Advances in Clinical Medicine, Journal Year: 2024, Volume and Issue: 14(04), P. 1218 - 1223
Published: Jan. 1, 2024
Language: Английский
Journal of Orthopaedic Surgery and Research, Journal Year: 2025, Volume and Issue: 20(1)
Published: March 18, 2025
Differentiation of bone marrow mesenchymal stem cells (BMSCs) is pivotal in the pathogenesis steroid-induced femoral head osteonecrosis. Icariin, an active ingredient Epimedii herba, has potential to regulate osteogenic differentiation BMSCs. Nevertheless, related mechanism still unclear. The study aimed explore whether icariin can affect by activating PI3K/AKT signaling alter UTX and EZH2 expression thus regulating osteogenesis-related genes BMSCs were collected from Sprague Dawley rats identified measuring positive ratios cell markers using flow cytometry. Cells treated with 1 μmol/L dexamethasone (DEX) for 24 h or without 0.1–10 μM treatment. Cell counting Kit-8 (CCK-8) assays cytometry analyses performed measure viability apoptosis. Western blotting was conducted measurement apoptotic markers, factors involved PI3K/AKT-UTX/EZH2 pathway, adipogenesis-related factors. Alizarin red S staining Oil-red O effect DEX, icariin, overexpression, knockdown on adipogenic Icariin ameliorated DEX-induced rat BMSC injury. activated signaling, thereby upregulating phosphorylated levels while inhibiting H3K27me3 expression. Additionally, promoted inhibited Importantly, overexpressing silencing exerted similar effects as did. promotes DEX-treated upregulate inhibit EZH2, finally inducing depletion.
Language: Английский
Citations
0
Cells, Journal Year: 2024, Volume and Issue: 13(9), P. 776 - 776
Published: May 1, 2024
We investigated the impact of mesenchymal stem cell (MSC) therapy on treating bilateral human hip osteonecrosis, analyzing 908 cases. This study assesses factors such as tissue source and count, comparing core decompression with various therapies. research emphasizes bone repair according to pre-treatment conditions specificities in osteonecrosis repair, indicating a potential for improved strategies hips without femoral head collapse. utilized single-center retrospective analysis investigate efficacy cellular approaches osteonecrosis. It examined (autologous marrow concentrate, allogeneic expanded, autologous expanded), quantity (from none alone millions therapy), stage volume. Excluding collapse, it focused patients who had both pre-operative post-operative MRIs, follow-up over five years. The divided these into seven groups based match control treatment variations primarily investigating outcomes between decompression, washing effect, different sources MSCs. Younger (<30 years) demonstrated significantly better volumes, particularly II lesions, than older counterparts. Additionally, volume increased number implanted MSCs up 1,000,000, beyond which no additional benefits were observed. No significant difference was observed (BMAC, allogenic, or expanded cells). also highlighted that ‘washing effect’ beneficial, larger-volume when combined decompression. Partial more frequent event observed, while total rare. alongside injected cells, affected outcomes. In summary, this provides comprehensive insights effectiveness variables influencing use cells acknowledging complexity variability results age, disease stage.
Language: Английский
Citations
1
Biochemical Genetics, Journal Year: 2024, Volume and Issue: unknown
Published: May 28, 2024
Language: Английский
Citations
1
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: Nov. 13, 2024
Circular RNA (circRNA) are a unique class of non-coding RNAs characterized by their covalently closed loop structures, which grant them properties such as stability and conservation. Among these, circFOXP1 has been implicated in various diseases, including cancers, respiratory, skeletal, cardiovascular disorders. This review systematically examines circFOXP1’s role disease progression, highlighting its involvement critical biological processes, cell proliferation, invasion, apoptosis, autophagy. Mechanistically, functions through miRNA sponging, protein interactions, modulation key signaling pathways Wnt PI3K/AKT. We discuss potential diagnostic therapeutic target. Our analysis also identifies unresolved questions, the precise regulatory networks involving translation potential, offering for future research.
Language: Английский
Citations
1
Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 10383 - 10396
Published: Dec. 1, 2024
Background: Corticosteroids provide well-established therapeutic benefits; however, they are also accompanied by adverse effects on bone. Metformin is a widely used medication for managing type 2 diabetes mellitus. Recent studies have highlighted additional benefits of metformin, particularly concerning bone health and oxidative stress. Objective: This research investigates the prednisolone cellular metabolic functions formation using 3D in vitro model. Then, we demonstrate potential metformin stress calcified matrix due to corticosteroids. Methods: Human mesenchymal stem cells (MSCs) macrophages were cultured GelMA scaffold stimulated with prednisolone, without metformin. The metformin's effect(s) assessed analyzing cell viability, osteogenesis markers, mineralization, inflammatory markers. Oxidative was measured evaluating reactive oxygen species (ROS) levels ATP production. Results: Prednisolone exhibited cytotoxic effects, reducing viability MSCs macrophages. Lower detected MSC group. positively affected functions, including enhanced osteoblast activity increased mineralization. Furthermore, effectively reduced stress, as evidenced decreased ROS These findings indicate that protects against damage, thus supporting osteogenesis. Conclusion: exhibits promising beyond its role management. capacity alleviate highlights environments. Keywords: corticosteroid, osteogenesis, inflammation, bone,
Language: Английский
Citations
1
Advances in Clinical Medicine, Journal Year: 2024, Volume and Issue: 14(04), P. 1218 - 1223
Published: Jan. 1, 2024
Language: Английский
Citations
0