Split Probe-Induced Protein Translational Amplification for Nucleic Acid Detection DOI
Yoo Hong Min,

Yoonseo Hong,

Cheol‐Hee Kim

et al.

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

Nucleic acid detection is important in a wide range of applications, including disease diagnosis, genetic testing, biotechnological research, environmental monitoring, and forensic science. However, the application nucleic various fields hindered by lack sensitive, accurate, inexpensive methods. This study introduces simple approach to enhance sensitivity for accurate acids. Our combined split-probe strategy with vitro translational amplification reporter protein signal generation detect acids high selectivity. enables target-mediated proteins linking split probes presence target microRNA (miRNA). In particular, fluorescence sensor adopts wavelength regions, enabling simultaneous multiple miRNAs. Moreover, luminescence merely altering sequence can substantially Using this system, we analyzed quantified miRNAs total RNA extracted from cell lines cell-derived extracellular vesicles specificity accuracy. has potential as powerful tool simple, specific

Language: Английский

CRISPR-Driven Biosensors: A New Frontier in Rapid and Accurate Disease Detection DOI

ZahraSadat Razavi,

M. Soltani, Mohammad Souri

et al.

Critical Reviews in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 25

Published: Sept. 17, 2024

This comprehensive review delves into the advancements and challenges in biosensing, with a strong emphasis on transformative potential of CRISPR technology for early rapid detection infectious diseases. It underscores versatility CRISPR/Cas systems, highlighting their ability to detect both nucleic acids non-nucleic acid targets, seamless integration isothermal amplification techniques. The provides thorough examination latest developments CRISPR-based biosensors, detailing unique properties such as high specificity programmability, which make them particularly effective detecting disease-associated acids. While focuses due its critical role diagnosing diseases, it also explores broader applications thereby acknowledging technology's potential. Additionally, identifies existing challenges, need improved signal real-world applicability, offers future perspectives aimed at overcoming these hurdles. ultimate goal is advance development highly sensitive specific biosensors that can be used widely improving human health, point-of-care settings resource-limited environments.

Language: Английский

Citations

14
Simultaneous detection of dengue virus serotypes in a dual-serotype-detection nucleic acid based lateral flow assay DOI
Biswadeep Das, Sagnika Samal, Hamida Hamdi

et al.

Diagnostic Microbiology and Infectious Disease, Journal Year: 2025, Volume and Issue: 111(3), P. 116679 - 116679

Published: Jan. 6, 2025

Language: Английский

Citations

1
Point-of-care isothermal nucleic acid amplification tests: progress and bottlenecks for extraction-free sample collection and preparation DOI
Alexis F. Wilkinson, Maria Barra, Emilie Newsham Novak

et al.

Expert Review of Molecular Diagnostics, Journal Year: 2024, Volume and Issue: 24(6), P. 509 - 524

Published: June 2, 2024

Introduction Suitable sample collection and preparation methods are essential to enable nucleic acid amplification testing at the point of care (POC). Strategies that allow direct isothermal (iNAAT) crude lysate without need for extraction minimize time result as well operator expertise costly infrastructure.

Language: Английский

Citations

5
Phage-Derived Endolysins Against Resistant Staphylococcus spp.: A Review of Features, Antibacterial Activities, and Recent Applications DOI Creative Commons

Mina Golban,

Javad Charostad,

Hossein Kazemian

et al.

Infectious Diseases and Therapy, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 16, 2024

Antimicrobial resistance is a significant global public health issue, and the dissemination of antibiotic in Gram-positive bacterial pathogens has significantly increased morbidity, mortality rates, healthcare costs. Among them, Staphylococcus, especially methicillin-resistant Staphylococcus aureus (MRSA), causes wide range diseases due to its diverse pathogenic factors infection strategies. These bacteria also present issues veterinary medicine food safety. Effectively managing staphylococci-related problems necessitates concerted effort implement preventive measures, rapidly detect pathogen, develop new safe antimicrobial therapies. In recent years, there been growing interest using endolysins combat infections. enzymes, which are referred as lysins, unique class hydrolytic enzymes synthesized by double-stranded DNA bacteriophages. They possess glycosidase, lytic transglycosylase, amidase, endopeptidase activities, effectively destroying peptidoglycan layer resulting lysis. This property makes powerful agents, particularly against organisms with more accessible layers. Therefore, considering potential benefits compared conventional antibiotics, we have endeavored gather review characteristics uses derived from staphylococcal bacteriophages, well their antibacterial effectiveness spp. based on conducted experiments trials.

Language: Английский

Citations

4
A Novel Rapid Detection Method for Mycobacterium tuberculosis Based on Scattering-Light Turbidity Using Loop-Mediated Isothermal Amplification DOI Creative Commons
Meimei Zeng, Xinru Wang,

Zifeng Tan

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(3), P. 162 - 162

Published: March 3, 2025

The accurate detection of Mycobacterium tuberculosis (MTB) is a pressing challenge in the precise prevention and control tuberculosis. Currently, efficiency accuracy drug resistance for MTB are low, cross-contamination common, making it inadequate clinical needs. This study developed rapid nucleic acid method based on scattering loop-mediated isothermal amplification (LAMP). Specific primers MTB-specific gene (Ag85B) were designed, LAMP reaction system was optimized using self-developed turbidimeter. Experimental results showed that optimal included 1.5 µL 100 mmol/L magnesium ions, 3.5 10 dNTPs, 6 1.6 mol/L betaine, temperature 65 °C. minimum limit 12.40 ng/L, with fastest time being approximately min. exhibited good specificity, no bands other pathogens. Twenty culture-positive samples twenty culture-negative tested parallel; positive group 100%, (24.9 ± 13 min), there negative detection. features high efficiency, low cost, accuracy, effectively reduces cross-contamination, providing new technology MTB.

Language: Английский

Citations

0
Enhancing bloodstream infection diagnostics: a novel filtration and targeted next-generation sequencing approach for precise pathogen identification DOI Creative Commons
Ting-Xuan Lin, Zihao Zhu,

Xiaohong Lin

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 20, 2025

Bloodstream infections (BSIs) pose a significant diagnostic challenge, largely due to the limitations of traditional methods such as blood cultures. These often yield low positive rates, have lengthy processing times that delay treatment, and are limited in detecting only narrow range pathogens. Such delays inaccuracies can critically impede timely clinical interventions, potentially compromising patient outcomes. Next-generation sequencing (NGS) is powerful tool for rapid, precise pathogen identification. While metagenomic NGS (mNGS) offers broad coverage, it costly complex. Targeted (tNGS), however, focuses on key regions clinically relevant pathogens, reducing costs simplifying workflows while maintaining high sensitivity, making more practical routine diagnostics. In this study, we introduce novel approach combining human cell-specific filtration membrane with multiplex tNGS panel overcome these challenges. The membrane, designed surface charge properties be electrostatically attractive leukocytes selective capture specific cells, demonstrated efficiency removing host cells nucleic acids, achieving over 98% reduction DNA thereby minimizing background interference detection. Additionally, developed an effective targeting 330 pathogens verified its consistency mNGS culture results, demonstrating improvement detection sensitivity. By integrating two methods, achieved synergistic enhancement capability, boosting reads by 6- 8-fold, which enabled reliable identification even cases low-abundance This provides faster, accurate, sensitive BSIs, enabling earlier infections. facilitates targeted ultimately improving outcomes critical care settings. Given unique strengths panel, shows promising applications prenatal genetic health support, well advancing early cancer screening strategies.

Language: Английский

Citations

0
Research on real-time nucleic acid detection device based on microfluidic technology DOI Open Access
Shuo Wu, Jianxin Cheng, Xiaohua Cao

et al.

International Journal on Smart Sensing and Intelligent Systems, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 1, 2025

Abstract In recent years, the point-of-care testing (POCT) device for nucleic acid detection has received extensive attention. POCT provides rapid, high sensitivity and accuracy of on-site diagnosis can quantify in clinical samples real time. The integration microfluidics ensures accurate fluid control, minimizes sample volume, improves efficiency. addition, devices conduct multichannel analysis, facilitate continuous monitoring, provide comprehensive quantitative data. Due to its portability, cost-effectiveness, ease use, represents a promising solution extensive, reliable, timely various medical environments. This review summarizes research development based on microfluidic technology trend prospected.

Language: Английский

Citations

0
Resonance-Induced Capacitively Coupled Contactless Conductivity Detection (ReC4D) Unit for Nucleic Acid Amplification Testing DOI Creative Commons
Roberto G. Ramírez-Chavarría, Jorge Alberto Uc-Martín, Bryan E. Alvarez-Serna

et al.

Technologies, Journal Year: 2025, Volume and Issue: 13(4), P. 138 - 138

Published: April 2, 2025

Nucleic acid amplification (NAA) is a technique that increases the number of copies gene, making it possible to detect microorganisms. This often used in clinical tests, biochemical analysis, and environmental assays, mention only few. However, developing portable, robust, low-cost measurement platforms evaluate NAA products remains technological challenge. Therefore, this work, we introduce an attractive unit for detecting quantifying nucleic acids based on capacitively coupled contactless conductivity detection (C4D) principle. The proposed unit, ReC4D, combines electrical resonance with C4D enhance sensitivity when evaluating reaction. ReC4D units advantages are twofold: (i) transducer electrically isolated allow its reuse, (ii) induced minimizes stray capacitances conventional which enhances sensitivity, linear operating range, improves limit (LoD). Furthermore, evaluated device different concentrations SARS-CoV-2 genetic material compared measurements from unit. Thus, demonstrate can measure LoD 0.24 copyμL 5.618 kHzlog(copyμL). These results position close state-of-the-art testing platforms, added value low cost, robustness, reusability, affordability.

Language: Английский

Citations

0
An open source, PCR based, point-of-care testing platform DOI Creative Commons

Yaping Xie,

Zhou Sha,

Shanshi Huang

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 8, 2025

Language: Английский

Citations

0
CRISPR/Cas and Argonaute-Based Biosensors for Nucleic acid Detection DOI
Qihua Xiong,

Cancan Zhu,

Xueer Yin

et al.

Talanta, Journal Year: 2025, Volume and Issue: 294, P. 128210 - 128210

Published: April 22, 2025

Language: Английский

Citations

0