Comprehensive Genomic Analysis of Trihelix Transcription Factor Genes and Their Expression Underlying Abiotic Stress in Euphrates Poplar (Populus euphratica)

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 662 - 662

Published: Feb. 21, 2025

Trihelix transcription factors (TTFs) are light-sensitive proteins characterized by a triple-helix structure that play crucial role in regulating plant growth and development, especially response to abiotic stressors, such as drought salinity. This intriguing family of has been the focus extensive functional studies across various species. Despite their recognized significance, trihelix Populus euphratica not thoroughly explored, warranting more attention. study identifies 35 full-length genes euphratica, which grouped into five categories (GT-1, GT-γ, GT-2, SIP1, SH4) based on conserved motifs structural similarities, these unevenly distributed 19 linkage groups chromosomes. A syntenic analysis was conducted P. comparing it other The promoters contain numerous stress-responsive cis-elements, indicating potential for respond stress. RT-qPCR discovered significant induction gene salt stress, with 21 PeuTTF exhibiting distinct expression levels under conditions responsive Notably, heightened PeuTTF6, PeuTTF9, PeuTTF20 observed both roots leaves during suggesting TTF is connected plant’s conditions. Additionally, increases were noted PeuTTF2, PeuTTF31, PeuTTF32, may be convoluted These discoveries highlight improving tolerance plants. We offer new perspectives evolutionary trends variants we establish groundwork understanding properties salt-stressed drought-stressed provides opportunities advancement desert poplar agriculture have wider ramifications tree breeding techniques targeted at performance durability, particularly dry areas.

Language: Английский

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OsASR6 Enhances Salt Stress Tolerance in Rice

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(16), P. 9340 - 9340

Published: Aug. 19, 2022

High salinity seriously affects crop growth and yield. Abscisic acid-, stress-, ripening-induced (ASR) proteins play an important role in plant responses to multiple abiotic stresses. In this study, we identified a new salt-induced ASR gene rice (OsASR6) functionally characterized its mediating salt tolerance. Transcript levels of OsASR6 were upregulated under stress, H2O2 abscisic acid (ABA) treatments. Nuclear cytoplasmic localization the protein confirmed. Meanwhile, transactivation activity assay yeast demonstrated no self-activation ability. Furthermore, transgenic plants overexpressing showed enhanced oxidative stress tolerance as result reductions H2O2, malondialdehyde (MDA), Na/K relative electrolyte leakage. contrast, RNAi lines opposite results. A higher ABA content was also measured compared with control. Moreover, OsNCED1, key enzyme biosynthesis, found interact OsASR6. Collectively, these results suggest that serves primarily functional protein, enhancing representing candidate for genetic manipulation salinity-resistant rice.

Language: Английский

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NAC domain transcription factor gene GhNAC3 confers drought tolerance in plants

Plant Physiology and Biochemistry, Journal Year: 2023, Volume and Issue: 195, P. 114 - 123

Published: Jan. 6, 2023

Language: Английский

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Transcriptomic and metabolic regulatory network characterization of drought responses in tobacco

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 13

Published: Jan. 18, 2023

Drought stress usually causes huge economic losses for tobacco industries. exhibits multifaceted impacts on systems through inducing changes at different levels, such as physiological and chemical changes, of gene transcription metabolic changes. Understanding how plants respond adapt to drought helps generate engineered with enhanced resistance. In this study, we conducted multiple time point-related physiological, biochemical,transcriptomic assays using K326 its derived mutant 28 (M28) contrasting tolerance. Through integrative analyses transcriptome metabolome,we observed dramatic expression profiles between M28 before after treatment. found that some DEGs function key enzymes responsible ABA biosynthesis pathway, thereby mitigating impairment signaling dependent pathways. Four were involved in nitrogen metabolism, leading synthesis glutamate (Glu) starting from NO-3 /NO-2 serves an indicator responses. Importantly, regulatory network analyses, detected several induced TFs regulate genes network, indicating direct indirect involvement responses tobacco. Thus, our study sheds mechanistic insights into plant responding transcriptomic It also provides TF or non-TF candidates engineering manipulation breeding new varieties

Language: Английский

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The hidden harmony: Exploring ROS-phytohormone nexus for shaping plant root architecture in response to environmental cues

Plant Physiology and Biochemistry, Journal Year: 2023, Volume and Issue: 206, P. 108273 - 108273

Published: Dec. 12, 2023

Language: Английский

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