Impact of acupuncture on ischemia/reperfusion injury: Unraveling the role of miR-34c-5p and autophagy activation DOI Creative Commons

Xiao-Ye Lu,

Q. Lv,

Qilong Li

et al.

Brain Research Bulletin, Journal Year: 2024, Volume and Issue: 215, P. 111031 - 111031

Published: July 11, 2024

We have previously reported that the expression of miR-34c-5p was up-regulated during acupuncture treatment in setting a cerebral ischemia/reperfusion injury (CIRI), indicating plays an important role healing from CIRI-induced brain injury. This study sought to evaluate effects on and autophagy forward reverse directions using rat focal model. After 120 minutes middle artery occlusion reperfusion, rats were treated with at "Dazhui" (DU20), "Baihui" (DU26) "Renzhong" (DU14) points. Neurologic function deficit score, infarct area ratio, neuronal apoptosis evaluated 72 hr after treatment. The agonist RAPA antagonist 3MA used neuro protective autophagy-mediated acupuncture. found improved tissue CIRI rats. Acupuncture reversed negative CIRI, combined further enhanced autophagy. also could increase hippocampal neurons ischemia/reperfusion. miR-34c agomir able enhance autophagy, improve neurologic deficits, reduce ratio rate by promoting miR-34c-5p. Silencing resulted significantly reduced activating effect increased apoptosis, symptoms, ratio. confirms can upregulate expression, which is beneficial CIRI.

Language: Английский

Acupuncture alleviates hemorrhagic transformation after delayed rt-PA treatment for acute ischemic stroke by regulating the mitophagy-NLRP3 inflammasome pathway DOI Creative Commons
Tao Jiang,

Qianqian Liu,

Huanhuan Liu

et al.

Frontiers in Neurology, Journal Year: 2025, Volume and Issue: 16

Published: April 7, 2025

The clinical application of recombinant tissue plasminogen activator (rt-PA) is significantly constrained by hemorrhagic transformation (HT), a common and severe complication following thrombolysis for ischemic stroke. Notably, the mitochondrial injury-mediated NLRP3 inflammasome plays crucial role in HT after delayed rt-PA acute Although acupuncture has demonstrated antioxidant anti-inflammatory effects cerebral infarction, its impact on thrombolysis, especially concerning mitophagy inflammasome, remains unclear. This study investigates how protects against resulting from damage activation We selected an embolic stroke model rats assessed brain injury using neurological deficit score, volume infarct, permeability assay blood-brain barrier (BBB), HT. Then, levels proteins mRNA involved pathway were measured western blot real-time PCR. interleukin-18 (IL-18) interleukin-1β (IL-1β) enzyme-linked immunosorbent (ELISA). Morphological changes BBB mitochondria neurons observed via transmission electron microscopy. Acupuncture improved scores, destruction, rat. Furthermore, induced substantially downregulated activity inflammasome. Additionally, use inhibitors reversed suppressive can promote suppress to decrease therapy

Language: Английский

Citations

0
Effect of DU meridian acupuncture and temporal three-needle on vascular protection mechanisms in patients with acute cerebral infarction: a randomized controlled evaluator-blinded clinical trial DOI Creative Commons
Ya-Wen Sun, Qin Zhong,

Yunfei Hou

et al.

Frontiers in Neurology, Journal Year: 2025, Volume and Issue: 16

Published: April 7, 2025

The incidence of acute ischemic stroke has been rising steadily in China and globally, with its high mortality disability rates significantly affecting quality life. As an important adjunct therapy, acupuncture widely implemented management. Emerging studies have investigated the effects DU meridian Chong Mai on hemiparesis; however, vascular protective mechanisms electroacupuncture therapy targeting these meridians require further elucidation rehabilitation research. This prospective cohort study aims to investigate clinical efficacy limitations combined Temporal Triple Needling (Sanjian) rehabilitation. intervention's therapeutic potential is evaluated through modulatory CD14+/CD14- monocyte subpopulations within peripheral blood mononuclear cells (PBMCs), particular focus angiogenesis-mediated protection mechanisms. Sixty-six patients will be randomly assigned 1:1 control (conventional basal n = 33) standard care + acupuncture, groups for 10 days intervention. primary outcome NIHSS score. secondary outcomes: BMI mRS scores, level TNF-A IL-1B serum, endothelial growth factor (VEGF), Endocan ES, CD14+ CD14- levels cells. aim this whether improves cerebrovascular function patients, thereby reducing score preventing disease progression. also contribute positively development relevant treatment protocols facilitate research into underlying effects. International Traditional Medicine Clinical Trial Registry ITMCTR2024000508.

Language: Английский

Citations

0
Acupuncture regulates astrocyte neurotoxic polarization to protect blood–brain barrier integrity in delayed thrombolysis through mediating ERK1/2/Cx43 axis DOI Creative Commons
Zhihui Zhang, Yu Gu, Zheng Huang

et al.

IBRO Neuroscience Reports, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Acupuncture in Conjunction with Modern Rehabilitation Promotes Neuroplasticity in Stroke DOI

玮豪 柯

Advances in Clinical Medicine, Journal Year: 2025, Volume and Issue: 15(04), P. 3322 - 3332

Published: Jan. 1, 2025

Language: Английский

Citations

0
Impact of acupuncture on ischemia/reperfusion injury: Unraveling the role of miR-34c-5p and autophagy activation DOI Creative Commons

Xiao-Ye Lu,

Q. Lv,

Qilong Li

et al.

Brain Research Bulletin, Journal Year: 2024, Volume and Issue: 215, P. 111031 - 111031

Published: July 11, 2024

We have previously reported that the expression of miR-34c-5p was up-regulated during acupuncture treatment in setting a cerebral ischemia/reperfusion injury (CIRI), indicating plays an important role healing from CIRI-induced brain injury. This study sought to evaluate effects on and autophagy forward reverse directions using rat focal model. After 120 minutes middle artery occlusion reperfusion, rats were treated with at "Dazhui" (DU20), "Baihui" (DU26) "Renzhong" (DU14) points. Neurologic function deficit score, infarct area ratio, neuronal apoptosis evaluated 72 hr after treatment. The agonist RAPA antagonist 3MA used neuro protective autophagy-mediated acupuncture. found improved tissue CIRI rats. Acupuncture reversed negative CIRI, combined further enhanced autophagy. also could increase hippocampal neurons ischemia/reperfusion. miR-34c agomir able enhance autophagy, improve neurologic deficits, reduce ratio rate by promoting miR-34c-5p. Silencing resulted significantly reduced activating effect increased apoptosis, symptoms, ratio. confirms can upregulate expression, which is beneficial CIRI.

Language: Английский

Citations

1