Exploring the connection between climatic conditions and genetic properties of Abies alba Mill., including warm and summer-dry Mediterranean environments

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 964, P. 178559 - 178559

Published: Jan. 23, 2025

Abies alba Mill. is a prominent European tree species predominantly inhabiting cool and humid montane environments. However, paleoecological evidence reveals that during the Eemian mid-Holocene, A. thrived in much warmer drier climates. This capacity nowadays reflected cryptic meso- sub-Mediterranean lowland populations. To link populations across diverse climates spanning from Mediterranean lowlands to Alpine timberline, we genotyped 421 specimens Italy Switzerland at 190 single-nucleotide polymorphisms (SNPs). Population genetic structure analyses indicate isolated Tuscany Ticino align genetically with higher elevation each region, suggesting their thrive warmer, conditions cannot be attributed plantations planting stock originating different or occurrence of single warm-adapted lineage showing disjunct distribution, unless migration between stands occurred. Genotype-environment associations reveal temperature seasonality, precipitation critical seasons, relative humidity are important for explaining species' variation. With genotype-environment genotype-phenotype associations, identified candidate adaptive genes potentially linked climatic drought response. While certain alleles may have spread Southern could explained by shared ancestry Tuscan populations, local adaptation occurred specific loci. These findings underscore importance considering hitherto overlooked better understand niche its potential forest conservation management under global warming.

Language: Английский

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Implication of ribosomal protein in abiotic and biotic stress

Planta, Journal Year: 2025, Volume and Issue: 261(4)

Published: March 11, 2025

Language: Английский

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Exploring Morphological, Transcriptomic, and Metabolomic Differences Between Two Sister Lines with Contrasting Resistance to Orange Rust Disease in Sugarcane

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3490 - 3490

Published: April 8, 2025

Sugarcane (Saccharum spp.) hybrid, one of the most important crops in Florida, has been affected by orange rust (OR) disease caused Puccinia kuehnii since 2007, resulting significant yield loss. Developing resistant cultivars to this become an goal sugarcane breeding programs. However, specific genes and molecular mechanisms underlying resistance OR are still not clear. In study, we selected two sister lines with different genotypes—showing contrasting responses disease—from a major quantitative trait loci (QTL) region controlling resistance. Morphological anatomical observations revealed that line (540) had significantly smaller stomatal size lower density than susceptible (664). Transcriptomic analyses showed 540 increased cell surface modification activity, suggesting possible receptors. Differentially expressed gene coexpression also key involved biosynthesis anti-fungal molecules, such as hordatines, arabidopyrones, alkaloids. They strong increase long non-coding RNA expression, playing role transcriptional regulation. Transcriptomic–metabolomic joint analysis suggested phenylpropanoid derivatives purported antioxidant capabilities 540, especially those deriving from ferulate. Genes, pathways, some single-nucleotide polymorphisms identified study will provide fundamental information resources advance knowledge genetic relation disease, supporting programs developing improved OR.

Language: Английский

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Proteomic Analysis of the Papachloya-Fusarium equiseti Interaction: Understanding Mode of Infection and Plant Response at the Molecular Level

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102583 - 102583

Published: Jan. 1, 2025

Language: Английский

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Comparative Proteomic Analysis of Popcorn Genotypes Identifies Differentially Accumulated Proteins Associated with Resistance Pathways to Southern Leaf Blight Disease

Plants, Journal Year: 2025, Volume and Issue: 14(3), P. 426 - 426

Published: Feb. 1, 2025

Southern leaf blight (SLB), caused by Bipolaris maydis, poses a significant threat to maize and popcorn production. To understand the molecular mechanisms underlying SLB resistance, we conducted high-throughput proteomic analysis comparing SLB-resistant (L66) SLB-susceptible (L51) genotypes at four ten days after inoculation (DAI). A total of 717 proteins were identified, with 151 differentially accumulated (DAPs) between genotypes. Eighteen DAPs exhibited same regulatory pattern in both (R4/S4) (R10/S10) DAI. The protein-protein interaction (PPI) network linked resistance susceptibility enriched specific metabolic pathways response, including photosynthesis, ribosome, ascorbate aldarate metabolism, glutathione carbon metabolism. Proteins such as photosystem II 11 kD protein (B4FRJ4, PSB27-1), which was up-regulated time points (R4/S4 R10/S10), 60S acidic ribosomal P0 (A0A1D6LEZ7, RPP0B), unique resistant genotype (R4 R10), highlighted importance maintaining photosynthetic efficiency synthesis during pathogen attack. Additionally, dehydroascorbate reductase like-3 (B4F817, DHAR3) consistently genotypes, emphasizing its role redox balance ROS detoxification. In contrast, glyceraldehyde-3-phosphate dehydrogenase (K7UGF5, GAPC2), glycolytic enzyme, susceptible genotype, suggesting involvement managing energy metabolism under stress conditions. Our findings suggest that involves combination enhanced repair, homeostasis, activity, providing new potential targets, DHAR3 RPP0B, for genetic improvement resistance. These results offer valuable insights into breeding programs aimed developing varieties.

Language: Английский

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Physiological, Epigenetic, and Transcriptome Analyses Provide Insights into the Responses of Wheat Seedling Leaves to Different Water Depths under Flooding Conditions

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(23), P. 16785 - 16785

Published: Nov. 26, 2023

Flooding stress, including waterlogging and submergence, is one of the major abiotic stresses that seriously affects growth development plants. In present study, physiological, epigenetic, transcriptomic analyses were performed in wheat seedling leaves under (WL), half submergence (HS), full (FS) treatments. The results demonstrate FS increased leaves’ hydrogen peroxide (H2O2) malondialdehyde (MDA) contents reduced their chlorophyll (SPAD), photosynthetic efficiency (Fv/Fm), shoot dry weight more than HS WL. addition, catalase (CAT) peroxidase (POD) activities However, there no significant differences H2O2, MDA, SPAD, Fv/Fm, superoxide dismutase (SOD) POD between WL changes DNA methylation related to stress types, increasing treatments decreasing treatment. Additionally, a total 9996, 10,619, 24,949 genes differentially expressed WL, HS, treatments, respectively, among which ‘photosynthesis’, ‘phenylpropanoid biosynthesis’, ‘plant hormone signal transduction’ pathways extensively enriched three flooding involved these showed flooding-type-specific expression. Moreover, responses observed conditions, enrichment specific TFs response pathways. These will contribute better understanding molecular mechanisms underlying provide valuable genetic epigenetic information for breeding flood-tolerant varieties wheat.

Language: Английский

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Unraveling the Mechanisms of Efficient Phosphorus Utilization in Popcorn (Zea mays L. var. everta): Insights from Proteomic and Metabolite Analysis

Journal of Proteome Research, Journal Year: 2024, Volume and Issue: 23(8), P. 3108 - 3123

Published: April 22, 2024

The expansion of agriculture and the need for sustainable practices drives breeders to develop plant varieties better adapted abiotic stress such as nutrient deficiency, which negatively impacts yields. Phosphorus (P) is crucial photosynthesis growth, but its availability in soil often limited, hampering crop development. In this study, we examined response two popcorn inbred lines, L80 P7, have been characterized previously P-use inefficient efficient, respectively, under low (stress) high P (control) availability. Physiological measurements, proteomic analysis, metabolite assays were performed unravel physiological molecular responses associated with efficient use popcorn. We observed significant differences protein abundances supply between lines. A total 421 differentially expressed proteins (DEPs) 436 DEPs P7. These involved photosynthesis, biosynthesis, biosynthesis secondary metabolites, energy metabolism. addition, flavonoids accumulated higher abundance Our results help us understand major components utilization popcorn, providing new insights breeding programs.

Language: Английский

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RNA-Seq Bulked Segregant Analysis of an Exotic B. napus ssp. napobrassica (Rutabaga) F2 Population Reveals Novel QTLs for Breeding Clubroot-Resistant Canola

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(9), P. 4596 - 4596

Published: April 23, 2024

In this study, a rutabaga (

Language: Английский

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Ribosomal Proteins in Ribosome Assembly

Biomolecules, Journal Year: 2024, Volume and Issue: 15(1), P. 13 - 13

Published: Dec. 26, 2024

Ribosomes are the cellular machinery responsible for translating mRNA into proteins, a process fundamental to all domains of life from bacteria eukaryotes [...].

Language: Английский

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Efficient assembly of plant genomes: A case study with evolutionary implications inRanunculus(Ranunculaceae)

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 11, 2023

Abstract Currently, it is still a challenge - in terms of laboratory effort and cost, as well assembly quality to unravel the sequence large complex genomes from non-model/crop plants. This often hampers study evolutionarily intricate species groups. The species-rich genus Ranunculus (Ranunculaceae) an important angiosperm group for polyploidy, apomixis, reticulate evolution, biogeography. However, neither mitochondrial, nor high-quality nuclear genome sequences are available. limits phylogenomic, functional, taxonomic analyses thus far. Here, we tested Illumina short- read, Oxford Nanopore Technology (ONT) or PacBio/HiFi long-read, hybrid-read strategies. We used diploid progenitor R. cassubicifolius ( auricomus complex), selected best assemblies completeness, contiguity, scores. first assembled plastome (156 kbp, 85 genes) mitogenome (1.18 Mbp, 40 using Illumina-PacBio-hybrid strategies, respectively. also present updated phylogeny Ranunculaceae, including studies gene loss (e.g., infA , ycf15 rps ) with evolutionary implications. For genome, favored PacBio-based three-times polished filtered reads subsequently scaffolded into 8 pseudochromosomes by chromatin conformation data (Hi-C) representative sequence. obtained haploid 2.69 Gbp, 94.1% complete BUSCO ‘embryophyta_odb10’ genes found, 31,322 annotated genes. genomic information presented here will improve phylogenomic this complex, enable advanced evolutionary, biogeographic beyond Ranunculaceae future. Significance Statement model system flowering plants biogeography research. mitochondrial sequence, plastid evolution Ranunculaceae. Using Illumina, ONT, PacBio data, developed efficient strategy that can be applied other non-model Results useful improving population analyses, better functional at species, family level.

Language: Английский

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