Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 18, 2024
Almost
all
proteins
on
the
cell
surface
are
modified
by
glycosylation.
Cell
glycoproteins
participate
in
various
cellular
pathways,
such
as
adhesion,
cell–cell
communication,
and
immune
response.
Due
to
their
functional
importance,
often
serve
potential
therapeutic
targets.
Recent
advancements
mass
spectrometry
(MS)
have
facilitated
characterization
of
that
generally
localized
surface,
secreted
extracellular
environment,
or
found
intracellular
organelles
endoplasmic
reticulum,
Golgi
apparatus,
peroxisome.
However,
selective
remains
challenging.
In
this
study,
we
applied
enzymatic
treatment
live
cells,
followed
MS-based
glycoproteomics
analysis,
assess
changes
protein
glycosylation
at
different
time
points
a
method
identify
glycoproteins.
To
demonstrate
approach,
renal
carcinoma
line,
A498,
was
treated
with
glycosidases,
sialidase
PNGase
F,
over
two
intervals,
2
24
h.
Glycoproteins
were
identified
from
A498
cells
when
enzyme
altered
The
results
revealed
effectiveness
integrating
for
analyzing
Our
established
has
demonstrated
applications
assessing
accessibility
targets
supporting
development
new
targeted
therapies.
Viruses,
Journal Year:
2025,
Volume and Issue:
17(2), P. 218 - 218
Published: Feb. 1, 2025
Cancer
is
a
major
global
health
problem
that
poses
significant
challenges.
Conventional
cancer
therapies
often
have
severe
side
effects,
necessitating
the
development
of
novel
therapeutic
approaches
are
more
effective
and
less
toxic.
The
utilization
plant
viral
nanoparticles
one
promising
strategies
for
biotherapy.
Plant
exhibit
advantageous
properties,
including
safety,
high
stability,
rapid
production
scalability,
biocompatibility
biodegradability,
structural
uniformity,
inherent
immunogenicity,
ease
modification
update
efficacy
as
well
lower
cost
implications,
making
them
attractive
vehicles
applications.
Various
studies
demonstrated
in
targeted
drug/molecule
delivery,
tumor
imaging
immunotherapy,
highlighting
their
potential
versatile
platform
drawbacks
include
perceived
ability
to
induce
hypersensitive/allergic
immune
response,
non-well-defined
regulatory
approval
processes
reluctance
pharmaceutical
companies
adapt
manufacturing
facilitate
plant-based
expression.
This
review
discusses
applications
virus-derived
therapeutics
prospects
translating
these
findings
into
clinical
practice.
Cells,
Journal Year:
2024,
Volume and Issue:
13(5), P. 395 - 395
Published: Feb. 25, 2024
The
aberrant
glycosylation
is
a
hallmark
of
cancer
progression
and
chemoresistance.
It
also
an
immune
therapeutic
target
for
various
cancers.
Tunicamycin
(TM)
one
the
potent
nucleoside
antibiotics
inhibitor
in
cells,
including
breast
cancer,
gastric
pancreatic
parallel
with
inhibition
cell
growth
tumors.
Like
chemotherapies
such
as
doxorubicin
(DOX),
5′fluorouracil,
etoposide,
cisplatin,
TM
induces
unfolded
protein
response
(UPR)
by
blocking
glycosylation.
Consequently,
stress
induced
endoplasmic
reticulum
(ER)
that
promotes
apoptosis.
can
thus
be
considered
antitumor
drug
cancers
may
promote
chemosensitivity.
However,
its
lack
cell-type-specific
cytotoxicity
impedes
anticancer
efficacy.
In
this
review,
we
focus
on
recent
advances
our
understanding
benefits
pitfalls
therapies
cancers,
breast,
colon,
discuss
mechanisms
identified
which
functions.
Finally,
potential
use
nano-based
delivery
systems
to
overcome
non-specific
toxicity
enhance
efficacy
targeted
therapy.
International Journal of Biological Sciences,
Journal Year:
2024,
Volume and Issue:
20(7), P. 2607 - 2621
Published: Jan. 1, 2024
Immunotherapy
has
shown
great
potential
in
cancer
treatment.However,
even
with
the
intervention
of
techniques
such
as
immune
checkpoint
inhibitor
therapy,
tumors
can
still
achieve
escape,
leading
to
a
low
response
rate.Abnormal
glycosylation
is
widely
recognized
hallmark
cancer.The
development
complex
"glyco-code"
on
surface
tumor
cells
potentially
influence
system's
ability
monitor
and
impact
anti-tumor
response.Therefore,
abnormal
emerged
promising
target
for
immunotherapy.Many
recent
studies
have
that
targeted
reshape
microenvironment
(TME)
promote
response,
thereby
improving
immunotherapy.This
review
summarizes
how
affects
function
TME
synthesizes
latest
research
progress
immunotherapy.It
hoped
by
elucidating
basic
laws
biological
connotations
glycosylation,
this
will
enable
researcher
thoroughly
analyze
mechanism
its
metabolic
regulation
network,
which
provide
theoretical
tool
promoting
clinical
application
codes.
The
aberrant
glycosylation
is
a
hallmark
of
cancer
progression
and
chemoresistance.
It
also
an
immune
therapeutic
target
for
various
cancers.
Tunicamycin
(TM)
one
the
potent
nucleo-side
antibiotics
inhibitor
in
cells,
including
breast
cancer,
gastric
pancreatic
parallel
with
inhibition
cell
growth
tumors.
Thus,
TM
can
be
considered
antitumor
drug
cancers
may
promote
chemosensitivity.
Mechanistically,
impedes
role
UDP-HexNAc
enzyme
biosynthesis
oligosaccharides,
specialized
macromolecules
instrumental
N-linked
glycosylation.
Further,
like
chemotherapies
such
as
doxorubicin
(DOX),
5'fluorouracil,
etoposide,
Cisplatin,
induces
unfolded
protein
response
(UPR)
by
blocking
Despite
TM's
effectiveness,
its
lack
cell-type
specific
cytotoxicity
anticancer
efficacy.
nanoencapsulation
techniques
materials
have
been
use
experiments
to
reduce
improve
efficacy
targeted
therapy.
current
review
profound
audit
benefits
pitfalls
cancers,
focusing
on
breast,
colon,
Additional
progressive
studies
discussed
checkpoints
other
unique
pathways.
Cytotoxicity
possibly
adverse
effects
are
highlighted
based
data
from
vitro
vivo
assays.
In
addition,
recent
advances
nano-based
delivery
systems
regarding
emphasized.
However,
potential
this
nucleoside
re-quires
thorough
investigation
research
determine
likeliness
viable
chemotherapeutic.
Cancers,
Journal Year:
2024,
Volume and Issue:
16(7), P. 1334 - 1334
Published: March 29, 2024
Siglecs
play
a
key
role
in
mediating
cell–cell
interactions
via
the
recognition
of
different
sialylated
glycoconjugates,
including
tumor-associated
MUC1,
which
can
lead
to
activation
or
inhibition
immune
response.
The
occurs
through
signaling
with
cytoplasmic
immunoreceptor
tyrosine-based
motif
(ITAM)-containing
proteins,
while
signal
is
result
interaction
intracellular
(ITIM)-bearing
receptors.
MUC1
glycans
ITIM
motifs
decreases
antitumor
immunity.
Consequently,
these
are
expected
tumor
evasion.
Efforts
modulate
response
cells
by
blocking
immune-suppressive
effects
inhibitory
Siglecs,
driving
immune-activating
and/or
altering
synthesis
and
expression
sialic
acid
glycocalyx
new
therapeutic
strategies
deserving
further
investigation.
We
will
highlight
Siglec’s
family
receptors
evasion
glycan
ligands
their
natural
context,
presented
on
protein
such
as
factors
affecting
fine
binding
specificities,
multivalency
either
at
ligand
receptor
side,
spatial
organization,
finally
current
future
interventions
targeting
Siglec–sialylated
axis
cancer.
Discover Oncology,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: June 28, 2024
Gastric
cancer
(GC)
is
a
malignant
digestive
tract
tumor
with
high
recurrence
rate
and
poor
prognosis.
Fucosylation
important
in
glycosylation,
which
the
key
enzyme
fucosyltransferase
(FUT).
FUT11
member
of
family
has
been
closely
associated
development
multiple
cancers.
However,
specific
relationship
between
GC
prognosis
its
molecular
mechanism
not
fully
studied.
This
study
explored
expression,
clinical
correlation,
role
occurrence
to
deepen
understanding
function.
Expert Review of Proteomics,
Journal Year:
2024,
Volume and Issue:
21(9-10), P. 345 - 365
Published: Oct. 2, 2024
Glycosylation,
the
process
of
glycan
synthesis
and
attachment
to
target
molecules,
is
a
crucial
common
post-translational
modification
(PTM)
in
mammalian
cells.
It
affects
protein's
hydrophilicity,
charge,
solubility,
structure,
localization,
function,
protection
from
proteolysis.
Aberrant
glycosylation
proteins
can
reveal
new
detection
therapeutic
Glyco-biomarkers,
which
help
improve
accurate
early
diagnosis
personalized
treatment.
This
review
underscores
pivotal
role
glycans
glycoproteins
as
source
biomarkers
human
diseases,
particularly
cancer.
PeerJ,
Journal Year:
2024,
Volume and Issue:
12, P. e16785 - e16785
Published: Jan. 22, 2024
Background
Changes
in
protein
glycosylation
have
been
reported
various
diseases,
including
cancer;
however,
the
consequences
of
altered
meningiomas
remains
undefined.
We
established
two
benign
meningioma
cell
lines—SUT-MG12
and
SUT-MG14,
WHO
grade
I—and
demonstrated
glycan
glycosyltransferase
profiles
mucin-type
O-linked
primary
cells
compared
with
malignant
lines—HKBMM
IOMM-Lee,
III.
were
proposed.
Methods
Primary
culture
technique,
morphological
analysis,
immunocytochemistry
used
to
establish
characterize
lines.
The
lines
then
analyzed
using
lectin
cytochemistry.
gene
expression
glycosyltransferases,
mucins,
sialyltransferases,
fucosyltransferases
GEO
database
(GEO
series
GSE16581
)
quantitative-PCR
(qPCR).
Results
Lectin
cytochemistry
revealed
that
terminal
galactose
(Gal)
N-acetyl
galactosamine
(GalNAc)
highly
expressed
(WHO
I)
III).
profile
mucin
types
O-glycosyltransferases
observed
through
experiment
In
database,
C1GALT1-specific
chaperone
(
COSMC
1
MUC1
significantly
increased
(Grade
II
III)
I).
Meanwhile,
lines,
Core
2
β1,6-N-acetylglucosaminyltransferase-2
C2GNT2
was
meningiomas.
investigated
complex
O-glycans
structures
by
determination
sialyltransferases
fucosyltransferases.
found
ST3
β-galactoside
α-2,3-sialyltransferase
4
ST3GAL4)
decreased
while
ST3GAL1,
ST3GAL3
,
α1,3
8
FUT1
FUT8
lines—(HKBMM)—compared
cells—(SUT-MG12
SUT-MG14).
Conclusion
Our
findings
are
first
demonstrate
potential
changes
glycans
meningiomas,
which
may
play
an
essential
role
progression,
tumorigenesis,
malignancy
Frontiers in Immunology,
Journal Year:
2025,
Volume and Issue:
16
Published: Feb. 12, 2025
Background
The
posttranslational
modification
of
cellular
macromolecules
by
glycosylation
is
considered
to
contribute
disease
pathogenesis
in
autoimmune
and
inflammatory
conditions.
In
a
subgroup
patients
with
common
variable
immunodeficiency
(CVID),
the
occurrence
such
complications
associated
an
expansion
naïve-like
CD21
low
B
cells
during
chronic
type
1
immune
activation.
pattern
CVID
has
not
been
addressed
date.
Objective
objective
this
study
was
examine
surface
glycome
dysregulation.
Methods
We
performed
lectin
staining
on
from
peripheral
blood
tonsils,
both
ex
vivo
after
vitro
stimulation.
Additionally,
we
examined
expression
glycosylation-related
genes
RNAseq
,
as
well
naïve
pos
healthy
controls
Results
Unlike
cells,
exhibited
unique
high
levels
α2,6
sialic
acids
fucose.
This
hypersialylation
hyperfucosylation
were
particularly
induced
activation
anti-IgM
interferon-γ
(IFN-γ).
Transcriptome
analysis
suggested
that
possess
comprehensively
reorganised
machinery,
anti-IgM/IFN-γ
having
potential
initiate
these
changes
.
Conclusion
are
hypersialylated
hyperfucosylated.
may
implicate
altered
lectin-ligand
interactions
cell
potentially
affecting
B-cell
function.
These
appear
be
driven
prominent
I
response
complicated
patients.
A
better
understanding
how
influences
function
could
lead
new
therapeutic
strategies.
