CD47 in Osteosarcoma: Correlation with Metastasis and Macrophage-Mediated Phagocytosis DOI Creative Commons
Yunmi Ko,

Seog-Yun Park,

Jong Woong Park

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(22), P. 1862 - 1862

Published: Nov. 10, 2024

CD47 is expressed on cell surfaces and acts as a "don't eat me" signal by interacting with signal-regulatory protein-α the macrophage surface. Some cancer cells express protein can evade phagocytosis. Herein, we evaluated feasibility of targeting for osteosarcoma analyzing its expression patterns, clinicopathological correlations, immunotherapeutic potential. We performed retrospective analysis 24 biopsy samples from patients to investigate correlations between positivity characteristics. was detected in 20.8% samples. correlated metastasis at diagnosis. Patients CD47-positive tumors were older than those CD47-negative tumors. However, not associated sex, tumor size, or histologic response preoperative chemotherapy. In vitro, antibody (B6H12) did affect viability apoptosis. wound-healing assay, inhibited migration cells. Differentiated macrophages exhibited higher phagocytic activity against when pretreated B6H12 compared isotype control. Our preliminary data suggest possible interaction phagocytosis metastasis. A better understanding role necessary develop an innovative approach osteosarcoma.

Language: Английский

Cellular senescence in the tumor with a bone niche microenvironment: friend or foe? DOI Creative Commons

Sajad Alavimanesh,

Negar Nayerain Jazi,

Maedeh Choubani

et al.

Clinical and Experimental Medicine, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 23, 2025

Cellular senescence is understood to be a biological process that defined as irreversible growth arrest and was originally recognized tumor-suppressive mechanism prevents further propagation of damaged cells. More recently, cellular has been shown have dual role in prevention tumor promotion. Senescent cells carry senescence-associated secretory phenotype (SASP), which altered by factors including pro-inflammatory cytokines, chemokines, other proteases, leading the alteration tissue microenvironment. Though would eventually halt cancerous potential cells, SASP contributes environment promoting inflammation, matrix remodeling, cell invasion. The paradox prevention/promotion particularly relevant bone niche microenvironment, where longer-lasting, chronic inflammation promotes formation. Insights into mechanistic understanding provide basis for targeted therapies, such senolytics, aim eliminate senescent or inhibitors, tumor-promoting effects senescence. These therapeutic interventions offer significant clinical implications treating cancer healthy aging.

Language: Английский

Citations

2
Immunotherapy Innovations in the Fight against Osteosarcoma: Emerging Strategies and Promising Progress DOI Creative Commons

Shigao Cheng,

Huiyuan Wang, Xuejia Kang

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(2), P. 251 - 251

Published: Feb. 8, 2024

Immunosuppressive elements within the tumor microenvironment are primary drivers of tumorigenesis and malignant advancement. The presence, as well crosstalk between myeloid-derived suppressor cells (MDSCs), osteosarcoma-associated macrophages (OS-Ms), regulatory T (Tregs), endothelial (ECs) with osteosarcoma cause poor prognosis OS. In addition, consequent immunosuppressive factors favor loss treatment potential. Nanoparticles offer a means to dynamically locally manipulate immuno-nanoparticles, which present promising strategy for transforming OS-TME. Additionally, chimeric antigen receptor (CAR) technology is effective in combating This review summarizes essential mechanisms OS-TME current immune-associated strategies. last part highlights limitations existing therapies offers insights into future research directions.

Language: Английский

Citations

7
IL-1 Family Members in Bone Sarcomas DOI Creative Commons
Lorena Landuzzi, Francesca Ruzzi, Evelin Pellegrini

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(3), P. 233 - 233

Published: Jan. 25, 2024

IL-1 family members have multiple pleiotropic functions affecting various tissues and cells, including the regulation of immune response, hematopoietic homeostasis, bone remodeling, neuronal physiology, synaptic plasticity. Many these activities are involved in pathological processes immunological disorders, tumor initiation progression. Indeed, been described to contribute shaping microenvironment (TME), determining evasion drug resistance, sustain aggressiveness metastasis. This review addresses role sarcomas, particularly highly metastatic osteosarcoma (OS) Ewing sarcoma (EWS), discusses IL-1-family-related mechanisms that play a metastasis development. We also consider therapeutic implications targeting members, which proposed as (i) relevant targets for anti-tumor anti-metastatic drugs; (ii) checkpoints suppression; (iii) potential antigens immunotherapy.

Language: Английский

Citations

4
Integrated analysis of single-cell and bulk transcriptomics reveals cellular subtypes and molecular features associated with osteosarcoma prognosis DOI Creative Commons
Feng Liu, Tingting Zhang, Yongqiang Yang

et al.

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 17, 2025

Osteosarcoma (OS) is the most common primary bone malignancy with variable molecular biology and prognosis. However, our understanding of association between cell types OS progression remains poor. We generated a human atlas by integrating over 110,000 single cells from 17 samples. Multiple machine learning algorithms were applied to develop tumor purity prediction models based on transcriptomic profile OS. The Scissor algorithm gene enrichment analyses conducted delve into cell-intrinsic characteristics linked Moreover, study investigated impact ATF6α in aggressiveness through genetic pharmacological loss function analyses. Lastly, CellChat was employed investigate cell-cell communications. Utilizing high-quality atlas, we identified as prognostic indicator developed robust model. respectively delineated cancer cell- immune associated prognosis at single-cell resolution. Interestingly, activated unfolded protein response (UPR) pathway significantly disease aggressiveness. Notably, emerged top-activated transcription factor for this subcluster. Subsequently, confirmed that markedly progression, while both inhibition impaired survival HOS cells. depicted landscape signal crosstalk UPR-related subcluster other within microenvironment. In summary, work provides novel insights OS, offers valuable resource biomarker discovery treatment strategy development.

Language: Английский

Citations

0
3D Bioprinted Microfluidic Based Osteosarcoma-on-a chip Model as a Physiomimetic Pre-Clinical Drug Testing Platform for Anti-Cancer Drugs DOI
Chitra Jaiswal, Souradeep Dey,

J. Rajendra Prasad

et al.

Biomaterials, Journal Year: 2025, Volume and Issue: unknown, P. 123267 - 123267

Published: March 1, 2025

Language: Английский

Citations

0
LncRNA SPINT1-AS1 enhances the Warburg effect and promotes the progression of osteosarcoma via the miR-135b-5p/PGAM1 axis DOI Creative Commons
Jinyou Wang, Ning Zhang, Yan Wang

et al.

Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 1, 2025

Osteosarcoma (OS) is a malignant bone tumor that originates from interstitial tissues and affects the health of children adolescents. Long noncoding RNAs (lncRNAs) are an intriguing category widely distributed endogenous involved in OS progression, many which remain functionally uncharacterized. Herein, we observed increased expression lncRNA SPINT1-AS1 cell lines. Further analysis confirmed promotes proliferation metastasis cells. Moreover, miR-135b-5p was identified as downstream target SPINT1-AS1. Through bioinformatics analysis, PGAM1 mRNA validated via RIP luciferase reporter assays. could enhance by promoting aerobic glycolysis, acting ceRNA binding to miR-135b-5p, thereby increasing expression. Taken together, these results indicate functions promoter regulates progression through miR-135b-5p/PGAM1 axis.

Language: Английский

Citations

0
Decoding the function of Cancer-Associated Fibroblasts in Osteosarcoma: Molecular pathways, therapeutic approaches and prognostic significance DOI
Chou‐Yi Hsu, Ali G. Alkhathami, Tasneem Ahmed

et al.

Experimental Cell Research, Journal Year: 2025, Volume and Issue: unknown, P. 114612 - 114612

Published: May 1, 2025

Language: Английский

Citations

0
Single-cell RNA sequencing reveals the communications between tumor microenvironment components and tumor metastasis in osteosarcoma DOI Creative Commons
Jiatong Li,

Yang Bai,

He Zhang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 11, 2024

Osteosarcoma is a common type of bone cancer characterized by poor prognosis due to its metastatic nature. The tumor microenvironment (TME) plays critical role in metastasis and therapy response. Therefore, our study aims explore the mechanism osteosarcoma, potentially opening new avenues for treatment.

Language: Английский

Citations

3
The sEVs miR-487a/Notch2/GATA3 axis promotes osteosarcoma lung metastasis by inducing macrophage polarization toward the M2-subtype DOI Creative Commons
Piaopiao Wang, Lei Yang, Jing Dong

et al.

Cancer Cell International, Journal Year: 2024, Volume and Issue: 24(1)

Published: Aug. 31, 2024

Small extracellular vesicles (sEVs) are important mediators of intercellular communication between tumor cells and their surrounding environment. Furthermore, the mechanisms by which miRNAs carried in sEVs regulate macrophage polarization remain largely unknown. To concentrate sEVs, we used traditional ultracentrifugation method. Western blot, NanoSight, transmission electron microscopy were to identify sEVs. determine function sEVs-miR-487a, conducted vivo vitro investigations. The mechanism osteosarcoma M2 macrophages, mediated carrying miR-487a, was validated using luciferase reporter assays, transwell blot analysis. In vitro, enriched miR-487a delivered promoting toward an M2-like type, promotes proliferation, migration, invasion, epithelial-mesenchymal transition (EMT) cells. vivo, facilitate lung metastasis osteosarcoma. Moreover, plasma shown be a potential biomarker applicable for diagnosis. summary, derived from can transferred macrophages via then promote towards type targeting Notch2 activating GATA3 pathway. feedback loop, activation accelerates (EMT), turn This reciprocal interaction activated contributes progression disease. Our data demonstrate new that exosomal-miR-487a is involved development regulating microenvironment (TME).

Language: Английский

Citations

2
Recent advances in nanoagents delivery system-based phototherapy for osteosarcoma treatment DOI

Lichen Ji,

Jiaqing Huang,

Liting Yu

et al.

International Journal of Pharmaceutics, Journal Year: 2024, Volume and Issue: 665, P. 124633 - 124633

Published: Aug. 24, 2024

Language: Английский

Citations

1