Analytical Biochemistry, Journal Year: 2024, Volume and Issue: 696, P. 115686 - 115686
Published: Oct. 10, 2024
Language: Английский
Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14
Published: Jan. 25, 2024
Circulating tumor cells (CTCs), circulating DNA (ctDNA), and extracellular vehicles (EVs) have received significant attention in recent times as emerging biomarkers subjects of transformational studies. The three main branches liquid biopsy evolved from the primary detection targets—CTC, ctDNA, EVs—each with distinct benefits. CTCs are derived cancer original or metastases may display global features tumor. ctDNA has been extensively analyzed used to aid diagnosis, treatment, prognosis neoplastic diseases. EVs contain tumor-derived material such DNA, RNA, proteins, lipids, sugar structures, metabolites. provide different contents but strong complementarity a certain extent. Even though they already employed several clinical trials, utility is still being studied, promising initial findings. This review thoroughly overviews established technologies for isolation, characterization, content CTC, EVs. Also discussed were most developments study potential therapeutic monitoring, prediction. These included Finally, challenges employing based on precision medicine evaluated.
Language: Английский
Citations
30
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 802 - 802
Published: Jan. 18, 2025
Prostate cancer, a leading cause of cancer-related mortality among men, often presents challenges in accurate diagnosis and effective monitoring. This systematic review explores the potential exosomal biomolecules as noninvasive biomarkers for diagnosis, prognosis, treatment response prostate cancer. A thorough literature search through online public databases (Medline via PubMed, Scopus, Web science) using structured terms screening predefined eligibility criteria resulted 137 studies that we analyzed this review. We evaluated findings from these clinical studies, revealing load exosomes blood urine cancer patients, which includes microRNAs (miRNAs), proteins, lipids, demonstrates disease-specific changes. It also shows some markers can differentiate between malignant benign hyperplasia prostate, predict disease aggressiveness, monitor efficacy. Notably, miRNA emerged most frequently studied biomolecule, demonstrating superior diagnostic compared to traditional methods like prostate-specific antigen (PSA) testing. The analysis highlights pressing need standardised analytic approach multi-centre validate full monitoring
Language: Английский
Citations
3
Medicina, Journal Year: 2023, Volume and Issue: 59(4), P. 724 - 724
Published: April 7, 2023
The incidence of testicular cancer is steadily increasing over the past several decades in different developed countries. If on one side better diagnosis and treatment have shone a light this disease, other side, differently from malignant diseases, few risk factors been identified. reasons for increase are however unknown while still poorly understood. Several studies suggested that exposure to various adolescence as well adulthood could be linked development cancer. Nevertheless, role environment, infections, occupational undoubtedly associated with an or decrease risk. aim narrative review summarize most recent evidence regarding cancer, starting commonly evaluated (cryptorchidism, family history, infections) newer identified hypothesized factors.
Language: Английский
Citations
38
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 3867 - 3867
Published: March 30, 2024
Renal cell carcinoma (RCC) remains a formidable diagnostic challenge, especially in the context of small renal masses. The quest for non-invasive screening tools and biomarkers has steered research towards liquid biopsy, focusing on microRNAs (miRNAs), exosomes, circulating tumor cells (CTCs). MiRNAs, non-coding RNAs, exhibit notable dysregulation RCC, offering promising avenues diagnosis prognosis. Studies underscore their potential across various biofluids, including plasma, serum, urine, RCC detection subtype characterization. Encouraging miRNA signatures show correlations with overall survival, indicative future relevance management. Exosomes, diverse molecular cargo, miRNAs, emerge as enticing biomarkers, while CTCs, emanating from primary tumors into bloodstream, provide valuable insights cancer progression. Despite these advancements, clinical translation necessitates further validation standardization, encompassing larger-scale studies robust evidence generation. Currently lacking approved assays cancer, applications biopsy follow-up care, treatment selection, outcome prediction patients are profound. This review aims to discuss highlight recent advancements exploring strengths weaknesses comprehensive management this disease.
Language: Английский
Citations
9
Cancers, Journal Year: 2023, Volume and Issue: 15(12), P. 3140 - 3140
Published: June 10, 2023
Prostate cancer (PCa) is the most frequently diagnosed and second leading cause of deaths among American men. Complex genetic epigenetic mechanisms are involved in development progression PCa. MicroRNAs (miRNAs) short noncoding RNAs that regulate protein expression at post-transcriptional level by targeting mRNAs for degradation or inhibiting translation. In past two decades, field miRNA research has rapidly expanded, emerging evidence revealed dysfunction to be an important mechanism underlying a wide range diseases, including cancers. This review article focuses on understanding functional roles molecular deregulated miRNAs PCa aggressiveness drug resistance based existing literature. Specifically, differentially expressed (upregulated downregulated) vs. normal tissues, advanced low-grade PCa, treatment-responsive non-responsive discussed. particular, oncogenic tumor-suppressive regulation (1) synthesis androgen receptor (AR) its AR-V7 splice variant, (2) PTEN PTEN-mediated signaling, (3) RNA splicing mechanisms, (4) chemo- hormone-therapy resistance, (5) racial disparities discussed summarized. We further provide overview current advances challenges miRNA-based biomarkers therapeutics clinical practice diagnosis/prognosis treatment.
Language: Английский
Citations
20
Molecular Therapy — Oncolytics, Journal Year: 2023, Volume and Issue: 30, P. 27 - 38
Published: July 19, 2023
Prostate cancer (PCa) incidence is increasing and endangers men's lives. Early detection of PCa could improve overall survival (OS) by preventing metastasis. The prostate-specific antigen (PSA) test a popular screening method. Several advisory groups, however, warn against using the PSA due to its high false positive rate, unsupported outcome, limited benefit. number disease-related biopsies performed annually far outweighs diagnoses. Thus, there an urgent need develop accurate diagnostic biomarkers detect distinguish between aggressive indolent cancers. Recently, non-coding RNA (ncRNA), circulating tumor DNA (ctDNA)/ctRNA, exosomes, metabolomic in liquid (LBs) patients with showed significant differences clinical benefits diagnosis, prognosis, monitoring response therapy. analysis urinary exosomal ncRNA presented substantial correlation among Exos-miR-375 downregulation, T stage, bone metastases PCa. Furthermore, expression miR-532-5p urine samples was vital predictive biomarker progression. this review focuses on promising molecular LBs from We thoroughly addressed most recent findings LB use diagnosing early advanced stages.
Language: Английский
Citations
18
Diagnostics, Journal Year: 2023, Volume and Issue: 13(4), P. 806 - 806
Published: Feb. 20, 2023
Prostate cancer is the second leading cause of cancer-related death in men. Its early and correct diagnosis particular importance to controlling preventing disease from spreading other tissues. Artificial intelligence machine learning have effectively detected graded several cancers, prostate cancer. The purpose this review show diagnostic performance (accuracy area under curve) supervised algorithms detecting using multiparametric MRI. A comparison was made between performances different machine-learning methods. This study performed on recent literature sourced scientific citation websites such as Google Scholar, PubMed, Scopus, Web Science up end January 2023. findings reveal that techniques good with high accuracy curve for prediction MR imaging. Among methods, deep learning, random forest, logistic regression appear best performance.
Language: Английский
Citations
17
Diagnostics, Journal Year: 2023, Volume and Issue: 13(11), P. 1939 - 1939
Published: June 1, 2023
: Multiparametric magnetic resonance is an established imaging utilized in the diagnostic pathway of prostate cancer. The aim this study to evaluate accuracy and reliability multiparametric (mpMRI) detection clinically significant cancer, defined as Gleason Score ≥ 4 + 3 or a maximum cancer core length 6 mm longer, patients with previous negative biopsy.
Language: Английский
Citations
17
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5955 - 5955
Published: May 29, 2024
Bladder cancer (BC) is a common genitourinary malignancy that exhibits silent morbidity and high mortality rates because of lack diagnostic markers limited effective treatments. Here, we evaluated the role lncRNA brain cytoplasmic RNA 1 (BCYRN1) in BC. We performed loss-of-function assays to examine effects BCYRN1 downregulation T24 BOY BC cells. found significantly inhibited proliferation, migration, invasion, three-dimensional spheroid formation ability induced apoptosis Additionally, gene set enrichment analysis (GSEA) using sequences from tumor fractions showed decreased expression mRNAs associated with cell cycle. These findings were supported by observations G2/M arrest flow cytometry assays. Finally, examined serum exosomal as biomarker. Clinically, exosomes patients (n = 31) was higher than healthy donors 19; mean difference: 4.1-fold higher, p < 0.01). Moreover, who had undergone complete resection BC, levels 8). Thus, may be promising marker therapeutic target
Language: Английский
Citations
7
Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14
Published: March 17, 2023
Introduction Bladder cancer (BLCA) is one of the most lethal diseases. COL10A1 secreted small-chain collagen in extracellular matrix associated with various tumors, including gastric, colon, breast, and lung cancer. However, role BLCA remains unclear. This first research focusing on prognostic value BLCA. In this research, we aimed to uncover association between prognosis, as well other clinicopathological parameters Methods We obtained gene expression profiles normal tissues from TCGA, GEO, ArrayExpress databases. Immunohistochemistry staining was performed investigate protein patients. GO KEGG enrichment along GSEA analyses were reveal biological functions potential regulatory mechanisms based co-expression network. used “maftools” R package display mutation high low groups. GIPIA2, TIMER, CIBERSORT algorithms utilized explore effect tumor immune microenvironment. Results found that upregulated samples, increased related poor overall survival. Functional annotation 200 co-expressed genes positively correlated expression, GO, KEGG, analyses, indicated basically involved matrix, modification, molecular binding, ECM-receptor interaction, digestion absorption, focal adhesion, PI3K-Akt signaling pathway. The commonly mutated different Tumor infiltrating showed might have an essential recruiting cells regulating immunity BLCA, thus affecting prognosis. Finally, external datasets biospecimens used, results further validated aberrant samples. Conclusions conclusion, our study demonstrates underlying predictive biomarker
Language: Английский
Citations
16