Concurrent Oncolysis and Neurolesion Repair by Dual Gene-Engineered hNSCs in an Experimental Model of Intraspinal Cord Glioblastoma DOI Creative Commons
Xiang Zeng,

Alexander E. Ropper,

Zaid Aljuboori

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(18), P. 1522 - 1522

Published: Sept. 11, 2024

Intramedullary spinal cord glioblastoma (ISCG) is lethal due to lack of effective treatment. We previously established a rat C6-ISCG model and the antitumor effect F3.CD-TK, an hNSC line expressing CD TK, via producing cytocidal 5FU GCV-TP. However, neurotherapeutic potential this approach has remained uninvestigated. Here for first time, cultured F3.CD-TK cells were found have markedly higher oncolytic effect, which was GJIC-dependent, BDNF expression but less VEGF secretion than F3.CD. In Rowett athymic rats, (1.5 × 10

Language: Английский

Glioma-associated macrophages: unraveling their dual role in the microenvironment and therapeutic implications DOI Creative Commons
Jiachen Wang, Shenglan Li,

Yanjie Lan

et al.

Current Medicine, Journal Year: 2024, Volume and Issue: 3(1)

Published: April 7, 2024

Abstract Glioblastoma (GBM) is a malignant brain glioma characterized by high number of tumor-associated macrophages (TAMs) within its tissues. These TAMs have close relationship with tumor grade and prognosis. Targeting has been identified as promising therapeutic strategy. However, TAM cells play both tumor-killing tumor-promoting roles, making them double-edged sword in the immune environment. The different subtypes their effects on microenvironment remain poorly understood. This study comprehensively elucidates immunobiology glioma-associated (GAMs), including origin, classification, molecular mechanisms underlying promotion inhibition, polarization strategies, targeted therapy for GAMs current challenges perspectives modulation. Further research macrophage function mechanism may provide new immunological basis treating GBM patients enhancing efficacy immunotherapy.

Language: Английский

Citations

10
Tumor-associated microenvironment, PD-L1 expression and their relationship with immunotherapy in glioblastoma, IDH-wild type: A comprehensive review with emphasis on the implications for neuropathologists DOI Creative Commons
Giuseppe Broggi, Giuseppe Angelico, Jessica Farina

et al.

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 254, P. 155144 - 155144

Published: Jan. 21, 2024

Although novel knowledge has been acquired on the molecular landscape of glioblastoma (GBM), a relatively few steps forward have made regarding its therapy. With increasing use immunotherapeutic drugs capable stimulating antitumor inflammatory response, in last decades numerous studies aimed to characterize tumor-associated microenvironment (TME) and relationship with immunogenicity GBM. In this regard, although microglia macrophages (TAMs) PD-L1/PD-1 axis emerged as one most relevant components GBM TME potential pathways targetable immunotherapy, respectively. It supposed that TAMs may acquire different phenotypes, switching from M1 M2 tumor-suppressive tumor-stimulating role depending surrounding conditions. PD-L1 is type 1 transmembrane protein ligand expressed by T-cells, B-cells antigen-presenting cells, main inhibitory checkpoint tumor immune regulation. While immunohistochemical expression extensively investigated many cancers, usefulness evaluation response rates immunotherapy standardized immunohistochemistry are still debated. The present review paper focuses current "state art" about between TME, pathway GBM, also providing neuropathologists an updated guide clinical trials conducted PD-1 inhibitors.

Language: Английский

Citations

6
Treatment-aware Diffusion Probabilistic Model for Longitudinal MRI Generation and Diffuse Glioma Growth Prediction DOI Creative Commons
Qinghui Liu, Elies Fuster‐García, Ivar Thokle Hovden

et al.

IEEE Transactions on Medical Imaging, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 1

Published: Jan. 1, 2025

Diffuse gliomas are malignant brain tumors that grow widespread through the brain.The complex interactions between neoplastic cells and normal tissue, as well treatment-induced changes often encountered, make glioma tumor growth modeling challenging.In this paper, we present a novel end-to-end network capable of future predictions masks multi-parametric magnetic resonance images (MRI) how will look at any time points for different treatment plans.Our approach is based on cutting-edge diffusion probabilistic models deep-segmentation neural networks.We included sequential MRI information conditioning inputs to guide generative process joint segmentation process.This allows estimates realistic generation given point.We trained model using real-world postoperative longitudinal data with trajectories represented maps over time.The demonstrates promising performance across various tasks, including generating high-quality masks, performing time-series segmentations, providing uncertainty estimates.Combined treatment-aware generated MRI, can provide useful clinical decision-making.

Language: Английский

Citations

0
An Early Progression Biomarker in Glioblastoma: Microcirculatory Heterogeneity on Ultrasound Localization Microscopy DOI
Xing Hu, Gaobo Zhang, Xiandi Zhang

et al.

Ultrasound in Medicine & Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Translocator protein (TSPO) in glioma: implications for diagnosis, disease progression monitoring, and targeted therapies DOI Creative Commons

Julius Mulumba,

Bo Luo, Jiang Wu

et al.

Exploration of neuroscience, Journal Year: 2025, Volume and Issue: 4

Published: April 1, 2025

Glioma is a highly aggressive brain cancer associated with significant mortality. Despite advances in diagnostic and therapeutic strategies, the prognosis for glioma patients remains poor due to limited accuracy monitoring capabilities. Translocator protein (TSPO) mitochondrial implicated various cancers, including glioma, where it plays role cell survival, proliferation, chemo-resistance. This review article aimed comprehensively analyze of TSPO particularly its potential applications enhancing methods strategies. Molecular imaging techniques have emerged as promising tools non-invasive diagnosis, disease progression monitoring, treatment selection gliomas. A comprehensive literature was conducted explore TSPO’s expression patterns, biological functions, molecular imaging. Studies utilizing positron emission tomography (PET), single photon computed (SPECT), magnetic resonance (MRI), other modalities were included. overexpressed cells, high-grade tumors, correlating tumor aggressiveness patient prognosis. TSPO-targeted agents demonstrate high specificity sensitivity detection, positioning marker accurate diagnosis monitoring. Future studies should focus on optimizing protocols, validating their clinical utility, exploring combined improve precision.

Language: Английский

Citations

0
Phagocytosis Checkpoints in Glioblastoma: CD47 and Beyond DOI Creative Commons
Amber Afzal, Zobia Afzal,

Sophia Bizink

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(8), P. 7795 - 7811

Published: July 23, 2024

Glioblastoma multiforme (GBM) is one of the deadliest human cancers with very limited treatment options available. The malignant behavior GBM manifested in a tumor which highly invasive, resistant to standard cytotoxic chemotherapy, and strongly immunosuppressive. Immune checkpoint inhibitors have recently been introduced clinic yielded promising results certain cancers. GBM, however, largely refractory these treatments. immune CD47 has gained attention as potential target for intervention it conveys “don’t eat me” signal tumor-associated macrophages (TAMs) via inhibitory SIRP alpha protein. In preclinical models, administration anti-CD47 monoclonal antibodies shown impressive other models. Several well-characterized oncogenic pathways regulate expression cells glioma stem (GSCs) including Epidermal Growth Factor Receptor (EGFR) beta catenin. Other macrophage involved regulating phagocytosis TREM2 glycan binding proteins are discussed well. Finally, chimeric antigen receptor (CAR-Ms) could be leveraged greatly enhancing repolarization microenvironment general. Here, we comprehensively review mechanisms that cells.

Language: Английский

Citations

2
Comprehensive Transcriptomic Profiling of Diverse Brain Tumor Types Uncovers Complex Structures of the Brain Tumor Microenvironment DOI Creative Commons
Jiin Choi, Hee Jin Cho

Biomedicines, Journal Year: 2024, Volume and Issue: 12(3), P. 506 - 506

Published: Feb. 23, 2024

Various types of brain tumors occur in both children and adults. These manifest with different characteristics such as malignancy, cellular lineage, location origin, genomic profile. Recently, immunotherapy, which manipulates immune cells the tumor microenvironment (TME) to kill cells, has attracted attention a treatment strategy for tumors. Here, we analyzed transcriptomic architecture provide potential guidelines overcome therapeutic vulnerabilities We decomposed populations six (meningioma, pilocytic astrocytoma, ependymoma, medulloblastoma, glioblastoma, lower-grade glioma) using publicly available microarray data single-cell RNA sequencing (scRNA-seq) data. Interestingly, transcriptome-based cell profiling revealed that infiltrating TME, particularly M2 macrophages, CD8+ T CD4+ could be distinguished by type, location. scRNA-seq differences proportions dendritic mural cells. Unsupervised clustering immune-related genes divided all samples into two distinct clusters characteristics. In addition, subpopulations showed disparate reactions after anti-PD-1 therapy glioblastoma. Our results unveiled TME across provided landscape. findings may contribute realizing future precision medicine, providing basic rationale therapeutics

Language: Английский

Citations

1
GNB4 Silencing Promotes Pyroptosis to Inhibit the Development of Glioma by Activating cGAS–STING Pathway DOI

Zhiqiang Gao,

Jing Yang

Molecular Biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: May 30, 2024

Language: Английский

Citations

1
The Biological and Clinical Role of the Telomerase Reverse Transcriptase Gene in Glioblastoma: A Potential Therapeutic Target? DOI Creative Commons
Vincenzo Di Nunno,

Marta Aprile,

Stefania Bartolini

et al.

Cells, Journal Year: 2023, Volume and Issue: 13(1), P. 44 - 44

Published: Dec. 25, 2023

Glioblastoma IDH-wildtype represents the most lethal and frequent primary tumor of central nervous system. Thanks to important scientific efforts, we can now investigate its deep genomic assessment, elucidating mutated genes altered biological mechanisms in addition clinical aggressiveness. The telomerase reverse transcriptase gene (TERT) is frequently solid tumors, including brain tumors GBM IDH-wildtype. In particular, it be observed approximately 80–90% cases. Its clonal distribution on almost all cancer cells makes this an optimal target. However, research effective TERT inhibitors complicated by several obstacles which only partially surmounted. Very recently, novel immunological approaches leading inhibition have been investigated, offering potential develop target for protein. Here, perform a narrative review investigating role alterations glioblastoma principal associated with inhibitions population. Moreover, discuss possible combination treatment strategies overcome these limitations.

Language: Английский

Citations

3
Concurrent Oncolysis and Neurolesion Repair by Dual Gene-Engineered hNSCs in an Experimental Model of Intraspinal Cord Glioblastoma DOI Creative Commons
Xiang Zeng,

Alexander E. Ropper,

Zaid Aljuboori

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(18), P. 1522 - 1522

Published: Sept. 11, 2024

Intramedullary spinal cord glioblastoma (ISCG) is lethal due to lack of effective treatment. We previously established a rat C6-ISCG model and the antitumor effect F3.CD-TK, an hNSC line expressing CD TK, via producing cytocidal 5FU GCV-TP. However, neurotherapeutic potential this approach has remained uninvestigated. Here for first time, cultured F3.CD-TK cells were found have markedly higher oncolytic effect, which was GJIC-dependent, BDNF expression but less VEGF secretion than F3.CD. In Rowett athymic rats, (1.5 × 10

Language: Английский

Citations

0