Transcriptomic and metabolomic analysis reveals the molecular mechanism of exogenous melatonin improves salt tolerance in eggplants

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 10, 2025

Melatonin significantly enhances the tolerance of plants to biotic and abiotic stress, plays an important role in plant resistance salt stress. However, its molecular mechanisms eggplant stress have been rarely reported. In previous studies, we experimentally demonstrated that melatonin can enhance eggplants. this study, treated salt-stressed with a control treatment water, then conducted physiological biochemical tests, transcriptomic metabolomic sequencing, RT-qPCR validation at different stages after treatment. The results showed exogenous alleviate adverse effects on by increasing activity antioxidant enzymes, reducing content reactive oxygen species plants, organic osmoprotectants. Transcriptomic data, as well combined analysis, indicate activate metabolic pathways Compared genes α-linolenic acid metabolism pathway promote accumulation metabolites pathway, significant observed 48 hours treatment, activates expression such SmePLA2, SmeLOXs SmeOPR et al. α-Linolenic acid, (9R,13R)-12-oxophytodienoic 9(S)-HpOTrE (+)-7-iso-Jasmonic acid. validated activating effect candidate a-linolenic pathway. This study analyzed mechanism alleviating providing theoretical foundation for application enhancing production.

Language: Английский

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Combining Physiology and Transcriptome to Reveal Mechanisms of Hosta ‘Golden Cadet’ in Response to Alkali Stress

Plants, Journal Year: 2025, Volume and Issue: 14(4), P. 593 - 593

Published: Feb. 15, 2025

As an ornamentally and medicinally worthy plant, Hosta plantaginea (Lam.) Aschers. has the adapted capacity to survive cold temperate monsoon climates in Northeastern China. However, its use is limited by soil alkalization of urban gardens. Our pre-experiment found that ‘Golden Cadet’ potential be alkali-tolerant. Hence, tissue-cultured seedlings were used as experimental material. Its related growth, physiology, transcripts examined reveal molecular mechanism response alkali stress. The results show development was affected In comparison with control, malondialdehyde (MDA) content increased 4.28-fold at 24th hour, superoxide dismutase (SOD) activity 49% 6th peroxidase (POD) soluble sugar (SS) 67% 30% 12th respectively. RNA-seq analysis revealed gene expressions 0 h, 6 12 21 h 48 differed after 200 mmol/L NaHCO3 treatment. During under stress, 2366 differentially expressed genes found. transcription factors MYB, AP2/ERF, WRKY activated genes. KEGG phytohormone signaling pathways, starch sucrose metabolism, phenylpropane production summary, can reduce membrane damage improving osmoregulation antioxidant capacity, increase regulate biosynthesis activating inducing signaling, mitigating effects toxicity. These findings guide investigation into tolerance plants, screening genes, selecting breeding novel alkali-tolerant cultivars.

Language: Английский

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Molecular Insights Into Salt Stress Adaptation in Plants

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

ABSTRACT The significant rise in soil salinity has had detrimental effects on global agricultural production, negatively impacting overall plant health and leading to a decline productivity. As protective response, plants have developed diverse regulatory mechanisms counteract these adverse conditions. help mitigate damage caused by both osmotic ionic stress resulting from high salinity. Given the severe threat this poses food security well‐being of world's population, scientists dedicated decades research understanding how manage salt stress. Numerous been identified studied enhance tolerance alleviate This review examines recent advancements molecular underlying salt, including uptake transport, sensing signalling, hormonal regulation, epigenetic modifications, genetic adaptation, posttranslational modifications. Although current knowledge advanced our understanding, critical gaps controversies remain, such as stability memory, trade‐off between growth, crosstalk, novel genes with uncharacterised roles tolerance. To resolve questions, further employing techniques like GWAS, transcriptomics, transgenic genome‐editing technologies, well studies energy allocation is essential. A deeper exploration complex, synergistic will pave way for enhancing resilience ensuring adaptation increasingly challenging environmental

Language: Английский

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New insights into the salt-responsive regulation in eelgrass at transcriptional and post-transcriptional levels

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 6, 2025

The adaptation mechanisms of marine plants to the environments have garnered significant attention in recent years. Eelgrass (Zostera marina), a representative angiosperm, serves as an ideal model for investigating underlying salt tolerance. This study integrated mRNA, sRNA, and degradome sequencing data identify key genes associated with tolerance eelgrass. results indicate that series involved biological processes such "in response water deprivation" "biosynthesis secondary metabolites" respond stress. Analysis cis-regulatory elements expression similarities suggests ABA synthase 9-cis-epoxycarotenoid dioxygenase (NCED) may be regulated by ERF members, while phenylalanine ammonia-lyase (PAL) MYB members. At post-transcriptional regulation level, miRNA156 miRNA166 might regulating potential target genes, members WRKY HD-ZIP families. Additionally, eelgrass exhibits unique responses salt, up-regulation "fucose biosynthetic process". These findings enhance our understanding how adapts environment. As monocotyledon, is helpful find conserved cross-species comparison. By examining transcriptional homologous eelgrass, rice, maize, we identified several groups are their gene resources provide targets genetic engineering improve crops.

Language: Английский

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Overexpression of SmGRAS5 enhances tolerance to abiotic stresses in Salvia miltiorrhiza

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109669 - 109669

Published: Feb. 1, 2025

Language: Английский

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A comprehensive review on rice responses and tolerance to salt stress

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 31, 2025

The challenge of salinity stress significantly impacts global rice production, especially in coastal and arid regions where the salinization agricultural soils is on rise. This review explores complex physiological, biochemical, genetic mechanisms contributing to tolerance (Oryza sativa L.) while examining agronomic multidisciplinary strategies bolster resilience. Essential adaptations encompass regulation ionic balance, management antioxidants, adjustments osmotic pressure, all driven by genes such as OsHKT1;5 transcription factors like OsbZIP73. evolution breeding strategies, encompassing traditional methods cutting-edge innovations, has produced remarkable salt-tolerant varieties FL478 BRRI dhan47. advancements this field are enhanced including integrated soil management, crop rotation, chemical treatments spermidine, which through antioxidant activity transcriptional mechanisms. Case studies from South Asia, Sub-Saharan Africa, Middle East and, Australia demonstrate transformative potential utilizing varieties; however, challenges persist, polygenic nature tolerance, environmental variability, socioeconomic barriers. highlights importance collaborative efforts across various disciplines, merging genomic technologies, sophisticated phenotyping, inclusive practices foster climate-resilient sustainable cultivation. work seeks navigate complexities its implications for food security, employing inventive cohesive confront posed climate change.

Language: Английский

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Plant Responses and Adaptations to Salt Stress: A Review

Horticulturae, Journal Year: 2024, Volume and Issue: 10(11), P. 1221 - 1221

Published: Nov. 18, 2024

Salinity poses a significant environmental challenge, limiting plant growth and development. To cultivate salt-tolerant plants, it is crucial to understand the physiological, biochemical, molecular responses adaptations salt stress, as well explore natural genetic resources linked tolerance. In this review, we provide detailed overview of mechanisms behind morphological physiological triggered by including damage disturbance cell osmotic potentials ion homeostasis, lipid peroxidation, suppression photosynthesis growth. We also describe that confer tolerance in such adjustments, reactive oxygen species (ROS) scavenging, photosynthetic responses, phytohormone regulation, regulation. Additionally, summarize salt-stress sensing signaling pathways, gene regulatory networks, salt-tolerance plants. The key pathways involved signal perception transduction, Ca2+-dependent protein kinase (CDPK) cascades, overly sensitive (SOS) pathway, abscisic acid (ABA) are discussed, along with relevant salt-stress-responsive genes transcription factors. end, important issues challenges related for future research addressed. Overall, review aims essential insights cultivation breeding crops fruits.

Language: Английский

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Enhancing Crop Resilience: The Role of Plant Genetics, Transcription Factors, and Next-Generation Sequencing in Addressing Salt Stress

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12537 - 12537

Published: Nov. 22, 2024

Salt stress is a major abiotic stressor that limits plant growth, development, and agricultural productivity, especially in regions with high soil salinity. With the increasing salinization of soils due to climate change, developing salt-tolerant crops has become essential for ensuring food security. This review consolidates recent advances genetics, transcription factors (TFs), next-generation sequencing (NGS) technologies are pivotal enhancing salt tolerance crops. It highlights critical genes involved ion homeostasis, osmotic adjustment, signaling pathways, which contribute resilience under saline conditions. Additionally, specific TF families, such as DREB, NAC (NAM, ATAF, CUC), WRKY, explored their roles activating salt-responsive gene networks. By leveraging NGS technologies-including genome-wide association studies (GWASs) RNA (RNA-seq)-this provides insights into complex genetic basis tolerance, identifying novel regulatory networks underpin adaptive responses. Emphasizing integration tools, research, NGS, this presents comprehensive framework accelerating development crops, contributing sustainable agriculture saline-prone areas.

Language: Английский

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