Aquaculture Reports, Journal Year: 2025, Volume and Issue: 42, P. 102738 - 102738
Published: March 13, 2025
Language: Английский
Science Advances, Journal Year: 2023, Volume and Issue: 9(9)
Published: March 1, 2023
Cellular metabolism is important for adult neural stem/progenitor cell (NSPC) behavior. However, its role in the transition from quiescence to proliferation not fully understood. We here show that mitochondrial pyruvate carrier (MPC) plays a crucial and unexpected part this process. MPC transports into mitochondria, linking cytosolic glycolysis tricarboxylic acid cycle oxidative phosphorylation. Despite metabolic key function, of NSPCs has been addressed. quiescent have an active express high levels MPC. Pharmacological inhibition increases aspartate triggers NSPC activation. Furthermore, genetic Mpc1 ablation vitro vivo also activates NSPCs, which differentiate mature neurons, leading overall increased hippocampal neurogenesis aged mice. These findings highlight importance regulation identify pathway through import controls
Language: Английский
Citations
52
Signal Transduction and Targeted Therapy, Journal Year: 2025, Volume and Issue: 10(1)
Published: Feb. 18, 2025
Language: Английский
Citations
8
Scientific Reports, Journal Year: 2021, Volume and Issue: 11(1)
Published: July 15, 2021
Abstract Cell-to-cell communication is essential for the development and proper function of multicellular systems. We others demonstrated that tunneling nanotubes (TNT) proliferate in several pathological conditions such as HIV, cancer, neurodegenerative diseases. However, nature, function, contribution TNT to cancer pathogenesis are poorly understood. Our analyses demonstrate structures induced between glioblastoma (GBM) cells surrounding non-tumor astrocytes transfer tumor-derived mitochondria. The mitochondrial mediated by resulted adaptation tumor-like metabolism hypoxia conditions. In conclusion, an efficient cell-to-cell system used adapt microenvironment invasive nature tumor.
Language: Английский
Citations
65
Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13
Published: July 14, 2022
Macrophages are versatile immune cells associated with various diseases, and their phenotypes functions change on the basis of surrounding environments. Reprogramming metabolism is required for proper polarization macrophages. This review will focus basic metabolic pathways, effects key enzymes specific products, relationships between cellular macrophage in different diseases potential prospect therapy targeted enzymes. In particular, types characteristics macrophages at maternal-fetal interface a successful conception be discussed.
Language: Английский
Citations
43
Advanced Science, Journal Year: 2024, Volume and Issue: 11(13)
Published: Jan. 21, 2024
Abstract The effects of calcium phosphate (CaP) materials on macrophage polarization state vary with their physicochemical properties. study aims to elucidate the impact ion‐mediated energy metabolism M2 and corresponding regulatory mechanism. ions released from CaP ceramic as bioenergetic factor is identified; its concentration closely associated polarized state. After being taken up by sodium‐dependent transporter 1, extracellular produce via oxidative phosphorylation facilitating tricarboxylic acid flux, thereby contributing polarization. Further mechanistic analysis reveals that elevation basis can drive AMP‐activated protein kinase‐mammalian target rapamycin (AMPK‐mTOR) axis. Another effect adenosine triphosphate (ATP), a signaling molecule. Intracellular ATP into space degraded adenosine, which serves molecule through A2b receptor activate cyclic monophosphate (cAMP) pathway, promoting Overall, these findings may transform existing knowledge cell homeostasis bystanders pivotal factors guiding have implications for future design biomimetic scaffolds.
Language: Английский
Citations
9
Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(3), P. 754 - 754
Published: Jan. 24, 2025
Blast trauma presents a unique challenge due to its complex mechanism of injury, which impacts the brain and other vital organs through overpressure waves internal bleeding. Severe blood loss leads an inadequate oxygen supply insufficient fuel delivery cells, impairing ATP production by mitochondria—essential for cell survival. While clinical symptoms metabolic disruption are evident soon after molecular, cellular, systemic damage persists days years post-injury. Current challenges in treating traumatic injury (TBI) stem from (1) lack early blood-based biomarkers detecting failure mitochondrial (2) limited success mitochondrial-targeted therapeutic strategies. Objectives: To identify evaluating severity injuries investigate strategies targeting mitochondria. Methods: A preclinical rat model subjected blast exposure, with or without hemorrhagic shock (HS), followed resuscitation was utilized. Blood samples were obtained at baseline (T0), post-injury (T60), conclusion experiment (T180), analyzed using validated dipstick assay measure enzyme activity. Results: HS led significant decrease activity enzymes, including I, IV, pyruvate dehydrogenase (PDH), compared (p < 0.05). Concurrently, lactate concentrations significantly elevated 0.001). An inverse correlation observed between dysfunction levels Treatment sodium restored PDH near-baseline levels, corrected hyperlactatemia, reduced reactive species (ROS) Conclusions: Serial monitoring activity, such as PDH, may serve valuable tool prognostication guiding use therapies. Additionally, assays can provide insights into global redox status, potentially paving way novel interventions TBI.
Language: Английский
Citations
1
Nature, Journal Year: 2025, Volume and Issue: unknown
Published: March 5, 2025
Language: Английский
Citations
1
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2020, Volume and Issue: 1867(2), P. 166016 - 166016
Published: Nov. 25, 2020
Language: Английский
Citations
50
AJP Endocrinology and Metabolism, Journal Year: 2022, Volume and Issue: 323(1), P. E33 - E52
Published: May 30, 2022
Pyruvate metabolism, a central nexus of carbon homeostasis, is an evolutionarily conserved process and aberrant pyruvate metabolism associated with contributes to numerous human metabolic disorders including diabetes, cancer, heart disease. As product glycolysis, primarily generated in the cytosol before being transported into mitochondrion for further metabolism. entry mitochondrial matrix critical step efficient generation reducing equivalents ATP biosynthesis glucose, fatty acids, amino acids from pyruvate. However, many years, identity carrier protein(s) that remained mystery. In 2012, molecular-genetic identification (MPC), heterodimeric complex composed protein subunits MPC1 MPC2, enabled studies shed light on physiological processes regulated by A better understanding mechanisms regulating transport affected may enable novel therapeutics modulate flux treat variety disorders. Herein, we review our current knowledge MPC, discuss recent advances various tissue cell types, address some outstanding questions relevant this field.
Language: Английский
Citations
38
Life, Journal Year: 2022, Volume and Issue: 12(2), P. 280 - 280
Published: Feb. 14, 2022
Male infertility is a multifaceted disorder affecting approximately 50% of male partners in infertile couples. Over the years, has been diagnosed mainly through semen analysis, hormone evaluations, medical records and physical examinations, which course are fundamental, but yet inefficient, because 30% cases remain idiopathic. This dilemmatic status unknown needs to be addressed with more sophisticated result-driven technologies and/or techniques. Genetic alterations have linked infertility, thereby unveiling practicality investigating this from "omics" perspective. Omics aims at analyzing structure functions whole constituent given biological function different levels, including molecular gene level (genomics), transcript (transcriptomics), protein (proteomics) metabolites (metabolomics). In current study, an overview four branches omics their roles briefly discussed; potential usefulness assessing transcriptomic data understand pathology also elucidated. After publicly obtainable for datasets on total 1385 were retrieved, 10 met inclusion criteria used further analysis. These classified into groups according disease or cause infertility. The include non-obstructive azoospermia (NOA), obstructive (OA), (NOA OA), spermatogenic dysfunction, sperm Y chromosome microdeletion. Findings revealed that 8 genes (LDHC, PDHA2, TNP1, TNP2, ODF1, ODF2, SPINK2, PCDHB3) commonly differentially expressed between all groups. Likewise, 56 common NOA versus OA (ADAD1, BANF2, BCL2L14, C12orf50, C20orf173, C22orf23, C6orf99, C9orf131, C9orf24, CABS1, CAPZA3, CCDC187, CCDC54, CDKN3, CEP170, CFAP206, CRISP2, CT83, CXorf65, FAM209A, FAM71F1, FAM81B, GALNTL5, GTSF1, H1FNT, HEMGN, HMGB4, KIF2B, LDHC, LOC441601, LYZL2, PCDHB3, PGK2, PIH1D2, PLCZ1, PROCA1, RIMBP3, ROPN1L, SHCBP1L, SMCP, SPATA16, SPATA19, TEX33, TKTL2, TMCO2, TMCO5A, TSPAN16, TSSK1B, TTLL2, UBQLN3). genes, particularly above-mentioned involved diverse processes such as germ cell development, spermatid differentiation, regulation proteolysis, spermatogenesis metabolic processes. Owing stage-specific expression these any mal-expression can ultimately lead Therefore, currently available relating fertility identify biomarkers diagnosing potentially help unravelling some idiopathic cases.
Language: Английский
Citations
30