Dumbbell probe-bridged CRISPR/Cas13a and nicking-mediated DNA cascade reaction for highly sensitive detection of colorectal cancer-related microRNAs

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 273, P. 117190 - 117190

Published: Jan. 21, 2025

Language: Английский

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MicroRNAs in disease States

Clinica Chimica Acta, Journal Year: 2025, Volume and Issue: 569, P. 120187 - 120187

Published: Feb. 10, 2025

Language: Английский

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MicroRNAs as Potential Biomarkers in Gynecological Cancers

Biomedicines, Journal Year: 2023, Volume and Issue: 11(6), P. 1704 - 1704

Published: June 13, 2023

MicroRNAs are non-coding transcripts that, thanks to the ability regulate mRNA of target genes, can affect expression genes encoding tumor suppressors and oncogenes. They control many important cellular processes, including apoptosis, differentiation, growth, division, metabolism. Therefore, miRNAs play an role in development cancers, gynecological cancers. Ovarian cancer, endometrial cervical vulvar cancer most common cancers women a frequent cause death. The heterogeneity pathogenesis these diseases makes diagnostic process significant obstacle for modern medicine. To date, studies have been carried out, which particular attention has paid molecular pathomechanism diseases, with emphasis on miRNAs. changed profile miRNAs, influenced promotion proliferation, migration, invasion processes simultaneous inhibition programmed cell death, proven times. Detailed understanding effects above-mentioned will enable potential predictive prognostic biomarkers, as well optimization process.

Language: Английский

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Angiogenesis—An Emerging Role in Organ Fibrosis

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(18), P. 14123 - 14123

Published: Sept. 15, 2023

In recent years, the study of lymphangiogenesis and fibrotic diseases has made considerable achievements, accumulating evidence indicates that plays a key role in process fibrosis various organs. Although effects on disease have not been conclusively determined due to different models pathological stages organ fibrosis, its importance development is unquestionable. Therefore, we expounded characteristics from source lymphatic endothelial cells (LECs), mechanism fibrosis-related lymphangiogenesis, therapeutic effect intervening fibrosis. We found expansion LECs or networks occurs through original cell budding macrophage differentiation into LECs, vascular growth factor C (VEGFC)/vascular receptor (VEGFR3) pathway central lymphangiogenesis. Lymphatic vessel hyaluronan 1 (LYVE1), as also involved regulation Intervention with improves some extent. complex microenvironment, variety functional cells, inflammatory factors chemokines synergistically antagonistically form network regulate progression disease. Further clarifying formation new may potentially provide strategies for treatment

Language: Английский

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Mechanism underlying the role of the circRNA OMA1/miR-654-3p/RAF1 axis in children with inflammatory bowel disease

Cytotechnology, Journal Year: 2025, Volume and Issue: 77(2)

Published: Jan. 25, 2025

Language: Английский

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Basic Principles of RNA Interference: Nucleic Acid Types and In Vitro Intracellular Delivery Methods

Micromachines, Journal Year: 2023, Volume and Issue: 14(7), P. 1321 - 1321

Published: June 27, 2023

Since its discovery in 1989, RNA interference (RNAi) has become a widely used tool for the vitro downregulation of specific gene expression molecular biological research. This basically involves complementary that binds target sequence to affect transcription or translation process. Currently, various small RNAs, such as interfering (siRNA), micro (miRNA), hairpin (shRNA), and PIWI interacting (piRNA), are available application on cell culture, regulate cells' by mimicking endogenous RNAi-machinery. In addition, several biochemical, physical, viral methods have been established deliver these RNAs into nucleus. each delivery method entail different off-target effects, limitations, compatibilities, it is crucial understand their basic mode action. review intended provide an overview nucleic acids planning, interpreting, troubleshooting RNAi experiments.

Language: Английский

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Novel insight into miRNA biology and its role in the pathogenesis of systemic lupus erythematosus

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: Dec. 2, 2022

MicroRNAs(miRNAs) have emerged as key regulators that control and influence gene expression well multiple biological processes depending on their potential binding sites in human-protein coding genes other unconventional patterns, including for peptides, activating Toll-like receptors a ligand, manners. Accumulating evidence has demonstrated microRNA is tightly regulated during phases of development, differentiation, effector functions immune cells, immunological disorders systemic lupus erythematosus (SLE). This review outlines the biogenesis miRNAs underlying cellular molecular mechanisms. It then summarizes our current knowledge about how regulated. Moreover, an overview was provided concerning role abnormal cells. In particular, we will shed some light recent advances exosome-derived epigenetic pathways pathogenesis SLE.

Language: Английский

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Analysing the relevance of TGF-β and its regulators in cervical cancer to identify therapeutic and diagnostic markers

Gene, Journal Year: 2024, Volume and Issue: 938, P. 149166 - 149166

Published: Dec. 18, 2024

Language: Английский

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DAE-CFR: detecting microRNA-disease associations using deep autoencoder and combined feature representation

BMC Bioinformatics, Journal Year: 2024, Volume and Issue: 25(1)

Published: March 29, 2024

Abstract Background MicroRNA (miRNA) has been shown to play a key role in the occurrence and progression of diseases, making uncovering miRNA-disease associations vital for disease prevention therapy. However, traditional laboratory methods detecting these are slow, strenuous, expensive, uncertain. Although numerous advanced algorithms have emerged, it is still challenge develop more effective explore underlying associations. Results In study, we designed novel approach on basis deep autoencoder combined feature representation (DAE-CFR) predict possible We began by creating integrated similarity matrices miRNAs performing logistic function transformation, balancing positive negative samples with k -means clustering, constructing training samples. Then, was used extract low-dimensional from two kinds representations namely, original association information-based information-based. Next, resulting features each pair regression (LR) classifier infer all unknown interactions. Under five tenfold cross-validation (CV) frameworks, DAE-CFR not only outperformed six popular nine classifiers, but also demonstrated superior performance an additional dataset. Furthermore, case studies three diseases (myocardial infarction, hypertension stroke) confirmed validity practice. Conclusions achieved outstanding predicting can provide evidence inform biological experiments clinical

Language: Английский

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Progress in Research of miRNA-378a Regulating Immune Response and Osteogenic Angiogenesis

Advances in Clinical Medicine, Journal Year: 2024, Volume and Issue: 14(03), P. 1309 - 1316

Published: Jan. 1, 2024

Language: Английский

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