Molecules,
Journal Year:
2023,
Volume and Issue:
28(18), P. 6654 - 6654
Published: Sept. 16, 2023
Sodium
chloride
(NaCl)
is
a
commonly
used
additive
in
minimally
processed
fish-based
products.
The
addition
of
NaCl
to
fish
products
and
packaging
modified
atmosphere
usually
efficient
with
regard
limiting
the
occurrence
aquatic
environmental
pathogen
Pseudomonas
aeruginosa.
Given
negative
effects
excess
diet,
there
growing
demand
reduce
food
safer
substituents,
but
knowledge
their
impact
on
antibiotic
resistant
P.
aeruginosa
limited.
This
study
aimed
evaluate
physiological
transcriptome
characteristics
NT06
isolated
from
determine
effect
selected
concentrations
alternative
compounds
(KCl/NaL/NaC)
virulence
phenotype
genotype.
In
study,
among
microorganisms,
showed
highest
resistance
(to
ampicillin,
ceftriaxone,
nalidixic
acid,
norfloxacin)
ability
grow
at
4
°C.
Comprehensive
Antibiotic
Resistance
Database
(CARD)
Virulence
Factor
(VFDB)
revealed
presence
24
134
gene
assigned
AMR
VF
transcriptome,
respectively.
KCl,
KCl/NaL
KCl/NaL/NaC
inhibited
pyocyanin
biosynthesis,
elastase
activity,
protease
activity
40
77%.
above
phenotypic
observations
were
confirmed
via
RT-qPCR
analyses,
which
that
all
tested
genes
most
downregulated
due
treatment.
conclusion,
this
provides
insight
into
potential
foodborne
possible
impairment
those
features
by
NaL,
NaC,
exert
synergistic
can
be
AMB Express,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Aug. 1, 2024
Abstract
Resistance
to
antibiotics
is
a
critical
growing
public
health
problem
that
desires
urgent
action
combat.
To
avoid
the
stress
on
bacterial
growth
evokes
resistance
development,
anti-virulence
agents
can
be
an
attractive
strategy
as
they
do
not
target
growth.
Quorum
sensing
(QS)
systems
play
main
roles
in
controlling
production
of
diverse
virulence
factors
and
biofilm
formation
bacteria.
Thus,
interfering
with
QS
could
result
mitigation
virulence.
Cilostazol
antiplatelet
vasodilator
FDA
approved
drug.
This
study
aimed
evaluate
activities
cilostazol
light
its
possible
interference
Pseudomonas
aeruginosa
.
Additionally,
examines
cilostazol’s
impact
bacterium’s
ability
induce
infection
vivo,
using
sub-inhibitory
concentrations
minimize
risk
development.
In
this
context,
formation,
influence
vivo
were
assessed
presence
at
concentration.
Furthermore,
outcome
combination
was
evaluated.
interfered
P.
Moreover,
swarming
motility,
significantly
diminished.
Histopathological
investigation
revealed
liver,
spleen
kidney
tissues
damage
abolished
mice
injected
cilostazol-treated
exhibited
synergistic
when
used
antibiotics.
At
molecular
level,
downregulated
genes
showed
considerable
affinity
receptors.
conclusion,
adjunct
therapy
for
treating
Pseudomonal
infections.
research
highlights
potential
combat
infections
by
targeting
mechanisms,
reducing
antibiotic
resistance,
enhancing
treatment
efficacy
against
These
findings
open
avenues
repurposing
existing
drugs,
offering
new,
safer,
more
effective
control
strategies.
ACS Omega,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 16, 2025
Sulforaphane
is
a
main
bioactive
component
in
several
edible
cruciferous
vegetables.
It
acquires
benefits
to
health
addition
its
considered
antibacterial
and
antivirulence
activities.
Herein,
we
aimed
at
evaluating
the
activity
of
sulforaphane
against
worldwide
clinically
important
enteric
pathogen
Salmonella
enterica
serovar
Typhimurium.
The
influence
on
bacterial
adhesion,
invasion,
biofilm
formation,
intracellular
replication
was
assayed.
Additionally,
effect
type
III
secretion
system
(TTSS)
S.
quantified.
outcome
combination
with
different
antibiotics
assessed,
an
vivo
protection
assay
conducted
assess
pathogenesis.
results
showed
significant
antibiofilm
subinhibitory
reduction
invasion
inside
host
cells.
findings
emphasized
decreased
capacity
induce
pathogenesis
presence
sulforaphane.
Our
finding
attributed
these
activities
interference
TTSS-type
II
downregulation
encoding
genes.
In
nutshell,
vegetable
safe
adjunct
therapy
that
can
be
administrated
alongside
traditional
for
treating
pathogens
as
enterica.
AMB Express,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Jan. 29, 2025
Candida
albicans
is
a
commensal
fungus
that
naturally
inhabits
the
vagina.
However,
overgrowth
of
C.
can
result
in
vulvovaginal
candidiasis
(VVC),
one
most
prevalent
fungal
infections
affecting
women.
The
rapid
emergence
azole
resistance
albicans,
addition
to
limited
available
antifungal
agents,
complicates
treatment
and
emphasizes
urgent
need
for
novel
therapeutic
options.
Efflux-mediated
common
mechanism
fluconazole
(FLZ)-resistant
albicans.
Combination
therapy
using
natural
compounds
potential
approach
restore
fluconazole's
activity
azole-resistant
isolates
via
efflux
pump
inhibition.
This
study
aimed
evaluate
ability
celastrol,
triterpene,
retrieve
FLZ
against
vitro
vivo.
Celastrol
did
not
exhibit
tested
clinical
isolates;
however,
sub-MIC
celastrol
inhibited
rhodamine
6G
(R6G)
increased
R6G
accumulation
inside
celastrol-treated
cells.
Synergy
was
spotted
between
checkerboard
assay.
Quantification
m-RNA
levels
efflux-mediated
genes
within
demonstrated
CDR1
overexpression.
Upon
treatment,
significant
decline
ABC
transporters
transcript
were
detected.
Moreover,
molecular
docking
blocker
successfully
fits
into
target
binding
pockets.
A
negligible
hemolytic
effect
human
erythrocytes
observed.
In
vivo
model
VVC,
combination
vaginal
gel
revealed
drastic
reduction
burden
with
apparently
normal
tissue.
promising
findings
strengthen
its
future
use
Eurosurveillance,
Journal Year:
2025,
Volume and Issue:
30(5)
Published: Feb. 6, 2025
We
report
a
national
outbreak
of
Serratia
marcescens
complex
type
755
(ct755)
in
Norway,
with
74
cases
identified
between
June
2021
and
February
2023.
Careful
reviews
patient
journals
interviews
were
performed,
involving
33
hospitals
throughout
Norway.
All
available
clinical
isolates
S.
collected
January
2023
(n
=
455,
including
cases)
from
all
involved
whole
genome
sequenced.
Cases
displayed
pattern
opportunistic
infections,
as
usually
observed
marcescens.
No
epidemiological
links,
common
exposures
or
risk
factors
identified.
The
investigation
pointed
to
an
source
present
the
community.
suspect
nationally
distributed
product,
possibly
food
source.
Phylogenetic
analysis
revealed
highly
diverse
bacterial
population
containing
multiple
distinct
clusters.
cluster
ct755
stands
out
largest
least
clone
continuum,
however
second
(ct281)
also
triggered
separate
investigation.
This
highlights
challenges
outbreaks
caused
by
pathogens
suggests
that
presence
identical
strains
samples
is
more
than
previously
recognised.
AMB Express,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Feb. 22, 2025
Resistance
to
antibiotics
is
a
critical
growing
public
health
problem
that
needs
urgent
action
combat.
To
avoid
the
stress
on
bacterial
growth
evokes
development
of
resistance,
anti-virulence
agents
can
be
an
attractive
strategy
as
they
do
not
target
growth.
There
are
FDA
approved
drugs
have
been
screened
for
their
activities.
Lamivudine
(LAM)
synthetic
nucleoside
analogue
used
antiretroviral
in
treatment
HIV
and
HBV.
The
present
study
aimed
assess
activities
LAM
against
clinically
important
pathogen
Pseudomonas
aeruginosa.
LAM's
antibiofilm
were
evaluated.
impact
quorum
sensing
(QS)
systems
which
control
production
these
virulence
factors
was
assessed
virtually
by
quantification
expression
QS-encoding
genes.
Furthermore,
vivo
mice
protection
assay
conducted
attest
anti-pathogenic
activity.
current
findings
elaborated
promising
anti-QS
LAM.
interfered
with
biofilm
formation
P.
aeruginosa
PAO1
strain.
Moreover,
swarming
motility
pyocyanin
protease
significantly
diminished.
At
molecular
level,
downregulated
genes
LasI,
LasR,
RhlR,
PqsA
PqsR.
Additionally,
detailed
silico
docking
simulation
studies
showed
considered
high
ability
bind
hinder
QS
receptors
In
agreement
vitro
silico,
results
full
conclusion,
could
repurposed
employed
adjunct
therapy
traditional
treating
serious
pseudomonal
infections.
