Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 278, P. 117337 - 117337
Published: March 7, 2025
Language: Английский
Diagnostics, Journal Year: 2023, Volume and Issue: 13(5), P. 824 - 824
Published: Feb. 21, 2023
Monkeypox or Mpox is an infectious virus predominantly found in Africa. It has spread to many countries since its latest outbreak. Symptoms such as headaches, chills, and fever are observed humans. Lumps rashes also appear on the skin (similar smallpox, measles, chickenpox). Many artificial intelligence (AI) models have been developed for accurate early diagnosis. In this work, we systematically reviewed recent studies that used AI mpox-related research. After a literature search, 34 fulfilling prespecified criteria were selected with following subject categories: diagnostic testing of mpox, epidemiological modeling mpox infection spread, drug vaccine discovery, media risk management. beginning, detection using various modalities was described. Other applications ML DL mitigating categorized later. The machine deep learning algorithms their performance discussed. We believe state-of-the-art review will be valuable resource researchers data scientists developing measures counter spread.
Language: Английский
Citations
55
Journal of Medical Virology, Journal Year: 2022, Volume and Issue: 95(1)
Published: Dec. 8, 2022
The global outbreak of the monkeypox virus (MPXV) highlights need for rapid and cost-effective MPXV detection tools to effectively monitor control disease. Herein, we demonstrated a portable CRISPR-Cas-based system naked-eye MPXV. harnesses high selectivity CRISPR-Cas12 isothermal nucleic acid amplification potential recombinase polymerase amplification. It can detect both current circulating clade original clades. We reached limit (LoD) 22.4 aM (13.5 copies/µl) using microtiter plate reader, while visual LoD is 75 (45 in two-step assay, which further reduced 25 (15 one-pot system. compared our results with quantitative chain reaction obtained satisfactory consistency. For clinical application, sensitive precise method attomolar sensitivity sample-to-answer time 35 min.
Language: Английский
Citations
53
Biosensors, Journal Year: 2022, Volume and Issue: 13(1), P. 29 - 29
Published: Dec. 26, 2022
Infectious pathogens cause severe threats to public health due their frightening infectivity and lethal capacity. Rapid accurate detection of is great significance for preventing infection. Gold nanoparticles have drawn considerable attention in colorimetric biosensing during the past decades unique physicochemical properties. Colorimetric diagnosis platforms based on functionalized AuNPs are emerging as a promising pathogen-analysis technique with merits high sensitivity, low-cost, easy operation. This review summarizes recent development this field. We first introduce detecting characteristics gold nanoparticles. Four types strategies, including application indirect target-mediated aggregation, chromogenic substrate-mediated catalytic activity, point-of-care testing (POCT) devices, machine learning-assisted sensor arrays, systematically introduced. In particular, three biomolecule-functionalized AuNP-based sensors described detail. Finally, we conclude by presenting our subjective views present challenges some appropriate suggestions future research directions sensors.
Language: Английский
Citations
53
The Analyst, Journal Year: 2023, Volume and Issue: 148(5), P. 985 - 994
Published: Jan. 1, 2023
In 2022, the outbreak of monkeypox virus occurred in many non-endemic countries, and World Health Organization (WHO) assessed that this was "atypical". The establishment a rapid effective assay can be used for early diagnosis infection is crucial prevention control. study, A29 protein homologous vaccinia A27 cowpox 162 were expressed Escherichia coli BL21 screening. We synthesized A2917-49 peptide as immunogen obtained 25 monoclonal antibodies (mAbs) against using mouse hybridoma techniques. Then an immunochromatographic test strip method detecting established. strips utilizing mAb-7C5 5D8 showed best sensitivity lowest limit detection: 50 pg mL-1 purified specificity tests did not cross-react with other orthopox viruses (vaccinia or virus) well common respiratory pathogens (SARS-CoV-2, influenza A B). Therefore, by antigen detection.
Language: Английский
Citations
38
Journal of Colloid and Interface Science, Journal Year: 2024, Volume and Issue: 660, P. 412 - 422
Published: Jan. 5, 2024
Language: Английский
Citations
14
Emerging Microbes & Infections, Journal Year: 2024, Volume and Issue: 13(1)
Published: March 22, 2024
With the large number of atypical cases in mpox outbreak, which was classified as a global health emergency by World Health Organization (WHO) on 23 July 2022, rapid diagnosis and diseases with similar symptoms to such chickenpox respiratory infectious early stages viral infection is key controlling spread outbreak. In this study, antibodies against monkeypox virus A29L protein were efficiently rapidly identified combining mRNA immunization high-throughput sequencing individual B cells. We obtained eight high affinity for validated ELISA, used basis developing an ultrasensitive fluorescent immunochromatographic assay based multilayer quantum dot nanobeads (SiTQD-ICA). The SiTQD-ICA biosensor utilizing M53 M78 showed sensitivity stability detection: detected within 20 min, minimum detection limit 5 pg/mL. A specificity test that method non-cross-reactive or common pathogens can be antigen detection. This antibody identification also acquisition monoclonal outbreaks other various studies.
Language: Английский
Citations
10
Journal of Pharmaceutical Analysis, Journal Year: 2024, Volume and Issue: 14(10), P. 100995 - 100995
Published: May 7, 2024
The efficient immobilization of capture antibodies is crucial for timely pathogen detection during global pandemic outbreaks. Therefore, we proposed a silica-binding protein featuring core functional domains (cSP). It comprises peptide with tag designed to adhere silica surfaces and tandem G fragments (2C2) effective antibody capture. This innovation facilitates precise site-directed onto surfaces. We applied cSP silica-coated optical fibers, creating fiber-optic biolayer interferometer (FO-BLI) biosensor capable monitoring the monkeypox virus (MPXV) A29L in spiked clinical samples rapidly detect MPXV. cSP-based FO-BLI MPXV demonstrated limit (LOD) 0.62 ng/mL buffer, comparable 0.52 LOD achieved using conventional streptavidin (SA)-based biosensor. Furthermore, it LODs 0.77 serum 0.80 saliva, exhibiting no cross-reactivity other viral antigens. process was completed within 14 min. further multi-virus strategy detecting various strains, such as MPXV, latest coronavirus disease (COVID) variants, influenza A protein, extend its versatility. cSP-modified has high potential accurately antigens, making valuable contributions epidemiological studies.
Language: Английский
Citations
10
Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 278, P. 117221 - 117221
Published: Jan. 30, 2025
Language: Английский
Citations
2
TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 167, P. 117226 - 117226
Published: Aug. 9, 2023
Language: Английский
Citations
18
Microchimica Acta, Journal Year: 2024, Volume and Issue: 191(2)
Published: Jan. 17, 2024
Language: Английский
Citations
9