Highly Sensitive Amperometric Biosensors Based on Oxidases and CuCe Nanoparticles Coupled with Porous Gold DOI Creative Commons
Nataliya Stasyuk, Olha Demkiv, Galina Gayda

et al.

Published: Feb. 14, 2022

Metallic nanoparticles are usually applied in biosensors as catalysts and/or mediators of electron transfer. We describe the development amperometric (ABSs) based on oxidases and CuCe (nCuCe). nCuCe, being an electro-active mediator active peroxidase (PO) mimetic, was used H2O2-sensing platform oxidase-based ABSs. ABSs for glucose, primary alcohols, methyl amine, catechol, L-arginine, which corresponding were developed. These exhibited improved analytical characteristics comparison with appropriate bi-enzyme containing natural PO. Including electrodeposited porous gold chemo-sensing layer shown to increase significantly sensitivities all constructed

Language: Английский

Copper Hexacyanoferrates Obtained via Flavocytochrome b2 Assistance: Characterization and Application DOI Creative Commons
Galina Gayda, Olha Demkiv, Nataliya Stasyuk

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(3), P. 157 - 157

Published: March 2, 2025

Artificial enzymes or nanozymes (NZs) are gaining significant attention in biotechnology due to their stability and cost-effectiveness. NZs can offer several advantages over natural enzymes, such as enhanced under harsh conditions, longer shelf life, reduced production costs. The booming interest is likely continue potential applications expand. In our previous studies, we reported the “green” synthesis of copper hexacyanoferrate (gCuHCF) using oxidoreductase flavocytochrome b2 (Fcb2). Organic–inorganic micro-nanoparticles were characterized detail, including structure, composition, catalytic activity, electron-mediator properties. An SEM analysis revealed that gCuHCF possesses a flower-like structure well-suited for concentrating stabilizing Fcb2. As an effective peroxidase (PO) mimic, has been successfully employed H2O2 detection amperometric sensors oxidase-based biosensors. current study, demonstrated uniqueness lies its multifunctionality, serving PO chemosensor ammonium ions, biosensor L-lactate, exhibiting perovskite-like This exceptional ability enhance fluorescence blue light irradiation being first time. Using PO-like NZ, novel developed, optical L-arginine electrochemical biosensors methanol glycerol determination. Thus, gCuHCF, synthesized via Fcb2, presents promising platform development biosensors, bioreactors, biofuel cells, solar other advanced devices. innovative approach utilizing biocatalysts nanoparticle highlights groundbreaking direction materials science biotechnology.

Language: Английский

Citations

1
Improved reuse and storage performances at room temperature of a new environmentally friendly lactate oxidase biosensor prepared by ambient electrospray immobilization DOI Creative Commons
Mattea Carmen Castrovilli, Viviana Scognamiglio, Emanuela Tempesta

et al.

Green Chemistry, Journal Year: 2023, Volume and Issue: 25(13), P. 5257 - 5266

Published: Jan. 1, 2023

A new, environmentally friendly lactate oxidase (LOX) based biosensor for detection, with unprecedented reuse and storage capabilities at room temperature, has been manufactured using the ambient electrospray deposition (ESD) technique.

Language: Английский

Citations

9
Amperometric Biosensors for L-Arginine Determination Based on L-Arginine Oxidase and Peroxidase-Like Nanozymes DOI Creative Commons
Nataliya Stasyuk, Galina Gayda, Olha Demkiv

et al.

Applied Sciences, Journal Year: 2021, Volume and Issue: 11(15), P. 7024 - 7024

Published: July 29, 2021

There are limited data on amperometric biosensors (ABSs) for L-arginine (Arg) determination based oxidases that produce hydrogen peroxide (H2O2) as a byproduct of enzymatic reaction, and artificial peroxidases (POs) decomposition H2O2. The most frequently proposed Arg-sensitive oxidase-based ABSs contain at least two enzymes in the bioselective layer; this complicates procedure increases cost analysis. Therefore, construction one-enzyme ABS Arg analysis is practical problem. In current work, fabrication, characterization three types direct measurement were proposed. oxidase (ArgO) isolated from mushroom Amanita phalloides was co-immobilized with PO-like nanozymes (NZs) surface graphite electrodes. As PO mimetics, chemically synthesized NZs CeCu (nCeCU) NiPtPd (nNiPtPd), well green-synthesized hexacyanoferrate copper (gCuHCF), used. novel exhibited high sensitivity selectivity to Arg, broad linear ranges good storage stabilities. Two tested real samples products containing including pharmaceutical preparation “Tivortine”, juices, wine. A correlation (R = 0.995) demonstrated between results testing “Tivortine” juice using nCeCU/GE nNiPtPd/GE. It worth mentioning only slight difference (less than 1%) observed “Tivortin” experimentally determined content its value declared by producer. ArgO-NZ-based may be promising different branches science, medicine, industry.

Language: Английский

Citations

20
Nanomaterials as Redox Mediators in Laccase-Based Amperometric Biosensors for Catechol Assay DOI Creative Commons
Olha Demkiv, Galina Gayda, Nataliya Stasyuk

et al.

Biosensors, Journal Year: 2022, Volume and Issue: 12(9), P. 741 - 741

Published: Sept. 8, 2022

Laccase is a copper-containing enzyme that does not require hydrogen peroxide as co-substrate or additional cofactors for an enzymatic reaction. Nanomaterials of various chemical structures are usually applied to the construction enzyme-based biosensors. Metals, metal oxides, semiconductors, and composite NPs perform functions in electrochemical transformation schemes platform immobilization, mediator electron transfer, signal amplifier. We describe here development amperometric biosensors (ABSs) based on laccase redox-active micro/nanoparticles (hereafter-NPs), which were immobilized graphite electrode (GE). For this purpose, we isolated highly purified from fungus Trametes zonatus, then synthesized bi- trimetallic noble transition metals, well hexacyanoferrates (HCF) metals; these layered onto surfaces GEs. The electroactivity many GEs was characterized by cyclic voltammetry (CV) experiments. most effective mediators transfer selected laccase-based ABSs. As result, number catechol-sensitive ABSs constructed characterized. laccase/CuCo/GE demonstrated possess highest sensitivity catechol (4523 A·M-1·m-2) among tested proposed may be promising analysis phenolic derivatives real samples drinking water, wastewater, food products.

Language: Английский

Citations

12
Nanoparticles of prussian blue analogues as peroxidase mimetics for nanozyme – oxidase – based biosensors DOI Open Access
Olha Demkiv, Nataliya Stasyuk, N. M. Grynchyshyn

et al.

Biopolymers and Cell, Journal Year: 2024, Volume and Issue: 40(2), P. 96 - 108

Published: June 18, 2024

Keywords: Prussian blue analogues, nanozymes, artificial peroxidase, glucose oxidase, galactose amperometric biosensor

Language: Английский

Citations

2
Highly Porous 3D Gold Enhances Sensitivity of Amperometric Biosensors Based on Oxidases and CuCe Nanoparticles DOI Creative Commons
Nataliya Stasyuk, Olha Demkiv, Galina Gayda

et al.

Biosensors, Journal Year: 2022, Volume and Issue: 12(7), P. 472 - 472

Published: June 29, 2022

Metallic nanoparticles potentially have wide practical applications in various fields of science and industry. In biosensorics, they usually act as catalysts or nanozymes (NZs) mediators electron transfer. We describe here the development amperometric biosensors (ABSs) based on purified oxidases, synthesized CuCe (nCuCe), micro/nanoporous gold (pAu), which were electro-deposited a graphite electrode (GE). As an effective peroxidase (PO)-like NZ, nCuCe was used hydrogen-peroxide-sensing platform ABSs that glucose oxidase, alcohol methylamine L-arginine oxidase. At same time, is electroactive mediator has been laccase-based ABSs. result, we constructed characterized glucose, methanol, methyl amine, L-arginine, catechol, respectively. The developed nCuCe-based exhibited improved analytical characteristics comparison with corresponding PO-based Additionally, presence pAu, its extremely advanced chemo-sensing surface layer, shown to significantly increase sensitivities all example, bioelectrodes containing laccase/GE, laccase/nCuCe/GE, laccase/nCuCe/pAu/GE catechol at 2300, 5055, 9280 A·M

Language: Английский

Citations

10
Highly Sensitive Amperometric Sensor Based on Laccase-Mimicking Metal-Based Hybrid Nanozymes for Adrenaline Analysis in Pharmaceuticals DOI Open Access
Olha Demkiv, Nataliya Stasyuk, Galina Gayda

et al.

Catalysts, Journal Year: 2021, Volume and Issue: 11(12), P. 1510 - 1510

Published: Dec. 11, 2021

Nanozymes are nanomaterials which exhibit artificial enzymatic activities and considered as alternatives to natural enzymes. They characterized by good catalytic activity high stability, well ease low cost of preparation. In this study, the mimetics laccase or “nanolaccases” (NLacs) were synthesized a simple method chemical reduction transition metal salts. The NLacs tested for their in solution on electrode surface. most effective NLacs, namely nAuCePt nPtFe, found possess excellent laccase-like capable oxidizing endocrine hormone adrenaline (AD). These detail used development amperometric sensors AD determination. containing best fungal laccase, constructed. nAuCePt-containing sensor had specificity relation improved analytical characteristics. It possessed 384-fold higher sensitivity than (230,137 A·M−1·m−2), 64-fold lower limit detection (0.025 µM), broader linear range (0.085–45 µM) comparison with based laccase. constructed was successfully analysis pharmaceutical formulation.

Language: Английский

Citations

10
Peroxidase-like Nanoparticles of Noble Metals Stimulate Increasing Sensitivity of Flavocytochrome b2-Based L-Lactate Biosensors DOI Creative Commons
Galina Gayda, Olha Demkiv, Nataliya Stasyuk

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(11), P. 562 - 562

Published: Nov. 20, 2024

We report the development of amperometric biosensors (ABSs) employing flavocytochrome

Language: Английский

Citations

1
Self-assembled iridium(III) complex microspheres on the carbon paste electrode surface for signal enhanced amperometric determination of H2O2 in color cream developers DOI
Jasmina Anojčić,

Keerati Kullawanichaiyanan,

Sanja Mutić

et al.

Journal of Electroanalytical Chemistry, Journal Year: 2021, Volume and Issue: 904, P. 115873 - 115873

Published: Nov. 13, 2021

Language: Английский

Citations

4
Analysis of NMR Spectral Analysis and Molecular Electronic Potential Maps on L-Arginine Phosphate Crystals for Non-Linear Optical Applications DOI Open Access

Chandran Tamil Malar,

P. Kumaresan,

Subramanian Nithiyanatntham

et al.

Biointerface Research in Applied Chemistry, Journal Year: 2021, Volume and Issue: 12(6), P. 8353 - 8366

Published: Dec. 11, 2021

L-Arginine Phosphate (LAP) is a possible material for applications involving nonlinear optical properties. Slow evaporation was used to generate single LAP crystals with very high degree of transparency from an aqueous solution. The solubility the pure and doped measured at different temperatures in double-distilled water. Natural bond orbital (NBO) research investigated molecule's stability charge delocalization. HOMO-LUMO energies describe transfer between molecules. electrostatic potential molecules has been investigated. correlation found crystalline perfection SHG potency mentioned.

Language: Английский

Citations

1