Label-free optical biosensing: going beyond the limits

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(18), P. 6554 - 6585

Published: Jan. 1, 2023

Label-free optical biosensing holds great promise for a variety of applications in biomedical diagnostics, environmental and food safety, security. It is already used as key tool the investigation biomolecular binding events reaction constants real time offers further potential additional functionalities low-cost designs. However, sensitivity this technology does not match routinely but expensive slow labelling methods. Therefore, label-free remains predominantly research tool. Here we discuss how one can go beyond limits detection provided by standard platforms achieve that superior to To end review newly emerging implementations overcome current barriers employing novel structural architectures, artificial materials (metamaterials hetero-metastructures) using phase light sensing parameter. Furthermore, elucidate mechanism plasmonic hyper-sensitive transducers, which at single molecule level (less than 1 fg mm-2) make it possible detect analytes several orders magnitude lower concentrations so far reported literature. We finally layouts based on dielectric nanomaterials, bound states continuum, exceptional points.

Language: Английский

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Metasurface Biosensors Enabling Single-Molecule Sensing of Cell-Free DNA

Nano Letters, Journal Year: 2023, Volume and Issue: 23(12), P. 5755 - 5761

Published: June 14, 2023

In this study, we have revealed that highly fluorescence (FL)-enhancing all-dielectric metasurface biosensors are capable of detecting single-target DNA, which is cell-free DNA (cfDNA) specific to the human practice effect. The ultimately high-precision detection was achieved in a scheme combining with short-time nucleic acid amplification technique, is, reduced-cycle polymerase chain reaction (PCR). combined scheme, obtained series FL signals at single-molecule concentration, reflecting Poisson distribution, and moreover elucidated exhibit cfDNA more than 84% statistical confidence an automated system 99.9% confocal microscopy. As result, found simple practical test discriminate target 1 copy/test from zero using biosensors, has not been realized by other elaborate techniques such as digital PCR.

Language: Английский

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Productive biosensing techniques empowered by all-dielectric metasurfaces

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 9, 2025

Artificially designed, functional nanostructured surfaces, called metasurfaces, are an emerging platform for biosensing. Two major types of metasurface biosensors have been reported: one is based on resonant-wavelength shift and the other specialized fluorescence (FL) detection. The all-dielectric metasurfaces that composed periodic arrays silicon nanocolumns a series optical magnetic-mode resonances, some which were found to significantly enhance capability FL detection diverse target biomolecules, ranging from nucleic acid antigens antibodies. Here, we mainly address recent advances in productive biosensors, provide overview pivotal results, discuss future prospects, including artificial-intelligence-driven big data analysis next-generation healthcare services.

Language: Английский

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Metasurface-enhanced biomedical spectroscopy

Nanophotonics, Journal Year: 2025, Volume and Issue: 14(8), P. 1045 - 1068

Published: Jan. 17, 2025

Abstract Enhancing the sensitivity of biomedical spectroscopy is crucial for advancing medical research and diagnostics. Metasurfaces have emerged as powerful platforms enhancing various spectral detection technologies. This capability arises from their unparalleled ability to improve interactions between light matter through localization enhancement fields. In this article, we review representative approaches recent advances in metasurface-enhanced spectroscopy. We provide a comprehensive discussion technologies enhanced by metasurfaces, including infrared spectroscopy, Raman fluorescence other modalities. demonstrate advantages metasurfaces improving sensitivity, reducing limits, achieving rapid biomolecule while discussing challenges associated with design, preparation, stability procedures. Finally, explore future development trends biological emphasize wide-ranging applications.

Language: Английский

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Metasurface Platform Incorporating Aggregation Induced Emission Based Biosensor for Enhanced Human Serum Albumin Detection

Advanced Optical Materials, Journal Year: 2024, Volume and Issue: 12(23)

Published: June 4, 2024

Abstract Metasurfaces exhibit excellent optical performance to enhance the light–matter interaction of target molecules in biosensing based on its well‐optimized nanostructured unit cells. In meantime, fluorescence (FL) biosensors with aggregation induced emissions (AIE) features also demonstrate outstanding biomarker detection due their fast response, high selectivity, and low background noise. Nevertheless, extremely low‐level analytes are difficult detect practical applications since complex urine samples include a number uncontrolled variables such as impurities, autofluorescence, other components, etc. At present, improving signal sensitivity human serum albumin (HSA) is always big challenge overcome interference scenarios. this work, first an AIE‐based FL biosensor TPE‐4TA combined all‐dielectric metasurface platform achieve quantitative trace HSA by utilizing biofunctionalization protocols silicon (Si) nanostructures. The results indicate significant enhancement that offers promising pathway for future.

Language: Английский

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Robust Detection of Cancer Markers in Human Serums Using All-Dielectric Metasurface Biosensors

Biosensors, Journal Year: 2023, Volume and Issue: 13(3), P. 377 - 377

Published: March 13, 2023

One of the most significant characteristics, which biosensors are supposed to satisfy, is robustness against abundant molecules coexisting with target biomolecules. In clinical diagnoses and biosensing, blood, plasma, serum used daily as samples. this study, we conducted a series experiments examine all-dielectric metasurface biosensors, comprise pairs highly fluorescence-enhancing silicon nanopellet array transparent microfluidic chip. The were shown have high performance in detecting various targets from nucleic acids proteins, such antigens antibodies. present results show almost four-order wide dynamic ranges 0.16 ng/mL 1 μg/mL for prostate-specific antigen (PSA) 2 pg/mL 25 carcinoembryonic (CEA). include criteria PSA, 4 CEA, 5 ng/mL. To date, systematic demonstration has not been reported regarding biosensors. cancer markers PSA CEA human serums, demonstrate that robust enough concentrations, including diagnosis criteria.

Language: Английский

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Metasurface biosensors: Status and prospects

Applied Physics Reviews, Journal Year: 2025, Volume and Issue: 12(2)

Published: April 4, 2025

Metasurfaces have emerged as a rapidly evolving frontier in the fields of optics and photonics, with growing emphasis on their potential for practical applications. The considerable volume contributions to study metasurfaces has expanded, creating challenges tracking all advancements within this dynamic field. In review, we select practically useful among diverse studied so far. We refer selected hot research topics at beginning, succeedingly outline status several applications that are nearing applications, then focus biosensing particular attention metasurface fluorescence (FL) biosensors, because FL detection is major approach bioscience biotechnology. However, by biosensors not been reviewed an extensive comprehensive manner. Indeed, demonstrated capability detecting wide range biomolecules including nucleic acids proteins, such antigens antibodies. Notably, they offer enhanced sensitivity assays reduced assay time when compared conventional commercial assays. here provide focused review current future directions biosensors.

Language: Английский

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2D‐Material‐Fused High‐Emittance Plasmo–Photonic Metasurfaces

Advanced Optical Materials, Journal Year: 2024, Volume and Issue: 12(17)

Published: April 3, 2024

Abstract Transition metal dichalcogenides (TMDC) are a family of atomic‐layer 2D materials (2dMs). Many researches have attempted to reinforce the unique properties by incorporating artificially designed nanostructures. Here, highly photoluminescence (PL) enhancing platform is reported that formed combining high‐emittance plasmo–photonic metasurfaces with large‐area 2dM, which continuous monolayer TMDC cm 2 dimension. It experimentally demonstrated 2dM‐PL intensity at room temperature enhanced more than 1030‐fold in comparison observed on flat gold film regions. The enhancement associated spectral transformation indicating resonant effect metasurfaces. moreover revealed confocal PL image originates from coherent single‐mode emission forms photon number distribution obeying Poisson probability distribution. Thus, 2dM‐fused function as efficient quantum light sources. decay‐time analysis also reveals coexistence two components PL. fast component exhibits lifetime 18 ps whereas slow decays 4.4 ns. Both most likely undergo radiative processes, indicates metal‐induced quenching suppressed these long ascribed an excitonic intervalley transition.

Language: Английский

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Subwavelength periodic dielectric nanostructures for biochemical sensing

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 157 - 187

Published: Jan. 1, 2024

Language: Английский

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Prominently enhanced luminescence from a continuous monolayer of transition metal dichalcogenide on all-dielectric metasurfaces

Nanophotonics, Journal Year: 2023, Volume and Issue: 13(1), P. 95 - 105

Published: Dec. 25, 2023

Abstract 2D materials such as transition metal dichalcogenides (TMDCs) are a new class of atomic-layer possessing optical and electric properties that significantly depend on the number layers. Electronic transitions can be manipulated in artificial resonant electromagnetic (EM) fields using metasurfaces other designed nanostructures. Here, we demonstrate prominently enhancement photoluminescence (PL) atomic monolayer, WS 2 , doped with small quantity Mo. The excitonic PL showed strong effect higher-order magnetic resonance all-dielectric consisting periodic arrays Si nanopellets. intensity witnessed 300-fold compared to reference flat silicon dioxide (SiO ) layer, which suggests drastic change dynamics photoexcited states. Confocal microscopy analysis revealed single photons were coherently emitted from TMDC monolayer metasurface. Furthermore, examining lifetime ps ns timescales clarified two exponential components at prominent exciton PL: short-time component decaying 22 long-time lasting over 10 ns. Therefore, infer radiative activated comparison SiO layer.

Language: Английский

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