The Identification of Proteomic Signatures Associated with Alkaline Tolerance in the Skin Mucus of Crucian Carp (Carassius auratus) DOI Open Access
Zhipeng Sun, Jing Huang, Xiao Feng Zhang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11618 - 11618

Published: Oct. 29, 2024

The skin is covered by a protective mucus layer, which essential to the innate defense mechanism of fish. Investigating response various toxic stresses crucial for enhancing its ability tackle environmental challenges and developing strategies mitigate effects. Alkalinity stress assays (50 mmol/L NaHCO3) were conducted on crucian carp (Carassius auratus) from Lake Dali Nur (pH = 9.6) Ping Xiang red freshwater 7) over 7 days. expression mucous proteins was analyzed using liquid chromatography (LC)-spectrometry (MS)/MS Analysis-Data-independent acquisition (DIA) mode. A total 12,537 identified across 20 samples four groups, with 12,025 quantified. In alkaline water population, high alkali resulted in up-regulation 139 down-regulation 500 proteins. contrast, population showed an increase 112 decrease 120; both populations had 23 genes up-regulated 21 down-regulated. protein regulatory network group included 3146 pairwise interactions among 464 nodes, only being differentially expressed Conversely, group’s comprised just 1027 specific 337 6 corresponding common responding extracted visualized populations. Based their relationships levels, these are hypothesized play similar roles under stress. Notably, alpha-globin fragment keratin type I cytoskeletal 13-like markedly expression, increasing nearly thousandfold extremely low level. This suggests it could serve as potential biomarker tolerance, warranting further investigation.

Language: Английский

α-Ketoglutarate modulates the mechanisms of toxicity in crucian carp kidneys chronically exposed to NaHCO3: Metabolomics insights DOI
Wenzhi Liu,

Lin Han,

Fangying Yuan

et al.

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2025, Volume and Issue: 55, P. 101466 - 101466

Published: March 4, 2025

Language: Английский

Citations

0
Study on the physiological responses and tolerance mechanisms to subchronic carbonate alkalinity exposure in the gills of Paramisgurnus dabryanus DOI Creative Commons

Luojia Li,

Wei Luo,

Pengyu Chen

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 287, P. 117319 - 117319

Published: Nov. 1, 2024

Given the reduction of freshwater resources, saline-alkaline aquaculture has emerged as an effective approach to expand fishery's accessible space. High carbonate alkalinity (CA) is a major stressor for aquatic organisms in environments. Paramisgurnus dabryanus potential species culture water, making it ideal model investigating physiological responses and tolerance mechanisms CA exposure fishes. In current study, P. were exposed 15 30 mmol/L NaHCO

Language: Английский

Citations

1
The Identification of Proteomic Signatures Associated with Alkaline Tolerance in the Skin Mucus of Crucian Carp (Carassius auratus) DOI Open Access
Zhipeng Sun, Jing Huang, Xiao Feng Zhang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11618 - 11618

Published: Oct. 29, 2024

The skin is covered by a protective mucus layer, which essential to the innate defense mechanism of fish. Investigating response various toxic stresses crucial for enhancing its ability tackle environmental challenges and developing strategies mitigate effects. Alkalinity stress assays (50 mmol/L NaHCO3) were conducted on crucian carp (Carassius auratus) from Lake Dali Nur (pH = 9.6) Ping Xiang red freshwater 7) over 7 days. expression mucous proteins was analyzed using liquid chromatography (LC)-spectrometry (MS)/MS Analysis-Data-independent acquisition (DIA) mode. A total 12,537 identified across 20 samples four groups, with 12,025 quantified. In alkaline water population, high alkali resulted in up-regulation 139 down-regulation 500 proteins. contrast, population showed an increase 112 decrease 120; both populations had 23 genes up-regulated 21 down-regulated. protein regulatory network group included 3146 pairwise interactions among 464 nodes, only being differentially expressed Conversely, group’s comprised just 1027 specific 337 6 corresponding common responding extracted visualized populations. Based their relationships levels, these are hypothesized play similar roles under stress. Notably, alpha-globin fragment keratin type I cytoskeletal 13-like markedly expression, increasing nearly thousandfold extremely low level. This suggests it could serve as potential biomarker tolerance, warranting further investigation.

Language: Английский

Citations

0