Cell Proliferation,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 20, 2024
Osteoarthritis
(OA),
a
joint
disease,
burdens
global
healthcare
due
to
aging
and
obesity.
Recent
studies
show
that
extracellular
vesicles
(EVs)
from
bone
marrow-derived
mesenchymal
stem
cells
(BMSCs)
contribute
homeostasis
OA
management.
However,
the
impact
of
donor
age
on
BMSC-derived
EV
efficacy
remains
underexplored.
In
this
study,
we
investigated
young
BMSCs
(2-month-old)
in
mitigating
OA,
contrasting
them
with
EVs
aged
(27-month-old).
The
study
used
destabilisation
medial
meniscus
(DMM)
surgery
mouse
knee
joints
induce
accelerated
OA.
Cartilage
degeneration
markers
senescence
markers'
expression
levels
were
response
treatment.
therapeutic
chondrocytes
under
inflammatory
responses
was
also
evaluated.
Despite
having
similar
morphologies,
markedly
decreased
improved
chondroprotection
by
activating
PTEN
pathway
while
simultaneously
suppressing
upregulation
PI3K/AKT
pathways,
proving
be
more
effective
than
those
older
vitro.
Furthermore,
intraperitoneal
injections
donors
significantly
mitigated
progression
preserving
cartilage
reducing
synovitis
surgical
model
using
DMM
mice.
These
findings
highlight
as
critical
determinant
potential
for
clinical
use
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(13), P. 7411 - 7411
Published: July 5, 2024
Advancing
age
is
associated
with
several
age-related
diseases
(ARDs),
musculoskeletal
conditions
impacting
millions
of
elderly
people
worldwide.
With
orthopedic
contributing
towards
considerable
number
patients,
a
deeper
understanding
bone
aging
the
need
hour.
One
underlying
factors
cellular
senescence
and
its
secretory
phenotype
(SASP).
SASP
comprises
pro-inflammatory
markers,
cytokines
chemokines
that
arrest
cell
growth
development.
The
accumulation
over
years
leads
to
chronic
low-grade
inflammation
advancing
age,
also
known
as
inflammaging.
pathways
molecular
mechanisms
focused
on
inflammaging
are
currently
limited
but
increasingly
being
explored.
Most
genes,
involved
in
coincide
those
cancer
other
ARDs
like
osteoarthritis
(OA).
Thus,
exploring
these
using
techniques
sequencing,
identifying
combatting
them
most
suitable
approach
crucial
for
healthy
early
detection
ARDs.
Several
approaches
can
be
used
aid
regeneration
reduce
bone.
These
may
pharmacological,
non-pharmacological
lifestyle
interventions.
increasing
evidence
intricate
relationship
between
aging,
senescence,
ARDs,
anti-aging
strategies
marrow
(BM).
OBM Geriatrics,
Journal Year:
2025,
Volume and Issue:
09(02), P. 1 - 32
Published: April 14, 2025
Cellular
senescence
(aging)
is
a
physiological
process
that
plays
role
in
tissue
remodeling,
wound
healing,
and
embryogenesis.
Prolonged
of
cells
can
have
detrimental
effects
trigger
secretory
phenotype
(SASP,
senescence-associated
phenotype),
degenerative
disorders,
cancer,
age-related
diseases.
Suitable
biomarkers
range
different
laboratory
methods
are
used
to
investigate
these
complex
relationships
vitro
vivo.
Since
universal
biomarker
for
cell
has
not
yet
been
identified,
numerous
identify
senescent
cell.
The
detection
quantification
their
SASP
provide
the
basis
targeted
treatment
patient.
In
parallel,
single-cell
analysis
also
required
quantitative
assessment
therapy
result.
Depending
on
facilities
performing
analysis,
wide
available.
this
review,
we
general
overview
accessible
techniques
such
as
immunohistochemistry
using
microscopy
automated
flow
cytometry
introduce
new
possibilities
by
modern
like
mass
spectrometry
or
genetic
method
single
cells.
focus
here
use
routine
laboratories.
classical
with
enzyme
immunoassays,
measurement
products
(IL-6,
IL-8),
part
work.
This
review
discusses
ideas
visualisation
clinical
patient
data
gerontology.
An
outlook
potential
future
optimization
improve
rejuvenate
status
patients
cellular
organ-specific
level
discussed.
Knee Surgery Sports Traumatology Arthroscopy,
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 6, 2024
Abstract
Purpose
Chondrocyte‐based
cell
therapies
are
effective
for
the
treatment
of
chondral
lesions,
but
remain
poorly
indicated
diffuse
lesions
in
context
early
osteoarthritis
(OA).
The
aim
this
study
was
to
develop
a
protocol
obtain
chondroprogenitor
cells
suitable
within
OA.
Methods
Cartilage
were
expanded
at
low
density
human
platelet
lysate
(hPL).
A
test
performed
exclude
senescence.
expression
surface
cluster
differentiation
146,
166,
major
histocompatibility
complex
(MHC)‐I
and
MHC‐II
genes
interest
evaluated,
as
well
trophic
potential
these
cells,
by
assessment
lubricin
matrix
production.
immunomodulatory
assessed
through
their
co‐culture
with
macrophages.
Results
hPL
showed
higher
proliferation
rate
than
standard‐density
no
replicative
senescence,
immunogenicity
lubricin.
Moreover,
they
presented
an
increased
chondrogenic
antihypertrophic
markers,
superior
deposition
if
compared
cultured
standard
density.
induced
on
macrophages
upregulation
CD206,
although
increase
CD163
observed
presence
low‐density
cells.
Conclusions
These
findings
lay
grounds
explore
clinical
usefulness
cartilage
treat
OA
joints
both
autologous
allogenic
use.
Level
Evidence
Not
applicable.
Biology,
Journal Year:
2023,
Volume and Issue:
12(10), P. 1301 - 1301
Published: Oct. 2, 2023
Cellular
senescence
is
a
state
of
irreversible
growth
arrest
with
profound
phenotypic
changes,
including
the
senescence-associated
secretory
phenotype
(SASP).
Senescent
cell
accumulation
contributes
to
aging
and
many
pathologies
chronic
inflammation,
type
2
diabetes,
cancer,
neurodegeneration.
Targeted
removal
senescent
cells
in
preclinical
models
promotes
health
longevity,
suggesting
that
selective
elimination
promising
therapeutic
approach
for
mitigating
myriad
age-related
humans.
However,
moving
senescence-targeting
drugs
(senotherapeutics)
into
clinic
will
require
targets
biomarkers,
fueled
by
an
improved
understanding
complex
dynamic
biology
populations
their
molecular
profiles,
as
well
mechanisms
underlying
emergence
maintenance
SASP.
Advances
mass
spectrometry-based
proteomic
technologies
workflows
have
potential
address
these
needs.
Here,
we
review
translational
research
how
approaches
added
our
knowledge
date.
Further,
lay
out
roadmap
from
fundamental
biological
discovery
clinical
translation
senotherapeutic
through
development
application
emerging
technologies,
targeted
untargeted
approaches,
bottom-up
top-down
methods,
stability
proteomics,
surfaceomics.
These
are
integral
probing
cellular
composition
dynamics
and,
ultimately,
senotype-specific
biomarkers
senotherapeutics
(senolytics
senomorphics).
This
aims
highlight
areas
applications
proteomics
aid
exploring
new
future
senotherapeutics.
Marine Drugs,
Journal Year:
2024,
Volume and Issue:
22(11), P. 496 - 496
Published: Nov. 4, 2024
Aging
is
closely
linked
to
various
health
challenges,
including
cardiovascular
disease,
metabolic
disorders,
and
neurodegenerative
conditions.
This
study
emphasizes
the
critical
role
of
bioactive
compounds
derived
from
marine
sources,
such
as
antioxidants,
omega-3
fatty
acids,
vitamins,
minerals,
polysaccharides,
in
addressing
oxidative
stress,
inflammation,
disorders
related
aging.
Incorporating
these
materials
into
functional
foods
not
only
provides
essential
nutrients
but
also
delivers
therapeutic
effects,
thereby
promoting
healthy
aging
mitigating
age-related
diseases.
The
growth
global
anti-aging
market,
particularly
North
America,
Europe,
Asia,
underscores
significance
this
study.
review
systematically
analyzes
current
research,
identifying
key
compounds,
their
mechanisms
action,
potential
benefits,
thus
highlighting
broad
applicability
marine-derived
enhancing
improving
quality
life
populations.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: March 28, 2024
Abstract
Platelet-rich
plasma
(PRP)
therapy
has
emerged
as
a
potential
treatment
option
for
osteoarthritis
(OA)
due
to
its
ability
promote
tissue
healing
and
anti-inflammatory
effects.
More
evidences
are
needed
establish
the
optimal
protocol.
We
present
here
retrospective
analysis
of
252
patients
treated
with
PRP
big
joints
OA
between
2020
2022.
aimed
evaluate
benefits
combined
rehabilitation
on
pain
function
well
prognosis
factors.
observed
clinically
significant
improvements
in
(VAS
improvement
49%
at
6
months,
45%
12
months)
(Single
Assessment
Numeric
Evaluation
i.e.
SANE
score
44%
39%
months).
Multiple
shoots
high
sports
activity,
especially
competition
level,
were
found
favorable
The
authors
would
suggest
offering
systematically
practitioners.
Moreover,
that
multiple
shoots,
spaced
from
3–4
weeks,
total
platelet
count
not
exceeding
3*10
9
platlets
per
shoot
could
be
viable
OA.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: April 1, 2024
Abstract
Platelet-rich
plasma
(PRP)
is
a
biological
blood-derived
therapeutic
that
releases
several
proteins
in
injured
tissue,
regulating
the
inflammatory
process
and
triggering
tissue
anabolism.
Outside
of
central
nervous
system
(CNS),
these
platelets
represent
major
source
brain-derived
neurotrophic
factor
(BDNF).
The
associated
anti-inflammatory,
antiapoptotic,
antioxidant
properties
this
neurotrophin
could
have
potential
applicability
applied
pathophysiology
osteoarthritis
(OA).
In
osteoarthritic
joints,
it
suggested
synovial
cells
release
BDNF,
being
found
higher
concentrations
protein
patient’s
fluid.
Thus,
considering
as
an
important
BDNF
role
regulate
joint
homeostasis
OA
outcomes,
study
aimed
to
evaluate
contribution
PRP
repair
activities
on
experimental
model
(EOA)
induced
by
monoiodine
acetate
(MIA)
insult.
Initially,
characterization
was
performed,
obtaining
leukocyte-poor-platelet-rich
(LP-PRP).
After,
vitro
analysis
indicated
activated
Ca
2+
suppression
M1
macrophage
polarization
LP-PRP
dependent
BDNF-TrkB
receptor.
behavioral
ex-vivo
analyses
EOA
animals
showed
functional
recovery
gait
parameters
mitigation
damage
LP-PRP,
such
downregulation
cytokines
(TNF-α),
reduction
pain
(OARSI
score
neuronal
injury
through
ATF-3
[+]
cells),
depends
Trk-B
results
indicate
receptor
appear
be
essential
mechanisms
with
implications
well.
These
findings
contribute
clarifying
homeostasis,
repurposing
its
restricted
CNS
functions
non-neuronal
tissues
option
for
chronic
management
