Single-step 3D bioprinting of alginate-collagen I hydrogel fiber rings to promoter angiogenic network formation DOI
Ying Betty Li,

Marina Rukhlova,

Dongling Zhang

et al.

Tissue Engineering Part C Methods, Journal Year: 2024, Volume and Issue: 30(7), P. 289 - 306

Published: July 1, 2024

In the advent of tissue engineering and regenerative medicine, demand for innovative approaches to biofabricate complex vascular structures is increasing. We describe a single-step 3D bioprinting method leveraging Aspect Biosystems RX1 technology, which integrates crosslinking step at flow-focusing junction, immortalized adult rat brain endothelial cell (SV-ARBEC)-encapsulated alginate-collagen type I hydrogel rings. This biofabrication process involves strategic layer-by-layer assembly rings, encapsulating SV-ARBECs in spatially controlled manner while optimizing access media nutrients. The spatial arrangement within rings promotes spontaneous angiogenic network formation constrained deposition cells matrix facilitates tissue-like organized vascular-like development. approach provides platform that can be adapted many different types leveraged better understand mechanisms driving angiogenesis vascular-network bioprinted constructs supporting development more disease models advancing drug discovery, engineering, medicine applications.

Language: Английский

Recent advancements and challenges in 3D bioprinting for cancer applications DOI
Swayam Aryam Behera, Binita Nanda, P. Ganga Raju Achary

et al.

Bioprinting, Journal Year: 2024, Volume and Issue: 43, P. e00357 - e00357

Published: Sept. 13, 2024

Language: Английский

Citations

1
Single-step 3D bioprinting of alginate-collagen I hydrogel fiber rings to promoter angiogenic network formation DOI
Ying Betty Li,

Marina Rukhlova,

Dongling Zhang

et al.

Tissue Engineering Part C Methods, Journal Year: 2024, Volume and Issue: 30(7), P. 289 - 306

Published: July 1, 2024

In the advent of tissue engineering and regenerative medicine, demand for innovative approaches to biofabricate complex vascular structures is increasing. We describe a single-step 3D bioprinting method leveraging Aspect Biosystems RX1 technology, which integrates crosslinking step at flow-focusing junction, immortalized adult rat brain endothelial cell (SV-ARBEC)-encapsulated alginate-collagen type I hydrogel rings. This biofabrication process involves strategic layer-by-layer assembly rings, encapsulating SV-ARBECs in spatially controlled manner while optimizing access media nutrients. The spatial arrangement within rings promotes spontaneous angiogenic network formation constrained deposition cells matrix facilitates tissue-like organized vascular-like development. approach provides platform that can be adapted many different types leveraged better understand mechanisms driving angiogenesis vascular-network bioprinted constructs supporting development more disease models advancing drug discovery, engineering, medicine applications.

Language: Английский

Citations

0