PhotoniX,
Journal Year:
2024,
Volume and Issue:
5(1)
Published: Oct. 11, 2024
Abstract
Photonic
integrated
circuits
(PICs)
represent
a
promising
technology
for
the
much-needed
medical
devices
of
today.
Their
primary
advantage
lies
in
their
ability
to
integrate
multiple
functions
onto
single
chip,
thereby
reducing
complexity,
size,
maintenance
requirements,
and
costs.
When
applied
optical
coherence
tomography
(OCT),
leading
tool
state-of-the-art
ophthalmic
diagnosis,
PICs
have
potential
increase
accessibility,
especially
scenarios,
where
weight,
or
costs
are
limiting
factors.
In
this
paper,
we
present
PIC-based
CMOS-compatible
spectrometer
spectral
domain
OCT
with
an
unprecedented
level
integration.
To
achieve
this,
co-integrated
512-channel
arrayed
waveguide
grating
electronics.
We
successfully
addressed
challenge
establishing
connection
from
waveguides
photodiodes
monolithically
on
chip
minimal
losses
achieving
coupling
efficiency
70%.
With
fully
interfaced
system,
reached
sensitivity
92dB
at
imaging
speed
55kHz,
6dB
signal
roll-off
occurring
2mm.
innovative
obtain
3D
vivo
tomograms
zebrafish
larvae
human
skin.
This
ground-breaking
represents
significant
step
towards
miniaturised,
cost-effective,
maintenance-free
system.
Cancers,
Journal Year:
2022,
Volume and Issue:
15(1), P. 139 - 139
Published: Dec. 26, 2022
Cancer
is
a
heterogeneous
disease.
Each
individual
tumor
unique
and
characterized
by
structural,
cellular,
genetic
molecular
features.
Therefore,
patient-derived
cancer
models
are
indispensable
tools
in
research
have
been
actively
introduced
into
the
healthcare
system.
For
instance,
provide
good
reproducibility
of
susceptibility
resistance
cells
against
drugs,
allowing
personalized
therapy
for
patients.
In
this
article,
we
review
advantages
disadvantages
following
cancer:
(1)
PDC—patient-derived
cell
culture,
(2)
PDS—patient-derived
spheroids
PDO—patient-derived
organoids,
(3)
PDTSC—patient-derived
tissue
slice
cultures,
(4)
PDX—patient-derived
xenografts,
humanized
PDX,
as
well
PDXC—PDX-derived
cultures
PDXO—PDX-derived
organoids.
We
also
an
overview
current
clinical
investigations
new
developments
area
models.
Moreover,
attention
paid
to
databases
models,
which
collected
specialized
repositories.
believe
that
widespread
use
will
improve
our
knowledge
biology
contribute
development
more
effective
treatment
strategies.
Clinical & Translational Oncology,
Journal Year:
2023,
Volume and Issue:
26(2), P. 311 - 325
Published: July 3, 2023
Abstract
Glioblastoma
(GBM)
constitutes
the
most
common
primary
brain
tumor
in
adults.
The
challenges
GBM
therapeutics
have
shed
light
on
zebrafish
used
as
a
promising
animal
model
for
preclinical
xenograft
studies
without
standardized
methodology.
This
systematic
review
aims
to
summarize
advances
xenografting,
compare
research
protocols
pinpoint
advantages
and
underlying
limitations,
designate
predominant
xenografting
parameters.
Based
PRISMA
checklist,
we
systematically
searched
PubMed,
Scopus,
ZFIN
using
keywords
“glioblastoma,”
“xenotransplantation,”
“zebrafish”
papers
published
from
2005
2022,
available
English.
46
articles
meeting
criteria
were
examined
strain,
cancer
cell
line,
labeling
technique,
injected
number,
time
site
of
injection,
maintenance
temperature.
Our
designated
that
AB
wild-type
zebrafish,
Casper
transparent
mutants,
transgenic
Tg(fli1:EGFP),
or
crossbreeding
these
predominate
among
strains.
Orthotopic
transplantation
is
more
commonly
employed.
A
number
50–100
cells
at
48
h
post-fertilization
high
density
low
infusion
volume
considered
an
effective
approach.
U87
are
angiogenesis
studies,
U251
proliferation
patient-derived
(PDX)
achieve
clinical
relevance.
Gradual
acclimatization
32–33
°C
can
partly
address
temperature
differential
between
cells.
Zebrafish
models
constitute
valuable
tools
with
relevance
regarding
PDX.
requires
modification
based
objective
each
team.
Automation
further
optimization
protocol
parameters
could
scale
up
anticancer
drug
trials.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(3), P. 2288 - 2288
Published: Jan. 24, 2023
The
increasing
number
of
available
anti-cancer
drugs
presents
a
challenge
for
oncologists,
who
must
choose
the
most
effective
treatment
patient.
Precision
cancer
medicine
relies
on
matching
drug
with
tumor's
molecular
profile
to
optimize
therapeutic
benefit.
However,
current
precision
approaches
do
not
fully
account
intra-tumoral
heterogeneity.
Different
mutation
profiles
and
cell
behaviors
within
single
heterogeneous
tumor
can
significantly
impact
therapy
response
patient
outcomes.
Patient-derived
avatar
models
recapitulate
patient's
in
an
animal
or
dish
provide
means
functionally
assess
heterogeneity's
response.
Mouse
xenograft
organoid
avatars
are
well-established,
but
time
required
generate
these
is
practical
clinical
decision-making.
Zebrafish
emerging
as
time-efficient
cost-effective
model.
In
this
review,
we
highlight
recent
developments
zebrafish
discuss
unique
features
that
make
them
ideal
interrogation
heterogeneity
part
pipelines.
Cell Death and Disease,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Jan. 9, 2024
Abstract
Colorectal
cancer
(CRC)
is
the
second
leading
cause
of
cancer-related
death,
mostly
due
to
metastatic
disease
and
fact
that
many
patients
already
show
signs
metastasis
at
time
first
diagnosis.
Current
CRC
therapies
negatively
impact
patients’
quality
life
have
little
no
effect
on
combating
tumor
once
dissemination
has
started.
Danio
rerio
(zebrafish)
a
popular
animal
model
utilized
in
research.
One
its
main
advantages
ease
xenograft
transplantation
zebrafish
larvae
lack
adaptative
immune
system,
guaranteeing
impossibility
rejection.
In
this
review,
we
presented
works
choose
as
tool
for
study
CRC,
highlighting
methods
used
well
promising
new
therapeutic
molecules
been
identified
model.
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: June 5, 2024
Abstract
Cancer
patients
often
undergo
rounds
of
trial-and-error
to
find
the
most
effective
treatment
because
there
is
no
test
in
clinical
practice
for
predicting
therapy
response.
Here,
we
conduct
a
study
validate
zebrafish
patient-derived
xenograft
model
(zAvatar)
as
fast
predictive
platform
personalized
colorectal
cancer.
zAvatars
are
generated
with
patient
tumor
cells,
treated
exactly
same
their
corresponding
and
analyzed
at
single-cell
resolution.
By
individually
comparing
responses
55
zAvatar-test,
develop
decision
tree
integrating
stage,
zAvatar-apoptosis,
zAvatar-metastatic
potential.
This
accurately
forecasts
progression
91%
accuracy.
Importantly,
sensitive
zAvatar-test
exhibit
longer
progression-free
survival
compared
those
resistant
test.
We
propose
rapid
approach
guide
decisions,
optimizing
options
improving
cancer
patients.
Antioxidants,
Journal Year:
2021,
Volume and Issue:
10(5), P. 668 - 668
Published: April 25, 2021
The
zebrafish
is
considered
one
of
the
most
versatile
experimental
animal
models.
transparency
embryos,
small
size,
rapid
development
and
homology
with
higher
vertebrates
have
made
a
valuable
model
also
for
drug
screening.
Its
use
closely
related
determination
bioactivity,
toxicity
off-target
side
effects
novel
candidates,
which
allows
thorough
evaluation
new
targets;
thus,
it
may
represent
suitable
screening
optimization
candidates.
Flavonoids
are
polyphenolic
compounds
widely
present
in
fruits,
vegetables
cereals.
Polyphenols
important
both
plants
humans,
considering
their
involvement
defense
mechanisms,
particularly
against
oxidative
stress.
They
protect
from
biotic
abiotic
stressors
prevent
or
treat
oxidative-based
human
diseases.
For
these
reasons,
polyphenols
used
as
nutraceuticals,
functional
foods
supplements
by
pharmaceutical
industry.
Therefore,
relevant
findings
on
useful
to
study
stress-linked
disorders,
focusing
biological
activities
flavonoids,
here
summarized
reviewed.
Marine Drugs,
Journal Year:
2023,
Volume and Issue:
21(12), P. 607 - 607
Published: Nov. 24, 2023
The
discovery
of
new
highly
effective
anticancer
drugs
with
few
side
effects
is
a
challenge
for
drug
development
research.
Natural
or
synthetic
peptides
(ACPs)
represent
generation
agents
high
selectivity
and
specificity.
rapid
emergence
chemoradiation-resistant
lung
cancer
has
necessitated
the
novel
as
alternatives
to
conventional
therapeutics.
In
this
study,
we
synthesized
peptide
containing
22
amino
acids
characterized
it
ACP
(MP06)
derived
from
green
sea
algae,
Bryopsis
plumosa.
Using
database,
MP06
was
predicted
possess
an
alpha-helical
secondary
structure
functionality.
anti-proliferative
apoptotic
MP06,
determined
using
cytotoxicity
assay
Annexin
V/propidium
iodide
staining
kit,
were
significantly
higher
in
non-small-cell
(NSCLC)
cells
than
non-cancerous
cells.
We
confirmed
that
suppressed
cellular
migration
invasion
inhibited
expression
N-cadherin
vimentin,
markers
epithelial-mesenchymal
transition.
Moreover,
effectively
reduced
metastasis
tumor
xenografts
zebrafish
embryos.
conclusion,
suggest
considering
candidate
functioning
via
ERK
signaling
pathway.
