Nanoscale, Journal Year: 2024, Volume and Issue: 16(29), P. 13802 - 13819
Published: Jan. 1, 2024
The detection and identification of microorganisms are crucial in microbiology laboratories.
Language: Английский
Journal of The Electrochemical Society, Journal Year: 2024, Volume and Issue: 171(8), P. 087502 - 087502
Published: July 20, 2024
Zoonotic diseases are a pressing challenge to global health, arising from their interspecies transmission and potential for pandemics. Conventional diagnostic methodologies often suffer limitations in speed, sensitivity, accuracy, underscoring the imperative innovative solutions. Nanomaterial-integrated biosensors, especially electrochemical modules, have gained increasing attention as promising tools rapid, sensitive, point-of-care targeted detection of zoonotic pathogens. Despite considerable progress, pervasive challenges, including market fragmentation research disparities, impede widespread adoption. This review explores essential role biosensors combating diseases, urgency accessible, cost-effective, high-throughput platforms. It details principles biosensing highlights diversified nanomaterials enhancing performance biosensors. Furthermore, it examines integration with different electrodes signal amplification strategies improve its sensing performance. current alternate solutions perspectives developing translating these diagnosis/surveillance diseases. provides valuable insights into nanomaterial-based revolutionize diagnosis management ultimately contributing efforts mitigate impact contagious threats on human animal health.
Language: Английский
Citations
10
Trends in Food Science & Technology, Journal Year: 2025, Volume and Issue: unknown, P. 104882 - 104882
Published: Jan. 1, 2025
Language: Английский
Citations
1
Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 242, P. 115742 - 115742
Published: Oct. 5, 2023
Language: Английский
Citations
15
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 21, 2025
Streptococcus suis, a significant zoonotic pathogen, annually caused substantial economic losses in the swine industry and had intensified threat to public health due recent emergence of human-associated clade. In this study, we discovered that rare-earth metal-based metal-organic frameworks (Y-BTC) possessed excellent ECL capabilities. After prereduction at high voltage, its intensity was enhanced by two times. Subsequently, developed an efficient CRISPR/Cas12a-mediated electrochemiluminescence resonance energy transfer (ECL-RET) biosensor utilizing Y-BTC for detection S. suis employed as ECL-RET donor emitter, spherical nucleic acid Au NP utilized receptor. presence target, isothermal amplification triggered generate large number amplicons, which subsequently activated trans-cleavage activity Cas12a. Cas12a cleaved shell on surface NPs, reducing spatial distance between NPs electrostatic adsorption, thereby quenching via ECL-RET. Consequently, targets can be observed reduced signal. The sensor exhibited range 25 pM 50 nM, with limit low 17 pM. practical utility verified through actual sample testing. Our proposed sensing strategy provides new avenue sensitive suis. universality has also been demonstrated using Fusobacterium nucleatum, Salmonella pullorum, Listeria monocytogenes, holding great promise field food safety health.
Language: Английский
Citations
0
Phenomics, Journal Year: 2025, Volume and Issue: unknown
Published: March 4, 2025
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: March 26, 2025
Language: Английский
Citations
0
Discover Nano, Journal Year: 2025, Volume and Issue: 20(1)
Published: April 2, 2025
This paper provides a comprehensive overview of the diverse applications and innovations nanoparticles in detection Salmonella. It encompasses range novel methods, including efficient enrichment, nucleic acid extraction, immunoassays, tests, biosensors, emerging strategies with potential for future applications. The surface modification specific antibodies or ligands enables to achieve highly selective capture Salmonella, while optimizing extraction process improving efficiency. employment immunological tests markedly enhances specificity sensitivity reaction, thereby determination results. Moreover, distinctive physicochemical properties enhance sensitivity, selectivity, stability facilitating rapid advancement bio-detection technologies. is particularly noteworthy that there has been significant application innovative research nanozymes molecular assays. progress not only resulted enhanced efficiency but also facilitated innovation improvement As nanotechnologies continue advance, use metal oxide Salmonella likely become more promising reliable strategy ensuring food safety public health.
Language: Английский
Citations
0
Foods, Journal Year: 2024, Volume and Issue: 13(19), P. 3032 - 3032
Published: Sept. 24, 2024
Salmonella typhimurium and Listeria monocytogenes are relevant foodborne bacterial pathogens which may cause serious intoxications infectious diseases in humans. In this study, a sensitive immunochromatographic analysis (ICA) for the simultaneous detection of these two was developed. For this, test strips containing zones with specific monoclonal antibodies (MAb) against lipopolysaccharides S. L. one control zone secondary were designed, double-assay conditions optimized to ensure high analytical parameters. Prussian blue nanoparticles (PBNPs) used as nanozyme labels conjugated MAbs perform sandwich format ICA. Peroxidase-mimic properties PBNPs allowed catalytic amplification colorimetric signal on strips, enhancing assay sensitivity. The limits (LODs) cells 2 × 102 7 103 cells/mL, respectively. LODs 100-fold less than those achieved due ICA based traditional gold label. developed double approbated bacteria cow milk samples, processed by simple dilution buffer before assay. monocytogenes, recoveries from 86.3 ± 9.8 118.2 10.5% correlated well estimated enzyme-linked immunosorbent reference method. proposed approach characterized specificity: no cross-reactivity other strains observed. satisfies requirements rapid tests: full cycle sample acquisition result assessment half an hour. has application potential multiplex pathogens.
Language: Английский
Citations
2
Biosensors, Journal Year: 2024, Volume and Issue: 14(6), P. 292 - 292
Published: June 4, 2024
Olaquindox (OLA) and quinocetone (QCT) have been prohibited in aquatic products due to their significant toxicity side effects. In this study, rapid visual europium nanoparticle (EuNP)-based lateral flow strip biosensors (LFSBs) were developed for the simultaneous quantitative detection of OLA, QCT, 3-methyl-quinoxaline-2-carboxylic acid (MQCA) fish feed tissue. The EuNP-LFSBs enabled sensitive MQCA with a limit 0.067, 0.017, 0.099 ng/mL (R2 ≥ 0.9776) within 10 min. average recovery was 95.13%, relative standard deviations below 9.38%. method verified by high-performance liquid chromatography (HPLC), test results consistent. Therefore, proposed LFSBs serve as powerful tool monitor quinoxalines feeds residues tissues.
Language: Английский
Citations
0
Nanoscale, Journal Year: 2024, Volume and Issue: 16(29), P. 13802 - 13819
Published: Jan. 1, 2024
The detection and identification of microorganisms are crucial in microbiology laboratories.
Language: Английский
Citations
0