Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 20, 2024
Abstract
This
study
aimed
to
evaluate
the
cytotoxicity,
antioxidant,
and
antimicrobial
activities
of
bacteriocins
produced
by
novel
marine
lactic
acid
bacteria,
specifically
Lactococcus
lactis
NAN6399,
isolated
from
Mediterranean
Sea
coast
Alexandria.
Additionally,
comprehensive
sequencing,
analyses,
protein
identification
this
bacteriocin
was
conducted.
The
results
indicated
that
isolate
has
a
broad
spectrum
against
tested
bacterial
indicators.
Bacteriocin
purified
NAN6399
had
molecular
weight
approximately
20
kDa
designated
as
“lactococcin972”.
Furthermore,
cytotoxicity
antioxidant
were
conducted
on
3(4,5-dimethylthiazol-2-yl),2,5-diphenyl-tetrazolium
bromide
(MTT)
assay
Diphenyl
picrylhydrazyl
(DPPH)
method,
respectively.
showed
“lactococcin972”
exhibited
distinguished
cytotoxic
activity
with
inhibition
rates
80%
breast
cancer
(MCF-7)
30%
lung
(A549).
reduction
72%
DPPH.
These
findings
suggest
lactococcin972
significant
potential
therapeutic
agents
due
their
notable
antimicrobial,
cytotoxic,
properties.
Antioxidants,
Journal Year:
2025,
Volume and Issue:
14(2), P. 203 - 203
Published: Feb. 10, 2025
The
interplay
between
oxidative
stress
and
adipogenesis
is
a
critical
factor
in
the
development
of
obesity
its
associated
metabolic
disorders.
Excessive
reactive
oxygen
species
(ROS)
disrupt
key
transcription
factors
such
as
peroxisome
proliferator-activated
receptor
gamma
(PPARγ)
CCAAT/enhancer-binding
protein
alpha
(C/EBPα),
impairing
lipid
metabolism,
promoting
adipocyte
dysfunction,
exacerbating
inflammation
insulin
resistance.
Antioxidants,
classified
endogenous
(e.g.,
glutathione,
superoxide
dismutase,
catalase)
exogenous
polyphenols,
flavonoids,
vitamins
C
E),
are
pivotal
mitigating
these
effects
by
restoring
redox
balance
preserving
functionality.
Endogenous
antioxidants
neutralize
ROS
safeguard
cellular
structures;
however,
under
heightened
stress,
defenses
often
insufficient,
necessitating
dietary
supplementation.
Exogenous
derived
from
plant-based
sources,
polyphenols
vitamins,
act
through
direct
scavenging,
upregulation
antioxidant
enzymes,
modulation
signaling
pathways
like
nuclear
kappa
B
(NF-κB)
PPARγ,
reducing
peroxidation,
inflammation,
dysfunction.
Furthermore,
they
influence
epigenetic
regulation
transcriptional
networks
to
restore
differentiation
limit
accumulation.
Antioxidant-rich
diets,
including
Mediterranean
diet,
strongly
with
improved
health,
reduced
rates,
enhanced
sensitivity.
Advances
personalized
therapies,
guided
biomarkers
supported
novel
delivery
systems,
present
promising
avenues
for
optimizing
therapeutic
interventions.
This
review,
"Crosstalk
Between
Antioxidants
Adipogenesis:
Mechanistic
Pathways
Their
Role
Metabolic
Health",
highlights
mechanistic
which
regulate
enhance
health.
Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Abstract
Hepatocellular
carcinoma
(HCC)
is
a
life-threatening
disease
with
global
impact,
underscoring
the
urgent
need
for
development
of
new
therapeutic
agents.
This
study
evaluates
effect
selenium-hydroxytyrosol
nanoparticles
(Se-HTNPs)
in
rat
model
HCC
induced
by
diethylnitrosamine
(DEN).
In
vitro,
Se-HTNPs
treatment
reduced
viability
Hep
G2
cells
dose-dependent
manner,
an
IC50
value
61.29
±
1.12
µg/mL.
The
results
confirmed
antioxidant,
anti-inflammatory,
and
anti-carcinogenic
properties
Se-HTNPs,
demonstrating
their
effectiveness
against
DEN-induced
HCC.
effects
were
validated
inhibiting
serum
ALT,
AST,
ALP
enzyme
activities
reducing
total
bilirubin
levels.
Simultaneously,
enhanced
albumin
protein
Additionally,
alleviated
oxidative
stress
significantly
lowering
hepatic
lipid
peroxidation
(MDA)
levels
markedly
increasing
antioxidant
marker
(GSH,
SOD,
TAC)
compared
to
DEN-administered
rats.
also
inflammatory
markers
(TNFα,
IL-6,
IL-1β),
apoptotic
(p53
caspase
3),
VEGF
Furthermore,
DEN
group,
distinctly
suppressed
c-JNK
mRNA
NF-κB
gene
expression
Moreover,
Se-HTNP
improved
histological
alterations
DEN.
conclusion,
these
findings
suggest
that
mitigate
rats
through
potent
properties.
Antioxidants,
Journal Year:
2025,
Volume and Issue:
14(1), P. 115 - 115
Published: Jan. 20, 2025
The
role
of
mitochondria
as
the
electric
engine
cells
is
well
established.
Over
past
two
decades,
accumulating
evidence
has
pointed
out
that,
despite
presence
a
highly
active
glycolytic
pathway
(Warburg
effect),
functional
and
even
upregulated
mitochondrial
respiration
occurs
in
cancer
to
meet
need
high
energy
biosynthetic
demand
sustain
their
anabolic
growth.
Mitochondria
are
also
primary
source
intracellular
ROS.
Cancer
maintain
moderate
levels
ROS
promote
tumorigenesis,
metastasis,
drug
resistance;
indeed,
once
cytotoxicity
threshold
exceeded,
trigger
oxidative
damage,
ultimately
leading
cell
death.
Based
on
this,
metabolic
functions
generation
considered
attractive
targets
synthetic
natural
anticancer
compounds.
Tocotrienols
(TTs),
specifically
δ-
γ-TT
isoforms,
vitamin
E-derived
biomolecules
widely
shown
possess
striking
properties
since
they
regulate
several
molecular
pathways.
Herein,
we
provide
for
first
time
an
overview
reprogramming
redox
homeostasis
perturbation
occurring
cells,
highlighting
involvement
TTs.
This
sheds
light
use
these
compounds
promising
preventive
or
therapeutic
approach
novel
strategies.
Frontiers in Oncology,
Journal Year:
2025,
Volume and Issue:
15
Published: Jan. 23, 2025
Objective
This
study
aimed
to
explore
the
association
between
dietary
antioxidant
index
(DAI)
combined
with
serum
albumin-to-globulin
ratio
(AGR)
and
postoperative
Health-related
quality
of
life
(HRQOL)
in
patients
esophageal
squamous
cell
carcinoma
(ESCC).
Methods
All
were
newly
diagnosed
ESCC
underwent
radical
esophagectomy.
Dietary
data
routine
blood
tests
collected
preoperatively
compute
DAI
AGR.
HRQOL
was
assessed
over
7
years
post-surgery
via
telephone
follow-up
analyzed
longitudinally
using
a
time
deterioration
(TTD)
model.
The
times
compared
log-rank
test,
AGR
examined
through
Cox
regression
models.
Results
A
total
238
included
study.
results
indicate
that
low
DAI-AGR
group,
high
group
had
lower
rate
events,
emotional
functioning
(p=0.014),
dysphagia
(p=0.042),
speech
problems
(p=0.023)
significantly
delayed.
proportional
hazard
model
revealed
preoperative
associated
improvement
(HR=0.575,
95%
CI:
0.368-0.898)
(HR=0.525,
0.298-0.925)
postoperatively,
which
remained
significant
even
after
adjusting
for
covariates.
stratified
analysis
this
demographic
clinical
characteristics.
Conclusions
Our
findings
suggest
is
linked
enhanced
patients,
offering
crucial
insights
practitioners,
researchers.
Theriogenology,
Journal Year:
2025,
Volume and Issue:
236, P. 105 - 113
Published: Feb. 2, 2025
In
vitro
culture
impairs
mitochondrial
metabolism
of
IVP
derived
bovine
embryos
resulting
in
accumulation
reactive
oxygen
species.
Recently,
the
antioxidant
Mito-TEMPO
has
attracted
attention
due
to
its
capability
accumulate
within
mitochondria.
order
investigate
potential
improve
quality
embryos,
this
study
analyzed
developmental
stage
specific
effect
on
capacity,
ROS
balance,
expression
outline
antioxidative
genes
and
cryo-resilience
blastocysts.
three
subsequent
experiments
(1
μM)
was
added
either
maturation
medium
(MTM),
(MTC)
or
both
(MTMC).
Concerning
cleavage-
blastocyst
rates,
no
supplementation
could
be
detected,
although
MTM
and/or
MTC
groups
revealed
significantly
(p
<
0.05)
lower
levels
ROS.
Expression
under
not
affected
group,
whereas
down-regulation
proapoptotic
gene
BAX
observed
as
well
MTMC
groups.
Moreover,
with
functions
mitochondria
(SOD2,
GPX1,
GSTA4)
peroxisomes
(CAT)
group.
contrast,
acting
predominately
outside
(NFE2L2
PRDX1)
affected.
Of
high
impact,
present
for
first
time
greatly
improved
reexpansion
hatching
rates
vitrified-warmed
a
consequence
media.
Collectively,
successfully
proved
that
alleviates
negative
effects
environment
pre-implantation
embryos.
