A Review of the Mechanism, Properties, and Applications of Hydrogels Prepared by Enzymatic Cross-linking
Journal of Agricultural and Food Chemistry,
Journal Year:
2023,
Volume and Issue:
71(27), P. 10238 - 10249
Published: June 30, 2023
Hydrogels,
as
biological
materials,
are
widely
used
in
food,
tissue
engineering,
and
biomedical
applications.
Nevertheless,
many
issues
remain
the
preparation
of
hydrogels
by
physical
chemical
methods,
such
low
bioaffinity,
weak
mechanical
properties,
unstable
structures,
which
also
limit
their
applications
other
fields.
However,
enzymatic
cross-linking
method
has
advantages
high
catalytic
efficiency,
mild
reaction
conditions,
presence
nontoxic
substances.
In
this
review,
we
evaluated
chemical,
physical,
methods
preparing
introduced
three
common
enzymes
principles
for
hydrogels.
This
review
properties
prepared
provided
some
suggestions
regarding
current
situation
future
development
cross-linking.
Language: Английский
Highly sensitive amperometric sensors based on laccase-mimetic nanozymes for the detection of dopamine
RSC Advances,
Journal Year:
2024,
Volume and Issue:
14(8), P. 5472 - 5478
Published: Jan. 1, 2024
The
current
research
presents
novel
sensors
based
on
laccase-like
mimetics
for
the
detection
of
dopamine
(DA).
synthesized
nanozymes
(nAuCu,
nPtCu,
nCuMnCo,
and
nCoCuCe)
were
prepared
by
a
simple
hydrothermal
method
exhibited
an
attractive
catalytic
activity
toward
DA.
developed
amperometric
laccase
(nAuCu
nPtCu)
are
more
stable,
selective,
revealed
higher
sensitivity
(6.5-fold
than
biosensor
natural
fungal
from
Trametes
zonata).
obtained
modification
glassy
carbon
electrodes
(GCEs)
with
AuPt
nanoparticles.
Functionalization
electrode
surface
NPs
resulted
in
increased
layer
sensitivity.
Among
studied
configurations,
sensor
containing
nAuCu
nAuPt
possesses
wide
linear
range
(10-170
μM),
lowest
limit
(20
nM),
highest
(10
650
±
8.3
A
M-1
m-2)
at
low
applied
potential
(+0.2
V
versus
Ag/AgCl).
proposed
cost-effective
was
used
determination
DA
pharmaceuticals.
Language: Английский
Biofuel Cells Based on Oxidoreductases and Electroactive Nanomaterials: Development and Characterization
Biosensors,
Journal Year:
2025,
Volume and Issue:
15(4), P. 249 - 249
Published: April 14, 2025
Amperometric
biosensors
(ABSs)
and
enzymatic
biofuel
cells
(BFCs)
share
several
fundamental
principles
in
their
functionality,
despite
serving
different
primary
purposes.
Both
devices
rely
on
biorecognition,
redox
reactions,
electron
transfer
(ET),
advanced
electrode
materials,
including
innovative
nanomaterials
(NMs).
ABSs
BFCs,
utilizing
microbial
oxidoreductases
combination
with
electroactive
NMs,
are
both
efficient
cost-effective.
In
the
current
study,
laboratory
prototypes
of
BFCs
have
been
developed
bioanodes
based
yeast
flavocytochrome
b2
(Fcb2)
alcohol
oxidase
(AO),
a
cathode
fungal
laccase.
For
first
time,
featuring
anodes
Fcb2
co-immobilized
NMs
glassy
carbon
(GCE),
cathode-utilizing
laccase
combined
gold–cerium–platinum
nanoparticles
(nAuCePt).
The
most
effective
lactate
BFC,
which
contains
gold–hexacyanoferrate
(AuHCF),
exhibited
specific
power
density
1.8
µW/cm2.
A
series
were
an
AO-containing
anode
laccase/nAuCePt/GCE
cathode.
optimal
configuration
featured
bioanode
architecture
AO/nCoPtCu/GCE,
achieving
3.2
constructed
tested
using
lactate-containing
food
product
samples
as
fuels.
Language: Английский
Recent Progress on the Rational Design of Laccase Mimics
Jialun He,
No information about this author
Jingtao Li,
No information about this author
Ying Wang
No information about this author
et al.
Chemistry - An Asian Journal,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 21, 2025
Abstract
Laccase,
a
type
of
copper‐containing
natural
oxidase,
is
known
as
green
catalyst
because
only
water
was
produced
the
reduction
product.
It
has
shown
great
potential
for
applications
in
wastewater
treatment,
dye
degradation,
food
and
pharmaceutical
industries,
biosensors,
other
fields.
Despite
above
advantages
laccase,
challenges
arise
from
its
inherent
instability,
recovery
difficulties,
associated
high
costs
exist.
To
address
such
issues,
plethora
nanomaterials
that
possess
laccase‐mimicking
activity,
ranging
monometallic
ions‐containing
to
multimetal‐based
composites,
discovered
past
decade.
In
general,
these
materials
demonstrate
considerable
performance
variability.
A
comprehensive
understanding
design
principles
achieve
laccase‐like
particularly
those
on
Cu
2+
‐involved
structures
related
electron
transfer,
thus
demanded.
Therefore,
this
review,
structure–activity
relationship
native
laccase
first
summarized,
followed
by
categorization
recent
strategies
nanozymes.
After
distilling
insights
currently
reported
nanomaterials,
further
prospect
rational
mimics
with
efficiency
future
also
proposed.
Language: Английский
Emphasizing laccase based amperometric biosensing as an eventual panpharmacon for rapid and effective detection of phenolic compounds
Biochimica et Biophysica Acta (BBA) - General Subjects,
Journal Year:
2024,
Volume and Issue:
1868(11), P. 130691 - 130691
Published: Aug. 6, 2024
Language: Английский
Flavocytochrome b2-Mediated Electroactive Nanoparticles for Developing Amperometric L-Lactate Biosensors
Biosensors,
Journal Year:
2023,
Volume and Issue:
13(6), P. 587 - 587
Published: May 28, 2023
L-Lactate
is
an
indicator
of
food
quality,
so
its
monitoring
essential.
Enzymes
metabolism
are
promising
tools
for
this
aim.
We
describe
here
some
highly
sensitive
biosensors
determination
which
were
developed
using
flavocytochrome
b2
(Fcb2)
as
a
bio-recognition
element,
and
electroactive
nanoparticles
(NPs)
enzyme
immobilization.
The
was
isolated
from
cells
the
thermotolerant
yeast
Ogataea
polymorpha.
possibility
direct
electron
transfer
reduced
form
Fcb2
to
graphite
electrodes
has
been
confirmed,
amplification
electrochemical
communication
between
immobilized
electrode
surface
demonstrated
be
achieved
redox
nanomediators,
both
bound
freely
diffusing.
fabricated
exhibited
high
sensitivity
(up
1436
A·M-1·m-2),
fast
responses,
low
limits
detection.
One
most
effective
biosensors,
contained
co-immobilized
hexacyanoferrate
gold,
having
253
A·M-1·m-2
without
diffusing
mediators,
used
analysis
in
samples
yogurts.
A
correlation
observed
values
analyte
content
determined
biosensor
referenced
enzymatic-chemical
photometric
methods.
based
on
Fcb2-mediated
can
applications
laboratories
control.
Language: Английский
Highly Porous 3D Gold Enhances Sensitivity of Amperometric Biosensors Based on Oxidases and CuCe Nanoparticles
Biosensors,
Journal Year:
2022,
Volume and Issue:
12(7), P. 472 - 472
Published: June 29, 2022
Metallic
nanoparticles
potentially
have
wide
practical
applications
in
various
fields
of
science
and
industry.
In
biosensorics,
they
usually
act
as
catalysts
or
nanozymes
(NZs)
mediators
electron
transfer.
We
describe
here
the
development
amperometric
biosensors
(ABSs)
based
on
purified
oxidases,
synthesized
CuCe
(nCuCe),
micro/nanoporous
gold
(pAu),
which
were
electro-deposited
a
graphite
electrode
(GE).
As
an
effective
peroxidase
(PO)-like
NZ,
nCuCe
was
used
hydrogen-peroxide-sensing
platform
ABSs
that
glucose
oxidase,
alcohol
methylamine
L-arginine
oxidase.
At
same
time,
is
electroactive
mediator
has
been
laccase-based
ABSs.
result,
we
constructed
characterized
glucose,
methanol,
methyl
amine,
L-arginine,
catechol,
respectively.
The
developed
nCuCe-based
exhibited
improved
analytical
characteristics
comparison
with
corresponding
PO-based
Additionally,
presence
pAu,
its
extremely
advanced
chemo-sensing
surface
layer,
shown
to
significantly
increase
sensitivities
all
example,
bioelectrodes
containing
laccase/GE,
laccase/nCuCe/GE,
laccase/nCuCe/pAu/GE
catechol
at
2300,
5055,
9280
A·M
Language: Английский
Laccase mimetics as sensing elements for amperometric assay of 5-hydroxyindoleacetic acid in urine
Bioelectrochemistry,
Journal Year:
2024,
Volume and Issue:
161, P. 108839 - 108839
Published: Oct. 25, 2024
Language: Английский
A new spectrophotometric method analysis of adrenaline in pharmaceuticals based on laccase-like nanozymes
Scientific Messenger of LNU of Veterinary Medicine and Biotechnology,
Journal Year:
2022,
Volume and Issue:
24(106), P. 142 - 148
Published: July 16, 2022
Nanozymes,
which
have
high
enzyme-like
activity
of
natural
enzymes,
are
very
promising
for
analytical
purposes,
in
particular,
the
development
methods
sensitive,
quantitative
detection
practically
important
analytes
–
biomarkers
common
diseases
or
pharmaceutical
products.
Recently,
it
has
been
reported
that
artificial
enzymes
with
laccase-like
“nanolaccases
(nLacs),”
can
serve
as
catalytic
elements
creation
sensitive
catecholamines.
Our
work
aimed
to
obtain
nanozymes
and
characterize
demonstrate
their
suitability
spectrophotometric
adrenaline
(AD)
analysis.
In
this
article,
we
report
on
preparing
five
hexacyanoferrate
nanoparticles
(HCF
NPs)
possess
activity,
particularly,
Co-HCF,
Ni-HCF,
Mn-HCF,
Zn-HCF,
Cu-HCF.
Among
investigated
nLacs,
Cu-HCF
was
selected
characterized.
It
shown
reveals
highest
activities,
is
stable
various
pH
conditions
range
3.0–6.5,
satisfactory
stored
stability.
A
new
method
AD
created
using
nLacs.
The
linearity
proposed
from
5
μM
50
(0.66–11
μg/ml),
limit
1.5
(0.33
lower
than
catalyzed
by
native
laccase
(1.15
μg/ml).
tested
real
samples
pharmaceuticals,
obtained
data
agree
declared
producer.
resulting
nLacs
great
potential
use
catalysis
mimetics,
environmental
restoration,
sensor
design.
Thus
methods,
application
biosensor
analysis
biologically
active
toxic
compounds
surface
waters.
Language: Английский
Highly Sensitive Amperometric Biosensors Based on Oxidases and CuCe Nanoparticles Coupled with Porous Gold
Published: Feb. 14, 2022
Metallic
nanoparticles
are
usually
applied
in
biosensors
as
catalysts
and/or
mediators
of
electron
transfer.
We
describe
the
development
amperometric
(ABSs)
based
on
oxidases
and
CuCe
(nCuCe).
nCuCe,
being
an
electro-active
mediator
active
peroxidase
(PO)
mimetic,
was
used
H2O2-sensing
platform
oxidase-based
ABSs.
ABSs
for
glucose,
primary
alcohols,
methyl
amine,
catechol,
L-arginine,
which
corresponding
were
developed.
These
exhibited
improved
analytical
characteristics
comparison
with
appropriate
bi-enzyme
containing
natural
PO.
Including
electrodeposited
porous
gold
chemo-sensing
layer
shown
to
increase
significantly
sensitivities
all
constructed
Language: Английский