pir-hsa-216911 inhibit pyroptosis in hepatocellular carcinoma by suppressing TLR4 initiated GSDMD activation

Cell Death Discovery, Journal Year: 2025, Volume and Issue: 11(1)

Published: Jan. 17, 2025

Hepatocellular carcinoma (HCC) is a global health concern, ranking as the fourth leading cause of cancer-related deaths worldwide. However, role piwi-interacting RNAs (piRNAs) in HCC processes has not been extensively explored. Through small RNA sequencing, our study identified specific piRNA, pir-hsa-216911, which highly expressed cells. This overexpression pir-hsa-216911 promotes cell invasion and inhibits death, particularly pyroptosis. Knocking out led to increased pyroptosis activity, resulting activation caspase-1 GSDMD. Further analysis revealed that targets suppresses TLR4, key gene associated with HCC. In Huh7 line, knockout confirmed its suppressing TLR4/NFκB/NLRP3 pathway by silencing TLR4. significantly inhibited formation xenograft tumor. patients, was tumor samples steatosis, TLR4 expression inhibiting GSDMD activation. introduces new high-expressing piRNA HCC, suppress These findings have significant implications for molecular subtyping potential target cancer therapy.

Language: Английский

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Human liver cancer organoids: Biological applications, current challenges, and prospects in hepatoma therapy

Cancer Letters, Journal Year: 2023, Volume and Issue: 555, P. 216048 - 216048

Published: Jan. 2, 2023

Language: Английский

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Organoids and spheroids: advanced in vitro models for liver cancer research

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 9, 2025

Liver cancer is a leading cause of cancer-related deaths worldwide, highlighting the need for innovative approaches to understand its complex biology and develop effective treatments. While traditional in vivo animal models have played vital role liver research, ethical concerns demand more human-relevant systems driven development advanced vitro models. Spheroids organoids emerged as powerful tools due their ability replicate tumor microenvironment facilitate preclinical drug development. are simpler 3D culture that partially recreate structure cell interactions. They can be used penetration studies high-throughput screening. Organoids derived from stem cells or patient tissues accurately emulate complexity functionality tissue. generated pluripotent adult cells, well specimens, providing personalized studying behavior responses. retain genetic variability original offer robust platform screening treatment strategies. However, both spheroids limitations, such absence functional vasculature immune components, which essential growth therapeutic The field modeling evolving, with ongoing efforts predictive reflect complexities human cancer. By integrating these tools, researchers gain deeper insights into accelerate novel

Language: Английский

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NANOTECHNOLOGY-DRIVEN THERAPEUTICS FOR LIVER CANCER: CLINICAL APPLICATIONS AND PHARMACEUTICAL INSIGHTS

Asian Journal of Pharmaceutical and Clinical Research, Journal Year: 2025, Volume and Issue: unknown, P. 8 - 26

Published: Feb. 7, 2025

Hepatocellular carcinoma (HCC) represents a significant threat to global health and is responsible for mortality rates worldwide. Conventional treatment options such as surgery chemotherapy have inherent limitations. In order remedy these deficits, the development of novel therapeutic strategies essential. Nanomedicines shown promise in HCC they offer improved stability, controlled release, increased drug loading capacity. This review explores application nanoconstructs treatment, including active passive targeting strategies. addition, liver cell approaches, moieties, conjugation chemistry surface functionalization are investigated. A compact overview various approaches also given.

Language: Английский

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Nanoparticle drug delivery systems in hepatocellular carcinoma: A focus on targeting strategies and therapeutic applications

OpenNano, Journal Year: 2023, Volume and Issue: 12, P. 100159 - 100159

Published: May 6, 2023

Hepatocellular carcinoma (HCC) is recognized as a global health issue accounting for millions of deaths every year. Surgery, liver ablation, and embolization therapy are amongst the conventional methods treatment HCC. Chemotherapy plays major role in HCC therapy, however, owing to its pharmacotherapy limitations, it necessitates development novel therapeutic strategies. In contrast, nanomedicines have shown remarkable prospects solving these complications their high stability, controlled release, drug loading capacity. This review gives an insight into nano-constructs used active passive targeting also inculcates various approaches cells, moieties conjugation chemistries involved surface functionalization. A brief description has been discussed.

Language: Английский

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Three Dimensional Bioprinting for Hepatic Tissue Engineering: From In Vitro Models to Clinical Applications

Tissue Engineering and Regenerative Medicine, Journal Year: 2023, Volume and Issue: 21(1), P. 21 - 52

Published: Oct. 26, 2023

Abstract Fabrication of functional organs is the holy grail tissue engineering and possibilities repairing a partial or complete liver to treat chronic disorders are discussed in this review. Liver largest gland human body plays responsible role majority metabolic function processes. Chronic disease one leading causes death globally current treatment strategy organ transplantation holds its own demerits. Hence there need develop an vitro model that mimics native microenvironment. The developed should be reliable understand pathogenesis, screen drugs assist repair replace damaged liver. three-dimensional bioprinting promising technology recreates vivo alike for transplantation, which goal engineers. has great potential due precise control ability homogeneously distribute cells on all layers complex structure. This review gives overview with special focus 3D bioinks modelling drug screening.

Language: Английский

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Bioprinting of hepatic tissue using 3D technology: Transitioning beyond laboratory models to real-world applications in medical treatments

Applied Materials Today, Journal Year: 2024, Volume and Issue: 39, P. 102307 - 102307

Published: July 8, 2024

Language: Английский

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Liver Organoids as an In Vitro Model to Study Primary Liver Cancer

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(5), P. 4529 - 4529

Published: Feb. 25, 2023

Primary liver cancers (PLC), including hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA), are among the leading causes of cancer-related mortality worldwide. Bi-dimensional in vitro models unable to recapitulate key features PLC; consequently, recent advancements three-dimensional systems, such as organoids, opened up new avenues for development innovative studying tumour’s pathological mechanisms. Liver organoids show self-assembly self-renewal capabilities, retaining essential aspects their respective vivo tissue allowing modelling diseases personalized treatment development. In this review, we will discuss current advances field focusing on existing protocols possible applications regenerative medicine drug discovery.

Language: Английский

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From Non-Alcoholic Fatty Liver Disease to Liver Cancer: Microbiota and Inflammation as Key Players

Pathogens, Journal Year: 2023, Volume and Issue: 12(7), P. 940 - 940

Published: July 15, 2023

It is estimated that 25% of the world's population has non-alcoholic fatty liver disease. This disease can advance to a more severe form, steatohepatitis (NASH), with greater probability progression cirrhosis and hepatocellular carcinoma (HCC). NASH could be characterized as necro-inflammatory complication chronic hepatic steatosis. The combination factors lead its HCC in setting inflammation not clearly understood. portal vein main route communication between intestine liver. allows transfer products derived from response pathway bile antibody secretion intestine. intestinal microbiota performs fundamental role regulation immune function, but it undergo changes alter functionality. These also contribute cancer by disrupting system causing dysfunction, both which are implicated development. In this article, we address link inflammation, HCC. We review different vitro models, well recent clinical trials addressing microbiota.

Language: Английский

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Glutathione and Selenium Supplementation Attenuates Liver Injury in Diethylnitrosamine-Induced Hepatocarcinogenic Mice by Enhancing Glutathione-Related Antioxidant Capacities

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11339 - 11339

Published: Oct. 22, 2024

Excess oxidative stress and inadequate antioxidant capacities are critical features in the development of hepatocellular carcinoma. This study aimed to determine whether supplementation with glutathione (GSH) and/or selenium (Se), as antioxidants, attenuates diethylnitrosamine (DEN)-induced hepatocarcinogenesis mice. C57BL/6J male mice were randomly assigned control, DEN, DEN + GSH, Se, GSH Se groups for 20 weeks. Daily commenced first experimental week continued throughout study. was administered weeks 2-9 16-19 period. administration induced significant pathological alterations hepatic foci, evidenced by elevated levels liver function, accompanied high malondialdehyde (MDA) levels; low peroxidase (GPx), reductase (GR),

Language: Английский

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