Recent Advances in the Additive Manufacturing of Stimuli‐Responsive Soft Polymers

Advanced Engineering Materials, Journal Year: 2023, Volume and Issue: 25(21)

Published: Aug. 29, 2023

Stimuli‐responsive polymers (SRPs) are special types of soft materials, which have been extensively used for developing flexible actuators, robots, wearable devices, sensors, self‐expanding structures, and biomedical thanks to their ability change shapes functional properties in response external stimuli including light, humidity, heat, pH, electric field, solvent, magnetic field or combinations two more these stimuli. In recent years, additive manufacturing (AM) aka 3D printing technology SRPs, also known as 4D printing, has gained phenomenal attention different engineering fields, its unique develop complex, personalized, innovative undergo twisting, elongating, swelling, rolling, shrinking, bending, spiraling, other complex morphological transformations. Herein, an effort made provide insightful information about the AM techniques, type applications including, but not limited tissue engineering, bionics, construction, smart textiles. This article incorporates current challenges prospects, hoping basis utilization this fields. It is expected that amalgamation with SRPs would unparalleled advantages arenas.

Language: Английский

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Advancements in tissue and organ 3D bioprinting: Current techniques, applications, and future perspectives

Materials & Design, Journal Year: 2024, Volume and Issue: 240, P. 112853 - 112853

Published: March 19, 2024

3D bioprinting techniques have emerged as a flexible tool in tissue engineering and regenerative medicine to fabricate or pattern functional bio-structures with precise geometric designs, bridging the divergence between engineered natural constructs. A significantly increasing development has been achieved understanding relationship 3D-printing process structures, properties, applications of objects created. The ongoing advancement novel biomaterial inks enabled manufacturing models vitro implants capable achieving some level success preclinical trials. Remarkable progress cell biology biology-inspired computational design assisted latest milestone planned tissue- organ-like constructs having specific levels functionality. However, biofabricated still long way go before reaching clinics. This review presents picture context medicine, focus on biomaterials-related design-centred aspects. Biomedical are described detail relation major tissues organs considered human body. Current technical limitations, challenges, future prospects improvements critically outlined discussed.

Language: Английский

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Numerical modeling of oxygen diffusion in tissue spheroids undergoing fusion using function representation and finite volumes

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 11, 2025

Abstract A three-dimensional cell culture called a spheroid serves as foundational entity in wide variety of modern tissue engineering applications, including 3D-bioprinting and preclinical drug testing. Lack oxygen within spheroids hinders metabolism cells eventually leads to death. Prevention necrosis is crucial success methods such prevention requires estimation viability the spheroid. We propose novel approach for numerical modeling diffusion during their fusion. The based on solutions partial differential equations application Function Representation (FRep) framework geometric modeling. present meshes derived from geometry fusing spheroids, method selecting optimal size, several statistics estimating cellular viability. Our findings provide insights into cultures thus improving robustness biotechnological that employ spheroids.

Language: Английский

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Exploring the interaction between extracellular matrix components in a 3D organoid disease model to replicate the pathophysiology of breast cancer

Journal of Experimental & Clinical Cancer Research, Journal Year: 2023, Volume and Issue: 42(1)

Published: Dec. 16, 2023

In vitro models are necessary to study the pathophysiology of disease and development effective, tailored treatment methods owing complexity heterogeneity breast cancer large population affected by it. The cellular connections tumor microenvironments observed in vivo often not recapitulated conventional two-dimensional (2D) cell cultures. Therefore, developing 3D that mimic complex architecture physiological circumstances tumors is crucial for advancing our understanding illness. A scaffold-free model mimics allowing cells self-assemble/pattern into structures, contrast with other rely on artificial scaffolds. It possible this model, whether applied using patient-derived primary (fibroblasts, endothelial cells, cells), can accurately replicate heterogeneity. complicated interactions between different types modelled integrating critical components microenvironment, such as extracellular matrix, vascular growth factors. Tissue interactions, immune infiltration, effects milieu drug resistance be studied model. mimicking a useful tool studying molecular basis disease, identifying new therapeutic targets, evaluating modalities. provides more physiologically appropriate high-throughput platform screening compound library 96-384 well format. We critically discussed rapid personalized strategies accelerated platforms close gap traditional 2D culture investigations.

Language: Английский

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Endothelial dysfunction and cardiovascular diseases: The role of human induced pluripotent stem cells and tissue engineering

Journal of Biomedical Materials Research Part A, Journal Year: 2024, Volume and Issue: 112(8), P. 1286 - 1304

Published: Jan. 17, 2024

Cardiovascular disease (CVD) remains to be the leading cause of death globally today and therefore need for development novel therapies has become increasingly important in cardiovascular field. The mechanism(s) behind pathophysiology CVD have been laboriously investigated both stem cell bioengineering laboratories. Scientific breakthroughs paved way better mimic types interest recent years, with ability generate any type from reprogrammed human pluripotent cells. Mimicking native extracellular matrix using organic inorganic biomaterials allowed full organs recapitulated vitro. In this paper, we will review techniques biology which fruitfully combined fueled advances We provide a brief introduction CVD, reviewing some studies as related role endothelial cells dysfunction. Recent widely used discussed, providing broad overview collaboration between these two fields their overall impact on tissue engineering devices implications treatment disease.

Language: Английский

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Technology for the formation of engineered microvascular network models and their biomedical applications

Nano Convergence, Journal Year: 2024, Volume and Issue: 11(1)

Published: March 2, 2024

Abstract Tissue engineering and regenerative medicine have made great progress in recent decades, as the fields of bioengineering, materials science, stem cell biology converged, allowing tissue engineers to replicate structure function various levels vascular tree. Nonetheless, lack a fully functional system efficiently supply oxygen nutrients has hindered clinical application bioengineered tissues for transplantation. To investigate biology, drug transport, disease progression, vascularization engineered medicine, we analyzed different approaches designing microvascular networks create models. This review discusses advances field engineering, explores potential future challenges, offers methodological recommendations.

Language: Английский

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Three-Dimensional Bioprinting: A Comprehensive Review for Applications in Tissue Engineering and Regenerative Medicine

Bioengineering, Journal Year: 2024, Volume and Issue: 11(8), P. 777 - 777

Published: July 31, 2024

Since three-dimensional (3D) bioprinting has emerged, it continuously to evolved as a revolutionary technology in surgery, offering new paradigms for reconstructive and regenerative medical applications. This review highlights the integration of 3D printing, specifically bioprinting, across several surgical disciplines over last five years. The methods employed encompass recent literature focusing on innovations applications 3D-bioprinted tissues and/or organs. findings reveal significant advances creation complex, customized, multi-tissue constructs that mimic natural tissue characteristics, which are crucial interventions patient-specific treatments. Despite technological advances, paper introduces discusses challenges remain, such vascularization bioprinted tissues, with host tissue, long-term viability concludes while holds substantial promise transforming practices enhancing patient outcomes, ongoing research, development, clear regulatory framework essential fully realize potential future clinical

Language: Английский

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Three-Dimensional Bioprinting in Soft Tissue Engineering for Plastic and Reconstructive Surgery

Bioengineering, Journal Year: 2023, Volume and Issue: 10(10), P. 1232 - 1232

Published: Oct. 21, 2023

Skeletal muscle tissue engineering (TE) and adipose have undergone significant progress in recent years. This review focuses on the key findings these areas, particularly highlighting integration of 3D bioprinting techniques to overcome challenges enhance regeneration. In skeletal TE, enables precise replication architecture. addresses need for parallel alignment cells proper innervation. Satellite (SCs) mesenchymal stem (MSCs) been utilized, along with co-cultivation strategies vascularization Therefore, various printing methods materials, including decellularized extracellular matrix (dECM), explored. Similarly, engineering, has employed challenge vascularization; addressing this is vital graft survival. Decellularized biomimetic scaffolds used as biological inks, adipose-derived (ADSCs), The dECM alginate bioinks demonstrated improved adipocyte maturation differentiation. These highlight potential engineering. By integrating specific cell types, biomaterials, methods, made However, such fabricating larger constructs, translating human models, obtaining regulatory approvals cellular therapies remain be addressed. Nonetheless, advancements underscore transformative impact research its future clinical applications.

Language: Английский

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4D printed therapeutic systems

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 265 - 281

Published: Jan. 1, 2025

Language: Английский

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Sheet-based extrusion bioprinting: a new multi-material paradigm providing mid-extrusion micropatterning control for microvascular applications

Biofabrication, Journal Year: 2024, Volume and Issue: 16(2), P. 025032 - 025032

Published: March 6, 2024

Abstract As bioprinting advances into clinical relevance with patient-specific tissue and organ constructs, it must be capable of multi-material fabrication at high resolutions to accurately mimick the complex structures found in body. One most fundamental regenerative medicine is microvasculature. Its continuous hierarchical branching vessel networks bridge surgically manipulatable arteries (∼1–6 mm) capillary beds (∼10 µ m). Microvascular perfusion established quickly for autologous, allogeneic, or engineered grafts survive implantation heal place. However, traditional syringe-based techniques have struggled produce perfusable constructs resolution arterioles (∼100-10 m) microvascular tissues. This study introduces novel CEVIC device (i.e. C ontinuously E xtruded V ariable I nternal hanneling), a technology that breaks current extrusion-based paradigm pushing cell-laden hydrogels through nozzle as filaments, instead, version explored here, extruding thin, wide hydrogel sheets. The adapts chaotic printing approach control width number microchannels within construct extruded on-the-fly). Utilizing flow valve designs, this strategy can gradients varying geometry materials across channels average widths ranging from 621.5 ± 42.92% m 11.67 14.99% m, respectively, encompassing range vessels. These also include fugitive/sacrificial ink vacates leave demonstrably channels. In proof-of-concept experiment, co-culture two cell types, endothelial cells pericytes, sustained over 90% viability throughout 1 week CEVIC-produced gelatin methacryloyl-sodium alginate constructs. results justify further exploration generating CEVIC-bioprinted microvasculature, such pre-culturing studies.

Language: Английский

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