Quantitative measurements of reactive oxygen species partitioning in electron transfer flavoenzyme magnetic field sensing

Frontiers in Physiology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 2, 2024

Biological magnetic field sensing that gives rise to physiological responses is of considerable importance in quantum biology. The radical pair mechanism (RPM) a fundamental process can explain some the observed biological effects. In magnetically sensitive (RP) reactions, coherent spin dynamics between singlet and triplet pairs are modulated by weak fields. resulting reaction products lead distinct signaling channels cellular outcomes. A prevalent RP biology flavin semiquinone superoxide (O 2 •− ) activation molecular oxygen. This result partitioning reactive oxygen species (ROS) form either O or hydrogen peroxide (H ). Here, we examine recombinant human electron transfer flavoenzyme (ETF) reoxidation selectively measuring H product distributions. ROS was two static fields at 20 nT 50 μT, with 13% decrease 10% increase relative µT. RPM yields were calculated for realistic flavin/superoxide across range fields, agreement experimental results. For born RP, also predicts about three times more than , results exhibiting four time produced ETF. method presented here illustrates potential novel flavoprotein sensor directly linked mitochondria bioenergetics be used as target study cell physiology.

Language: Английский

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Cellular and Molecular Effects of Magnetic Fields

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8973 - 8973

Published: Aug. 17, 2024

Recently, magnetic fields (MFs) have received major attention due to their potential therapeutic applications and biological effects. This review provides a comprehensive analysis of the cellular molecular impacts MFs, with focus on both in vitro vivo studies. We investigate mechanisms by which MFs influence cell behavior, including modifications gene expression, protein synthesis, signaling pathways. The interaction components such as ion channels, membranes, cytoskeleton is analyzed, along effects processes like proliferation, differentiation, apoptosis. Molecular insights are offered into how modulate oxidative stress inflammatory responses, pivotal various pathological conditions. Furthermore, we explore regenerative medicine, cancer treatment, neurodegenerative diseases. By synthesizing current findings, this article aims elucidate complex bioeffects thereby facilitating optimized application medical biotechnological fields.

Language: Английский

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Electro‐ and Magneto‐Active Biomaterials for Diabetic Tissue Repair: Advantages and Applications

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

Abstract The diabetic tissue repair process is frequently hindered by persistent inflammation, infection risks, and a compromised microenvironment, which lead to delayed wound healing significantly impact the quality of life for patients. Electromagnetic biomaterials offer promising solution enabling intelligent detection wounds through electric magnetic effects, while simultaneously improving pathological microenvironment reducing oxidative stress, modulating immune responses, exhibiting antibacterial action. Additionally, these materials inherently promote regeneration regulating cellular behavior facilitating vascular neural repair. Compared traditional biomaterials, electromagnetic provide advantages such as noninvasiveness, deep penetration, responsiveness, multi‐stimuli synergy, demonstrating significant potential overcome challenges This review comprehensively examines superiority in repair, elucidates underlying biological mechanisms, discusses specific design strategies applications tailored characteristics wounds, with focus on skin bone defect By addressing current limitations pursuing multi‐faceted strategies, hold improve clinical outcomes enhance

Language: Английский

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Pulsed electromagnetic fields treatment ameliorates cardiac function after myocardial infarction in mice and pigs

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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Synergistic Responses Conferred by Concurrent Optical and Magnetic Stimulation are Attenuated by Simultaneous Exposure to Streptomycin: An Aminoglycoside Antibiotic Dilemma

Published: June 7, 2024

Concurrent Optical and Magnetic Stimulation (COMS) combines extremely low frequency electromagnetic light stimulation modalities for enhanced wound healing. In this report, we investigated the potential mechanistic synergism between magnetic components by comparing their individual combined cellular responses. Although each mode was capable of stimulating cell proliferation, fields exposure alone produced greater proliferation enhancement than alone, yet effects were supra-additive Reactive oxygen species incrementally reduced to light, magnetics combination, where statistical significance ultimately achieved. On other hand, ATP production significantly in response or combination with light. These results suggest that mitochondrial respiratory efficiency preferentially improved two stimuli. Protein expression related combinatory COMS modality as protein levels TRPC1 cation channel previously implicated part a calcium-mitochondrial signaling axis invoked involved proliferation. corroborate facilitates functional impinge on mitochondria-dependent developmental The aminoglycoside antibiotics (AGAs) have been shown antagonize TRPC1-mediated magnetotransduction. Streptomycin applied during any modes enhancement, whereas streptomycin added after did not. Notably, field partially overcoming antagonism Transductional combinatorial photon-electromagnetic thus aligns previous studies showing AGAs block magnetotransduction when exposure. prophylactic usage (neomycin) should be avoided therapy maintain clinical efficacy would common concern most regenerative paradigms.

Language: Английский

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Synergistic Cellular Responses Conferred by Concurrent Optical and Magnetic Stimulation Are Attenuated by Simultaneous Exposure to Streptomycin: An Antibiotic Dilemma

Bioengineering, Journal Year: 2024, Volume and Issue: 11(7), P. 637 - 637

Published: June 21, 2024

Concurrent optical and magnetic stimulation (COMS) combines extremely low-frequency electromagnetic light exposure for enhanced wound healing. We investigated the potential mechanistic synergism between components of COMS by comparing their individual combined cellular responses. Lone field produced greater enhancements in cell proliferation than alone, yet effects fields were supra-additive Reactive oxygen species incrementally reduced to light, magnetics fields, combination, wherein statistical significance was only achieved modality. By contrast, ATP production most greatly combination with indicating that mitochondrial respiratory efficiency improved plus light. Protein expression pertaining preferentially modality, as protein levels TRPC1 cation channel had been previously implicated part a calcium–mitochondrial signaling axis invoked necessary proliferation. These results indicate facilitates functional ultimately impinge on mitochondria-dependent developmental Aminoglycoside antibiotics (AGAs) have shown inhibit TRPC1-mediated magnetotransduction, whereas influence over photomodulation has not explored. Streptomycin applied during or respective enhancements, streptomycin added after did not. Magnetic modality capable partially overcoming antagonism treatment, alone The photon-electromagnetic implicates calcium entry both magnetotransduction photomodulation. Avoiding prophylactic use AGAs therapy will be crucial maintaining clinical efficacy is common concern other regenerative paradigms.

Language: Английский

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Are Aminoglycoside Antibiotics TRPing Your Metabolic Switches?

Cells, Journal Year: 2024, Volume and Issue: 13(15), P. 1273 - 1273

Published: July 29, 2024

Transient receptor potential (TRP) channels are broadly implicated in the developmental programs of most tissues. Amongst these tissues, skeletal muscle and adipose noteworthy for being essential establishing systemic metabolic balance. TRP respond to environmental stimuli by supplying intracellular calcium that instigates enzymatic cascades consequence often impinge on mitochondrial function biogenesis. Critically, aminoglycoside antibiotics (AGAs) have been shown block capacity conduct entry into cell response a wide range biophysical nature, including mechanical, electromagnetic, thermal, chemical. Paradoxically, vitro paradigms commonly used understand organismal development may led astray conventional use streptomycin, an AGA, help prevent bacterial contamination. Accordingly, streptomycin has disrupt both vivo myogenesis, as well phenotypic switch white beige thermogenic status. In vivo, TRP-mediated calcium-dependent exercise adaptations importance metabolism. Alternatively, also curb detrimental levels leakage dystrophic through aberrantly gated TRPC1 be involved etiology X-linked muscular dystrophies. susceptible AGA antagonism critically modulating tissues that, if administered behaving animals, translate systemwide disruption. Regenerative medicine clinical communities need made aware this caveat usage seek viable alternatives, contamination or infection paradigms, respectively.

Language: Английский

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Electromagnetic field‐induced adaptive response in Schwann cells through DNA methylation, histone deacetylation, and oxidative stress

Journal of Cellular Physiology, Journal Year: 2024, Volume and Issue: 239(11)

Published: June 30, 2024

Schwannomas are benign tumors of the peripheral nervous system arising from transformation Schwann cells (SCs). On whole, these related to alterations neurofibromin type 2 gene, coding for oncosuppressor merlin, a cytoskeleton-associated protein belonging ezrin-radixin-moesin family. However, underlying mechanisms schwannoma onset and progression not fully elucidated, whereas one challenges might be environment. In this light, exposure electromagnetic field (EMF), generated by use common electrical devices, has been defiantly suggested as cause SCs even if evidence was mostly epidemiologic. Indeed, insubstantial have so far identified explain oncotransformation. Recently, some in vitro pointed out proliferation migration abilities exposed EMF (0.1 T, 50 Hz, 10 min). Here, we used same experimental paradigma discuss involvement putative epigenetic adaptation occurrence hypoxic after exposure. Our findings indicate set environmental-induced changes SCs, toward less-physiological state, which may pathologically relevant differentiation development.

Language: Английский

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Exploring Mitochondrial Interactions with Pulsed Electromagnetic Fields: An Insightful Inquiry into Strategies for Addressing Neuroinflammation and Oxidative Stress in Diabetic Neuropathy

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 7783 - 7783

Published: July 16, 2024

Pulsed electromagnetic fields (PEMFs) are recognized for their potential in regenerative medicine, offering a non-invasive avenue tissue rejuvenation. While prior research has mainly focused on effects bone and dermo-epidermal tissues, the impact of PEMFs nervous tissue, particularly context neuropathy associated with diabetic foot, remains relatively unexplored. Addressing this gap, our preliminary vitro study investigates complex magnetic (CMFs) glial-like cells derived from mesenchymal cell differentiation, serving as model foot. Through assessments cellular proliferation, hemocompatibility, mutagenicity, mitochondrial membrane potential, we have established safety profile system. Furthermore, analysis microRNAs (miRNAs) suggests that CMFs may exert beneficial cycle regulation, evidenced by upregulation miRNAs within 121, 127, 142 families, which known to be function control. This exploration holds promise applications mitigating neuropathic complications foot conditions.

Language: Английский

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Frequency-Dependent Antioxidant Responses in HT-1080 Human Fibrosarcoma Cells Exposed to Weak Radio Frequency Fields

Antioxidants, Journal Year: 2024, Volume and Issue: 13(10), P. 1237 - 1237

Published: Oct. 15, 2024

This study explores the complex relationship between radio frequency (RF) exposure and cancer cells, focusing on HT-1080 human fibrosarcoma cell line. We investigated modulation of reactive oxygen species (ROS) key antioxidant enzymes, including superoxide dismutase (SOD), peroxidase, glutathione (GSH), as well mitochondrial levels viability. Exposure to RF fields in 2-5 MHz range at very weak intensities (20 nT) over 4 days resulted distinct, frequency-specific cellular effects. Significant increases SOD GSH were observed 4.5 MHz, accompanied by reduced enhanced viability, suggesting improved function. In contrast, lower frequencies like 2.5 induced oxidative stress, evidenced depletion increased levels. The findings demonstrate that cells exhibit sensitivity even significantly below current safety standards, highlighting need reassess limits. Additionally, our analysis radical pair mechanism (RPM) offers deeper insight into RF-induced responses. ROS enzyme activities is significant for treatment has broader implications age-related diseases, where stress a central factor degeneration. propose may serve therapeutic tool selectively modulate function with antioxidants playing role mitigating potential adverse

Language: Английский

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