Controlling Fluorescent Readout in Paper-based Analytical Devices DOI Creative Commons
Anna Luongo,

Alexander Ritter von Stockert,

Frank D. Scherag

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2023, Volume and Issue: 9(11), P. 6379 - 6389

Published: Oct. 24, 2023

Paper is an ideal candidate for the development of new disposable diagnostic devices because it a low-cost material, allows transport liquid on device by capillary action, and environmentally friendly. Today, colorimetric analysis most often used as detection method rapid tests (test strips or lateral flow devices) but usually gives only qualitative results limited relatively high threshold. Here, we describe studies using fluorescence readout tool paper-based diagnostics. We study how optical affected light transmission, scattering, function paper characteristics such thickness (grammage), water content, autofluorescence, type/composition. show that better compared to nitrocellulose, which currently material choice in assays. To reduce loss analyte molecules (e.g., proteins) due adsorption surface, coat fibers with protein-repellent hydrogel. For this purpose, use hydrophilic copolymers consisting N,N-dimethyl acrylamide benzophenone-based cross-linker, are photochemically transformed into fiber-attached polymer hydrogel fiber surfaces situ. combination coating enables sensitive analysis. Finally, success strategy demonstrated simple LFD application example.

Language: Английский

Microfluidic-assisted integrated nucleic acid test strips for POCT DOI
Nan Wang, Juan Zhang, Bin Xiao

et al.

Talanta, Journal Year: 2023, Volume and Issue: 267, P. 125150 - 125150

Published: Sept. 3, 2023

Language: Английский

Citations

26
Catalytic hairpin self-assembly for biosensing: Classification, influencing factors, and applications DOI
Luyang Zhao, Yang Song, Hengyi Xu

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 171, P. 117508 - 117508

Published: Dec. 28, 2023

Language: Английский

Citations

19
Revolutionizing Cervical Cancer Diagnostics: A Shift from Traditional Techniques to Biosensors DOI Creative Commons

Ubaid Mushtaq Naikoo,

Roberto Pilloton, Humaira Farooqi

et al.

Biosensors and Bioelectronics X, Journal Year: 2025, Volume and Issue: unknown, P. 100587 - 100587

Published: Jan. 1, 2025

Language: Английский

Citations

0
Novel Multimode Assay Based on Asymmetrically Competitive CRISPR and Raman Barcode Spectra for Multiple Hepatocellular Carcinoma Biomarkers Detection DOI
Hao Jiang, Cheng Qian, Yulin Deng

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Commercial pregnancy test strips (PTS) possess the advantages of lower price, higher stability, and better repeatability have been popularized to integrate with novel sensing strategies detect other disease biomarkers, which accelerates commercialization process those strategies. However, current integration into commercial PTS still faced problems insufficient quantification, low sensitivity, lack multiple detection capabilities. Hence, we proposed concept "visual classification recognition, spectral signal subdivision" for hepatocellular carcinoma biomarkers (miRNA122 miRNA233) dual signals based on asymmetric competitive CRISPR (acCRISPR) surface-enhanced Raman spectroscopy coupling PTS, named acCRISPR-PTS-SERS assay. In this assay, acCRISPR was used as a nonamplified cascaded amplification method improve sensitivity detection. Two AuNPs-based core-shell tags, each corresponding different miRNA were achieve both visual recognition segmentation enhance quantification capability Under optimal conditions, LOD miRNA122 miRNA223 10.36 4.65 fM, respectively. The enhanced by nearly 2 orders magnitude. future, simultaneous hand-held fingerprint barcodes cancers can be achieved assistance microfluidic chip smartphone.

Language: Английский

Citations

3
Microfluidic chip immunoassay based on rolling circle amplification and G-quadruplex/Thioflavin T for multiplex detection of CTX I DOI
Hao Jiang,

Xuefei Lv,

Shuyu Lu

et al.

Microchimica Acta, Journal Year: 2024, Volume and Issue: 191(3)

Published: Feb. 28, 2024

Language: Английский

Citations

2
Advancements and future directions in cardiac biomarker detection using lateral flow assays DOI
Stella Schuster da Silva,

Mylena Lemes Cunha,

Lucas B. Ayres

et al.

Analytical Methods, Journal Year: 2023, Volume and Issue: 15(30), P. 3610 - 3630

Published: Jan. 1, 2023

Lateral flow assays (LFAs) have emerged as one of the most prominent paper-based biosensor platforms for rapidly detecting and quantifying analytes linked to cardiovascular health.

Language: Английский

Citations

5
Controlling Fluorescent Readout in Paper-based Analytical Devices DOI Creative Commons
Anna Luongo,

Alexander Ritter von Stockert,

Frank D. Scherag

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2023, Volume and Issue: 9(11), P. 6379 - 6389

Published: Oct. 24, 2023

Paper is an ideal candidate for the development of new disposable diagnostic devices because it a low-cost material, allows transport liquid on device by capillary action, and environmentally friendly. Today, colorimetric analysis most often used as detection method rapid tests (test strips or lateral flow devices) but usually gives only qualitative results limited relatively high threshold. Here, we describe studies using fluorescence readout tool paper-based diagnostics. We study how optical affected light transmission, scattering, function paper characteristics such thickness (grammage), water content, autofluorescence, type/composition. show that better compared to nitrocellulose, which currently material choice in assays. To reduce loss analyte molecules (e.g., proteins) due adsorption surface, coat fibers with protein-repellent hydrogel. For this purpose, use hydrophilic copolymers consisting N,N-dimethyl acrylamide benzophenone-based cross-linker, are photochemically transformed into fiber-attached polymer hydrogel fiber surfaces situ. combination coating enables sensitive analysis. Finally, success strategy demonstrated simple LFD application example.

Language: Английский

Citations

5