Dendritic cell-derived exosomes (Dex): Underlying the role of exosomes derived from diverse DC subtypes in cancer pathogenesis

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 254, P. 155097 - 155097

Published: Jan. 5, 2024

Language: Английский

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Wheat germ agglutinin modified mixed micelles overcome the dual barrier of mucus/enterocytes for effective oral absorption of shikonin and gefitinib

Drug Delivery and Translational Research, Journal Year: 2024, Volume and Issue: unknown

Published: April 24, 2024

Language: Английский

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Study of IL2–330 (rs2069762) gene polymorphism in Egyptian patients with lung cancer: a case-control study

Journal of Immunoassay and Immunochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 14

Published: March 18, 2025

IL2 is one of the key cytokines essential for regulating immune system and inflammation-related carcinogenesis process. Few studies have examined relationship between lung cancer IL2-330 (rs2069762) gene polymorphism, despite several demonstrating that it linked to numerous types. Our study aimed investigate association polymorphism risk explore role in survival outcomes (OS PFS). The was conducted from October 2023 November 2024, including 50 randomly selected patients diagnosed with subjects matched age gender were used as controls this case-control study. assessed using real-time PCR. We found AC genotype associated a notably lower comparison AA (95% CI: 0.06-0.61, p = 0.01). Conversely, CC showed no significant when compared reference genotype. comprehensive distributions among AA, AC, genotypes through Log-Rank (Mantel-Cox) test indicated statistically difference. According our research, significantly higher rate cancer. Further future are needed confirm these findings.

Language: Английский

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IFI30 Knockdown Inhibits ESCC Progression by Promoting Apoptosis and Senescence via Activation of JNK and P21/P16 Pathways

Thoracic Cancer, Journal Year: 2025, Volume and Issue: 16(7)

Published: April 1, 2025

ABSTRACT Background Esophageal squamous cell carcinoma (ESCC) is a prevalent and deadly cancer, making it essential to understand the molecular mechanisms influencing its development prognosis. The role of interferon‐gamma‐inducible protein 30 (IFI30) in antigen processing well‐established, but impact on progression ESCC remains unclear. This study aimed investigate biological function potential IFI30 progression. Methods Public databases, proteomics, immunohistochemistry (IHC) were employed analyze expression. Cell proliferation, migration, invasion evaluated using MTS, colony formation, wound healing, transwell assays. Nude mouse xenograft models established assess effects knockdown vivo. Quantitative proteomics was utilized identify differentially expressed proteins (DEPs) pathways altered by knockdown. apoptosis senescence flow cytometry, SA‐β‐gal staining, reactive oxygen species (ROS) analysis. Results highly correlated with advanced stage poor inhibited vitro suppressed tumor growth DEPs mainly enriched related apoptosis, mitophagy, cellular senescence, lysosome. Furthermore, cells upregulated HRAS expression, increased ROS production, activated JNK signaling pathway, elevated expression P16 P21, thereby promoting senescence. Conclusions suggests that may regulate P21/P16 pathways, exerting pro‐tumorigenic ESCC. could serve as novel target for treatment.

Language: Английский

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Immunomodulatory Effect of Nuciferine on Benzo(a)pyrene (B[a]P)-induced Lung Cancer: A Preclinical Model

Pharmacognosy Magazine, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Background Lung cancer is a deadly type of and the primary cause cancer-related deaths in both males females worldwide. The number lung cases has risen worldwide due to growing population smokers an increase environmental pollutants. Purpose current study was dedicated studying anti-cancer immunomodulatory activities nuciferine against B[a]P-induced mice. Methods initiated BALB/c mice by challenging them with B[a]P for 4 weeks. post- pre-treatment 25 mg/kg given B[a]P-challenged mice’s body weight, tumor incidences, numbers were tabulated. biochemical markers, immunoglobulins (Igs), phase-I -II detoxification enzymes, mitochondrial enzyme assessed using standard techniques. levels inflammatory cytokines biomarkers evaluated assay kits. histopathological analysis conducted tissues experimental Results Both successfully increased weight reduced incidence, numbers. treatment effectively regulated parameters Igs. Furthermore, modulated biomarkers, cytokine levels. findings also proved efficacy cancer. Conclusion present suggest that exhibits chemopreventive properties Therefore, it evident possesses can potentially enhance chemotherapy treatments.

Language: Английский

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The mechanism of cytokine regulation of cancer occurrence and development in the tumor microenvironment and its application in cancer treatment: a narrative review

Translational Cancer Research, Journal Year: 2024, Volume and Issue: 13(10), P. 5649 - 5663

Published: Oct. 1, 2024

The occurrence and development of tumors in human tissues widely depend on their surrounding environment, known as the tumor microenvironment (TME), which comprises various cells, molecules, blood vessels. Through modifications, organization, integration, these elements serve potential therapeutic targets anti-cancer therapy, supporting promoting proliferation, invasion, metabolism cells. Cytokines within TME are responsible for immune cell activation, differentiation, thereby influencing tumor's behavior. This article reviews use cytokines immunotherapy combs network signals that mediate malignancies.

Language: Английский

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Impact of Lithium on the Immune System: An Investigation of T-Cell Subpopulations and Cytokine Responses in Rats

Biological Trace Element Research, Journal Year: 2024, Volume and Issue: unknown

Published: May 3, 2024

Abstract The aim of this study was to investigate the dose-dependent adverse effects long-term dietary lithium administration on specific aspects defense system in rats. Additionally, aimed explore inflammatory activities beyond its recognized anti-inflammatory properties. Forty Wistar Albino rats were involved, which randomly allocated into control and four treatment groups. group received standard rat feed, experimental groups’ diet added 1 g/kg, 1.4 1.8 2.2 g/kg bicarbonate, respectively. CD4+, CD8+, CD161 + cells assessed by flow cytometry. TNF-α, IFN-γ, IL-1β, IL-2 IL-4, IL-6, IL-10 levels measured. proportion CD4 CD4+/CD8 ratio ( P = 0.005 0.038, respectively) reduced with highest dose compared group. data regarding pro-inflammatory cytokines showed a increase serum TNF-α IFN-γ 0.023 0.001, respectively). On other hand, IL-1β decreased manner 0. 001 001, As for cytokines, decrease determined IL-4 level 0.002), while no significant changes noted IL-6 0.507 0.732, In conclusion, adversely impacted cellular system. Furthermore, apart from properties, exhibited cytokine-mediated activities. Therefore, lithium’s potential immune should be considered immunodeficient patients those an status treated high doses lithium.

Language: Английский

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Paracrine Regulation and Immune System Pathways in the Inflammatory Tumor Microenvironment of Lung Cancer: Insights into Oncogenesis and Immunotherapeutic Strategies

Cancers, Journal Year: 2024, Volume and Issue: 16(6), P. 1113 - 1113

Published: March 10, 2024

The intricate interplay between inflammatory processes and the tumor microenvironment (TME) in lung cancer has garnered increasing attention due to its implications for both oncogenesis therapeutic strategies. In this review, we explore recent advances understanding paracrine regulation immune system pathways within TME of cancer. We delve into molecular mechanisms underpinning oncogenesis, highlighting role cell populations, cancer-associated fibroblasts, endothelial cells, as well their interactions through regulated a pattern. Additionally, discuss emerging immunotherapeutic strategies with specific focus on potential leveraging these enhance treatment efficacy

Language: Английский

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Influence of TLR4 signaling on Cannabidiol’s antitumor effectiveness in lung adenocarcinoma cells

Turkish Journal of Biochemistry, Journal Year: 2024, Volume and Issue: 49(3), P. 430 - 438

Published: May 2, 2024

Abstract Objectives Lung cancer remains a predominant type with high incidence and low survival rates. Key challenges in its treatment include impaired cellular mechanisms, notably resistance to apoptosis altered immune responses. A critical aspect this context is the heightened TLR4-mediated signaling, known promote cell survival, metastasis, death, particularly impacting microenvironment regulation. This study focuses on evaluating impact of TLR4 signaling activation potential therapeutic strategies. Methods Our research utilizes cannabidiol (CBD), compound already employed mitigating chemotherapy side effects lung adenocarcinoma, recognized for antitumor properties including antiproliferative, antimetastatic, apoptosis-inducing effects. However, effectiveness CBD cells elevated uncertain. Results findings reveal that combination agonist affects viability, proliferation, cycle progression, apoptosis, gene expression related response extracellular matrix In adenocarcinoma activated TLR4, shows an increased IC 50 value, reflecting reduced antiproliferative capacity. Furthermore, efficacy arresting inducing also compromised. The influence regulation TLR4-activated cells. Conclusions These results indicate significantly diminishes CBD. highlights importance considering future agents like treatment.

Language: Английский

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The Triterpenoid CDDO-Methyl Ester Reduces Tumor Burden, Reprograms the Immune Microenvironment, and Protects from Chemotherapy-Induced Toxicity in a Preclinical Mouse Model of Established Lung Cancer

Antioxidants, Journal Year: 2024, Volume and Issue: 13(6), P. 621 - 621

Published: May 21, 2024

NRF2 activation protects epithelial cells from malignancy, but cancer can upregulate the pathway to promote survival. activators including CDDO-Methyl ester (CDDO-Me) inhibit in preclinical models, suggesting other cell types may anti-tumor activity. However, immunomodulatory effects of remain poorly understood context cancer. To test CDDO-Me a murine model established lung cancer, tumor-bearing wildtype (WT) and Nrf2 knockout (KO) mice were treated with 50–100 mg CDDO-Me/kg diet, alone or combined carboplatin/paclitaxel (C/P) for 8–12 weeks. decreased tumor burden an Nrf2-dependent manner. The combination plus C/P was significantly (p < 0.05) more effective than either drug alone, reducing by 84% WT mice. reduced histopathological grade tumors, higher proportion low-grade tumors lower high-grade tumors. These changes augmented C/P. also protected C/P-induced toxicity improved macrophage T phenotypes mice, expression CD206 PD-L1 on macrophages, decreasing immunosuppressive FoxP3+ CD4+ cells, increasing CD8+

Language: Английский

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