ZFP1 is a biomarker related to poor prognosis and immunity in gastric cancer DOI Creative Commons

Jibin Yao,

Fubin Ma,

Donghai Shi

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Sept. 11, 2024

We aimed to determine the prognostic significance of ZFP1 in gastric cancer (GC), its role immune microenvironment, and potential as a therapeutic target using data from The Cancer Genome Atlas (TCGA) database. overexpression was closely associated with tumour T stage histological grade. Patients GC high expression had poor outcomes. Lower longer symptom-free intervals disease-specific survival. Subgroup analyses T3 T4, N0, N1, M0 patients showed that overall survival (OS), survival, progression-free interval (PFI) were worse those expression. moderately strongly positively correlated infiltration levels effector central memory cells helper negatively Th17 NK CD56bright cells. lncRNA-miRNA-ZFP1 axis predicted public CCK8, colony formation, wound healing assays conducted investigate whether promoted proliferation migration Our study suggests plays key prognosis, response, progression is significant factor diagnosis treatment this disease.

Language: Английский

LncRNAs: the art of being influential without protein DOI Creative Commons
Lorena Ramírez Gonzales, Suze Blom, Rossana Henriques

et al.

Trends in Plant Science, Journal Year: 2024, Volume and Issue: 29(7), P. 770 - 785

Published: Feb. 16, 2024

The plant long noncoding (lnc)RNA field is on the brink of transitioning from large-scale identification lncRNAs to their functional characterization. Due cross-kingdom conservation interaction types and molecular functions, there much be learned mammalian lncRNA research. Here, we discuss different processes involving regulation chromatin splicing. Furthermore, interactome, which includes proteins, other RNAs, DNA. We explore how functionalities could reflected in similar pathways plants hypothesize that several breakthroughs research lead discovery novel functions. Expanding our knowledge biological role multiple applications paves way for future agricultural applications.

Language: Английский

Citations

17
Behind the scenes: How RNA orchestrates the epigenetic regulation of gene expression DOI Creative Commons
Arianna Mangiavacchi, Gabriele Morelli, Valerio Orlando

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: Jan. 25, 2023

Non-coding DNA accounts for approximately 98.5% of the human genome. Once labeled as “junk DNA”, this portion genome has undergone a progressive re-evaluation and it is now clear that some its transcriptional products, belonging to non-coding RNAs (ncRNAs), are key players in cell regulatory networks. A growing body evidence demonstrates crucial impact ncRNAs on mammalian gene expression. Here, we focus defined relationship between chromatin-interacting RNAs, particularly long RNA (lncRNA), enhancer (eRNA), natural antisense transcript (ncNAT), circular (circRNA) epigenome, common ground where both protein species converge regulate cellular functions. Through several examples, review provides an overview variety targets, interactors, mechanisms involved RNA-mediated modulation loci-specific epigenetic states, fundamental evolutive strategy orchestrate expression timely reversible manner. We will discuss how regulation impacts development tissue homeostasis alteration contributes onset progression many different diseases, cancer.

Language: Английский

Citations

37
Integrative Single-Cell Analysis Reveals Transcriptional and Epigenetic Regulatory Features of Clear Cell Renal Cell Carcinoma DOI Creative Commons
Zhenyuan Yu, Yufang Lv, Cheng Su

et al.

Cancer Research, Journal Year: 2023, Volume and Issue: 83(5), P. 700 - 719

Published: Jan. 6, 2023

Abstract Clear cell renal carcinoma (ccRCC) frequently features a high level of tumor heterogeneity. Elucidating the chromatin landscape ccRCC at single-cell could provide deeper understanding functional states and regulatory dynamics underlying disease. Here, we performed RNA sequencing (scRNA-seq) assay for transposase-accessible using (scATAC-seq) on 19 samples, whole-exome was used to understand heterogeneity between individuals. Single-cell transcriptome accessibility maps were constructed reveal characteristics different subtypes in ccRCC. Two long noncoding RNAs (RP11-661C8.2 CTB-164N12.1) identified that promoted invasion migration ccRCC, which validated with vitro experiments. Taken together, this study comprehensively characterized gene expression DNA regulation new insights into biology treatment Significance: A comprehensive analysis scATAC-seq scRNA-seq reveals programs level.

Language: Английский

Citations

33
Non-coding RNAs as therapeutic targets in cancer and its clinical application DOI Creative Commons

Xuejiao Leng,

Mengyuan Zhang,

Yujing Xu

et al.

Journal of Pharmaceutical Analysis, Journal Year: 2024, Volume and Issue: 14(7), P. 100947 - 100947

Published: Feb. 8, 2024

Cancer genomics has led to the discovery of numerous oncogenes and tumor suppressor genes that play critical roles in cancer development progression. Oncogenes promote cell growth proliferation, whereas inhibit division. The dysregulation these can lead cancer. Recent studies have focused on non-coding RNAs (ncRNAs), including circular RNA (circRNA), long (lncRNA), microRNA (miRNA), as therapeutic targets for In this article, we discuss ncRNAs associated with different types their potential targets. Here, highlight mechanisms action clinical applications treatment. Understanding molecular underlying identifying specific are essential steps towards effective treatments.

Language: Английский

Citations

13
Long non-coding RNAs in cancer: multifaceted roles and potential targets for immunotherapy DOI
Lokesh Kadian, Deepika Verma, Neelam Lohani

et al.

Molecular and Cellular Biochemistry, Journal Year: 2024, Volume and Issue: 479(12), P. 3229 - 3254

Published: Feb. 28, 2024

Language: Английский

Citations

9
The ceRNA network regulates epithelial-mesenchymal transition in colorectal cancer DOI Creative Commons
Ruina Li, Hui Xu, Xiaoling Gao

et al.

Heliyon, Journal Year: 2023, Volume and Issue: 9(3), P. e14143 - e14143

Published: March 1, 2023

Epithelial-mesenchymal transition (EMT) is a biological process that transforms epithelial cells into mesenchymal phenotype, conferring cell migration and invasion capabilities. EMT involved in the progression metastasis of colorectal cancer (CRC). Recently, emerging evidence has shown dysregulation non-coding RNA (ncRNA) was linked to EMT. ncRNAs, including long (lncRNA), regulate transcription downstream target genes (mRNA) through interaction with microRNAs (miRNAs); these are termed competitive endogenous (ceRNA) networks. CeRNA dysregulation-induced EMT, which prognosis CRC, attracted wide attention. However, understanding role regulation ceRNA network CRC remains limited. We discuss molecular functions lncRNA, networks related miRNAs mRNAs as well factors, such zinc finger E-box binding homeobox 1/2 (ZEB1/2), SNAIL, SLUG, TWIST1/2. In addition, lncRNAs directly genes, thereby initiating different signaling pathways promote were summarized. Clarifying molecules critical for mechanisms exploring potential therapeutic targets CRC.

Language: Английский

Citations

18
Non-coding RNAs in the pathophysiology of heart failure with preserved ejection fraction DOI Creative Commons

Elisabeth A. Jalink,

Amber W. Schonk, Reinier A. Boon

et al.

Frontiers in Cardiovascular Medicine, Journal Year: 2024, Volume and Issue: 10

Published: Jan. 8, 2024

Heart failure with preserved ejection fraction (HFpEF) is the largest unmet clinical need in cardiovascular medicine. Despite decades of research, treatment option for HFpEF still limited, indicating our ongoing incomplete understanding on underlying molecular mechanisms. Non-coding RNAs, comprising microRNAs (miRNAs), long non-coding RNAs (lncRNAs) and circular (circRNAs), are non-protein coding RNA transcripts, which implicated various diseases. However, their role pathogenesis unknown. Here, we discuss miRNAs, lncRNAs circRNAs that involved pathophysiology HFpEF, namely microvascular dysfunction, inflammation, diastolic dysfunction cardiac fibrosis. We interrogated evidence dissected mechanisms ncRNAs by looking at relevant vivo vitro models mimic co-morbidities patients HFpEF. Finally, potential as biomarkers novel therapeutic targets future treatment.

Language: Английский

Citations

8
lncRNA-mediated ceRNA network in bladder cancer DOI Creative Commons
Kun Li,

Tongyue Yao,

Ziqiang Wang

et al.

Non-coding RNA Research, Journal Year: 2022, Volume and Issue: 8(2), P. 135 - 145

Published: Dec. 14, 2022

Bladder cancer is a common disease associated with high rates of morbidity and mortality. Although immunotherapy approaches such as adoptive T-cell therapy immune checkpoint blockade have been investigated for the treatment bladder cancer, their off-target effects ability to affect only single targets led clinical outcomes that are far from satisfactory. Therefore, it important identify novel can effectively control tumor growth metastasis. It well known long noncoding RNAs (lncRNAs) powerful regulators gene expression. Increasing evidence has shown dysregulated lncRNAs in involved cell proliferation, migration, invasion, apoptosis, epithelial-mesenchymal transition (EMT). In this review, we focus on roles underlying mechanisms lncRNA-mediated competing endogenous RNA (ceRNA) networks regulation progression. addition, discuss potential targeting ceRNA overcome resistance its association clinicopathological features patients. We hope review will stimulate research develop more effective therapeutic treatment.

Language: Английский

Citations

24
Epigenetic mechanism of RBM15 in affecting cisplatin resistance in laryngeal carcinoma cells by regulating ferroptosis DOI Creative Commons

Yue Liang,

Haoyue Zhong,

Yi Zhao

et al.

Biology Direct, Journal Year: 2024, Volume and Issue: 19(1)

Published: July 23, 2024

Abstract Laryngeal carcinoma (LC) is a common cancer of the respiratory tract. This study aims to investigate role RNA-binding motif protein 15 (RBM15) in cisplatin (DDP) resistance LC cells. LC-DDP-resistant cells were constructed. RBM15, lysine-specific demethylase 5B (KDM5B), lncRNA Fer-1 like family member 4 (FER1L4), KCNQ1 overlapping transcript 1 (KCNQ1OT1), glutathione peroxidase (GPX4), and Acyl-CoA synthetase long-chain (ACSL4) was examined. Cell viability, IC 50 , proliferation assessed after RBM15 downregulation. The enrichment insulin-like growth factor 2 mRNA-binding 3 (IGF2BP3) N6-methyladenosine (m6A) on KDM5B analyzed. mRNA stability measured actinomycin D treatment. A tumor xenograft assay conducted verify LC. Results showed that upregulated its knockdown decreased cell proliferation, glutathione, iron ion content, ROS, malondialdehyde, ACSL4, ferroptosis. Mechanistically, improved an IGF2BP3-dependent manner, resulting FER1L4 downregulation GPX4 upregulation. increased KCNQ1OT1 inhibited ACSL4. KDM5B/KCNQ1OT1 overexpression or promoted DDP by inhibiting In conclusion, expression, upregulation ferroptosis downregulating upregulating GPX4, as well Silencing vivo.

Language: Английский

Citations

5
Role of oncogenic long noncoding RNA KCNQ1OT1 in colon cancer DOI Creative Commons

GANG LIU,

Lei Shi, Bin Wang

et al.

Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics, Journal Year: 2024, Volume and Issue: 32(3), P. 585 - 596

Published: Jan. 1, 2024

The role of lncRNA KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) in colon cancer involves various tumorigenic processes and has been studied widely. However, the mechanism by which it promotes remains unclear. Retroviral vector pSEB61 was retrofitted established HCT116-siKCN SW480-siKCN cells to silence KCNQ1OT1. Cellular proliferation measured using CCK8 assay, flow cytometry (FCM) detected cell cycle changes. RNA sequencing (RNA-Seq) analysis showed differentially expressed genes (DEGs). Gene ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses were carried out analyze enriched functions signaling pathways. RT-qPCR, immunofluorescence, western blotting validate downstream gene expressions. effects tumorigenesis evaluated BALB/c nude mice tumor xenografts. Our data revealed that silencing KCNQ1OT1 HCT116 SW480 slowed growth decreased number G2/M phase. RNA-Seq DEGs GO KEGG pathways such as DNA replication cycle. confirmed CCNE2 PCNA significantly suppressed mice. study suggests may provide a promising therapeutic strategy for cancer.

Language: Английский

Citations

4