Journal of Translational Medicine,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: Dec. 2, 2024
To
investigate
the
clinical
application
value
of
microfluidic-based
in
vitro
diagnostic
(IVD)
technology
pathogenic
detection
respiratory
tract
infections.
A
total
300
samples,
including
blood,
bronchoalveolar
lavage
fluid,
and
pleural
effusion,
were
collected
from
patients
with
The
samples
randomly
divided
into
three
groups:
A,
B,
C,
100
cases
each
group.
Group
used
traditional
microbiological
methods,
B
metagenomic
next-generation
sequencing
(mNGS)
technology,
C
both
IVD
methods
to
detect
microorganisms
samples.
positive
rate,
time,
cost
compared
among
groups.
performance
group
was
using
Receiver
Operating
Characteristic
(ROC)
curve.
Traditional
identified
38
(38%),
45
pathogens;
mNGS
95
(95%),
210
96
(96%),
158
pathogens.
Microfluidic-based
had
a
significantly
higher
rate
for
culture
techniques
(96%
vs
38%,
χ2
=
122.0,
P
<
0.01),
it
also
faster
cheaper
than
technology.
ROC
analysis
showed
that
results,
increased
sensitivity
specificity,
similar
In
infectious
diseases,
advantages
time
It
could
critical
drug-resistant
genes
Hence,
can
be
viable
option
diagnosis
treatment
diseases.
International Journal on Smart Sensing and Intelligent Systems,
Journal Year:
2025,
Volume and Issue:
18(1)
Published: Jan. 1, 2025
Abstract
In
recent
years,
the
point-of-care
testing
(POCT)
device
for
nucleic
acid
detection
has
received
extensive
attention.
POCT
provides
rapid,
high
sensitivity
and
accuracy
of
on-site
diagnosis
can
quantify
in
clinical
samples
real
time.
The
integration
microfluidics
ensures
accurate
fluid
control,
minimizes
sample
volume,
improves
efficiency.
addition,
devices
conduct
multichannel
analysis,
facilitate
continuous
monitoring,
provide
comprehensive
quantitative
data.
Due
to
its
portability,
cost-effectiveness,
ease
use,
represents
a
promising
solution
extensive,
reliable,
timely
various
medical
environments.
This
review
summarizes
research
development
based
on
microfluidic
technology
trend
prospected.
Analytical Methods,
Journal Year:
2024,
Volume and Issue:
16(7), P. 971 - 978
Published: Jan. 1, 2024
Virus-based
human
infectious
diseases
have
a
significant
negative
impact
on
people's
health
and
social
development.
The
need
for
quick,
accurate,
early
viral
infection
detection
in
preventive
medicine
is
expanding.
A
microfluidic
control
particularly
suitable
point-of-care-testing
virus
diagnosis
due
to
its
advantages
of
low
sample
consumption,
quick
speed,
simple
operation,
multi-functional
integration,
small
size,
easy
portability.
It
also
thought
development
potential
wide
range
application
prospects
the
research
technology.
In
an
effort
aid
researchers
creating
novel
tools
detection,
this
review
highlights
recent
developments
droplet-based
microfluidics
discusses
challenges
opportunities
rapid
detection.
Biosensors,
Journal Year:
2024,
Volume and Issue:
14(11), P. 545 - 545
Published: Nov. 10, 2024
Rheumatoid
arthritis
(RA)
is
a
chronic
autoimmune
disorder
that
causes
extensive
damage
to
multiple
organs
and
tissues
has
no
known
cure.
This
study
introduces
microfluidic
detection
platform
combines
reaction
chip
with
micro-spectrometer
accurately
detect
the
anti-cyclic
citrullinated
peptide
antibody
(anti-CCP
Ab)
biomarker,
commonly
associated
arthritis.
The
surface
of
functionalized
using
streptavidin
enable
subsequent
immobilization
biotinylated-labeled
cyclic
(biotin-CCP)
molecules
through
streptavidin-biotin
reaction.
modified
then
exposed
anti-CCP
Ab,
second
conjugated
horseradish
peroxidase
(HRP)
(2nd
Ab-HRP),
3,3',5,5'-tetramethylbenzidine
(TMB),
stop
solution.
Finally,
concentration
Ab
biomarker
determined
by
analyzing
optical
density
(OD)
colorimetric
product
at
450
nm
micro-spectrometer.
demonstrated
strong
correlation
(R
Environmental Science Water Research & Technology,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 1, 2024
Integrating
point-of-care
diagnostics
into
SARS-CoV-2
wastewater
surveillance
enables
rapid
detection
and
response.
This
innovation
enhances
pandemic
management
has
potential
for
broader
public
health
applications
beyond
COVID-19.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(15), P. 8282 - 8282
Published: July 29, 2024
The
rapid
and
accurate
diagnosis
of
meningitis
is
critical
for
preventing
severe
complications
fatalities.
This
study
addresses
the
need
accessible
diagnostics
in
absence
specialized
equipment
by
developing
a
novel
diagnostic
assay.
assay
utilizes
dual-priming
isothermal
amplification
(DAMP)
with
unique
internal
primers
to
significantly
reduce
non-specificity.
For
fluorescence
detection,
dye
was
selected
among
Brilliant
Green,
Thioflavin
T,
dsGreen.
Green
preferred
this
due
its
availability,
high
level,
optimal
sample-to-background
(S/B)
ratio.
developed
detection
primary
causative
agents
(Haemophilus
influenzae,
Neisseria
meningitidis,
Streptococcus
pneumoniae),
tested
on
clinical
samples.
method
demonstrated
specificity,
no
false
positives,
sensitivity
comparable
that
loop-mediated
(LAMP),
S/B
versatile
can
be
utilized
as
standalone
test
or
an
integrated
into
point-of-care
systems
reliable
pathogen
detection.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2024,
Volume and Issue:
12
Published: Aug. 21, 2024
Introduction
This
systematic
review
and
meta-analysis
present
a
comprehensive
evaluation
of
paper-based
microfluidic
devices,
focusing
on
their
applications
in
immunoassays.
These
devices
are
emerging
as
innovative
solutions
to
democratize
access
diagnostic
technologies,
especially
resource-limited
settings.
Our
consolidates
findings
from
diverse
studies
outline
advancements
technology,
including
design
intricacies
operational
efficacy.
Key
advantages
such
low
cost,
portability,
ease
use
highlighted.
Materials
Methods
The
categorizes
literature
based
the
nuances
these
tools,
exploring
various
methodologies,
fabrication
techniques,
detection
methods,
applications,
particularly
protein
science.
extends
providing
framework
for
classifying
stratifying
uses
diagnostics.
Results
discussion
Notable
include
critical
analysis
performance
metrics,
sensitivity
specificity.
addresses
challenges,
need
further
validation
optimization
broader
clinical
applications.
A
processes,
cross-validation
rigorous
control
testing,
is
provided
ensure
robustness
devices.
study
offers
novel
insights
into
computational
strategies
underpinning
technologies
serves
roadmap
future
research,
potentially
broadening
impact
across
science
universe.
