Integration of microfluidic channel on electrochemical-based nanobiosensors for monoplex and multiplex analyses: An overview

Journal of the Taiwan Institute of Chemical Engineers, Journal Year: 2023, Volume and Issue: 146, P. 104814 - 104814

Published: April 6, 2023

Language: Английский

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Deep Learning Enabled Universal Multiplexed Fluorescence Detection for Point-of-Care Applications

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(8), P. 4017 - 4027

Published: July 16, 2024

There is a significant demand for multiplexed fluorescence sensing and detection across range of applications. Yet, the development portable compact multiplexable systems remains substantial challenge. This difficulty largely stems from inherent need spectrum separation, which typically requires sophisticated expensive optical components. Here, we demonstrate compact, lens-free, cost-effective setup that incorporates machine learning scalable detection. method utilizes low-cost components pretrained (ML) model to enable without adjustments. Its multiplexing capability can be easily scaled up through updates altering hardware. We its real-world application in probe-based Loop-Mediated Isothermal Amplification (LAMP) assay designed simultaneously detect three common respiratory viruses within single reaction. The effectiveness this approach highlights system's potential point-of-care applications require solutions. learning-enabled demonstrated work would pave way widespread adoption diverse settings, clinical laboratories field diagnostics.

Language: Английский

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A dual-labeled fluorescence quenching lateral flow assay based on one-pot enzyme-free isothermal cascade amplification for the rapid and sensitive detection of pathogens

Analytical Methods, Journal Year: 2023, Volume and Issue: 15(21), P. 2641 - 2649

Published: Jan. 1, 2023

A dual-labeled fluorescence quenching lateral flow assay based on one-pot, enzyme-free isothermal cascade amplification for the rapid and ultrasensitive detection of pathogens.

Language: Английский

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Nanomaterial-based Electrochemical Sensors for Multiplex Medicinal Applications

Current Topics in Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 24(11), P. 986 - 1009

Published: April 1, 2024

This review explores the advancements in nanomaterial-based electrochemical sensors for multiplex detection of medicinal compounds. The growing demand efficient and selective methods pharmaceutical field has prompted significant research into development employing nanomaterials. These materials, defined as functional materials with at least one dimension between 1 100 nanometers, encompass metal nanoparticles, polymers, carbon-based nanocomposites, nano-bioprobes. are characterized by their enhanced sensitivity selectivity, playing a crucial role simultaneous offering comprehensive analysis multiple complexes within single sample. comprehensively examines design, fabrication, application nanomaterial- based sensors, focusing on ability to achieve various substances. Insights strategies nanomaterials employed enhancing sensor performance discussed. Additionally, challenges future perspectives this evolving field, highlighting potential impact advancement technologies.

Language: Английский

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Candent issues in pneumonia. Reflections from the Fifth Annual Meeting of Spanish Experts 2023

Revista Española de Quimioterapia, Journal Year: 2024, Volume and Issue: 37(3), P. 221 - 251

Published: March 4, 2024

Pneumonia is a multifaceted illness with wide range of clinical manifestations, degree severity and multiple potential causing microorganisms. Despite the intensive research recent decades, community-acquired pneumonia remains third-highest cause mortality in developed countries first due to infections; hospital-acquired main death from nosocomial infection critically ill patients. Guidelines for management this disease are available world wide, but there questions which generate controversy, latest advances make it difficult stay them up date. A multidisciplinary approach can overcome these limitations also aid improve results. Spanish medical societies involved diagnosis treatment have made collaborative effort actualize integrate last expertise about infection. The aim paper reflect knowledge, communicated Fifth Day Spain. It reviews most important disorder, such as microbiological diagnosis, antibiotic sequential therapy, beta-lactam allergic patient, preventive measures, unusual or multi-resistant microorganisms adjuvant advanced therapies Intensive Care Unit.

Language: Английский

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Improving the Accuracy of Single-Nucleotide Variant Diagnosis Using On–Off Discriminating Primers

Biosensors, Journal Year: 2023, Volume and Issue: 13(3), P. 380 - 380

Published: March 13, 2023

Early detection of rare mutations through liquid biopsy can provide real-time information related to cancer diagnosis, prognosis, and treatment outcomes. Cell-free DNA samples used in biopsies contain single-nucleotide variants (SNVs) with a variant allele frequency (VAF) approximately ≤1%. Droplet digital polymerase chain reaction (ddPCR) is considered the gold standard sequencing using samples, generating amplicons from containing 0.001-0.005% VAF; however, it requires expensive equipment time-consuming protocols. Therefore, various PCR methods for discriminating SNVs have been developed; nonetheless, non-specific amplification cannot be avoided even absence mutations, which hampers accurate diagnosis SNVs. In this study, we introduce on-off discrimination-PCR (Soo-PCR), highly practical method that uses 3'-end tailing primer discrimination low-abundance mutant-type targets, including Soo-PCR minimizes chance incorrect judgments owing its high power. Cancer markers, such as KRAS G12D, EGFR L858R, T790M 0.1% VAF, were clearly detected under 2 h reliability comparable ddPCR. This new serves approach accurately detect evaluate user-friendly manner.

Language: Английский

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Understanding COVID-19 for future applications in pathogen detection: groundbreaking technology via the Arduino-driven RT-LAMP method

Published: Jan. 29, 2024

Timely and effective detection of COVID-19 continues to be a critical aspect in managing the pandemic setting groundwork for future pathogen techniques. Present diagnostic technologies, especially those designed POC (Point Care) use resource-constrained settings, must meet evolving needs rapidity, accuracy, affordability. Within this context, preparing pandemics necessitates development swift diagnostics. This research investigates potential Arduino-driven LAMP (Loop-Mediated Isothermal Amplification) method SARS-CoV-2 detection. An inexpensive, accessible, portable "LAMP box" is proposed study, integrating assay with an Arduino create potentially system COVID-19. The device features wireless technology network connecting all components. reaction performed at 65°C on isothermal heating pad located beneath solid aluminum base, providing stable platform tubes assays. Control entire system, powered via 5-volt USB source, provided through built-in Bluetooth connection linked Arduino, facilitating control computer. study concludes that such simple systems can effectively determine presence or absence artificial genetic material samples. presented underscores promise Arduino-based technology, showcasing its specificity sensitivity compared conventional RT-PCR (Reverse Transcription Polymerase Chain Reaction).

Language: Английский

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Revista Española de Quimioterapia, Journal Year: 2024, Volume and Issue: 37(3)

Published: May 10, 2024

Tipos de estudios clínicos y pautas para su correcta interpretación post-pandemia RESUMENLos ensayos aleatorizados (ECA) son claves en el avance la medicina microbiología, pero no única opción posible.Los observacionales proporcionan información sobre eficacia seguridad a largo plazo, menos costosos, permiten estudiar eventos poco frecuentes obtener más rápido que los ECA.En contra, vulnerables factores confusión.Los prospectivos piloto exploratorios, comparten con ECA muchos aspectos, están sujetos supervisión por parte comisiones externas ni obligatoriedad registro.El multitesting, puede pervertir balance publicaciones favor del efecto buscado.El razonamiento Bonferroni muestra que, si se realizan 10 un antibiótico efectivo, probabilidad al uno arroje P <0,05 pude ser 40%.Escenarios hay mucha presión investigar, como reciente pandemia, propicios equipos traten antimicrobiano.Aunque fármaco tenga ninguna eficacia, 100 investigación estudio valorar utilidad, prácticamente seguro aparecerá <0,05.Si demás (con >0,05) publican, comunidad científica consideraría fuerte evidencia utilidad.En conclusión, una muy buena fuente clínica, única.El registro sistemático todas las investigaciones iniciadas debe aplicarse todo tipo clínicos.

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Electrochemical Sensors and Biosensors for Identification of Viruses: A Critical Review

Critical Reviews in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 30

Published: May 16, 2024

Due to their life cycle, viruses can disrupt the metabolism of hosts, causing diseases. If we want it is necessary identify presence. For this purpose, possible use several molecular-biological and bioanalytical methods. The reference selection was performed based on electronic databases (2020-2023). This review focused electrochemical methods with high sensitivity selectivity (53% voltammetry/amperometry, 33% impedance, 12% other methods) which showed great potential for detecting various viruses. Moreover, aforementioned have considerable be applicable care-point as they are portable due miniaturizability fast speed analysis (minutes hours), relatively easy interpret. A total 2011 articles were found, 86 original papers subsequently evaluated (the majority human pathogens, whereas dealing plant pathogens in minority). Thirty-two species included evaluation. It found that most examined research studies (77%) used nanotechnological modifications. Other ones immunological (52%) or genetic analyses (43%) virus detection. 5% reports peptides increase method's sensitivity. When evaluable, 65% had LOD values order ng nM. vast (79%) represent proof concept possibilities low application a need further experimental work.

Language: Английский

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Advances in Amplification Methods for Biosensors

Biosensors, Journal Year: 2023, Volume and Issue: 13(3), P. 365 - 365

Published: March 10, 2023

Today, there is a rapidly growing demand for sensitive and selective biosensors in various domains, including environmental monitoring such as (waste)water control, detection of pollution personal/public safety, agricultural/food safety quality veterinary medical diagnostics, etc [...]

Language: Английский

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