential Se-HNF. TEM, DLS SAXS evidenced polydisperse SeNPsSb, whereas FTIR highlighted heterogeneous biocorona various biocompounds. polar surface smaller (52.82 ± 1.23°) that obtained non-polar (73.85 0.39°). 97.6 0.47 mN/m. efficiency Se-HNF decreased as amount decreased, SEM analysis showed relatively compact structure upon contact. XRD analyses interaction between BNC CS through characteristic peak shifting, rheological measurements pseudoplastic behavior, 0.186 N adhesion force 0.386 energy. results degree cytocompatibility significant

Language: Английский

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Cassava starch‐based film infused with biogenic selenium nanoparticles (bio‐SeNPs) to reduce foodborne pathogens

International Journal of Food Science & Technology, Journal Year: 2024, Volume and Issue: 59(10), P. 7541 - 7549

Published: Sept. 6, 2024

Summary The urgent investigation of antimicrobial films is imperative to mitigate the proliferation pathogens in food, extending product shelf life and ensuring food safety, crucial aspects for public health sustainability global system. We developed cassava starch‐based containing 1% 2% biogenic selenium nanoparticles (Bio‐SeNPs) produced by enterocin MF5 assess activity. addition Bio‐SeNPs altered luminosity gave a reddish hue films. UV–visible, FTIR, thermogram analyses revealed intermolecular interactions between starch Bio‐SeNPs. Scanning electron microscopy showed smooth homogeneous surface nanocomposite Water vapour permeability ranged from 9.4 0.68 g mm h −1 m −2 kPa , lower than control values. Swelling values were higher film Bio‐SeNPs, water transmission was lower. exhibited MICs 5.5 μL mL Listeria spp., 10.9 Salmonella 250 Escherichia coli . Antimicrobial activity 1.5–1.9 log 1.6–2.0 liquid medium dry film, respectively, after 24 h. also applied cherry tomatoes without altering their characteristics compared positive control. Our study demonstrated high potential using biodegradable prevent contamination.

Language: Английский

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Bioactive Hydrogel Formulation Based on Ferulic Acid-Grafted Nano-Chitosan and Bacterial Nanocellulose Enriched with Selenium Nanoparticles from Kombucha Fermentation

Journal of Functional Biomaterials, Journal Year: 2024, Volume and Issue: 15(7), P. 202 - 202

Published: July 22, 2024

Selenium nanoparticles (SeNPs) have specific properties that result from their biosynthesis particularities. Chitosan can prevent pathogenic biofilm development. A wide palette of bacterial nanocellulose (BNC) biological and physical-chemical are known. The aim this study was to develop a hydrogel formulation (SeBNCSFa) based on ferulic acid-grafted chitosan enriched with SeNPs Kombucha fermentation (SeNPsK), which could be used as an adjuvant for oral implant integration other applications. grafted SeBNCSFa were characterized by biochemical methods. cell viability proliferation HGF-1 gingival fibroblasts investigated, well in vitro antioxidant activity. inflammatory response determined enzyme-linked immunosorbent assay (ELISA) the proinflammatory mediators (IL-6, TNF-α, IL-1β) culture medium. Likewise, amount nitric oxide released measured Griess reaction. antimicrobial activity also investigated. grafting degree acid approximately 1.780 ± 0.07% total monomeric units, assuming single-site per monomer. Fourier-transform infrared spectroscopy evidenced convolution BNC spectra, X-ray diffraction analysis highlighted amorphous rearrangement patterns, suggesting multiple interactions. showed high cytocompatibility, enhanced antioxidant, anti-inflammatory, potentials.

Language: Английский

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Functional Properties and Sensory Quality of Kombucha Analogs Based on Herbal Infusions

Antioxidants, Journal Year: 2024, Volume and Issue: 13(10), P. 1191 - 1191

Published: Sept. 30, 2024

Traditionally, kombucha is produced by the fermentation of black or green tea infusions with use SCOBY (Symbiotic Culture Bacteria and Yeasts). However, exhibits ability to ferment other substrates as well, which can be used create novel products new sensory health-promoting properties. This paper investigates antioxidant activity, chemical composition, properties mint, nettle, blackcurrant leaf-based analogs. It has been demonstrated that process significantly influenced (

Language: Английский

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